Newcastle disease virus-neutralizing indexes of normal chicken serums.
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The migration of peritoneal exudate cells collected from chickens immunized against Newcastle disease virus was inhibited by the inactivated virus antigen. The agarose droplet method for macrophage migration-inhibition test in chickens is described.
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After annealing at high temperatures 50S RNA of Newcastle disease virus was partially degraded. The resulting fragments had the sedimentation coefficient of 23--32S. Solution of dimethylsulfoxide and formamide were used to decrease the temperature of annealing. However, annealing with denaturating agents caused agglomeration of 50S RNA molecules. This phenomenon is discussed.
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Three local lentogenic strains of the Newcastle disease virus, GT, D and R, were made use of to produce live liquid vaccines. These proved harmless and immunogenic when applied through the spray-method to broiler chicks, aged 5 days, at three successive revaccinations (on the 25th, 60th and 140th day of age) carried out by the same method. The strain D vaccine induced serum antihemagglutinins that had higher titers than those induced by vaccines of the other local strains and the standard strains Hitchner-B1 and La Sota.
Lactoperoxidase-catalyzed iodination selectively labels the two glycoproteins (VP1 and VP2) of Newcastle disease virus. The low-molecular-weight, nonglycosylated major viral protein, VP6, was not iodinated in the intact virus but was iodinated in disrupted virions, suggesting a localization on the inner, rather than the outer, envelope surface. Studies on the distribution of virion proteins labeled with 125-I and 3-H-isoleucine between detergent-soluble and detergent-insoluble fractions show that the virion proteins VP4, VP5, and VP6 are solubilized to a much lesser extent than are VP1 and VP2.