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[The characteristics of the color perceptive space in protanomals].

Color vision of three protanomal subjects was studied by means of direct paired comparison technique using 25 colors with different brightness. It was shown that the characteristics of their color vision could be completely and adequately described in the frames of the four-dimensional spherical model of color perception. The spatial axes could be identified as the two color-opponent mechanisms (red-green and blue-yellow) and the two achromatic mechanisms (brightness and darkness). Deformation of the color axes in protanomals (as compared with the normal trichromatics) was demonstrated in the "red" and "yellow" spectral region. The visual disturbance in protanomal subjects involves not only color but also achromatic mechanisms. This is manifested in the a deformation of perceptual brightness scale. In comparison with normal trichromatic subjects, the protanomals perceive the red and adjacent colors as achromatic while green, yellow-green, and orange as more bright but low-saturated colors.

Adolescent↗

Three-color labeling method for flow cytometric measurement of cytogenetic damage in rodent and human blood.

Experiments described herein were designed to evaluate the performance characteristics of a flow cytometry-based system that scores the incidence of peripheral blood micronucleated reticulocytes (MN-RETs). These procedures represent the continued refinement of a previously reported anti-CD71-based method (Dertinger et al. [1996]: Mutat Res 371:283-292), with the following modifications: incorporation of a third fluorescent label to exclude platelets from the MN-RET region, and use of a CD71-associated fluorescence thresholding technique to increase data acquisition rates. Mouse, rat, and human blood samples were analyzed using both the previously described two-color procedure (anti-CD71-FITC and propidium iodide) and a newly developed three-color technique (which adds an antiplatelet-PE antibody). The rodent specimens were also evaluated by standard microscopy procedures (acridine orange staining). Mouse blood was collected via heart puncture of vehicle- and 5-fluorouracil-treated CD-1 mice; blood samples from saline-treated Sprague-Dawley rats were collected from the tail vein and via heart puncture. Rodent blood samples were analyzed by both the two- and three-color methods. Human blood specimens, obtained via arm venipuncture from cancer patients undergoing radiation therapy, were analyzed for MN-RETs using the two-color method. Subsequently, blood samples from a single chemotherapy patient were analyzed by both the two- and three-color methods. Finally, the chemotherapy patient blood samples and blood samples from 15 healthy volunteers were evaluated at very high densities in conjunction with a CD71-associated fluorescence thresholding technique. Results of these investigations showed that data from mouse blood analyzed by the two- and three-color procedures correlated well with microscopy data (r values = 0.917 and 0.937 for the two- and three-color methods, respectively); all three methods confirmed the genotoxicity of 5-FU. Data from rat tail vein samples showed improved reproducibility with the three-color technique, but no significant difference between the two techniques was seen with the heart puncture specimens. Human blood analyzed according to the two-color procedure produced unreliable results, as platelets and platelet aggregates impacted the rare MN-RET scoring region. The three-color technique effectively overcame this problem and produced reproducible measurements that fell within expected ranges. For human blood analyses, the high cell density/CD71-thresholding technique provided significant improvements over the low-density technique, as it allowed data acquisition to occur approximately six times faster with no loss of sensitivity.

Adult↗

Left ventricular ejection fraction measured with Doppler color flow mapping techniques.

To determine if left ventricular (LV) ejection fraction (EF) can be accurately measured from the color Doppler examination, 11 patients (aged 0.4 to 22 years) underwent 2-dimensional and color Doppler examinations within 24 hours of cardiac catheterization. With use of a biplane Simpson's rule, LV end-diastolic volume, end-systolic volume and EF were measured from cineangiograms, 2-dimensional echocardiograms and color Doppler examinations. The 2-dimensional echocardiographic and color Doppler measurements were obtained from apical 4-chamber and long-axis views. The color Doppler examinations were performed by placing the color sector over the left ventricle only. The velocity scale was set at the lowest possible Nyquist limit (less than 0.17 m/s), and the highest possible carrier frequency was used to obtain this limit. With these settings, all flow signals in the LV chamber were aliased so that the entire chamber was filled with mosaic color Doppler signals. Motion of the surrounding LV walls gave rise to nonaliased (pure red-blue) signals. With use of an off-line analysis system equipped with a color frame grabber, the border of the mosaic color flow area was traced to obtain volumes and EF. End-diastolic and end-systolic volumes measured with color Doppler correlated well with those measured from 2-dimensional echocardiography (r = 0.99, standard error of the estimate [SEE] = 11.9 ml; r = 0.99, SEE = 4.4 ml, respectively) and cineangiography (r = 0.92, SEE = 16.8 ml; r = 0.90, SEE = 9.9 ml, respectively).(ABSTRACT TRUNCATED AT 250 WORDS)

Adolescent↗

Color analysis method for estimating the oxygen saturation of hemoglobin using an image-input and processing system.

A color analysis method which enables both qualitative and quantitative analyses of an object's color was developed. The method uses a color image-input and processing system composed of a 3-tube video camera and a digital image analyzer, which quantizes a color image into values of red, green, and blue brightness, then processes these values. We introduced a spectrophotometric principle by the Beer-Lambert law, and were able to establish a color model to analyze an object's color. In the coordinate space based on our color model, the hue of the object's color is represented by the direction from the origin, and the density by the distance from the origin. This new method was used to analyze the colors of hemoglobin solutions at various oxygen saturations and concentrations. The results agreed with the known conditions, indicating the validity of the model and its usefulness for quantitative as well as qualitative analyses of color.

Algorithms↗

Hormonal control of polymorphic and sexually dimorphic coloration in the lizard Sceloporus undulatus erythrocheilus.

This study investigated the influence of sex steroids on the expression of seasonally labile and sexually dimorphic coloration in the red-lipped plateau lizard. The responses of ventral blue and polymorphic facial coloration to exogenous steroid administration were assessed. Though facial color is usually dimorphic in nature, males and females did not differ in the degree to which they expressed facial coloration following administration of testosterone (T). Both sexes developed significantly more ventral blue coloration than controls, but a fundamental dimorphism in intensity, favoring males, was maintained throughout the experiment. These results indicate that facial coloration is under activational influence of T. The ventral blue coloration is probably organized at sexual maturation, thus manifesting the more vivid expression in males. Males of this subspecies are polymorphic for facial coloration. Administration of T did not cause any lizards to change hue, whether their color morph was yellow or orange or lacked the facial pigments altogether. The difference among color morphs is probably not controlled by differences in circulating steroid concentrations and is hypothesized to be under genetic control.

Animals↗

Neuroradiologic MR applications with multiparametric color composite display.

The purpose of this article is to demonstrate the application of a PC-based multiparameter full color composite display technique of MR images of 14 selected patients with neuropathology while assessing the ability of this technique to display clinically important neuroanatomic and neuropathologic tissues. Using a PC with a 386 microprocessor and full color 24-bit graphics display capabilities, custom and commercially available image-processing softwares were applied to spatially aligned multiparameter proton density, T1-weighted (with and/or without gadolinium-DTPA) and T2-weighted MR image sets obtained from 14 patients with known neuropathology to generate intensity-based color composites. Quantitative color channel applications were used to assess the ability of this technique to differentiate anatomically and pathologically confirmed tissue types into unique color regions within the full color spectrum display in each patient case. Based on the results of pathologic correlation and quantitative color imaging analysis, the application of full color composite generation techniques to multiple MR images of selected neuropathology cases represents a viable technique for displaying diagnostically relevant tissue contrast information in one color image. With this technique, it is possible to generate composites that simultaneously display uniquely color-coded neuroanatomic and neuropathologic tissue information within the context of partially natural-appearing images.

Adult↗

Phase-related body-color polyphenism in hatchlings of the desert locust, Schistocerca gregaria: re-examination of the maternal and crowding effects.

The mechanism controlling the body color of hatchlings was studied for the desert locust, Schistocerca gregaria. A pheromonal factor secreted by gregarious female adults into the foam plugs of egg pods has been suggested to cause darkening in their progeny. We re-examined the role of this maternal factor by washing or separating eggs at deposition. Eggs produced by crowd-reared female adults were washed with saline or separated individually without being washed immediately after deposition and the body color of the hatchlings from them was compared with that from the eggs unwashed and kept in the egg pod until hatching. Most hatchlings were dark and no significant difference was found in the proportions of dark- and light-colored hatchlings between the treatments and controls. Likewise, eggs separated before the foam plug deposition produced dark-colored hatchlings as in the un-separated controls. These results demonstrated that neither washing nor separation of eggs at deposition affected the hatchling body coloration. The variation in hatchling body color was correlated closely to the body weight at hatching, indicating that hatchling body color had been determined maternally. Green hatchlings reared under crowded conditions remained green until the second stadium at which black patterns were induced. It was concluded that body color at hatching has been determined maternally and crowding during the first nymphal stadium influences nymphal body color but its effect is not manifested until the second stadium. The present study casts doubts on the presence of a recently suggested pheromonal factor on the color of the hatchlings.

Animals↗

Objective measurement of dental color for age estimation by spectroradiometry.

Age-related changes in tooth color have been described previously, however, the use of dental color for age estimation in forensic odontology has been limited due to the difficulty of measuring color objectively. This study presents an objective method for determining dental color to estimate the age of an individual. Dentine color in 250 teeth from patients ranging in age from 10 to 89 years was determined by spectroradiometry. Color measurements were performed as suggested in the CIE 1931 (International Commission on Illumination). Chromaticity coordinates (x, y, z), luminance (Y), whiteness index (WIC, Z%, WIC) and yellowness index (YI) were obtained. Correlations between these colorimetric variables and aging were established by linear regression analyses. All the variables fit the mathematical model with correlation coefficients ranging from 0.53 to 0.75. This method of color measurement produced an expected associated error of calibration averaging 13.7 years about the mean estimated values, at a 70% level of confidence. Two different multiple regression models for dental age estimation were tested, and variables that made the greatest contributions to age calculation were identified. To determine the effect of postmortem interval on tooth color and its influence in age estimation, 37 teeth obtained from human skeletal remains buried during an interval ranging from 21 to 37 years were also studied. In this material, the correlation between age and dental color measured by spectroradiometry was weaker than in fresh extracted teeth. It is concluded that determination of dentine color by spectroradiometry is a potentially useful objective method to estimate age in forensic studies in combination with other methods.

Adolescent↗

PC-based multiparameter full-color display for tissue segmentation in MRI of adnexal masses.

OBJECTIVE: Our purpose was to apply full-color composite generation methods to multiparameter MRI to assess the ability of the technique to quantitatively segment clinically important anatomic and pathologic tissues. MATERIALS AND METHODS: With use of a personal computer with a 386 microprocessor and full-color (24 bit) graphics display capabilities, custom and commercially available image-processing softwares were applied to spatially aligned multiparameter SE MR image sets obtained from six patients undergoing diagnostic work-up for suspected adnexal or pelvic masses to generate intensity-based color composites. To quantitatively assess the ability of this technique to differentially segment anatomically and pathologically confirmed tissue types into unique color regions within the full-color spectrum, color image analysis was performed on the multiparameter color composites within each patient case, and the results were compared using 95% confidence intervals. RESULTS: Based on the results of pathologic correlation and color image analysis, the generation of full-color composites represents a feasible technique for compressing the diverse tissue contrast data present in multiparameter MR images of adnexal masses. CONCLUSION: With this technique, it is possible to generate composites that simultaneously display uniquely color-coded anatomic and pathologic tissue information within the context of partially natural-appearing images.

Adnexal Diseases↗

Effects of common origin and common environment on nestling plumage coloration in the great tit (Parus major).

Carotenoids cannot be synthesized by birds and thus have to be ingested with food, suggesting that carotenoid-based plumage coloration is environmentally determined. However signaling functions ascribed to plumage imply that plumage coloration is the outcome of an evolutionary process based on genetic variation. By means of a cross-fostering design we show significant effects of both a common rearing environment and the brood from which a nestling originally came from (common origin) on the plumage coloration of nestling great tits (Parus major). This demonstration of origin-related variation in carotenoid-based plumage coloration suggests that the observed variation of the trait has a partial genetic basis. Consistent with environmental determination of this trait, we also found a significant positive correlation between the color saturation of nestlings and their foster-father's plumage. There was no significant correlation between nestling plumage coloration and the food quantity provided to the nestlings by the male, the female, or both parents. This suggests that the nestling-foster father correlation arises by the carotenoid quantity ingested rather than the food quantity per se. No significant nestling-true father correlation was found, which suggests that nestling plumage coloration did not indirectly evolve due to sexual selection. Consistent with this result there was no significant correlation between the nestling's plumage color and its coloration as a breeding adult the following year, suggesting that nestling plumage color is a different trait than the first year plumage.

Animals↗

Correlated evolution of conspicuous coloration and body size in poison frogs (Dendrobatidae).

Conspicuous coloration is often used in combination with chemical defenses to deter predators from attacking. Experimental studies have shown that the avoidance inducing effect of conspicuous prey coloration increases with increasing size of pattern elements and with increasing body size. Here we use a comparative approach to test the prediction from these findings, namely that conspicuous coloration will evolve in tandem with body size. In our analysis, we use a previously published mitochondrial DNA-based phylogeny and comparative analysis of independent contrasts to examine if evolutionary shifts in color pattern have been associated with evolutionary changes in body size in aposematic poison frogs (Anura: Dendrobatidae). Information on body size (snout to vent length) and coloration were obtained from the literature. Two different measures of conspicuousness were used, one based on rankings by human observers and the other based on computer analysis of digitized photographs. The results from comparative analyses using either measure of coloration indicated that avoidance inducing coloration and body size have evolved in concert in poison frogs. Results from reconstruction of character change further indicate that the correlated evolution of size and coloration has involved changes in both directions within each of the different clades of the phylogenetic tree. This finding is consistent with the hypothesis that selection imposed by visually guided predators has promoted the evolution of larger body size in species with conspicuous coloration, or enhanced evolution of conspicuous coloration in larger species.

Animals↗

Color Doppler mapping of aortic regurgitation in aortic stenosis: comparison with angiography.

Color flow Doppler mapping has become the principal noninvasive method used for the qualitative grading of aortic regurgitation (AR). However, the performance of the color Doppler method in patients with AR accompanying aortic stenosis (AS) has not been studied. We therefore compared results of color Doppler and semiquantitative angiographic grading of AR in 32 patients with AS (mean valve area = 0.7 cm2) undergoing supravalvular aortography in the course of cardiac catheterization. Color Doppler demonstrated AR in all 27 patients who had AR by aortography. As expected, neither the maximal jet area nor the jet length discriminated patients by angiographic grade. The best correlation between color Doppler and aortography occurred when the ratio of maximal jet height (JH) to left ventricular outflow tract (LVOT) height was used to grade AR on a scale of 0-4. Four of 5 patients without AR by aortography had either absent or grade 1 AR by color Doppler. Although there was considerable overlap of color Doppler grades in patients with 1+ AR by aortography, grade 3 or 4 AR by color Doppler was always associated with III+ or IV+ AR by aortography. Thus, color Doppler sensitively depicts AR in patients with AS, and the ratio of JH to LVOT height by color Doppler correctly identifies patients with III+ or IV+ AR by aortography. Methods for distinguishing among milder grades require further evaluation.

Aortic Valve↗

Chromatic interaction between egg pigmentation and skin chromatophores in the nuptial coloration of female two-spotted gobies.

In two-spotted gobies (Gobiusculus flavescens Fabricius 1779), females develop an orange belly as they approach sexual maturity. Bright belly coloration is preferred by males and has been suggested to act as a female ornament. This coloration is unusual in that it originates partly from pigmentation of the abdominal skin but also from strongly pigmented gonads directly visible through the skin. In addition, females have been observed to temporarily become more colourful during courtship and competition. To understand how gonad and skin pigmentation interact in this nuptial coloration, the potential for colour modification via regulation of skin chromatophores was investigated. Noradrenaline caused aggregation of chromatophore pigment and was used to experimentally reduce the contribution of skin chromatophores to the nuptial coloration. Chromatophore pigment aggregation caused bellies to become less colourful and abdominal skin biopsies to become less colourful and more transparent. There was a strong positive relationship between belly coloration and the coloration of the underlying gonads. This shows that belly coloration honestly reflects egg pigmentation, mainly because the transparency of the abdominal skin allows other fish to see the gonads directly. Interestingly, when noradrenaline caused pigment to aggregate and thereby increased the transparency of the skin, the relationship between belly and gonad coloration weakened. We conclude that female G. flavescens have a potential to use skin chromatophores to rapidly alter their nuptial coloration, thereby affecting the efficacy with which information about gonad coloration is conveyed.

Animals↗

Color rendering properties of interior lighting influenced by a switchable window.

Switchable windows are being installed into modern buildings. We report on how the variable transmittance of gasochromic and electrochromic switchable windows changes the color rendering properties of the daylight passing through these windows. We present a series of color rendering examples. Correlated color temperature and color rendering indices are insufficient to describe the color properties of this filtered light, as these indices are beyond the applicability limits. We obtain more reliable results using the color shifts (deltaE) of different objects. We find that as the transmittance of the window changes, each surface color moves along a path of the CIELAB space in the same direction for both switchable units. The direction and distance moved differs among test-color samples. The appearance of an array of colored objects is strongly distorted at higher coloration states of the windows.

Journal Article↗

[Chromatic study of all-ceramic crown--IPS Empress: difference of color by manufacturing technique and cements].

PURPOSE: In recent years,aesthetic appearance and function are called for and all-ceramic crowns are spreading. By choosing an all-ceramic crown the problem of metal ceramics is avoided. There are difficulties of color tone reproducibility of cervical margin and darkness of gingival margin. We examined IPS Empress also in various all-ceramic crowns. IPS Empress has high permeability a ceramic ingot of various color tones and excellent color tone reproducibility of natural teeth. Generally a layering technique is used for an anterior tooth and the staining technique is used for a molar. However the details are unknown We examined how differences of manufacturing method and cement affect the color tone of all ceramics clinically. METHODS: Two kinds of Empress crown were fabricated for a 27 year-old woman's upper left-side central incisors:the staining technique of IPS Empress and the layering technique of IPS Empress II. Various try-in pastes(transparent opaque white white and yellow) of VariolinkII of the IPS Empress System were used for cementing. Color was measured using a spectrophotometer CMS 35FS. The L*a*b* color system was used for showing a color. The right-side central incisors on the opposite side of the same name teeth were used for comparison. RESULTS: We analyzed the color difference (DeltaE* ab)with a natural tooth. Consequently when it had no cement of staining technique and was tranceparent small values were obtained. CONCLUSIONS: It is considered that the color tone can be adjusted by color cement. It is effective to use the staining technique for an anterior tooth crown depending on the case. The crown manufactured using the layering technique is not easily influenced by cement. The crown manufactured by the staining technique tends to be influenced by cement.

English Abstract↗

Improved pediatric multidetector body CT using a size-based color-coded format.

OBJECTIVE: CT technique should be adjusted while scanning infants and children. One format that has proven successful in simplifying pediatric care and reducing medical error is the size-based, color-coded Broselow-Luten pediatric system. This color-coded system can serve as a format for CT protocols. The purpose of this investigation was to compare variation (or error) occurrence and technologist preference for conventional and color-coded formats for pediatric multidetector body CT protocols. MATERIALS AND METHODS: Multidetector CT examinations were set up using either a conventional or a color-coded format for a period of 6 weeks each. Variations (errors) from protocol parameters (including tube current, detector configuration, table speed, and IV contrast media dose) were tabulated. Qualitative assessment consisted of a survey of CT technologists (n = 20) for preference in six areas related to ease of use and clarity of the formats. RESULTS: There were 44 CT examinations (n = 30 infants and children) in the conventional group and 55 CT examinations (n = 31 infants and children) in the color-coded protocol format group. Overall, the number of errors was significantly less in the color-coded group (p < 0.01), with a significantly lower error percentage in individual parameters affecting radiation dose, including tube current, detector configuration, and table speed (p < 0.05). In all areas, the color-coded format was preferred over the conventional format (p < 0.0003). CONCLUSION: Color-coded CT formatting is an extension of a clinical color-coded system. This system provides an easy, expeditious, consistent, and preferable format for general pediatric body CT protocols. Most importantly, the color-coded system can reduce variations (errors) in the radiology department.

Adolescent↗

Adaptive and maladaptive utilization of color cues by patients with mild to moderate Alzheimer's disease.

The ability to utilize color information was investigated in 12 patients with mild to moderate probable Alzheimer's Disease (DAT) and in 12 age- and gender-matched control subjects. All subjects underwent testing of visual acuity and color vision before being tested with a cognitive task consisting of four conditions (no color, color as attention enhancer, color as valid cue, color as distracter). Although the groups did not differ in visual acuity or color vision, patients with DAT were less accurate than controls in all four conditions of the cognitive task. Both groups performed best with color as a valid cue and worst with color as distracter, but condition had a significantly stronger effect on patients than on controls. It is concluded that color is a potent stimulus attribute for patients with DAT.

Journal Article↗

Perspective: the evolution of warning coloration is not paradoxical.

Animals that are brightly colored have intrigued scientists since the time of Darwin, because it seems surprising that prey should have evolved to be clearly visible to predators. Often this self-advertisement is explained by the prey being unprofitable in some way, with the conspicuous warning coloration helping to protect the prey because it signals to potential predators that the prey is unprofitable. However, such signals only work in this way once predators have learned to associate the conspicuous color with the unprofitability of the prey. The evolution of warning coloration is still widely considered to be a paradox, because it has traditionally been assumed that the very first brightly colored individuals would be at an immediate selective disadvantage because of their greater conspicuousness to predators that are naive to the meaning of the signal. As a result, it has been difficult to understand how a novel conspicuous color morph could ever avoid extinction for long enough for predators to become educated about the signal. Thus, the traditional view that the evolution of warning coloration is difficult to explain rests entirely on assumptions about the foraging behavior of predators. However, we review recent evidence from a range of studies of predator foraging decisions, which refute these established assumptions. These studies show that: (1) Many predators are so conservative in their food preferences that even very conspicuous novel prey morphs are not necessarily at a selective disadvantage. (2) The survival and spread of novel color morphs can be simulated in field and aviary experiments using real predators (birds) foraging on successive generations of artificial prey populations. This work demonstrates that the foraging preferences of predators can regularly (though not always) result in the increase to fixation of a novel morph appearing in a population of familiar-colored prey. Such fixation events occur even if both novel and familiar prey are fully palatable and despite the novel food being much more conspicuous than the familiar prey. These studies therefore provide strong empirical evidence that conspicuous coloration can evolve readily, and repeatedly, as a result of the conservative foraging decisions of predators.

Adaptation, Physiological↗