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PubMedDate not supplied

[Native and artificial sub-forms of cytoplasmic aspartate transaminase from swine heart].

Multiple forms of aspartate transaminase from cytosol of pig heart (alpha, beta and psi) were incubated in 0.05 M acetate buffer, pH 5.0 at 4 degrees within 5 and 8 months. Gradual accumulation of denaturated forms was observed on incubation of alpha- and beta-forms; these forms possessed higher mobility in polyacrylamide gel disc electrophoresis and decreased enzymatic activity as compared with the native forms. Multiple forms, produced during ageing of alpha-form, were separated by chromatography on CM-cellulose. Chromatographic resolution of the forms, their activity and spectral properties suggest that they are not identical with the native isoenzymes found in the cell.

Animals↗
PubMed1999

Genetic homogeneity, high-resolution mapping, and mutation analysis of the urofacial (Ochoa) syndrome and exclusion of the glutamate oxaloacetate transaminase gene (GOT1) in the critical region as the disease gene.

The urofacial (Ochoa) syndrome (UFS) is a rare autosomal recessive disorder characterized by abnormal facial expression and urinary abnormalities. Previously, we mapped the gene to a genomic interval of approximately 1 cM on chromosome region 10q23-24, using families from Columbia. Here we demonstrate genetic homogeneity of the syndrome through homozygosity mapping in American patients with Irish heritage. We established a physical map and identified novel polymorphic markers in the UFS critical region. Haplotype analysis using the new markers mapped the UFS gene within one YAC clone of 1,410 kb. We also determined the precise location of the gene encoding for glutamate oxaloacetate transaminase (GOT1) within the new UFS critical region and determined its genomic structure. However, mutation analysis excluded GOT1 as a candidate for the UFS gene.

Abnormalities, Multiple↗
PubMed1975

[Glutamate-oxalacetate-transaminase in irradiated and nonirradiated chick embryos].

The enzymatic activity of glutamate-oxalacetate transaminase (GOT) in 16 days old chick embryos was investigated in 10 minute-intervals following X-ray irradiation with 1000 R. Initially, in the tissue homogenates of the embryos and in the blood serum a decline of enzyme activity was observed, which continued for 30 minutes after irradiation. Subsequently - during a second phase - the enzymatic action was increased far beyond the norm. On the contrary, in the tissues of the allantois no significant change of the GOT-concentration was seen following irradiation.

Allantois↗
PubMed1975

[Behavior of glutamic-oxalacetic transaminase in the serum of a group of cholecystopathic patients].

The activity of glutamic-oxalacetic transaminase was studied in the serum of patients suffering from cholelithiasis. Patients were divided into chronic sufferers with jaundice complication and those without jaundice; a similar subdivision was made for patients with acute cholecystitis. The data showed an increase in the frequency of the alteration in enzyme activity in patients suffering from acute forms, probably as a result of the greater incidence of jaundice than is the case with chronic cholelithiasis.

Acute Disease↗
PubMed1975

Human deficiencies of vitamin B6. I. Studies on parameters of the assay of the glutamic oxaloacetic transaminase by the CAS principle.

Human deficiencies of vitamin B6 may be detected and accurately measured by a dual enzymic assay which is based on the CAS principle, i.e., the unsaturation and saturation of the coenzyme (pyridoxal 5'-phosphate) of a Coenzyme - Apoenzyme System. Glutamic oxaloacetic transaminase (GOT) is excellent for this assay. Two control groups (24 athletes and 25 students) showed deficiencies of GOT activity of 11+/-6% and 12+/-7%, respectively, which are negligible and which may be compared with analogous data from disease states. Contrary to a previous report, GOT activities must be determined within one day of preparation of the enzyme for meaningful assays. Frozen storage of erythrocyte hemolysates up to 21 days showed increasing apparent dissociation of pyridoxal 5'-phosphate from the coenzyme - apoenzyme system corresponding to increasing false deficiency levels. Dilution of the hemolysates and frozen storage augmented the apparent dissociation and false levels. This dual GOT assay is effective to detect and measure human deficiencies of vitamin B6.

Apoenzymes↗
PubMedDate not supplied

[The specific features of lipid metabolism and changes in the plasma activity of transaminases in patients with sepsis].

A parallel study of changes in the activity of transaminases and the parameters of lipid metabolism was conducted in patients with sepsis or septic shock, receiving renal replacement therapy. The multiple baseline increase in the activity of gamma-glutamyltranspeptidase was observed in about 50% of the patients and the elevated level of triglycerides and very low density-lipoprotein cholesterol in all the examinees. In case of the baseline multiple increased activity of gamma-glutamyltranspeptidase as compared with the normal physiological values, the probability of a good clinical outcome was some 67%, in the survivors, the activity of the enzyme significantly increasing during therapy. In the absence of the baseline multiple increased activity of gamma-glutamyltranspeptidase, there was a good clinical outcome provided that there were positive changes in triglycerides and very low-density lipoprotein cholesterol during therapy and its probability was about 33%. It is concluded that the activity of gamma-glutamyltranspeptidase and the concentration of triglycerides and very low-density lipoprotein cholesterol may be used to evaluate the efficiency of treatment and as predictors of the outcome of treatment in patients with sepsis and septic shock.

Adult↗
PubMed1989

Comparative value of glutamic oxaloacetic transaminase and lactic dehydrogenase enzyme pattern in serum and cerebrospinal fluid in acute cerebrovascular accidents.

A study of glutamic oxaloacetic transaminase (GOT) and lactic dehydrogenase (LDH) enzymes in serum and cerebrospinal fluid (CSF) was carried out on 30 cases of acute cerebrovascular accident (CVA) to determine their role in precise diagnosis and prognosis of the cases. The CSF GOT and LDH activities were well correlated with the severity, mortality and peak and proper timing in relation to infarct, same is about serum GOT. A characteristic rise of GOT and LDH was observed in cerebral haemorrhage especially in fatal cases. However, in cerebral thrombosis and embolism specially in non-fatal cases, the enzyme change was not so marked. GOT and LDH enzyme pattern in CSF and serum may, therefore, serve as an early, relatively easy and widely available tool for differential diagnosis and prognosis of cases of acute CVA.

Aspartate Aminotransferases↗
PubMedDate not supplied

Glutamic-oxaloacetic transaminase isozymes in human seminal plasma and sperm extracts.

Leakage of glutamic-oxaloacetic transaminase (GOT) can be used as an indicator of cryo-damage for mammalian spermatozoa. However, two GOT isozymes exist: mitochondrial (m-GOT) and cytosolic or soluble (s-GOT), with acid and alkaline pH optima, respectively. The levels of these GOT isozymes were determined in samples of cell-free human seminal plasma and sperm extracts over a pH range of 3.8 to 9.6. Optimum GOT activities were found at pH 5.2 using cacodylate-HCl buffer (m-GOT) and pH 8.4 using barbital buffer (s-GOT). Differential inhibition studies with adipate (which inhibits s-GOT) were misleading because of the presence of another NADH-linked enzyme able to use adipate as a substrate. Only relatively low levels of m-GOT could be extracted from human spermatozoa (21.4 +/- 31.5 [SD] mU/10(8) cells vs. 202.3 +/- 49.4 mU/mL seminal plasma; n = 20). Consequently, future studies on GOT leakage should be carried out at the alkaline pH optimum, and will therefore measure s-GOT, making this a marker for plasma membrane integrity rather than a measure of mitochondrial damage.

Aspartate Aminotransferases↗
PubMed1985

Effect of metal ions on the aspartate transaminase (E.C.2.6.1.1.) activity in tobacco tissue culture.

Aspartate transaminase enzyme was prepared from tobacco tissue cultures. Effect of 13 different metal ions on the enzyme activity was preliminarily studied. The enzyme activity was inhibited by five ions, namely Cd2+, Hg2+, Zn2+, Cu2+, and Ag+. None of the ions investigated enhanced the activity. Fe2+ caused an apparent activity increase in the reaction mixture. Pyridoxal-phosphate enhanced this effect of the Fe2+.

Aspartate Aminotransferases↗
PubMed1985

Biochemical changes of the synovial liquid of corpses with regard to the cause of death. 2: Alkaline phosphatase, lactic acid dehydrogenase (LDH), and glutamic oxalacetic transaminase (GOT).

We studied the activity of various enzymes in the synovial liquid of 100 corpses with regard to the cause of death finding that the alkaline phospatase and glutamic oxalacetic transaminase (GOT) are increased in cranioencephalic trauma, possibly as a result of the important cellular lysis which goes with them; and lactic acid dehydrogenase (LDH) is increased in the pulmonary processes, almost certainly with relation to the great quantity of this enzyme in the lung.

Adolescent↗
PubMedDate not supplied

[Spectral properties of aspartate transaminase from chicken heart cytosol].

For the first time an interaction between aspartate transaminase (EC 2.6.1.1.) from chicken heart cytosol and the substrates and their analogues has been investigated by means of circular dichroism and absorption spectra (at pH 5,0-8,0 range). The asymmetry factor of the native enzyme and the enzymes--substrate intermediates was found. The results obtained were explained in terms of changes of the enzyme's active site conformation.

Animals↗
PubMed1985

Time course of serum cardiac enzymes after intracoronary thrombolytic therapy. Creatine kinase, creatine kinase MB isozyme, lactate dehydrogenase, and serum glutamic-oxaloacetic transaminase.

We analyzed the time course of serum creatine kinase (CK), the CK MB isozyme, lactate dehydrogenase (LDH), and serum glutamic-oxaloacetic transaminase (SGOT) activity and calculated rates of increase and decline for CK in 24 consecutive patients with acute myocardial infarction (AMI) who received intracoronary thrombolytic therapy. In 19 patients with successfully reperfused infarcts, peak CK activity occurred at 14.1 +/- 1.1 hours after onset of symptoms, the maximal rate of CK rise was 595 +/- 102 IU/L/hr, and the fractional disappearance rate (Kd) was (86 +/- 6) X 10(-5)/min. The peak CK MB activity occurred at 12.9 +/- 0.8 hours and the MB Kd was (223 +/- 39) X 10(-5)/min. In five patients in the nonreperfused group the peak CK (24.9 +/- 4.5 hours) and CK MB (22.7 +/- 3.3 hours) activity occurred later, the maximal rate of CK rise (281 +/- 37 IU/L/hr) was less, and the CK Kd [(68 +/- 5) X 10(-5)/min] and MB Kd [(116 +/- 28) X 10(-5)/min] were lower. The peak CK, CK MB, cumulative CK release, and area under the curve were not different. Except for a shortened time to peak SGOT in the reperfused (17.1 +/- 1.3 hours) compared with the nonreperfused (29.1 +/- 5.6 hours) groups, the time course of LDH and SGOT were not different. Thus, the initial serum CK kinetics and time to peak SGOT may be useful in assessing the reperfusion status in patients with AMI receiving thrombolytic therapy without coronary angiography or in those who may have spontaneous recanalization.

Adult↗
PubMed1966

A comparison of serum creatine phosphokinase and serum glutamic oxalacetic transaminase in skeletal muscle necrosis.

Rabbits were poisoned by the intraperitoneal injection of plasmocid dihydroiodide at a dosage of 80 mg. per kg. body weight. Animals were euthanatized 1, 2, 5, 7, 9, 12, 24 and 48 hours after plasmocid injection and serum and diaphragmatic muscle collected. The serum was examined for its serum glutamic oxalacetic transaminase (SGOT) and creatine phosphokinase (CPK) concentrations. The diaphragm was sectioned and examined for the progression of morphologic alterations. It was found that SGOT and CPK concentrations were similar in their time and duration of elevation and that the serum CPK elevations closely paralleled the occurrence and degree of morphologic muscle degeneration. It appears that CPK is the most specific clinical biochemical test for the detection of muscle necrosis available at this time.

Animals↗
PubMed1979

Stereochemistry of holoaspartate transaminase after modification of the active site Lys-258.

Proton incorporation at position C4 of the substrate-coenzyme Schiff base of aspartate transaminase is a stereospecific process. After carbamylation of the active site Lys-258, the stereospecificity of the reaction in 2H2O is retained. By a correlation method, it is shown that addition occurs from the si side of the complex and the pyridoxamine phosphate produced is deuterated at position pro-S of the pyridoxamine methylene group. These results constitute a demonstration for the stereochemstry of a half-transamination process of the phosphorylated coenzyme under single turnover conditions. They also illustrate that free Lys-258 is not required to maintain stereospecificity and cast doubts on the implication of this residue as a participant in C4 proton addition during catalysis by the native form of this mammalian enzyme.

Animals↗
PubMed1967

Serum enzymes in patients with carcinoma of lung: lactic-acid dehydrogenase, phosphohexose isomerase, alkaline phosphatase and glutamic oxaloacetic transaminase.

Serial determinations of serum lactic-acid dehydrogenase (LDH), phosphohexose isomerase (PHI), alkaline phosphatase (AP), and glutamic oxaloacetic transaminase (GOT) were carried out in 30 patients with primary and four with secondary neoplasms of the lung. Enzyme values were correlated with the stage of illness, with tumour histology, with chemotherapy and with the extent and site of metastases as determined at autopsy. The activity of the enzymes LDH and PHI was most frequently elevated; their values correlated closely. AP and GOT tended to become elevated only shortly before death. Although, in general, enzyme activity increased with tumour extension and often in relation to chemotherapy, assays provided little assistance in early diagnosis or prognosis except that, in most instances, elevated values of any of the four enzymes indicated the presence of metastases.

Alkaline Phosphatase↗
PubMedDate not supplied

Elevated transaminases and hepatomegaly in an obese diabetic.

A 61-year-old obese female presented with hypothyroidism, insulin-independent diabetes mellitus, and asymptomatic hepatomegaly. The diagnostic evaluation of hepatomegaly with elevated transaminase is discussed. Liver biopsy findings are discussed. Plans for therapy are presented and evaluated.

Aspartate Aminotransferases↗
PubMed1980

Activity of red cell glutamic oxaloacetic transaminase in children suffering from disturbed motion.

Vitamin B6 status was investigated in 37 children suffering from disturbed motion and in 25 healthy children by mean of determination of activity of red cell glutamic oxaloacetic transaminase. 7 out of 37 patients were treated with antiepileptic drugs. Patients had a biochemical vitamin B6 deficiency when compared with controls. There was no significant difference in the degree of the vitamin B6 deficiency between patients with and without antiepileptic treatment. The biochemical vitamin B6 deficiency seems to be due to a decreased nutrient intake in patients with disturbed motion.

Adolescent↗