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Effect of lamb age, breed and sex on plasma LH after administration of GnRH.

Male lambs of the Finn, Fingalway and High Fertility breeds aged 4 or 8 weeks of age were injected i.m. with 5, 10, 50, or 100 micrograms GnRH. Plasma LH after GnRH injection increased with age and GnRH dose but there was no effect of breed. There was no interaction among the three factors studied. In a second experiment the response of male and female lambs, aged 8 weeks, to 100 micrograms GnRH was investigated for 5 genetic groups (Finn, Galway Control, Galway Elite, Fingalway and High Fertility). Breed differences in plasma LH of male lambs were not found, which agreed with the result of the first experiment. Breed differences were observed for females, with the lowest values being observed for Finn and High Fertility lambs which represent the two most prolific breeds. This finding suggested breed differences in the development of the hypothalamic-pituitary-ovarian circuit. The difference between Galway Control and Galway Elite lambs was not significant (P greater than 0.10).

Age Factors↗

Evaluation of Methoprene (Altosid) and Diflubenzuron (Dimilin) for control of mosquito breeding in Tezpur (Assam).

Insect growth regulators (IGRs) namely, Isopropyl (E-E)-(RS)-11-methoxy-3,7,11-trimethyldodeca-2, 4-dinoate (Methoprene) and 1-(4-cyclophenyl)-3-(2,6-diflerobenzoyl) urea (Diflubenzuron) were evaluated against mosquito larvae in laboratory as well as in different breeding habitats in Tezpur, Assam. LC90 values of diflubenzuron against Culex quinquefasciatus and Aedes albopictus were 0.0022 and 0.0027 ppm respectively, while it was 0.0027 and 0.0022 ppm respectively in case of methoprene. However, LC50 values of both the IGRs were almost same in case of Ae. albopictus and Cx. quinquefasciatus (varies between 0.0009 and 0.0011 ppm). In case of methoprene, maximum mortality was observed in pupal stage though the exposure was given in all the cases to the III instar larvae. Field trials were conducted in cemented drains, small ponds and ditches. At 0.2 ppm (0.020 kg/ha) both diflubenzuron and methoprene were found to eliminate 92-96 per cent Culex and Anopheles larvae. Methoprene and diflubenzuron were found equally effective for control of mosquito breeding in different breeding habitats and provide better efficacy than conventional larvicides and biocides.

Aedes↗

Antibody response to sheep erythrocytes in Indian native vis-à-vis imported breeds of chickens.

1. A total of 433 birds (7 weeks old) of both sexes belonging to Indian native breeds, including, Aseel, Kadakanath, Naked Neck and Frizzle fowl along with the imported breeds Dahlem Red, White Leghorn, Synthetic dam line broiler (SDL) and Naked Neck broiler were utilised to test the primary antibody response to sheep erythrocytes by haemagglutination test. The effect of genotype (breed), sex and their interactions on antibody response were also studied. 2. The results revealed the presence of natural antibodies in all groups under study. 3. All groups except broilers showed the highest HA titre on day 5 post immunisation, which gradually declined until the end of the experiment (19th day post immunisation). In broilers, the peak HA titre was observed on day 12. 4. Dahlem Red showed the highest response throughout. The lowest antibody response was recorded for broilers except on day 19 post immunisation when it exceeded the White Leghorn value. 5. Amongst the native Indian breeds, the Naked neck had the highest titre on day 5 post immunisation but the Aseel titre was highest on days 12 and 19. 6. Males tended to have higher titres than females in Aseel, Kadakanath, Naked Neck, White Leghorn and Naked Neck broilers whereas Frizzle, Dahlem Red and SDL broilers showed the converse. 7. Statistical analysis revealed significant variation in HA response among the various genetic groups on different days post immunisation. The apparent differences between sexes were not significant. However, interactions between breed and sex were significant on day 5 and 19 post immunisation.

Animals↗

Productive, physiological and biochemical changes in imported and locally born Friesian and Holstein lactating cows under hot summer conditions of Egypt.

Twenty each of healthy lactating Friesian and Holstein cows were studied. They were of similar age and body weight, nonpregnant in their third lactation and 80-100 days post partum. The investigation was carried out for 8 weeks on two farms at the same time during the hot summer season in Egypt. The first farm was in Demietta (North east of the Nile Delta, 31 degrees 40' N) on 20 Friesian cows and the second was in Fakous (East of the Nile Delta, 30 degrees 40' N) on 20 Holstein cows. On each of the two farms, 10 cows were newly imported and 10 were born in Egypt. The average daily milk yield and total milk production in the third lactation of the newly imported cows were significantly higher than those of the locally born cows for both Friesians and Holsteins. At the same time, the T4, T3, urea-N, haematological values and AST enzyme activity in the newly imported cows were significantly lower than those in the locally born ones in both breeds, while the locally born cows showed significantly lower values for rectal temperature and respiration rate, as well as for AST and Alk-P enzyme activities, than the newly imported cows of either breed. Holstein cows surpassed the Friesians in milk production, as well as thyroid hormone secretion and cholesterol, haemoglobin, packed cell volume and erythrocyte count values. The opposite was found for serum total protein, urea-N and creatinine concentrations, leukocyte count and AST, ALT and Alk-P enzyme activities.

Alanine Transaminase↗

Definition of animal breeding goals for sustainable production systems.

What we do is determined by the way we "view" a complex issue and what sample of issues or events we choose to deal with. In this paper, a model based on a communal, cultural, or people-centered worldview, informed by a subjective epistemology and a holistic ontology, is considered. Definitions and interpretations of sustainable agriculture are reviewed. Common elements in published definitions of sustainable agriculture and animal production among those who seek long-term and equitable solutions for food production are resource efficiency, profitability, productivity, environmental soundness, biodiversity, social viability, and ethical aspects. Possible characteristics of future sustainable production systems and further development are presented. The impact of these characteristics on animal breeding goals is reviewed. The need for long-term biologically, ecologically, and sociologically sound breeding goals is emphasized, because animal breeding determined only by short-term market forces leads to unwanted side effects. Hence, a procedure for defining animal breeding goals with ethical priorities and weighing of market and non-market values is suggested. Implementation of non-market as well as market economic trait values in the aggregate genotype, as suggested, may allow for breeding programs that contribute to sustainable production systems. Examples of breeding goals in salmon, cattle, and pigs are given, and the resulting genetic responses are evaluated with respect to economic profit (or costs) and other criteria of sustainability. Important prerequisites for breeding programs for sustainable production are appropriate governmental policies, awareness of our way of thinking, and a more communal worldview informed by a subjective epistemology and a holistic ontology.

Animal Husbandry↗

A lameness scoring system that uses posture and gait to predict dairy cattle reproductive performance.

Lameness has contributed to reproductive inefficiency and increased the risk of culling in dairy cows. We developed a 5-point lameness scoring system that assessed gait and placed a novel emphasis on back posture. Our objective was to determine if this system predicted future reproductive performance and the risk of culling. The study was conducted at a commercial dairy farm with a history of declining reproductive efficiency and an increasing prevalence of lameness. A total of 66 primipara and pluripara calved, received an initial lameness score, and completed their 60-d voluntary waiting period. The overall prevalence of lameness (mean lameness score >2) was 65.2%. Scoring continued at 4-wk intervals and ceased with conception or culling. The percentage of cows confirmed pregnant and culled was 77.3 and 22.7, respectively. For each reproductive endpoint, a 2 x 2 table was constructed with lameness score >2 as the positive risk factor and either performance greater than the endpoint mean or being culled as the positive disease or condition. Positive and negative predictive values, relative risk, Chisquare statistic and regression analysis were used to evaluate the data. The positive predictive values for days to first service, days open, breeding herd days, services per pregnancy and being culled were 58, 68, 65, 39 and 35%, respectively. Similarly, the negative predictive values were 79, 96, 100, 96 and 100%, respectively. Except for one reproductive endpoint, the total number of services, all linear regressions were significant at P < 0.01. Having a lameness score >2 predicted that a cow would have extended intervals from calving to first service and to conception, spend or be assigned to (explained herein) more total days in the breeding herd, require more services per pregnancy and be 8.4 times more likely to be culled. We believe that this lameness scoring system effectively identifies lame cows. Observation of the arched-back posture in a standing cow (> or =LS 3) should trigger corrective interventions.

Journal Article↗

Nulliparous versus primiparous crossbred females for beef.

Feedlot and carcass traits of nulliparous and primiparous females representing eight breed types, including Bos taurus and Bos indicus x Bos taurus crosses, were evaluated. Nulliparous females (heifers) were in the feedlot for 4 mo; primiparous females (heiferettes) were fed for 2 1/3 mo after their calves were weaned at 6 mo of age. Heifers averaged higher (P less than .01) in dressing percentage, percentage of kidney fat, carcass grade (P less than .10), and color of lean (P less than .05) compared with heiferettes. Heiferettes exceeded the nulliparous group in feedlot ADG (P less than .01), fat thickness (P less than .05), and percentage of steak, roast, and bone (P less than .01). Parity effects on carcass weight, longissimus area, marbling, pH, and shear force value were not statistically significant. Dam breed types differed in several traits, including marbling (P less than .05) and percentage of steak (P less than .10), roast (P less than .01), and bone (P less than .01). Dam breed x parity interactions were nonsignificant. Results show that beef derived from heiferettes is competitive with heifer beef.

Adipose Tissue↗

Economic value of pregnancy in dairy cattle.

The objective of this study was to estimate the value of pregnancy for dairy cows. Effects of the stage of gestation, stage of lactation, lactation number, milk yield, milk price, replacement heifer cost, probability of pregnancy, probability of involuntary culling, and breeding decisions were studied. A bioeconomic model was used, and breeding and replacement decisions were optimized. A general Holstein herd in the United States was modeled. The average value of a new pregnancy was $278. The value of a new pregnancy increased with days in milk early in lactation but typically decreased later in lactation. Relatively high-producing cows and first-lactation cows reached greater values, and their values peaked later in lactation. The average cost of a pregnancy loss (abortion) was $555. The cost of a pregnancy loss typically increased with gestation length. Sensitivity analyses showed that an increased probability of pregnancy, an increased persistency of milk yield, and a smaller replacement heifer cost greatly reduced the average value of a pregnancy. The value of a new pregnancy was negative for relatively high-producing first-lactation cows when persistency of lactation and the probability of pregnancy were increased. Breeding was delayed when the value of pregnancy was negative. Changes in milk price, absolute milk yield, and probability of involuntary culling had less effect on the value of pregnancy. The value of pregnancy and optimal breeding decisions for individual cows were greatly dependent on the predicted daily milk yield for the remaining period of lactation. An improved understanding of the value of pregnancy may support decision making in reproductive management when resources are limited.

Abortion, Veterinary↗

The effects of breed, neonatal age and pregnancy on the plasma copper status of goats in Oman.

There is a history of copper deficiency in grazing Omani livestock and the copper status of three economically important goat breeds, Jabal Akhdar (JA), Batina (B) and Dhofari (D) were therefore compared in October/November (cool season) and June (dry season) in a penned flock given a plentiful dietary supply of copper. In the cool season, 62 lactating does (5 JA, 12 B and 33 D), their 0-5-day-old kids (17 JA, 19 B and 22 D) and 25 dry does (17 JA, 5 B and 3 D) were blood sampled. In the dry season, the does sampled were either barren (15 JA, 16 B and 13 D) or pregnant (9 JA, 13 B and 33 D). The samples were analysed for total copper (TCu) and trichloroacetic acid (TCA)-soluble copper (TCA-sol Cu). There were no effects of breed on TCu or TCA-sol Cu in the cool season, the overall means being 0.75 (SE 0.049) and 0.59 (SE 0.052) mg/L. The mean TCu was low in kids at birth (0.59 mg/L) but had increased to 0.86 mg/L by 4 days of age (p < 0.001); breed differences were found (p < 0.002), the pooled values for JA, B and D being 0.77, 0.59 and 0.68 (SE 0.033-0.044) mg/L, respectively. By the dry season, the mean TCu had risen in barren does to 0.96 (0.045) mg/L but not in pregnant does (0.76 (0.047) mg/L: p < 0.002) and breed differences had emerged, the mean for D being 20% lower than those for JA and B (p < 0.05). The highest TCu values were found in the 7-month-old kids in June ( 1.17 (0.039) mg/L) but the breeds did not then differ. Some effects on TCA solubility were found but were considered unreliable. Breed effects may have been diminished by the generous supply of copper and early stage of lactation studied.

Age Factors↗

Studies on age specific leukocyte and lymphocyte counts in normal Friesian, Red Danish and Jersey cattle in Denmark.

To determine reference values for leukocyte and lymphocyte counts of the most common cattle breeds in Denmark, especially regarding the evaluation procedure in the leukosis control work, blood samples from normal cattle of all ages within the Friesian, the Red Danish and the Jersey breeds were collected, transported and counted as in the routine leukosis control programme. Evaluation of breed- and age specific counts showed numerically small, but statistically significant differences in mean values of leukocytes and lymphocytes between on the one hand Jersey cattle and on the other hand Red Danish and Friesian cattle. However, it was found that the key values used in the leukosis control work are generally in good agreement with the calculated 99.87 percentiles of all age- and breed specific lymphocyte count distributions, which were approximated with logarithmic normal distribution curves.

Age Factors↗

Economic values for resistance to gastrointestinal helminths in meat sheep in Kenya.

Economic values for resistance to gastrointestinal (GI) helminths in meat sheep were estimated based on previously published genetic and phenotypic parameters in a selection index objectively optimizing gains on yearling weight (YW). A two-trait index of YW and faecal egg count (FEC) were considered when accounting only for tangible roles of sheep production using individual records for index calculation (scheme 1) or including individual, sire, dam and male paternal half-sib information (scheme 2). Schemes 3 and 4 were similar to schemes 1 and 2, respectively, but accounted for both tangible and intangible roles of sheep production. Economic values within each scheme were estimated for five breeding objectives as follows: (i) index response in YW equals response from single trait selection, (ii) index response in YW is maximum, (iii) the level of FEC was held constant, (iv) A predetermined response of -0.22 (x1000) eggs per gram was assumed and (v) Monetary value of response in FEC is minimum. All estimated economic values were negative, and were Kenya Shillings (KSh) -34.90 (US dollar -0.50), 0.00, -92.20 (US dollar -1.32), -153.31 (US dollar -2.19) and -47.90 (US dollar -0.68), respectively, for options 1-5 in scheme 1. In all schemes the breeding options ranked 4, 3, 5 and 1 in descending order; however, options 1 and 5 exchanged positions in schemes 2 and 4. Economic values were responsive to changes in heritability of FEC and genetic correlation between the two traits. The magnitude of the economic values reflects the importance of including resistance to GI helminths in the breeding objectives for meat sheep in different production systems.

Animals↗

Haematological parameters of the Polish Primitive Horses.

Average values of chosen haematological parameters have been described in a population of 90 clinically normal Polish Primitive Horses. The comparison of the results with values given in literature for horses as a whole and for other breeds enabled us to state that Polish Primitive Horses' blood is characterized by relatively low values of RBC, PCV, segmented neutrophils and monocytes, and high percentage of lymphocytes. It was demonstrated that values of some blood parameters of examined animals are similar to typical results obtained for primitive and cold-blooded horses. Moreover, it was proved that age, sex, and season have influence upon many blood parameters' values of the Polish Primitive Horses. More precise evaluation of results received with that breed will be possible when that influence is taken into consideration.

Aging↗

Genetic architecture and evolution of stripe rust resistance uncovered using diverse panels of wheat lines and North American Puccinia striiformis f. sp. tritici isolates.

Screening global wheat germplasm with a diverse collection of pathogen races expands the catalog of novel Yr loci and identifies new sources of broad-spectrum resistance against evolving Pst populations. Newly emerging highly virulent races of Puccinia striiformis f. sp. tritici (Pst) often defeat deployed resistance genes (Yr), highlighting the need for novel sources of durable resistance. A global diversity panel of 377 spring wheat (Triticum aestivum L.) lines was screened for all-stage resistance (ASR) against a panel of diverse 20&#xa0;Pst isolates at the seedling stage and for adult-plant-stage resistance (APR) against natural mix of field races. Genome-wide association mapping identified 77 unique Yr loci. Of these, 34 overlapped with the previously mapped 1150 Yr loci, confirming the robustness of our GWAS results, while 43 were likely novel. Comparison of the nine adult-plant-stage Yr loci mapped in our study with known APR genes identified only one overlap, with Yr29. Except for Yr29, APR genes Yr18 and Yr36 were detected at low frequencies, indicating that resistance in our panel may arise from less characterized or novel sources. Two wheat lines, lacking widely effective Yr5 and Yr15 alleles, exhibited resistance to all 20&#xa0;Pst races at the seedling stage and natural field races at the adult stage, suggesting that they may carry novel, broad-spectrum ASR alleles. Wheat improvement had no effect on the frequency of ASR alleles but resulted in a threefold increase in the frequency of APR alleles, suggesting that the latter were subjected to more consistent breeding selection over time. Our findings underscore the value of combined screening of diverse germplasm with diverse pathogen races to identify novel sources of broad-spectrum resistance for breeding stripe rust resistant cultivars.

Triticum↗

Analysis of variability in nutrient digestibilities in broiler chickens.

1. Forty commercial broiler chickens from two different breeding origins were used for individual measurements of growth performance from d 7 to d 21. From d 21 to d 24 a balance experiment was carried out for the measurement of metabolisable energy (ME), digestibilities of lipids, starch and amino acids, viscosity of excreta water-extract, and amount of water loss. After this, the weight and length of the different parts of the gastrointestinal tract were measured for each bird. During the experiment, the birds were fed with an experimental diet containing 5 g/kg of guar gum in order to increase the variability of responses. 2. There were no significant (P < 0.05) differences between the two breeds in 21 d live weight, food:gain ratio, ME value or the digestibilities of lipids, starch and total amino acids. Significant (P < 0.05) differences between the two breeds were observed for caecal size, excreta guar gum degradation measured using viscosity measurements, and vent score. 3. Pooling all the individual data, individual ME values were correlated (r2 = 0.33) with individual food:gain ratios, which shows that a great part of ME variation was associated with individual variation. 4. Several significant (P < 0.05) correlations were observed between individual digestibilities and individual anatomical characteristics of the gastrointestinal tract. The most significant were concerned with the duodenum weight:length ratio correlated with ME (r = 0.474) and the caeca weight:body weight ratio correlated with guar gum degradation (r = 0.495). Covariance analyses were carried out when correlations were significant and did not show significant interactions with the breeding origin of chickens.

Animal Feed↗

[The native domestic animal breeds of Japan].

During the last decades some domestic animal breeds have spread to all parts of the world. In general, consideration is given to these breeds only; on the other hand, autochthonous breeds of various countries are hardly known. These, however, can be valuable gene-reserves, and moreover, they represent a significant cultural value. In Japan there are several domestic animal breeds which are almost unknown in Central Europe. They are presented verbally and by means of illustration, and their breeding history is dealt with as well. The purpose of this study is to point out the importance of these breeds within the country and to make clear the extent of the danger of their extinction.

Animals↗

Economic evaluation of alternative crossbreeding systems involving four breeds of swine. I. The simulation model.

A static, deterministic computer model, programmed in Microsoft Basic for IBM PC and Apple Macintosh computers, was developed to calculate production efficiency (cost per kg of product) for nine alternative types of crossbreeding system involving four breeds of swine. The model simulates efficiencies for four purebred and 60 alternative two-, three- and four-breed rotation, rotaterminal, backcross and static cross systems. Crossbreeding systems were defined as including all purebred, crossbred and commercial matings necessary to maintain a total of 10,000 farrowings. Driving variables for the model are mean conception rate at first service and for an 8-wk breeding season, litter size born, preweaning survival rate, postweaning average daily gain, feed-to-gain ratio and carcass backfat. Predictions are computed using breed direct genetic and maternal effects for the four breeds, plus individual, maternal and paternal specific heterosis values, input by the user. Inputs required to calculate the number of females farrowing in each sub-system include the proportion of males and females replaced each breeding cycle in purebred and crossbred populations, the proportion of male and female offspring in seedstock herds that become breeding animals, and the number of females per boar. Inputs required to calculate the efficiency of terminal production (cost-to-product ratio) for each sub-system include breeding herd feed intake, gilt development costs, feed costs and labor and overhead costs. Crossbreeding system efficiency is calculated as the weighted average of sub-system cost-to-product ratio values, weighting by the number of females farrowing in each sub-system.

Animal Husbandry↗

Analysis of 22 heterologous microsatellite markers for genetic variability in Indian goats.

The genetic variability of 22 heterologous microsatellite markers was analyzed in two Indian goat breeds, namely Bengal and Chegu. The heterozygosity, polymorphism information content (PIC), and probability of identity of two individuals were calculated for all microsatellite loci in both the breeds. The observed number of alleles varied between 4 and 13 at the studied microsatellite loci. The evaluated microsatellite loci exhibited high mean heterozygosity of 0.69 +/- 0.11 and 0.66 +/- 0.07 in Bengal and Chegu goats, respectively. The mean PIC values of the studied loci in these breeds were 0.79 +/- 0.08 and 0.78 +/- 0.05, respectively. The probability of identity of two random individuals from different breeds, taking into account, all the 22 microsatellite loci was as low as 5.523 x 10(-40). On the basis of these results, we propose that these microsatellite markers may be used with reliability for studying genetic diversity and for identification of individuals in Indian goat breeds.

Alleles↗

Natal origins of migratory monarch butterflies at wintering colonies in Mexico: new isotopic evidence.

Each year, millions of monarch butterflies from eastern North America migrate to overwinter in 10-13 discrete colonies located in the Oyamel forests of central Mexico. For decades efforts to track monarch migration have relied on observations and tag-recapture methods, culminating with the discovery of the wintering colonies in 1975. Monarch tag returns from Mexico, however, are few and primarily from two accessible colonies, and therefore tag-recapture techniques have not quantified natal origins or distinctiveness among monarch populations at wintering sites. Such information would be invaluable in the conservation of the monarch and its migration phenomenon since the wintering sites currently are threatened by habitat alteration. Here we show that stable hydrogen (deltaD) and carbon (delta13C) isotope ratios of wintering monarchs can be used to evaluate natal origins on the summer breeding range. Stable-hydrogen and carbon isotopic values of 597 wintering monarchs from 13 wintering roost sites were compared with isotopic patterns measured in individuals at natal sites across their breeding range over a single migration cycle. We determined that all monarch wintering colonies were composed of individuals originating mainly from the Midwest, United States, thereby providing evidence for a panmictic model of wintering colony composition. However, two colonies showed more northerly origins, suggesting possible priority colonies for conservation efforts.

Journal Article↗