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[Quantity-frequency scale (QF Scale) for adolescent problem drinking].

From 1990 to 1992, we surveyed 14,438 Japanese high school students from nine prefectures on drinking behavior. Based on this survey, we developed a new Quantity-Frequency Scale (QF Scale) for adolescent problem drinking. Each adolescent is scored from 0 to 6 by the QF Scale, which is composed of scores of drinking quantities and frequencies. Adolescents were assessed as normal adolescent, drinkers and problem drinkers by QF Scale scores. The normal adolescent (score 0) categorized by the QF Scale drinks none or little and obeys the Law Prohibiting Adolescents from Drinking. The drinker (score 1-3) categorized by the QF Scale drinks at least several times a year, but drinks little each time. The problem drinker (score 4-6) categorized by the QF Scale drinks several times a week and has at least two drink (1 drink is equivalent to a glass of beer) each time, once a week with at least 3 drinks, or once or twice a month with at least 6 drinks each time. The problem drinkers show a physical, psychological and social high risk for alcohol. The validity of the QF Scale was examined. The results showed that the problem drinkers had significantly frequent blackout, significantly frequent drinking when they had emotional conflicts or they were alone, and drank significantly more hard liquor than the drinkers and normal adolescents. Among the high school students surveyed, 39.5% were normal adolescents, 43.2% were drinkers and 17.4% were problem drinkers. In conclusion, the QF Scale is a useful instrument for assessment of adolescent problem drinking.

Adolescent↗

Antidipsogenic effect of endotoxin in the rat.

Escherichia coli LPS (640-1280 mcg/Kg, intravenously) showed a powerful, dose-dependent and long-lasting inhibitory effect on drinking behavior stimulated by 48 h water deprivation, subcutaneous injection of isoprenaline (33.3 mcg/Kg) and intracerebroventricular injection of hypertonic NaCl (2.9%) solution, carbachol (250 ng) and angiotensin II (100 ng). The antidipsogenic effect was neither a consequence of behavioral alterations, nor due to endotoxin peripheral vasodilating activity. Naloxone (60-120-240 mcg/Kg, subcutaneously) did not influence endotoxin inhibition of water intake, thus ruling out an endorphin-mediated effect. When prostaglandin synthesis was inhibited, the effect of endotoxin was reduced. Since prostaglandins do not seem to be involved in the regulation of thirst, the mechanism of the antidipsogenic effect elicited by endotoxin needs further study.

Angiotensin II↗

Altered drinking responses in dogs with chronic metabolic alkalosis.

Chronic chloride depletion alkalosis in dogs causes a lowered osmotic threshold and increased sensitivity for vasopressin (AVP) release. Since AVP release and drinking behavior normally are closely associated over a narrow range of changes in plasma osmolality (Posm), we investigated whether alkalotic dogs would also show an altered responsiveness to the dipsogenic effects of angiotensin II (ANG II) and osmotic stimuli. Dogs made chronically alkalotic by a combination of chloride-free diet and furosemide injections developed polydipsia in the absence of any increase in solute intake and in the presence of a significant reduction in Posm. The animals were chronically hypochloremic, hyponatremic and hypokalemic, and appeared to be extracellular fluid (ECF) contracted. Plasma renin activity (PRA) was 10-fold higher in alkalotic dogs than controls. When Posm was increased by a slow 2 hr infusion of hypertonic sodium sulfate, alkalotic dogs were found to have a significantly lower osmotic threshold for inducing drinking (289.8 +/- 1.1 mOsm/kg/H2O vs. 305.1 +/- 1.3 mOsm/kg/H2O in controls), but the slope or sensitivity of the water intake/Posm relationship was not significantly different. Finally, compared to normal animals, alkalotic dogs were unresponsive to the dipsogenic effects of IV ANG II. These data indicate that the central mechanisms which mediate drinking in response to cellular and extracellular thirst stimuli are altered in chronic metabolic alkalosis.

Alkalosis↗

Circadian rhythms in vitamin B12 content of the rat brain.

The whole brain content of vitamin B12 (VB12) in the rat was assayed by the chemiluminescent immunoassay at 4 h intervals in 12:12 h light-dark cycles (LD) and five different circadian times (CTs) under constant dim illumination (dim LL). In LD-entrained rats, the content of VB12 exhibited a day-night rhythm with a peak 2 h after the dark onset time and a trough 2 h before the light onset time. In freerunning rats under dim LL, the content of VB12 also exhibited a circadian variation with a peak ca. 2 h after the activity onset time (CT14) and a trough ca. 12 h after the activity onset time (CT 0). These findings clearly indicate that the brain VB12 content decreased during the active phase and increased during the resting phase regardless of the lighting schedule. On the other hand, the drinking behavior, as an index of the intake activity, was observed less frequently in the light phase of LD cycles and the resting phase of dim LL. Since most of the digested VB12 is known to be continuously stored in the liver, it is demonstrated that the rhythm of the brain content of VB12 may be caused by brain consumption of VB12 during the active phase and the transportation from the peripheral storage during the resting phase, independent of intake activities.

Animals↗

The effects of peptide and nonpeptide antagonists of angiotensin II receptors on the noradrenaline uptake of different brain structures in rats with angiotensin II-induced increase of water intake.

Angiotensin II (ANG II) significantly increased noradrenaline (NA) uptake by cortical, hypothalamic and hippocampal synaptosomes thus activating noradrenergic neurotransmission. ANG II did not affect NA uptake by striatal synaptosomes. The interaction between AT1 receptors and noradrenergic neurons and the involvement of brain noradrenergic neurotransmitter system in ANG II-induced drinking in rats is suggested by the increase of NA uptake in hypothalamus and frontal cortex which are rich in AT1 receptors and are of importance for drinking behavior. The ANG II-receptor antagonists losartan, EXP 3174, sarmesin and saralasin decreased NA uptake in all brain regions studied as compared to the uptake in the same brain regions of ANG II-injected animals thus antagonising the effect of ANG II. There is no relationship between the inhibition of ANG II-induced water intake and the changes of NA uptake under the effect of the ANG II-receptor antagonists.

Analysis of Variance↗

Centrally infused atrial natriuretic polypeptide attenuates exaggerated salt appetite in spontaneously hypertensive rats.

We have previously shown that atrial natriuretic polypeptide is present in the brain with the highest concentration in the hypothalamus and septum and that intracerebroventricular injection of atrial natriuretic polypeptide inhibits water drinking induced by centrally injected angiotensin II or 24-hour water deprivation in rats. To study further the role of brain atrial natriuretic polypeptide in the control of water and electrolyte balance, the effect of chronic intracerebroventricular infusion of atrial natriuretic polypeptide on salt appetite in spontaneously hypertensive rats and normotensive Wistar-Kyoto rats was examined with a free-choice, two-bottle preference test. The intracerebroventricular infusion of 100 ng/hour and 500 ng/hour of alpha-human atrial natriuretic polypeptide preferentially suppressed the intake of 0.30 M NaCl solution and attenuated the elevated preference for the hypertonic saline in spontaneously hypertensive rats while centrally infused alpha-human atrial natriuretic polypeptide had no significant effects on drinking behavior in Wistar-Kyoto rats. Blood pressure did not change significantly throughout the experiment in either rat strain. It is concluded that the exaggerated salt appetite in spontaneously hypertensive rats is blunted by centrally administered atrial natriuretic polypeptide. Such an effect of atrial natriuretic polypeptide along with its antidipsogenic effect suggests that brain atrial natriuretic polypeptide plays a role in water and electrolyte homeostasis and in blood pressure control.

Animals↗

Subfornical organ efferents to neural systems for control of body water.

The subfornical organ, a circumventricular structure of the central nervous system, has efferent neural projections to sites within the brain known to be involved in drinking behavior and secretion of antidiuretic hormone. By using anterograde tracing techniques, it is shown that the subfornical organ projects to the nucleus medians of the medial preoptic area, to the organum vasculosum of the lamina terminalis, and to the supraoptic nuclei bilaterally. Its efferent connectivity is confirmed by retrograde transport of horseradish peroxidase. The organum vasculosum of the lamina terminalis, another circumventricular organ and a suspected receptor site for angiotensin II, is involved in the circuitry of the subfornical organ and also has an efferent projection to the supraoptic nuclei.

Animals↗

Central dipsogenic effect of synthetic rat atrial natriuretic polypeptide in normotensive rats.

The effect of intracerebroventricular (i.c.v.) injection of synthetic rat atrial natriuretic polypeptide (alpha-rANP) on drinking behavior was studied in normotensive rats, alpha-rANP (0.2, 0.4 or 0.8 micrograms in 5 microliter) caused a dose-dependent dipsogenic effect which was abated by i.c.v. pretreatment with saralasine (9 micrograms in 5 microliter). These results suggest that alpha-rANP possesses dipsogenic effects in water repleted rats and that brain angiotensin is involved. In addition, our data indicate that, at least as far as the effect of cerebral ANP is concerned, there are some differences between alpha-rANP and human atrial natriuretic polypeptide.

Animals↗

Microinjection of sulpiride into the nucleus accumbens increases ethanol drinking in alcohol-preferring (P) rats.

The effects of dopamine antagonists microinjected into the nucleus accumbens (Acb) on alcohol-drinking behavior were studied in the selectively bred alcohol-preferring (P) line of rats. P female rats (N = 8) were given access to food and water ad lib, while availability of a 10% (v/v) ethanol solution was limited to 1 hr/day. After implantation of guide cannulas bilaterally into the Acb and recovery from surgery, the rats were microinjected with either the D1 antagonist SCH 23390 (0.1 to 2.0 micrograms/side), the D2 antagonist sulpiride (0.1 to 2.0 micrograms/side) or vehicle, and ethanol intake was monitored. The D1 antagonist SCH 23390 had little effect on alcohol intake although the 0.5 ug/side dose produced a small increase which was not statistically significant. The D2 antagonist sulpiride increased, in a dose dependent manner, the intake of ethanol to as high as 215% of control values (F(4,28) = 39.9; p < 0.001). Additional experiments indicated that the 2.0 micrograms/side dose of sulpiride did not alter the consumption of a 14% glucose nor a 0.25% saccharin solution. These data suggest that D2 receptors in the Acb are important in the regulation of alcohol drinking by the P line of rats.

Alcohol Drinking↗

The effect of the tobacco settlement and smoking bans on alcohol consumption.

In the last few years, the price of cigarettes has increased considerably in the USA. In addition, a number of states have also imposed smoking bans. These increases in the cost and barriers to smoking have created a natural experiment to study relationships between smoking and drinking behaviors. In this study, we employ data from the first six waves of the Health and Retirement Survey (HRS) to analyze the effects of smoking bans and cigarette prices on alcohol consumption. We also test if past cigarette and alcohol consumption affect current alcohol consumption as predicted by co-addiction models. We estimate dynamic panel models using GMM estimators. Our approach allows us to obtain consistent estimates irrespective of the number of time periods. The three main findings of this study are: (1) there is positive reinforcement effect of past cigarette consumption on current alcohol consumption, (2) smoking bans reduce alcohol consumption and (3) there is a positive effect of cigarette prices on alcohol consumption.

Aged↗

Food availability and daily biological rhythms.

Restricted daily feeding schedules result in the partial or complete synchronization of a wide range of rhythmic biological functions in rodents. In some cases, exemplified by drinking behavior and liver tyrosine transaminase activity, this represents primarily a direct, exogenous influence of food intake. In others, synchronization is achieved by entrainment of a circadian time-keeping mechanism distinct from that which underlies free-running rhythms in these functions. This food-entrained mechanism is responsible for the timing of anticipatory increases in locomotor and lever-pressing activity immediatley prior to food delivery, and may also underlie similar anticipatory increases in body temperature, corticosterone secretion, and in the activities of some intestinal enzymes. It is suggested that such a mechanism may enable an animal to recognize and take advantage of the periodic recurrence of significant events in its biotic environment.

Animals↗

Circadian properties of vasopressin and melatonin rhythms in cat cerebrospinal fluid.

Using a method for continuous removal of cisternal cerebrospinal fluid (CSF) from freely moving cats, we delineated the circadian nature of the daily rhythm in CSF arginine vasopressin. The daily melatonin rhythm was also monitored in CSF as another marker of circadian function. Under diurnal lighting conditions, both hormones exhibited prominent daily rhythms; the CSF vasopressin rhythm was characterized by high daytime values, whereas the CSF melatonin rhythm was characterized by high nighttime levels. In contrast, drinking behavior exhibited a 24-h component in only one of four animals studied. Daily CSF rhythms of vasopressin and melatonin persisted for over 78 days of study in constant light. The vasopressin rhythm clearly free-ran in this environment, manifesting cycle lengths of slightly greater than 24 h. The daily melatonin pattern split into several components with increasing time in constant light. An acute 8-h phase delay in the daily light-dark cycle resulted in corresponding but gradual phase shifts in both rhythms. These results indicate that both the vasopressin and melatonin rhythms in cat CSF are endogenously generated and are entrained by the daily light-dark cycle.

Animals↗

Submandibular and parotid salivary secretion after electrolytic lesioning of the brainstem nucleus parvocellularis in the rat.

The present study, in consonance with recent anatomical investigations, demonstrates that activation of the nucleus parvocellularis in the rat evokes a potent hypersecretory effect in the submandibular and sublingual (S-S) salivary glands. Furthermore, electrolytic lesioning of this region in conjunction with peripheral removal of the parotid glands is followed by an increase in the number of drinking responses in the presence of dry food. Such prandial drinking behavior is only observed after total impairment of salivation (i.e., removal of the S-S + parotid glands), thus suggesting that the parvocellularis lesion led to a marked deficit in S-S salivary secretion. On the other hand, the activation of the nucleus parvocellularis was seen to have only a slight effect on parotid salivary secretion. Electrolytic lesions to this zone, when associated with peripheral removal of the S-S glands, failed to induce prandiality, suggesting that the parvocellularis nucleus exerted a low level of control over parotid salivary secretion. These results are interpreted as functional proof of the relationship between the parvocellularis reticular formation and the superior salivatory nucleus in the secretion of S-S saliva.

Animals↗

Adipsia-polydipsia induced by simultaneous lesion of the medioventral septum and anteroventral third ventricle area in the rat.

The medioventral septal area (MVS) and also the tissue surrounding the periventricular preoptic-hypothalamic region (AV3V) of male rats, were destroyed by mean of electrolytic lesions. Before and after the lesions, daily water and food intakes, diuresis, body weight, urine osmolarity, and sodium and potassium excretion were determined. Rats with simultaneous AV3V-MVS lesions showed a biphasic pattern of drinking behavior characterized by a first period of adipsia followed by another period of polydipsia. During the first period of adipsia and except for the first two days, postlesion rats were able to reduce total urine volume but failed to produce an appropriate concentrated urine. During the polydipsia period, on the contrary, rats increased urine output and decreased urine osmolarity in a parallel fashion. Immediately after the lesion, food intake was decreased but recovered to pre-lesion levels gradually. By contrast, body weight was decreased during the entire period of the experiment. Sodium but not potassium excretion showed a significant increase from the 9th to the 20th day postlesion. The results suggest that the AV3V and MVS are part of a circuitry subserving the control of water intake and electrolyte balance.

Animals↗

Enhanced effects of central angiotensin II on cardiovascular and drinking responses in inbred polydipsic (STR/N) mice.

STR/N, an inbred strain of mice, is known to exhibit extreme polydipsia and polyuria. The objective of this study was to investigate the possible reasons for polydipsia. First, comparisons were made between STR/N mice and control mice from the Institute of Cancer Research (ICR) concerning daily drinking, urinary excretion, and basal cardiovascular function. Then, since angiotensin II (ANG II) is a potent stimulus for drinking behavior, we investigated the effects of intracerebroventricular (i.c.v.) administration of ANG II on cardiovascular and water intake responses. Daily water intake, food intake, urinary volume, and urinary electrolytes (Na and K) excretion were larger in STR/N mice than in ICR mice, and the basal blood pressure was significantly lower in STR/N mice than in ICR mice. The i.c.v. administration of ANG II (10 pmol/per mouse) resulted in increased mean arterial blood pressure (MAP) and water intake in both STR/N and ICR mice, but the changes in MAP were significantly larger in STR/N mice than in ICR mice. These results suggest that polydipsia in STR/N mice is at least partially attributable to high sensitivity of central ANG II receptors and low MAP.

Angiotensin II↗

Effects of psychoactive drugs in the Vogel conflict test in mice.

This study examined effects of various psychoactive drugs on the Vogel conflict test, where drinking behavior is punished by electric shocks, in ICR mice to clarify the pharmacological features of this method in mice. A benzodiazepine anxiolytic diazepam and a barbiturate pentobarbital produced significant anticonflict effects, which mean that these drugs increased the number of electric shocks mice received during 40-min test session. On the other hand, yohimbine (alpha2-receptor antagonist), caffeine (adenosine-receptor antagonist), scopolamine (muscarinic cholinergic antagonist), cyclazocine (sigma-receptor antagonist), cimetidine (H2-receptor antagonist), baclofen (GABA(B)-receptor agonist), MK-801 (NMDA-receptor antagonist), buspirone (5-HT1A-receptor agonist), chlorpromazine (dopamine-receptor antagonist) and haloperidol (dopamine-receptor and sigma-receptor antagonist) all did not produce anticonflict effects in this test using ICR mice. The results suggest that the Vogel conflict test is applicable to ICR mice and that this test in mice is appropriate as a screening method for drugs that have apparent anti-anxiety actions.

Animals↗

Manipulation of potential perinatal zeitgebers for the juvenile circadian temperature rhythm in rats.

We investigated the importance of pre- and postnatal maternal rhythmicity for the development and synchronization of the juvenile circadian core temperature (Tc) rhythm in rats by evaluating the Tc of artificially reared pups in six litters derived from mothers maintained in continuous bright light (LL) and impregnated after drinking behavior stopped showing circadian periodicity. Pups removed from their aperiodic mothers on postnatal day 9 and artificially reared for 3 days showed a free-running Tc rhythm whose amplitude (3.1 +/- 0.1 degrees C, n = 47) was only slightly smaller than that of control pups born to mothers maintained in a 12:12-h light-dark cycle. In four litters the acrophases were not much less synchronized than in the four control litters (mean vector lengths 0.79 +/- 0.05 vs. 0.94 +/- 0.04). In two litters synchronization among littermates was not significant. Additional experiments with cross-fostered pups showed that synchronization is not caused by the time of birth. We conclude that synchronization among littermates can develop even when maternal rhythmicity has been suppressed even before conception, possibly because the Tc decrease of several littermates happening to have similar phases at birth acts as zeitgeber.

Animals↗

Preabsorptive vs. postabsorptive control of ethanol intake in C57BL/6J and DBA/2J mice.

Experimentally naive male mice of both strains were exposed to a two-bottle choice situation (ethanol vs. water) and their drinking behavior was observed during the first hour. DBA/2J mice developed a significant avoidance of 2% or 10% ethanol during the first 10 min. At 15 and 60 min following introduction of the bottles, no DBA mouse exhibited more than a 6 mg% blood ethanol level while all of the C57BL mice exceeded this concentration. Significant postabsorptive effects in the DBA mice seem unlikely at these very low blood ethanol values. Animals of both strains were examined for their ability to form lithium-induced conditioned taste aversions to 2% ethanol or 15% sucrose solutions. DBA mice readily formed conditioned aversions to both solutions, but the C57BL strain significantly avoided only the sucrose. C57BL mice appear to have difficulty in discriminating the 2% ethanol from distilled water. The neural sensitivity to ethanol was examined in both strains using the sleep time test and the grid test. C57BL mice were significantly more sensitive than DBA mice in both tests.

Alcohol Drinking↗