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[Sedimentation properties of virion RNA of Newcastle disease virus after annealing under different conditions].

After annealing at high temperatures 50S RNA of Newcastle disease virus was partially degraded. The resulting fragments had the sedimentation coefficient of 23--32S. Solution of dimethylsulfoxide and formamide were used to decrease the temperature of annealing. However, annealing with denaturating agents caused agglomeration of 50S RNA molecules. This phenomenon is discussed.

Centrifugation, Density Gradient↗

[Immunogenic properties of 3 local lentogenic strains of the Newcastle disease virus used in the spray method of vaccination].

Three local lentogenic strains of the Newcastle disease virus, GT, D and R, were made use of to produce live liquid vaccines. These proved harmless and immunogenic when applied through the spray-method to broiler chicks, aged 5 days, at three successive revaccinations (on the 25th, 60th and 140th day of age) carried out by the same method. The strain D vaccine induced serum antihemagglutinins that had higher titers than those induced by vaccines of the other local strains and the standard strains Hitchner-B1 and La Sota.

Animals↗

Radioiodination of the envelope proteins of Newcastle disease virus.

Lactoperoxidase-catalyzed iodination selectively labels the two glycoproteins (VP1 and VP2) of Newcastle disease virus. The low-molecular-weight, nonglycosylated major viral protein, VP6, was not iodinated in the intact virus but was iodinated in disrupted virions, suggesting a localization on the inner, rather than the outer, envelope surface. Studies on the distribution of virion proteins labeled with 125-I and 3-H-isoleucine between detergent-soluble and detergent-insoluble fractions show that the virion proteins VP4, VP5, and VP6 are solubilized to a much lesser extent than are VP1 and VP2.

Animals↗