The use of tricalcium phosphate for the adsorption and concentration of ervsipelas bacterin.
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Various water-borne bacteria can cause significant illness. Illness most often results from ingestion of contaminated water or seafood, with gastrointestinal entry of pathogens or their byproducts. The skin and soft tissues are also common entry points, either through trauma in or near the water, or by seawater contamination of an existing wound or break in the skin. Clinically significant water-borne illnesses are still rare. However, because humans are increasingly exposed to contaminated water sources through recreation or habitat, the rate of water-borne illnesses can be expected to increase. In patients who present with gastrointestinal illness or infections that do not respond to conventional therapy, water-borne pathogens should be considered in the differential diagnosis. A complete history, with attention to seafood ingestion and exposure to marine or freshwater life-forms, in conjunction with a high index of suspicion (especially in immunocompromised patients), will increase diagnostic accuracy in patients with water-borne illnesses.
Different serological methods were used in determining the valence of red murrain immune sera. By using a grow test it is possible to accomplish an immunological differentiation of the immune sera. The used ELISA was tested in comparison to the conventional mouse protective test (PRIGGE, 1950) with regard to its valuation of immune sera. Both procedures have been tested on a possible correlation. In almost all cases a correlation between high titres in ELISA and a mouse protective value over 100 I.E. could be proven. The statistical processing of the test results proved an assumed correlation of both procedures. But it is necessary to carry out further research in order to be able to recommend this procedure as an alternative for the mouse protection test.
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The Km-values of neuraminidases from different pathogenic and apathogenic microorganisms have been determined on low and high molecular substrates. The substrate specificity and the affinity to the different types of substrates in relation to the pathogenicity of the microorganisms are discussed.
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During 1985 four external quality assessment tests for clinical microbiology were performed, each consisting of four simulated clinical specimens. The results are reported, evaluated and some problem areas discussed.
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Tested was the simultaneous vaccination of pigs against swine fever, swine erysipelas, and Aujeszky's disease. Parallel to this experiment a second group of test pigs were treated with each of the three vaccines at twenty-day intervals. The effect of vaccination was assessed through clinical observations with the test groups and by challenging part of the animals with virulent strains of the swine fever and erysipelas agents. It was found that the successive application of the three vaccines at 20-day intervals (at the age of 50, 70, and 90 days) could induce immunity that lasted for a longer period than the immunity resulting from the simultaneous treatment at the age of 90 days. In order to protect the pigs for sure up to the end of the fattening period a revaccination against swine fever with the simultaneous use of the vaccine against swine erysipelas in both cases was necessary.
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A peracute severe outbreak of erysipelas in chukars (Alectoris chukar) caused 55% mortality. Pheasants and quails on the same premises were not affected. Possible sources of infection and pathogenesis are discussed.