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A dynamical Bragg equation for high-order Laue zone reflections

A dynamically corrected Bragg equation for high-order Laue zone (HOLZ) reflections is derived directly from the Bloch wave formalism instead of the geometric argument used to deduce the kinematical Bragg condition. It differs from the kinematical Bragg equation by replacing the plane wave vector in the kinematical equation with the Bloch wave vectors. This dynamical equation reduces to the kinematical equation when the crystal potential is zero. It also demonstrates the occurrence of dynamical shifts for the HOLZ reflections but their absence for the zero-order Laue zone (ZOLZ) reflections in the symmetrical Laue case.

Journal Article↗

Cerebral vasomotion: a 0.1-Hz oscillation in reflected light imaging of neural activity.

Imaging of scattered and reflected light from the surface of neural structures can reveal the functional architecture within large populations of neurons. These techniques exploit, as one of the principal signal sources, reflectance changes produced by local variation in blood volume and oxygen saturation related to neural activity. We found that a major source of variability in the captured light signal is a pervasive low-frequency (0.1-Hz) oscillation which apparently results from regional cerebral blood flow. This signal is present in brain parenchyma as well as the microvasculature and exhibits many characteristics of the low-frequency "vasomotion" signals observed in peripheral microcirculation. Concurrent measurements in brain with a laser Doppler flow meter contained an almost identical low-frequency signal. The presence of the 0.1-Hz oscillation in the cerebral microcirculation could underlie a portion of the previously described characteristics reported in reflected-light imaging studies. The prevalence of the oscillatory phenomena in the brain raises substantial temporal sampling issues for optical imaging and for other visualization techniques which depend on changes in regional cerebral blood dynamics, such as functional magnetic resonance imaging.

Animals↗

Influence of PaO2 on cerebral macro- and microcirculation as observed by light reflection: time course of changes.

A silicon intensified target camera was used to study cerebral cortical vessels of the cat through a skull window implant, and red cell content changes were measured by light reflectance. Red cell content changes were observed in cerebral arterioles, capillaries, and venules when the PaO2 was decreased by lowering the PiO2. The time course of change in the diameter of the arterioles and venules was measured by selecting a cross section of the vessel plus some surrounding tissue. From the averaged cross-sectional reflectance signal, the change in vessel diameter was followed as a function of time following the PiO2 change. All vessels of greater than 10 microns were observed in focus. Substantial areas where no vessels could be discriminated would contain only capillaries, and changes in light reflectance from such areas would indicate changes in capillary red cell content. The time course of these changes following a step decrease in PiO2 was recorded. Results show that the sequence of red cell content increase in cerebral microcirculation during hypoxia is capillary before venule and arteriole. The times of initial red cell content increase are 37.9 +/- 7 s, 59.7 +/- 7.9 s, and 60.8 +/- 9.1 s, respectively. These results suggest an increase in the capillary bed red cell content as the initial response to hypoxia, but venules and arterioles change only on longer exposure to hypoxia. The sequence of the increase in red cell content suggests the capillaries rather than the arterioles are the vessels which respond to the oxygen autoregulation signal.

Animals↗

Spectrophotometric investigation of pulsatile blood flow for transcutaneous reflectance oximetry.

A new method to estimate arterial oxygen saturation is suggested based on the presence of the plethysmographic pulse. A prototype skin reflectance oximeter system utilizing a miniature solid state optical transducer has been constructed. Preliminary data collected from a group of volunteers indicated good quantitative correlation between skin reflectance and arterial oxygen saturation as determined by an earlobe oximeter. We are now investigating the dependency of the measurements on various physiological conditions and skin characteristics in order to determine appropriate calibration procedures. This study has demonstrated for the first time that arterial hemoglobin oxygen saturation can be measured quantitatively using transcutaneous reflectance techniques. However, more extensive work is needed to further develop this technique into a viable clinical tool.

Humans↗

Self-assessment of angles of strabismus with photographic Purkinje I and IV reflection pattern evaluation.

BACKGROUND: Accurate assessment of the angle of strabismus, e.g. of variable angles of strabismus, is crucial in preoperative patient management and is usually performed in a clinical environment. Objective assessment by patients themselves, under everyday conditions, could contribute to a better preoperative work-up. A new objective evaluation procedure for the measurement of manifest angles of strabismus for near and distance fixation by the patient himself is presented. METHODS: To account for the modified experimental setup used for the self-assessment, an amended computation procedure of Purkinje reflection pattern evaluation was developed. For measurement, patients and controls placed their head on a head/chin rest and fixated at 33 cm or 4 m distance in primary position. A reflex camera and three photo flash units were positioned on a special frame underneath the visual axis and in front of the subject so that both eyes could be photographed simultaneously. The camera's remote shutter control was released together with the photo flash units by the properly fixating subject. The angles of strabismus were obtained from the series of pictures through later evaluation of the Purkinje I and IV reflection patterns recorded in the photographs of the eyes. RESULTS: Measurements of the ocular alignment in two control groups and in a group of strabismic subjects showed satisfactory accuracy of the "self-assessment" method compared to "standard" Purkinje reflection pattern evaluation and orthoptic measurements of the angle of strabismus. CONCLUSION: The modified "self-assessment" method can be used for the objective recording of angles of strabismus as needed in the preoperative work-up of patients with variable angles of strabismus, over prolonged periods of time, and outside a clinical setting.

Adolescent↗

A method for computing spectral reflectance.

Psychophysical experiments show that the perceived colour of an object is relatively independent of the spectrum of the incident illumination and mainly depends on the surface spectral reflectance. We first demonstrate a possible solution to this undetermined problem for a Mondrian world of flat rectangular patches. We expand the illumination and surface reflectances in terms of a finite number of basis functions. We assume that the number of colour receptors is greater than the number of basis functions. This yields a set of nonlinear equations for each colour patch. Number counting arguments show that, given a sufficient number of surface patches with the same illumination, there are enough equations to determine the surface reflectances up to an overall scaling factor. This theory is similar to previous and independent work by Maloney and Wandell (Maloney 1985). We demonstrate a simple method of solving these non-linear equations. We generalize to situations where the illumination varies in space and the objects are three dimensional shapes. To do this we define a method for detecting material changes, a colour edge detector, and illustrate a way of detecting the colour of a material at its boundaries and propagating it inwards.

Color Perception↗

Improved detection and quantification of the (immuno) peroxidase product using reflection contrast microscopy.

Reflection contrast microscopy (RCM) is a sensitive tool to detect minor amounts of precipitated diaminobenzidine (DABox) in immunoperoxidase stained specimens. One of the main issues in immunocytochemistry is the ongoing need for more sensitive and quantitative techniques. Therefore we applied RCM, using a new simple model system, to methods previously described for increased sensitivity in immunocytochemistry with bright field microscopy. Addition of imidazole was found the most sensitive method and addition of Nickel and Cobalt ions gave the most enhanced colour intensity. Variation of the enzyme reaction parameters yielded a continuous increase in reflection with time. This was then discussed in view of other model studies of peroxidase kinetics. A quantitative relationship between the amount of peroxidase and the reflection of DABox was observed, indicating that quantitative immunoperoxidase studies with RCM are feasible. In situ hybridization (ISH) was then used as a useful biological model for RCM to test the optimal conditions for DAB staining found in the model system (high concentrations of DAB and peroxidase and 2 h incubation time). There was no background staining in the model system, also after prolonged incubation time. The ISH experiments showed that the contrast (ratio) between specific signal and chromosome background did not increase in time, whereas only the use of high avPO concentrations yielded the highest contrast.

3,3'-Diaminobenzidine↗

A fiberoptic reflection oximeter.

A catheter tip oximeter is described consisting of a cardiac catheter containing optical fibers, and incandescent light source, a light detection unit and a processing unit. Half of the optical fibers guide the light to the blood at the tip of the catheter, the other half the backscattered (reflected) light to the detection unit. The detection unit contains a dichroic mirror, transmitting most of the light with lambda less than 800 nm and reflecting most of the light with lambda greater than 900 nm, thus splitting the light into two beams. These pass through interference filters with nominal wavelengths of 640 and 920 nm respectively, and are focused on silicium barrier layer photocells. The photocell signals are amplified and fed into a divider giving the ratio of measuring (R640) and compensating (R920) photocell output. The relationship between log R640/R920 and oxygen saturation is represented by a slightly curved line. The relation may be linearized by subtracting a constant voltage from the divided output before taking the logarithm. The slope of the calibration line is dependent on the total haemoglobin concentration. Nonetheless an average calibration line can be used between 70 and 100% oxygen saturation. For 78 measurements of pig blood samples in this range (haemoglobin concentration between 96 and 161 g.1(-1)), the standard deviation of the difference between the fiberoptic oximeter and a Radiometer OSM1 oxygen saturation meter was 1.9% saturation, for 152 samples over the entire saturation range the standard deviation of the difference was 3.1% saturation. The influence of the flow velocity of blood on the light reflection depends on wavelength as well as on oxygen saturation. Therefore, complete compensation for the flow effect is not possible by simple means.

Animals↗

Pulse wave reflection at the collapsed segment of an artery in Riva-Rocci's method.

The contours of pressure and flow of a pulse wave arriving at the entrance of a collapsed segment of an elastic tube can be described by application of the basic laws of reflection. The collapsed segment acts like a valve. It is opened by the pressure of the pulse wave when the pressure within the tube becomes higher than the "collapse pressure", i.e. the pressure exerted on the segment from outside. This opening of the collapsed segment suddenly changes the sign of the pulse wave reflection from positive to negative; thus a typical contour change of the pulse proximal to the segment is observed. The most obvious effect is a reduction of the pulse pressure. The extent of this reduction is a function of the collapse pressure. A typical example of this phenomenon can be observed in an artery proximal to the segment which is collapsed due to pressure exerted by an inflated Riva-Rocci cuff. The effect is explained by the assumption that the sign of reflection changes when the pressure of the pulse wave crosses the level of the cuff pressure. This explanation is supported by the results of model experiments.

Blood Pressure Determination↗

Osmotic work across inner medullary collecting duct accomplished by difference in reflection coefficients for urea and NaCl.

To demonstrate that osmotic work can be accomplished across the inner medullary collecting duct (IMCD) by the difference in reflection coefficients for urea and NaCl, phenomenological coefficients for urea and NaCl transport were determined in isolated segments of the hamster IMCD perfused in vitro. Arginine vasopressin at 100 microU/ml increased urea permeability from 11.5 +/- 2.9 to 31.7 +/- 4.2 x 10(-7) cm2 s-1 in the middle IMCD but not in the upper IMCD. Urea transport in the middle IMCD consisted of two components, transport with saturable kinetics and simple passive diffusion. Permeability to Na+ was very low (2 x 10(-7) cm2 s-1). Reflection coefficients as measured by the equiosmolality method, with raffinose being a reference solute, were 0.87 +/- 0.05 and 0.71 +/- 0.04 for urea and 1.03 +/- 0.07 and 0.91 +/- 0.04 for NaCl in the upper and the middle IMCD, respectively. Reflection coefficient for urea in the middle IMCD was 0.68 when determined by the zero volume flux method. When the middle IMCD was perfused with bicarbonate Krebs-Ringer (BKR) solution containing 200 mmol/l urea, the replacement of urea in the bathing fluid with equisomolal NaCl caused large volume flux (3.81 +/- 0.45 nl mm-1 min-1) associated with dilatation of intercellular space. The existence of vasopressin in the bath was essential for this phenomenon. This effect was inhibited by 5 x 10(-4) M phloretin in the bath, suggesting that the vasoressin-stimulated urea transport is responsible for this phenomenon. From these observations, we conclude that transport parameters of the middle IMCD are appropriate for accomplishment of osmotic work across this segment in the absence of physicochemical osmotic gradients.

Animals↗

The tunnel effect, Gibson's perception theory, and reflective seeing.

Subjects can have continuous visual experience of an object's movement across a display though the movement's middle phase takes place behind an opaque screen. The present article considers explanatory issues pertaining to this so-called, tunnel effect, with special reference to Gibson's perception theory and the visual activity that I have been calling reflective seeing. Among the issues discussed are the following. (a) In the tunnel experiments, I suggest, there occur both persisting perception, as Michotte held, and persistence perception, as Gibson held. The subjects pick up stimulus information that allows visually experiencing the object's going out of sight at one edge of the screen and coming back into sight at another edge of the screen; the subjects have visual experience of the continued existence and movement of the object while it is out of sight. Moreover, persistence of perceptual experience is involved: when the object goes out of sight, the subjects' visual experience of its movement goes on. (b) I also argue that the tunnel effect is a phenomenon of both straightforward and reflective seeing. Adopting a phenomenal attitude, as one does when reporting one's perceptual experience, one still sees movement taking place on the other side of the screen, as one does in straightforward seeing. However, whereas straightforward seeing does not give inner awareness of visual experience, the subjects in the tunnel experiments report visually experiencing the object's movement while also visually experiencing the opaque screen in front of it as opaque. I argue that these reports, and those about the object's going out of and coming back into sight, must be based on the kind of visual experience that is part and product of reflective seeing.

Attention↗

Visualization of silver-enhanced reaction products from protein-and immuno-colloidal gold probes by laser scanning confocal microscopy in reflection mode.

We have employed a laser scanning confocal microscope in reflection mode to directly and indirectly visualize sites of deposition of silver-enhanced reaction products from colloidal gold probes. A direct approach was used for the localization of alpha-fetoprotein receptors in human myoblasts by incubating primary cultures with an alpha-fetoprotein-gold conjugate. For an indirect approach, cultured CEM cells, derived from a human T-lymphoma cell line, were incubated with a mouse monoclonal antibody to mature T-cells, followed by a gold-labelled antibody to mouse immunoglobulins. Multiple optical sections of each sample were collected by reflection laser scanning confocal microscopy and combined into three-dimensional renderings. A (non-confocal) transmission image was generated of each field for comparative purposes. The increasing use of reflection laser scanning confocal microscopy combined with colloidal gold conjugates as biological markers will probably be of considerable advantage in cytochemical analysis.

Antigens, CD↗

Reflectance pulse oximetry at the forehead improves by pressure on the probe.

In this study, we investigated the possibility of improving reflectance (back-scatter) pulse oximetry measurements by pressure applied to the probe. Optimal signal detection, with the probe applied to an easily accessible location, is important to prevent erroneous oxygen saturation readouts. At the foreheads of 10 healthy adult volunteers, the effects of pressure applied onto the reflectance pulse oximeter probe were studied. Distances between the LEDs (660 nm and 940 nm) and the three photodiodes in the sensor were 4 mm, 7 mm, and 10 mm. For each detector, recordings were evaluated regarding red-to-infrared (R/IR) ratios and pulse sizes in relation to the stepwise increased pressure applied to the probe. R/IR variability decreased with applied pressures between 60 and 120 mm Hg. These findings are partly attributed to a corresponding increase in red and infrared pulse sizes at the detectors, which results in an improved signal-to-noise ratio. It is thought that pressure onto the oximeter sensor forces venous blood out of the tissues underneath the sensor. Consequently, the disturbing influence of pulsating and non-pulsating venous blood is reduced. Moreover, the increased difference in vessel diameter between diastole and systole and the corresponding difference in light absorption and an increase in flow velocities, causes an increase in pulse size with increasing pressure on the probe. Pressure applied to the probe may be useful in increasing the accuracy of reflectance pulse oximetry.

Adult↗

The effect of pulsating arteries on reflectance pulse oximetry: measurements in adults and neonates.

OBJECTIVE: The objective of our study was to describe the results from human experiments during normoxia that demonstrate the effect of pulsating arteries on the measured arterial oxygen saturation (SpO2) using a reflectance pulse oximeter sensor. METHODS: In 6 healthy adults and 7 healthy neonates, a Nellcor reflection sensor (FS-10 oxisensor, Nellcor, Inc., Pleasanton, CA) was placed in three different positions: (1) on the forehead, (2) on the temporal area, with the photodiode placed over the superficial temporal artery, and (3) on the temporal area, with the light-emitting diodes (LEDs) placed over the superficial temporal artery. RESULTS: Placement of the sensor in position 2 resulted in a significantly lower SpO2 reading, compared to sensor position 1: 5.8% (p < 0.01) lower for adults and 7.5% (p < 0.01) lower for neonates. Placement of the sensor in position 3 resulted in significantly larger plethysmographic signals, compared to sensor position 1; but, the Spo2 readings were alike. CONCLUSIONS: Pulsating arteries can affect the reliability of reflection pulse oximetry. Depending on the position of the sensor, a falsely low Spo2 value can be observed.

Adult↗

Determination of reflection coefficients for various ions and neutral molecules in sarcoplasmic reticulum vesicles through osmotic volume change studied by stopped flow technique.

Osmotic volume change of sarcoplasmic reticulum vesicles was studied by following the change in light-scattering intensity using a stopped flow apparatus. From the analysis of the initial rate of scattering change, reflection coefficients for various ions and neutral molecules were determined. The following are typical results: K+, 0.72; Tris+, 0.98; choline 1; NO3-, 0.32; Cl-, 0.46; methanesulfonate, 0.62; gluconate, 0.96; glycerol, 0.86; and glucose, 1. When the K+ permeability was increased in the presence of 10(-6) g valinomycin/ml, the reflection coefficient for K+ changed from 0.72 to 0.31. It was found that there was a close relationship between the reflection coefficients and the permeabilities of the solutes. Hydraulic conductivity was also determined from the initial rate of light scattering change and was not different for the different solutes. The water permeability was estimated to be 2.1 x 10(-3) cm/sec at 23 degrees C.

Animals↗

Feasibility of non-invasive measurement of tissue pH using near-infrared reflectance spectroscopy.

OBJECTIVE: Tissue pH measurement has a number of clinical applications, including the monitoring of both muscle pH and organ pH as an indicator of compromised blood flow and anaerobic metabolism. The objective of this work was to demonstrate the feasibility of a noninvasive measurement of deep tissue pH using near-infrared reflectance spectroscopy and multivariate calibration techniques. METHODS: Six studies were done on five New Zealand white rabbits. Two pH electrodes were implanted in the teres major muscle and a vascular clamp placed across the single artery feeding the muscle. Reflected light was collected through the skin from a site between the two electrodes as the pH was lowered by closing the clamp and raised by opening the clamp. Partial least squares analysis with cross-validation techniques was used to relate pH to light absorption at 201 evenly spaced wavelengths between 700 and 1100 nm. RESULTS: On average, the tissue pH started at 7.13 +/- 0.09 and decreased to 6.74 +/- 0.09, returning to 7.13 +/- 0.09 after reperfusion. Calibration models fit for each rabbit had an average of nine factors with an R2 of 0.98 and a prediction error of 0.016 +/- 0.002 pH units. CONCLUSIONS: We believe this to be the first in vivo demonstration of a non-invasive method for measuring tissue pH in skin-covered muscle using near-infrared reflectance spectroscopy and multivariate calibration techniques.

Animals↗

Device for eliminating corneal light reflections during recording of lateral images with a slit lamp.

Corneal reflections produce defects in photographic sections of the lens taken with a slit-lamp biomicroscope for computerized densitometric analysis of cataract opacity. A simple and workable, adjustable antireflection device was built that can be easily adapted to photographic slit lamps, a common instrument in ophthalmology equipment. The slit lamp is a versatile tool for photographing structures in the anterior segment of the eye, particularly the lens. Corneal reflections are eliminated for angles between the light band and a photograph plane ranging from 20 degrees to 90 degrees, with the light band no greater than 0.5 mm and the corneal curvature about 7.6 to 7.9 mm. The device acts by blocking aberrant light rays from the light source that would otherwise be reflected by the mirrorlike surface of the cornea and enter the objective lens. Here we present a prototype designed for the Zeiss slit lamp.

Cornea↗

Assessment of cartilage thickness utilising reflectance spectroscopy.

A new principle for cartilage layer thickness assessments in joints is presented. It is based on the differences between the absorption spectra of cartilage and subchondral bone (containing blood). High-resolution ultrasound measurements of cartilage thickness were compared with reflection spectroscopy data from the same area of bovine hip joint condyles. A simple mathematical model allowed calculation of thickness and comparison with ultrasound data. The cartilage thickness was changed by being ground in short episodes. For thicker cartilage layers, a high degree of reflection in the 400-600 nm wavelength interval was seen. For thinner cartilage layers, the characteristics of the spectra of blood and bone dominated those of cartilage. The mean (+/- SD) thickness of intact cartilage was 1.21 +/- 0.30 mm (n = 30). In an exponential regression model, spectroscopic estimation of cartilage thickness showed a correlation coefficient of r = 0.69 (n = 182). For thinner cartilage layers (d < 0.5 mm), the mean model error was 0.19 +/- 0.17 mm. Results from a bi-layer Monte Carlo simulation supported the assumption of an exponential relationship between spectroscopy data and reference ultrasound data. The conclusion is that optical reflection spectroscopy can be used for cartilage layer thickness assessment.

Animals↗