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PubMed1983

Rat hippocampal neurons in culture: responses to electrical and chemical stimuli.

Intracellular activity was recorded from dissociated rat hippocampal neurons maintained in tissue culture conditions for 4-6 wk. The cells developed dense interconnections and had typical morphological characteristics similar to hippocampal neurons in situ. The recorded neurons possessed similar electrophysiological properties to those observed in situ or in a slice preparation. Their input resistance (42 M omega), resting membrane potential (-60 mV), membrane time constant (16.2 ms), total electrotonic length (0.92), and spike size (68.3 mV) were similar to values obtained in hippocampal cells in a slice. The connections among adjacent neurons were largely inhibitory. The inhibitory postsynaptic potentials (IPSPs) had longer durations than excitatory postsynaptic potentials (EPSPs) when these were detected. Synaptic delay varied between 0.3 and 3.0 ms. There were no electrotonic connections among neurons. Reciprocal connections were common. Most neurons reacted to acetylcholine (ACh) by an increase in frequency of spontaneous EPSPs, action-potential discharges, and IPSPs. Concurrently, there was a marked reduction in the magnitude of the evoked PSPs tested in pairs of cells. This effect is probably presynaptic to the recorded neurons. A statistical analysis of quantal properties of the synaptic interactions among neurons revealed that ACh causes a reduction of magnitude of PSPs by reducing the number of releasing elements (m). This effect is different from the reduction of evoked PSPs caused by postsynaptic depolarization.

Acetylcholine↗

PubMed1996

Physiological properties of anatomically identified basket and bistratified cells in the CA1 area of the rat hippocampus in vitro.

Basket and bistratified cells form two anatomically distinct classes of GABAergic local-circuit neurons in the CA1 region of the rat hippocampus. A physiological comparison was made of intracellularly recorded basket (n = 13) and bistratified neurons (n = 6), all of which had been anatomically defined by their efferent target profile (Halasy et al., 1996). Basket cells had an average resting membrane potential of -64.2 +/- 7.2 vs. -69.2 +/- 4.6 mV in bistratified cells. The latter had considerably higher mean input resistances (60.2 +/- 42.1 vs. 31.3 +/- 10.9 M Ohms) and longer membrane time constants (18.6 +/- 8.1 vs. 9.8 +/- 4.5 ms) than basket cells. Differences were also apparent in the duration of action potentials, those of basket cells being 364 +/- 77 and those of bistratified cells being 527 +/- 138 microseconds at half-amplitude. Action potentials were generally followed by prominent, fast after-hyperpolarizing potentials which in basket cells were 13.5 +/- 6.7 mV in amplitude vs. 10.5 +/- 5.1 in bistratified cells. The differences in membrane time constant, resting membrane potential, and action potential duration reached statistical significance (P < 0.05). Extracellular stimulation of Schaffer collateral/commissural afferents elicited short-latency excitatory postsynaptic potentials (EPSPs) in both cell types. The average 10-90% rise time and duration (at half-amplitude) of subthreshold EPSPs in basket cells were 1.9 +/- 0.5 and 10.7 +/- 5.6 ms, compared to 3.3 +/- 1.3 and 20.1 +/- 9.7 ms in bistratified cells, the difference in EPSP rise times being statistically significant. Basket and bistratified EPSPs were highly sensitive to a bath applied antagonist of non-N-methyl-D-aspartate (NMDA) receptors, whereas the remaining slow-rise EPSP could be abolished by an NMDA receptor antagonist. Increasing stimulation intensity elicited biphasic inhibitory postsynaptic potentials (IPSPs) in both basket and bistratified cells. In conclusion, basket and bistratified cells in the CA1 area show prominent differences in several of their membrane and firing properties. Both cell classes are activated by Schaffer collateral/commissural axons in a feedforward manner and receive inhibitory input from other, as yet unidentified, local-circuit neurons.

Action Potentials↗

PubMed1990

Action of an irreversible acetylcholine esterase inhibitor, soman, on muscarinic hyperpolarization in cat bladder parasympathetic ganglia.

1. Intracellular recording techniques were used to examine the action of an irreversible acetylcholine esterase (AChE) inhibitor, soman, on the hyperpolarizations mediated through muscarinic cholinoceptors in cat bladder parasympathetic neurones. 2. Soman (0.1-10 microM) depressed the amplitude and prolonged the duration of the muscarinic slow inhibitory postsynaptic potential (s-i.p.s.p.) elicited by a preganglionic tetanus (40 Hz for 1 s) in the presence of mecamylamine (20 microM), phentolamine (1 microM) and caffeine (1 mM), in a dose-dependent manner. The effect of soman on the amplitude of the s-i.p.s.p. was partially reversible, while the effect on the duration was irreversible. 3. Soman hyperpolarized the membrane and decreased input resistance, but this effect could not account for soman-induced inhibition of the s-i.p.s.p. 4. Soman depressed the amplitude and prolonged the duration of a muscarinic hyperpolarization induced by pressure application of acetylcholine (ACh) in the presence of mecamylamine, phentolamine and caffeine. The time course of this effect paralleled that on the synaptically-evoked muscarinic s-i.p.s.p. 5. A reversible AChE inhibitor, pyridostigmine (10-100 microM), also depressed the amplitude and prolonged the duration of a muscarinic hyperpolarization induced by either preganglionic stimulation or ACh pressure application. These actions were reversible, and not accompanied by a significant change in membrane potential or input resistance. 6. The inhibitory action of soman (1 microM) on the muscarinic hyperpolarization was prevented by pyridostigmine (10 microM), but not by atropine (1 microM). 7. These results demonstrate that soman prolongs not only the muscarinic hyperpolarization, but also inhibits its amplitude through a postsynaptic action, probably through AChE inhibition, in cat bladder parasympathetic neurones.

Acetylcholine↗

PubMed1994

Physiology and morphology of complex spiking neurons in the guinea pig dorsal cochlear nucleus.

Intracellular recordings from the dorsal cochlear nucleus have identified cells with both simple and complex action potential waveforms. We investigated the hypothesis that cartwheel cells are a specific cell type that generates complex action potentials, based on their analogous anatomical, developmental, and biochemical similarities to cerebellar Purkinje cells, which are known to discharge complex action potentials. Intracellular recordings were made from a brain slice preparation of the guinea pig dorsal cochlear nucleus. A subpopulation of cells discharged a series of two or three action potentials riding on a slow depolarization as an all-or-none event; this discharge pattern is called a complex spike or burst. These cells also exhibited anodal break bursts, anomalous rectification, subthreshold inward rectification, and frequent inhibitory postsynaptic potentials (IPSPs). Seven complex-spiking cells were stained with intracellular dyes and subsequently identified as cartwheel neurons. In contrast, six identified simple-spiking cells recorded in concurrent experiments were pyramidal cells. The cartwheel cell bodies reside in the lower part of layer 1 and the upper part of layer 2 of the nucleus. The cells are characterized by spiny dendrites penetrating the molecular layer, a lack of basal dendritic processes, and an axonal plexus invading layers 2 and 3, and the inner regions of layer 1. The cartwheel cell axons made putative synaptic contacts at the light microscopic level with pyramidal cells and small cells, including stellate cells, granule cells, and other cartwheel cells in layers 1 and 2. The axonal plexus of individual cartwheel cells suggests that they can inhibit cells receiving input from either the same or adjacent parallel fibers and that this inhibition is distributed along the isofrequency contours of the nucleus.

Action Potentials↗

PubMed1995

Correlation between dendrodendritic synapses of adrenergic type and synaptically evoked hyperpolarization in the sympathetic ganglion of adult rats.

Intracellular recording and labeling with biocytin followed by electron microscopic observation were used to examine the nature and the morphological basis of a synaptically evoked hyperpolarization following spikes in the rat superior cervical ganglion neurons. A large hyperpolarization (the amplitude > 8 mV; the duration > 1 s following spikes) was elicited by repetitive stimulation of the preganglionic nerves in 8% of cells examined (n = 50). The alpha 2-adrenoceptor antagonist, yohimbine, reversibly attenuated the hyperpolarization, without affecting spikes. A nicotinic antagonist, hexamethonium, blocked both the hyperpolarization and spikes. Atropine had no effect of these responses. Electron microscopic observation of dendrites of these cells revealed that they received synaptic inputs of adrenergic type besides a cholinergic one from the preganglionic axons. Some dendrites served as presynaptic elements. These results strongly suggest that the hyperpolarization is an inhibitory postsynaptic potential and that this disynaptic response to the preganglionic stimulation is mediated mainly by two transmitters, acetylcholine and noradrenaline that are released from axodendritic and dendrodendritic synapses, respectively. We conclude that there appears to be an adrenergic inhibitory local circuit that modulates cholinergic transmission in the sympathetic ganglia.

Adrenergic alpha-1 Receptor Antagonists↗

PubMed1995

Temporal information transformed into a spatial code by a neural network with realistic properties.

Neurons exhibit a wide range of properties in addition to postsynaptic potential (PSP) summation and spike generation. Although other neuronal properties such as paired-pulse facilitation (PPF) and slow PSPs are well characterized, their role in information processing remains unclear. It is possible that these properties contribute to temporal processing in the range of hundreds of milliseconds, a range relevant to most complex sensory processing. A continuous-time neural network model based on integrate-and-fire elements that incorporate PPF and slow inhibitory postsynaptic potentials (IPSPs) was developed here. The time constants of the PPF and IPSPs were estimated from empirical data and were identical and constant for all elements in the circuit. When these elements were incorporated into a circuit inspired by neocortical connectivity, the network was able to discriminate different temporal patterns. Generalization emerged spontaneously. These results demonstrate that known time-dependent neuronal properties enable a network to transform temporal information into a spatial code in a self-organizing manner--that is, with no need to assume a spectrum of time delays or to custom-design the circuit.

Electric Stimulation↗

PubMed2004

Mechanism of irregular firing of suprachiasmatic nucleus neurons in rat hypothalamic slices.

The mechanisms of irregular firing of spontaneous action potentials in neurons from the rat suprachiasmatic nucleus (SCN) were studied in hypothalamic slices using cell-attached and whole cell recording. The firing pattern of spontaneous action potentials could be divided into regular and irregular, based on the interspike interval (ISI) histogram and the membrane potential trajectory between action potentials. Similar to previous studies, regular neurons had a firing rate about >3.5 Hz and irregular neurons typically fired about <3.5 Hz. The ISI of irregular-firing neurons was a linear function of the sum of inhibitory postsynaptic potentials (IPSPs) between action potentials. Bicuculline (10-30 microM) suppressed IPSPs and converted an irregular pattern to a more regular firing. Bicuculline also depolarized SCN neurons and induced bursting-like activity in some SCN neurons. Gabazine (20 microM), however, suppressed IPSPs without depolarization, and also converted irregular activity to regular firing. Thus GABAA receptor-mediated IPSPs appear responsible for irregular firing of SCN neurons in hypothalamic slices.

Action Potentials↗

PubMed2006

Different forms of homeostatic plasticity are engaged with distinct temporal profiles.

Global changes in network activity have been reported to induce homeostatic plasticity at multiple synaptic and cellular loci. Though individual types of plasticity are normally examined in isolation, it is their interactions and net effect that will ultimately determine their functional consequences. Here we examine homeostatic plasticity of both inhibition and intrinsic excitability in parallel in rat organotypic hippocampal slices. As previous studies have not examined inhibitory plasticity using a functional measure, inhibition was measured by the ability of evoked inhibitory postsynaptic potentials (IPSPs) to suppress action potentials, as well as IPSP amplitude. We show that manipulations of network activity can both up- and downregulate functional inhibition, as well as intrinsic excitability. However, these forms of plasticity are dissociable. Specifically, robust changes in intrinsic excitability were observed in the absence of inhibitory plasticity, and shifts in inhibition, but not excitability, appear to be sensitive to developmental stage. Our data establish that while the two forms of homeostatic plasticity can be engaged in parallel, there is a specific order in which they are expressed, with changes in excitability preceding those in inhibition. We propose that changes in intrinsic excitability occur first in order to stabilize network activity while optimizing the preservation of information stored in synaptic strengths by restricting changes that will disrupt the balance of synaptic excitation and inhibition.

2-Amino-5-phosphonovalerate↗

PubMed1987

Maturation of synapses and electrical properties of cells in the cochlear nuclei.

Auditory nerve fibers carry impulses from the cochlea to the cochlear nuclei. There the temporal firing patterns of auditory nerve fibers are preserved by some cells and altered by others. The two factors which govern how firing patterns are shaped are (1) the intrinsic electrical properties of cells that determine the size and time course of voltage changes caused by synaptic currents and (2) the synaptic circuitry between cells. The electrical properties of cells were measured by recording the responses to current injected intracellularly into brain slice preparations. The synaptic responses to electrical shocks of the auditory nerve were used to determine the functional properties of synaptic connections. The three distinct types of electrical properties of cells that can be distinguished electrophysiologically in similar preparations of mature tissue, bushy and stellate cells in the ventral cochlear nucleus [(1984) J. Neurosci. 4, 1577-1588] and cells in the dorsal cochlear nucleus [Hirsch and Oertel (1987) (submitted); Oertel et al. (1987) In: Functions of the Auditory System, Editor: S. Hassler. J. Wiley and Sons (in press)] can be differentiated at least as early as 7 days after birth. Young cells, however, have higher input resistances and lower input capacitances than mature cells, and they cannot sustain high firing rates. Bushy and stellate cells in the ventral cochlear nucleus respond to electrical stimulation of the auditory nerve with both excitatory and inhibitory postsynaptic potentials as early as 4 days after birth. The synaptic potentials occur with longer and more variable latencies than in mature cells and synapses fatigue more easily, however. Cells of the dorsal cochlear nucleus also receive both excitatory and inhibitory synaptic inputs 4 days after birth, upon stimulation of the auditory nerve. No systematic changes were detected in these synaptic responses as a function of age but this may have been because the variability in the shape and timing of synaptic responses was large even in mature tissue.

Action Potentials↗

PubMed1992

Cholinergic interneurons in the feeding system of the pond snail Lymnaea stagnalis. II. N1 interneurons make cholinergic synapses with feeding motoneurons.

The N1 neurons are a population of interneurons active during the protraction phase of the feeding rhythm. All the N1 neurons are coupled by electrical synapses which persist in a high Mg/low Ca saline which blocks chemical synapses. Individual N1 spikes produce discrete electrotonic postsynaptic potentials (PSPS) in other N1 cells, but the coupling is not strong enough to ensure 1:1 firing. Bursts of N1 spikes generate compound PSPS in the feeding motoneurons. The sign (excitation or inhibition) of the N1 input corresponds with the synaptic barrage recorded during the protraction phase. Discrete PSPS are only resolved in a Hi-Di saline. Their variation in latency and number can be explained by variation in electrotonic propagation within the electrically coupled network of N1 cells. The excitatory postsynaptic potentials (ESPS) in the 1 cell are reduced by 0.5 mM antagonists hexamethonium (HMT), atropine (ATR), curare (d-TC) and by methylxylocholine (MeXCh), all of which block the excitatory cholinergic receptor (Elliott et al. (Phil. Trans. R. Soc. Lond. 336, 157-166 (Preceding paper.) (1992)). The 1 cell EPSPS were transiently blocked by phenyltrimethylammonium (PTMA), which is both an agonist and antagonist at the 1 cell excitatory acetylcholine (ACh) receptor (Elliott et al. 1992). The inhibitory postsynaptic potential (IPSP) in the 3 cell is blocked by bath applications of MeXCh and PTMA, which both abolish the response of the 3 cell to ACh (Elliott et. al. 1992). The effects of the cholinergic antagonists on the response of 4 cluster and 5 cells to N1 stimulation matches their response to ACh (Elliott et al. 1992). It is concluded that the population of N1 cells are multiaction, premotor cholinergic interneurons.

Acetylcholine↗

PubMed1997

Intensity and frequency dependence of laryngeal afferent inputs to respiratory hypoglossal motoneurons.

Inspiratory hypoglossal motoneurons (IHMs) mediate contraction of the genioglossus muscle and contribute to the regulation of upper airway patency. Intracellular recordings were obtained from antidromically identified IHMs in anesthetized, vagotomized cats, and IHM responses to electrical activation of superior laryngeal nerve (SLN) afferent fibers at various frequencies and intensities were examined. SLN stimulus frequencies <2 Hz evoked an excitatory-inhibitory postsynaptic potential (EPSP-IPSP) sequence or only an IPSP in most IHMs that did not change in amplitude as the stimulus was maintained. During sustained stimulus frequencies of 5-10 Hz, there was a reduction in the amplitude of SLN-evoked IPSPs with time with variable changes in the EPSP. At stimulus frequencies >25 Hz, the amplitude of EPSPs and IPSPs was reduced over time. At a given stimulus frequency, increasing stimulus intensity enhanced the decay of the SLN-evoked postsynaptic potentials (PSPs). Frequency-dependent attenuation of SLN inputs to IHMs also occurred in newborn kittens. These results suggest that activation of SLN afferents evokes different PSP responses in IHMs depending on the stimulus frequency. At intermediate frequencies, inhibitory inputs are selectively filtered so that excitatory inputs predominate. At higher frequencies there was no discernible SLN-evoked PSP temporally locked to the SLN stimuli. Alterations in SLN-evoked PSPs could play a role in the coordination of genioglossal contraction during respiration, swallowing, and other complex motor acts where laryngeal afferents are activated.

Action Potentials↗

PubMed1991

Distribution of effective synaptic currents underlying recurrent inhibition in cat triceps surae motoneurons.

1. Steady-state recurrent (Renshaw) inhibitory postsynaptic potentials (RIPSPs) were evoked in cat triceps surae motoneurons by stimulating the heteronymous muscle nerve at 100 Hz after dorsal root section. The effective synaptic currents (i.e., the net synaptic current measured at the soma, IN) underlying these inhibitory potentials were measured with a modified voltage-clamp technique. 2. The average value of the effective synaptic currents measured in medial gastrocnemius (MG) motoneurons was 0.4 nA. There was no significant correlation between the IN measured in individual cells and motoneuron input resistance (RN), rheobase (IR), duration of the spike afterhyperpolarization (AHPt1/2), or putative motor-unit type, although the steady-state inhibitory post-synaptic potential (IPSP) amplitudes were correlated with all of these parameters. 3. Steady-state recurrent inhibition was accompanied by a small (3.5%, on average) decrease in the resting input resistance of the motoneurons. The small magnitude of this measured change supports the hypothesis of Burke et al. that the site of synaptic contact between Renshaw cells and motoneurons is somewhat distal to the cell soma. 4. The absence of a differential distribution of the effective synaptic currents generated by Renshaw cells within the MG pool does not support the idea that recurrent inhibition mediates a selective reduction of the firing of small, low-threshold motoneurons by large, high-threshold motoneurons. The small amplitude of the effective synaptic currents we measured suggests that the contribution of recurrent inhibition to the direct modulation of motoneuron firing rate is subtle and that it is perhaps principally involved in the fine control and smooth production of muscle force.

Action Potentials↗

PubMed2002

Intrinsic and synaptic properties of neurons in an avian thalamic nucleus during song learning.

The anterior forebrain pathway (AFP) of the avian song system is a circuit essential for song learning but not for song production. This pathway consists of a loop serially connecting area X in the basal ganglia, the medial portion of the dorsolateral nucleus of thalamus (DLM), and the pallial lateral magnocellular nucleus of the anterior neostriatum (lMAN). The majority of DLM neurons in adult male zebra finches closely resemble mammalian thalamocortical neurons in both their intrinsic properties and the strong GABAergic inhibitory input they receive from the basal ganglia. These observations support the hypothesis that the AFP and the mammalian basal ganglia-thalamocortical pathway use similar information-processing mechanisms during sensorimotor learning. Our goal was to determine whether the cellular properties of DLM neurons are already established in juvenile birds in the sensorimotor phase of song learning when the AFP is essential. Current- and voltage-clamp recording in DLM of juvenile male zebra finches showed that juvenile DLM has two distinct cell types with intrinsic properties largely similar to those of their respective adult counterparts. Immunostaining for glutamic acid decarboxylase (GAD) in juvenile zebra finches revealed that, as in adults, most area X somata are large and strongly GAD+ and that their terminals in DLM form dense GAD+ baskets around somata. GAD immunoreactivity in DLM was depleted by lesions of area X, indicating that a strong GABAergic projection from area X to DLM is already established in juveniles. Some of the DLM neurons exhibited large, spontaneous GABAergic synaptic events. Stimulation of the afferent pathway evoked an inhibitory postsynaptic potential or current that was blocked by the GABA(A) receptor antagonist bicuculline methiodide. The decay of the GABA(A) receptor-mediated currents was slower in juvenile neurons than in adults. In addition, the reversal potential for these currents in juveniles was significantly more depolarized both than that in adults and than the Cl(-) equilibrium potential; yet the reversal potential was still well below the firing threshold and thus inhibitory in the slice preparation. Our findings suggest that the signal-processing role of DLM during sensorimotor learning is generally similar to that in adulthood but that quantitative changes in synaptic transmission accompany the development of stereotyped song.

Action Potentials↗

PubMed1981

Possible mechanisms of enkephalin action on hippocampal CA1 pyramidal neurons.

(1) Intracellular and extracellular recordings were made from CA1 pyramidal neurons in an in vitro rat hippocampal slice preparation, while [D-Ala2, D-Leu5]enkephalin (DADL) was applied by perfusion at a known concentration (1 to 5 X 10-7 M), in a small droplet, or by iontophoresis into the cellular and dendritic layers of the slice. The effects of DADL on synaptic potentials and membrane properties were studied in an effort to determine the mechanisms underlying its epileptogenic action in the hippocampus. (2) DADL increased the size and often the duration of excitatory postsynaptic potentials (EPSPs) generated on either the apical or basal dendrites; this resulted in an increased discharge probability for a constant orthodromic stimulus. Extracellular field potential recordings showed a larger population spike for a given size field EPSP. These effects of DADL could be reversed substantially by perfusion with naloxone (1 to 5 X 10-7 M) and appeared qualitatively different from the epileptiform actions of penicillin. (3) DADL did not appear to increase the intrinsic excitability of the soma membrane, since membrane potential, input resistance, spike threshold, and antidromic field potentials all were unchanged. In addition, the shape of the membrane charging curve during hyperpolarizing current injection was not changed noticeably by DADL. (4) At the concentrations tested, DADL did not attenuate recurrent inhibition in the CA1 region, as evaluated by comparing in the absence and presence of DADL: (a) antidromically evoked recurrent inhibitory postsynaptic potentials (IPSPs) and their dependence of membrane potential, (b) the reduction of a synaptically driven population spike by a prior antidromic volley, (c) iontophoretic GABA (gamma-aminobutyric acid) responses. Similarly, IPSPs evoked by orthodromic stimulation appeared either unaffected or occasionally enhanced by DADL. (5) By iontophoretic mapping, it was shown that the DADL-sensitive sites are limited to stratum oriens and stratum pyramidale. Local application of DADL into stratum radiatum was relatively ineffective in enhancing the efficacy of synapses located in this region. (6) The dendritic input-output relationship between the presynaptic fiber volley and the field EPSP was not changed by DADL. This finding and the results of the iontophoretic mapping experiments suggest that increased excitatory transmitter release was not involved. (7) The data are consistent with the proposal that DADL selectively attenuates a dendritic IPSP which is virtually invisible to the soma, although the possibility cannot be ruled out that DADL may, in addition, act to enhance the responsiveness of pyramidal dendritic membrane to excitatory synaptic activation.

Animals↗

PubMed2004

Properties of horizontal axo-axonic cells in stratum oriens of the hippocampal CA1 area of rats in vitro.

Local-circuit gamma-aminobutyric acid (GABA)ergic interneurons constitute a diverse population of cells, which remain poorly defined into functionally distinct subclasses. Traditionally, dendritic and axonal arbors have been used to describe cell classes. In the present report, we characterize a set of hippocampal interneurons, horizontal axo-axonic cells, located in stratum oriens. They displayed the pattern of axonal arborization characteristic of axo-axonic cells with radially aligned rows of boutons making synapses exclusively on axon initial segments of pyramidal cells, as shown by electron microscopy. However, in contrast to previously described axo-axonic cells, which have radial dendrites spanning all layers, the dendrites of the horizontal axo-axonic cells were restricted to stratum oriens and ran parallel with the layers for several hundred micrometers. Single action potentials elicited by depolarizing current steps in these cells were often followed by a fast- and medium-duration afterhyperpolarization, distinguishing them from fast-spiking interneurons. In two out of four cells, trains of action potentials showed prominent early spike frequency adaptation and a characteristic "accommodative hump." Excitatory postsynaptic potentials (EPSPs) could be evoked by stimuli delivered to stratum oriens. Paired recordings unequivocally confirmed direct synaptic inputs from CA1 pyramidal cells. The kinetics of the EPSPs were fast (rise time 1.7 +/- 0.6 ms, mean +/- SD, n = 3; decay time constant 19.3 +/- 2.4 ms). They showed paired-pulse depression with inter-stimulus intervals of 10-50 ms. One pair showed a reciprocal connection establishing a direct feedback loop. The axo-axonic cell-evoked inhibitory postsynaptic potentials (IPSPs) were reliable (failure rate approximately 10%). Our data show that the laminar distribution of the dendrites of axo-axonic cells can vary, suggesting distinct synaptic inputs. However, this remains to be shown directly, and we cannot exclude the possibility that all axo-axonic cells may gather similar synaptic input, leaving them as one distinct class of interneuron.

Action Potentials↗

PubMed2004

Alpha 2-adrenergic receptor-mediated presynaptic inhibition of GABAergic IPSPs in rat histaminergic neurons.

Nuclei of the brainstem involved in behavioral state control are mutually interconnected. Histaminergic neurons of the posterior hypothalamus receive inputs from brainstem noradrenergic cell groups as well as from the locus coeruleus. The role of adrenergic inputs in histaminergic function is unclear. We examined the actions of adrenergic agonists on histaminergic neurons of the tuberomamillary nucleus (TM) using electrophysiological methods in a brain slice preparation. Evoked GABAergic inhibitory postsynaptic potentials (IPSPs) in histaminergic neurons were reduced in amplitude following the application of norepinephrine (NE) (2-20 microM) or clonidine (10 microM) but were not affected by isoproterenol (10 microM). Norepinephrine application caused no changes in membrane properties of TM neurons. Responses to exogenously applied GABA were unaffected by adrenergic agonists. Clonidine reduced the frequency of spontaneous IPSPs, an action that was blocked by yohimbine. Norepinephrine did not alter the amplitude distribution of bicuculline-sensitive miniature inhibitory postsynaptic currents (mIPSCs). Thus, GABA release onto TM neurons is modulated presynaptically by adrenergic alpha(2)-receptors. Inputs from noradrenergic neurons of the brainstem will reduce the inhibitory actions of GABAergic inputs resulting in disinhibition of histaminergic neurons.

Adrenergic alpha-2 Receptor Agonists↗

PubMed1986

Postsynaptic potentials of bulbar respiratory neurons of the turtle.

Membrane potentials of bulbar respiratory neurons of Pseudemys scripta were recorded from unanesthetized, decerebrate animals. Efferent neurograms were recorded from the serratus magnus (inspiratory) and pectoralis (expiratory) nerves. The animals were paralyzed and ventilated by a servo-respirator driven by the pectoralis neurogram to mimic the spontaneous turtle respiratory cycle. Neurons, comparable to mammalian medullary respiratory neurons, were observed, namely: augmenting inspiratory, augmenting expiratory and postinspiratory neurons. In addition, two types of phase-spanning neurons, postinspiratory-expiratory and expiratory-inspiratory, were encountered. The pattern of appearance of inhibitory postsynaptic potentials generally resembled that observed in the cat. The neurons observed correspond to the activity pattern of known respiratory muscles of the turtle. The results suggest that the three phase respiratory cycle of the turtle corresponds to the ternary neural cycle postulated for mammals.

Action Potentials↗

PubMed1990

Slow intrinsic spikes recorded in vivo in rat CA1-CA3 hippocampal pyramidal neurons.

The membrane potential of 45 CA1-CA3 hippocampal pyramidal cells was recorded in curarized and urethanized rats. Two slow spike types were observed together with the usual Na+ type action potential. Slow spikes were termed HTS and LTS because they were essentially identical to the high and low threshold Ca2+ spikes observed in vitro and probably represent the same kinds of activities. LTS and HTS (22 and 29 cells, respectively) were triggered at potentials greater than or equal to 65 mV and less than or equal to 55 mV, had mean durations of 23.7 and 90.4 ms and mean amplitudes of 11.5 and 39.3 mV, respectively, and fired an overriding burst of the action potentials. LTS and HTS were sometimes present in the same neuron (n = 16). Depolarizing pulses triggered rhythmic HTS at rates that increased with depolarization up to a 5 impulses/s maximum. The same limit was found with imposed membrane potential sine currents at frequencies within the theta rhythm range. With spontaneous or imposed hyperpolarizations LTS were evoked by depolarizing, at the break of hyperpolarizing pulses, or spontaneously. They were also evoked at the depolarizing, or recovery, slopes of inhibitory postsynaptic potentials. HTS and LTS supply pyramidal neurons with different firing patterns which enhance the system's integrative possibilities. Evidence is provided that theta is not exclusively generated by network properties, since rhythmic HTS may participate.

Action Potentials↗