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Thermal inactivation kinetics of Salmonella spp. within intact eggs heated using humidity-controlled air.

The heat resistance of six strains of Salmonella (including Enteritidis, Heidelberg, and Typhimurium) in liquid whole egg and shell eggs was determined. Decimal reduction times (D-values) of each of the six strains were determined in liquid whole egg heated at 56.7 degrees C within glass capillary tubes immersed in a water bath. D-values ranged from 3.05 to 4.09 min, and significant differences were observed between the strains tested (alpha = 0.05). In addition, approximately 7 log10 CFU/g of a six-strain cocktail was inoculated into the geometric center of raw shell eggs and the eggs heated at 57.2 degrees C using convection currents of humidity-controlled air. D-values of the pooled salmonellae ranged from 5.49 to 6.12 min within the center of intact shell eggs. A heating period of 70 min or more resulted in no surviving salmonellae being detected (i.e., an 8.7-log reduction per egg).

Animals↗

Survival of Salmonella on tomatoes stored at high relative humidity, in soil, and on tomatoes in contact with soil.

Salmonellosis has been linked to the consumption of several types of raw fruits and vegetables, some of which may have been contaminated with Salmonella before harvesting. The objectives of this study were to investigate water and soil as reservoirs of Salmonella for the contamination of mature green tomato fruits. Salmonella survived for at least 45 days in inoculated moist soil. The population of Salmonella on tomatoes in contact with soil increased by 2.5 log10 CFU per tomato during storage for 4 days at 20 degrees C and remained constant for an additional 10 days. The number of cells inoculated on tomatoes decreased by approximately 4 log10 CFU per tomato during storage for 14 days at 20 degrees C and 70% relative humidity. Fruits in contact with inoculated soil for 1 day at 20 degrees C harbored Salmonella only near or on the skin surface. More Salmonella cells were observed in stem scar and subsurface areas of tomatoes as the time of storage increased. PCR fingerprinting revealed that among five Salmonella serotypes in the inoculum, Salmonella Montevideo was the most persistent on tomatoes in contact with inoculated soil and on spot-inoculated tomatoes, followed by Salmonella Poona and Salmonella Michigan. The results of this study demonstrate that an enhanced green fluorescent protein marker can be used to detect cells and monitor the growth of Salmonella in the presence of other microorganisms. Observations on the infiltration of Salmonella into tomato tissues support the contention that preharvest contact of produce with contaminated water or soil exacerbates problems associated with the postharvest removal of pathogens or their accessibility to treatment with sanitizers.

Bacterial Typing Techniques↗

Increased formaldehyde in jet engine exhaust with changes to JP-8, lower temperature, and lower humidity irritates eyes and respiratory tract.

BACKGROUND: Formaldehyde (FA) in exhaust from F-4 aircraft with low smoke combustor(LSC) J79 engines has been reported to be of sufficient concentration to cause irritation. It has also been noted that eye and respiratory irritation became more frequent and severe after the fuel was changed from JP-4 to JP-8. The present sturdy investigated the effect of jet fuel and power setting on formaldehyde concentrations in the exhaust. We also investigated the exposure to formaldehyde among pilots and flight line personnel. METHODS: The exhaust from LSC J79 engines using different types of fuel (JP-8 and JP-4) was sampled 50 m behind the engine at different power settings in July (summer season in Japan) and February (winter season ). It was also sampled at 75% power settings using JP-8 in July. RESULTS: At an idle power setting, the FA concentration was higher in the exhaust of engines using JP-8 (1.31 ppm in July and 2.78 ppm in February) than in engines using JP-4 (0.95 ppm in July and 1.84 ppm in February). The FA concentration increased as both ambient temperature and relative humility decreased in the sampling atmosphere. The FA concentration of JP-8 fuel at an idle power setting (65%) was higher than that at a 71.5% power setting (1.32 ppm and 0.86 ppm, respectively). CONCLUSIONS: The FA concentrations in LSCJ79 engine exhaust varies depending on the type of fuel, engine power settings, and ambient air conditions. A high FA concentration at ground level due to a change in the fuel type, low temperature, and humidity, causes frequent severe eye respiratory irritation.

Aerospace Medicine↗

[The balance of harmful trace contaminants between the air humidity condensate and air in a simulator of the Mir orbit station moisture condensation unit].

Subject of the investigation was the balance of harmful trace contaminants (HTC) between the air moisture condensate and air in a simulator of the MIR moisture condensation unit. Experiments involved various classes of water-solvent compounds including alcohols (C1-C4), ketons (C1-C2), aldehydes (C1-C2), fatty acids (C2-C4), esters (acetates C4-C6), and ammonium. For most of the compounds, removal efficiency correlates with air humidity and virtually does not depend on the HTC concentration within the range of 0.25 to 59.1 mg/m3.

Acetates↗

Thermophotovoltaic (TPV) properties of hornet cuticle as dependent on relative humidity.

This paper deals with the thermophotovoltaic (TPV) properties of the cuticle of the Oriental hornet as assessed over time under different regimens of relative humidity (RH). The tests were run at two levels of RH, namely, 30% vs. 90%. Each experiment entailed measuring the cuticular voltage and current in the dark as compared to under illumination (white light = 700 Lux), and at a temperature range of 20-30 degrees C. It was found that increase in the RH level boosts the current values by 2-3 orders of magnitude; contrariwise, the voltage values rise by about three times with drop in the RH. At high RH, the changes in current become rhythmical and each cycle of warming-cooling assumes a distinctly cyclic pattern. Under illumination, the current decreases, the polarity reverses and the resistance increases. The obtained results are describable by a model of electric conductance upon a surface, in this case the hornet cuticle; the findings are also discussed and compared with similar phenomena recorded from other substances possessing the properties of organic semiconductors.

Animals↗

Effect of temperature and relative humidity on spinning behaviour of silkworm (Bombyx mori.L).

The rate of spinning of silkworm larva (Bombyx mori.L) was slow at 22 degrees C and fast at 38 degrees C. The time taken for completion of cocoon was longest at 98 +/- 2% and least at 40 +/- 2% RH. However, good quality cocoons were spun at 22 degrees C and 65 +/- 5% RH, hence it would be advantageous to maintain this temperature and relative humidity at the time of cocoon spinning in the case of CB race of silkworm.

Animals↗

Potato growth in response to relative humidity.

Potato plants (Solanum tuberosum L. cvs. Russet Burbank, Norland, and Denali) were grown for 56 days in controlled-environment rooms under continuous light at 20C and 50% or 85% RH. No significant differences in total plant dry weight were measured between the humidity treatments, but plants grown under 85% RH produced higher tuber yields. Leaf areas were greater under 50% RH and leaves tended to be larger and darker green than at 85% RH.

Biomass↗

High relative humidity increases yield, harvest index, flowering, and gynophore growth of hydroponically grown peanut plants.

Growth chamber experiments were conducted to study the physiological and growth response of peanut (Arachis hypogaea L.) to 50% and 85% relative humidity (RH). The objective was to determine the effects of RH on pod and seed yield, harvest index, and flowering of peanut grown by the nutrient film technique (NFT). 'Georgia Red' peanut plants (14 days old) were planted into growth channels (0.15 x 0.15 x 1.2 m). Plants were spaced 25 cm apart with 15 cm between channels. A modified half-Hoagland solution with an additional 2 mM Ca was used. Solution pH was maintained between 6.4 and 6.7, and electrical conductivity (EC) ranged between 1100 and 1200 microS cm-1. Temperature regimes of 28/22 degrees C were maintained during the light/dark periods (12 hours each) with photosynthetic photon flux (PPF) at canopy level of 500 micromoles-m-2s-1. Foliage and pod fresh and dry weights, total seed yield, harvest index (HI), and seed maturity were greater at high than at low RH. Plants grown at 85% RH had greater total and individual leaflet area and stomatal conductance, flowered 3 days earlier and had a greater number of flowers reaching anthesis. Gynophores grew more rapidly at 85% than at 50% RH.

Arachis↗

[The phorophyte occupation patterns by vascular epiphyte species in the High Orinoco tropical humid forest, Amazonas State, Venezuela].

By international agreement (Austria-Venezuela), an experimental area was selected for canopy ecology studies. A tower crane was installed that allows direct access to the canopy of 1.5 ha area of the forest. This is a quantitative study of the phorophyte occupation patterns by vascular epiphyte species in an Amazon tropical humid forest. The forest of Surumoni consists roughly of four arboreal strata, i.e., emergent (conspicuous), superior, middle and lower. The largest proportion of trees occurs in the lower diametric classes (smaller DBH), and the total distribution approaches an "inverted J" curve. The most abundant tree species in the three strata is Goupia glabra (Celastraceae); making this forest a case where a single arboreal species dominants. Mean of epiphyte individuals per occupied phorophyte is too variable, depending on phorophyte species and showing no correlation with occupied tree percentage. The species Eschweleira parviflora (Lecythidaceae) represented in the study area by a single individual of the superior stratum with high density of vascular epiphytes, indicates that this support species is particularly favorable for the establishment epiphytic flora.

Ecosystem↗

[Species composition and microbial contamination of atmospheric humidity condensate and potable water from the Mir water supply systems during Mir main missions 4 through 27].

Analyzed were quantitative and qualitative characteristics, and processes of formation and transformation of microflora in the atmospheric humidity condensate (AHC) and potable water (PW) regenerated from the condensate by the long-operating system (SRV-K) aboard space station Mir. The paper presents data on species composition of microflora of AHC, and PW regenerated by SRV-K in the period from 1989 through to 1999. The following microbial species were identified in AHC: Alcaligenes (14.8%), Bacillus (3.7%), Citrobacter (7.4%), Clostridium (11.1%), Enterobacter (7.4%), Proteus (7.4%), Pseudomonas (18.5%), and Staphylococcus (100%). There were two PW cocks in SRV-K--"hot" and "cold". "Hot" PW microflora included Alcaligenes (15%), Bacillus (5%), Citrobacter (20%), Clostridium (5%), Enterobacter (15%), Proteus (10%), Pseudomonas (15%), and Staphylococcus (75%). "Cold" PW microflora included Aeromonas (14.3%), Alcaligenes (21.4%), Bacillus (7.1%), Citrobacter (7.1%), Clostridium (7.1%), Enterobacter (7.1%), Moraxella (7.1%), Pseudomonas (7.1%), and Staphylococcus (78.5%). Microbial content averaged 8.9 x 10(2) +/- 1.3 (0.01-4) x 10(3) CFU/ml in condensate and 1.5 +/- 0.1 (0-3.0) CFU/ml in regenerated potable water.

Atmosphere↗

Atrazine and metribuzin sorption in soils of the Argentinean humid pampas.

Laboratory studies were conducted to determine the influence of surface and subsurface properties of three representative soils of the humid pampas of Argentina on atrazine and metribuzin sorption. Atrazine and metribuzin sorption isotherms were constructed for each soil at four depths. Sorption affinity of herbicides was approximated by the Freundlich constant (K(f)), distribution coefficient (Kd), and the normalized Kd based on organic carbon content (K(oc)). Multiple regression of the sorption constants against selected soil properties indicated that organic carbon content (OC) and silt were related positively and negatively, respectively, to atrazine K(f) coefficient (r2 = 0.93), while Kd coefficient of atrazine was related positively to organic carbon content and negatively to both silt and cation exchange capacity (CEC) (r2 = 0.96). For metribuzin, only organic matter content was related positively to Kr coefficient (r2 = 0.51). Lower K(f) values for atrazine were obtained for all soils with increasing depth, indicating lesser sorption at greater depths. Metribuzin sorption was quite similar across all depths. Sorption constant K(f) of atrazine ranged from 2.06 to 7.82, while metribuzin K(f) values ranged from 1.8 to 3.52 and were lower than atrazine for all soils and depths, indicating a greater leaching potential across the soil profile.

Adsorption↗

Effect of occlusive wound dressing supplemented with antiphlogistic and analgesic agents on plasma beta-endorphin in hot and humid environments: a study in pigs.

OBJECTIVE: To study the changes of plasma beta-endorphin (beta-EP) in pigs with traumatic injury after occlusive wound dressing supplemented with antiphlogistic and analgesic agents in hot and humid environments (HHE). METHODS: Traumatic models were established in 10 pigs, 5 of which received antiphlogistic- and analgesic-supplemented occlusive dressings of the wounds (experiment group, EG), while the rest pigs were assigned to control group (CG) to receive routine wound management. The pigs in both groups were then exposed to artificial HHE and at different time points during the exposure, the plasma beta-EP level, respiratory frequency and heart rates were measured respectively. RESULTS: The plasma beta-EP concentration of EG was significantly lower than that of CG (P <0.01) after the injury, but in both groups, the levels before the injury were similar to those measured at hour 8 during HHE exposure and at hour 24 following the injury. The variation range of the respiratory frequency and heart rates during HHE exposure were significantly smaller in EG than CG (P <0.01). CONCLUSION: This supplemented occlusive wound dressing can help restrain the peak of plasma beta-EP level and the variation range of respiratory frequency and heart rates of pigs exposed to HHE.

Analgesics↗

Predicting increases in skin temperature using heat stress indices and relative humidity in helicopter pilots.

We used measurements of heat stress in the cockpit of Bell 206 and 212 helicopters manned by a crew of two to predict changes in the skin temperatures of 50 pilots over a 1-h ground standby period. Cockpit wet bulb globe temperatures increased from 28.1 +/- 5.6 degrees C to 32.9 +/- 3.9 degrees C over the 1-h ground standby period. Skin temperatures increased from 34.7 +/- 1.1 to 35.4 +/- 1.1 degrees C, while heart rate increased from 79.4 +/- 9.6 to 82.6 +/- 9.8 bpm. Cockpit wet bulb temperatures explained 26.9% of the variance of the increase in skin temperature (P = 0.0002). Wet bulb globe temperatures, discomfort index and dry bulb temperatures did not improve the predictive value of wet bulb temperatures alone. Relative humidity was not significantly associated with the increase in skin temperature. We conclude that wet bulb temperatures predict changes in skin temperature as well as other heat stress indices. Furthermore, despite high cockpit temperatures, pilots experienced only minimal strain during the 1-h ground standby period. Extrapolation of our results to the effects on body temperature of higher degrees of heat stress and physical activity is unwarranted and deserves further study.

Adult↗

Gonado-modulatory effects of high humidity is pineal dependent in Indian palm squirrel Funambulus pennanti (Wroughton).

F. pennanti presented a clear biphasic pineal mediated seasonal sexual cycle. This sexual cycle was essentially characterised by a very short period of sexual quiescence with an arrest of spermatogenesis during October-November. A small but clear decrease in sexual activity was also observed during March-April. This decrease in sexual activity, however, had no quantifiable effect on spermatogenesis. Sexual recrudescence was observed from December-January. The testes remained sexually active from January till September. Almost an inverse relationship was observed between pineal and testicular weight. Pinealectomy, however, prevented naturally induced gonadal regression during both the periods, i.e. September-November and February-April. Exposure of animals to high RH (80 +/- 4%) during sexually active phase induced a steep regression in testicular weight of sham-operated animals even in the presence of gonad stimulatory long photoperiod (16L:8D) and high temperature (40 degrees +/- 5 degrees C) while exposure of animals to moderate RH (65 +/- 5%) during sexual regression phase partially prevented testicular regression even in presence of inhibitory short photoperiod (11L:13D) and normal environmental temperature (30 degrees +/- 5 degrees C). Pinealectomized animals, neither exhibited testicular regression in February-March nor had involuted testes in September-October, thus, suggesting that the effect of humidity is mediated via the pineal gland.

Animals↗

[An overview of basic and clinical studies on the management of craniocerebral gunshot injury in hot and humid environment].

The author systemically reviewed the pathophysiological characteristics of craniocerebral gunshot injuries in a hot and humid environment, and such an environmental factor was considered to inflict a secondary brain insult as one of the main causes for early morbidity and mortality of patients after the injury. Management of such injuries remains an important subject in the researches of military medicine, the results of which can be of great significance to reduce the mortality rate of the injuries.

Blood-Brain Barrier↗

[Effect of hot and humid environment on bone marrow and spleen of mice exposed to gamma irradiation].

OBJECTIVE: To study the effect of hot and humid environment (HHE) on the bone marrow and spleen in mouse exposed to gamma irradiation. METHODS: After exposure to gamma irradiation at the dosage of 6.5 Gy, the mice were subjected to treatment in HHE for 1 h or at room temperature (RT) respectively. After exposure to HHE, the mice in HHE group were kept at RT. The bone marrow and spleen of the mice in both RT and HHE groups were examined at 1, 3, 5, 9 and 15 days after irradiation for bone marrow cellularity, DNA content in the bone marrow cells, and spleen/body weight ratio. RESULTS: Compared with the RT group, mice in the HEE group showed aggravation of decreases in bone marrow cells, DNA content and spleen/body weight ratio. CONCLUSIONS: HHE aggravates radiation injury of the bone marrow and spleen in mice.

Animals↗

Temperature and humidity within the clothing microenvironment.

The present study investigates clothing microenvironment conditions that may develop during prolonged exposure of workers to a hot environment. Five subjects were exposed to a linear increase in ambient temperature from 20-40 degrees C over a 90-min period, and then remained at 40 degrees C for an additional 90 min. During the exposures, subjects were clad in four types of helicopter personnel suits (Gore-Tex, Cotton Ventile, Nomex/Insulite, and Nomex/Neoprene), incorporating both dry-suit and wet-suit designs. Continuous assessment was made of skin temperature, rectal temperature, and of microenvironment temperature, relative humidity, and vapor pressure (T mu, RH mu, and VP mu) 8 mm from the surface of the skin. Results indicate that although microenvironment temperatures were similar among suits and slightly lower than that of the environment, the RH mu and VP mu were much greater than those of the ambient air. The Nomex/Insulite and Nomex/Neoprene suits showed the highest VP mu, of which only the Nomex/Insulite resulted in significantly greater increases in rectal temperature, likely due to complete covering of the body with the impermeable insulite component. The present study demonstrates the need to discern between the ambient conditions and the conditions encountered next to the skin when protective clothing is worn.

Adult↗

Influence of inoculum density of Verticillium dahliae, temperature and relative humidity on epidemics of verticillium wilt of cotton in northern Iran.

During 1992--2003, frequency of Verticillium dahliae propagules, disease incidence and severity of verticillium wilt of cotton were determined in several cotton growing fields in Golestan province, northeastern Iran. Inoculum density varied among fields and different years ranging between 2-47 propagules/g of air-dried soil with an average of 18.96+/-0.73. In addition, the pattern of diseased plants varied with type of field and year. Simple regression analysis showed a linear relationship between inoculum density of V. dahliae at planting time on one hand, disease incidence and severity for all years on the other. The straight line model described the increase in disease intensity index over the accumulated physiological time from sowing. The number of days above 28 degrees C (T) and the area under relative humidity (RH) had significant effects on inoculum density in soil (MS) and final disease development (Y) and fitted the Y = 65.840 - 0.0034 RH + 0.57 MS - 1.7T model with R2 = 0.859 and significant F-function (p<0.0001).

Climate↗