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PubMed1984

Dihomogammalinolenic acid, but not eicosapentaenoic acid, activates washed human platelets.

Dihomogammalinolenic acid (2.5-20 microM) added to suspensions of washed human platelets induces platelet shape change and the formation of 1,2-diacylglycerol and phosphatidic acid, indicating the activation of phospholipase C. It also stimulates the phosphorylation of a 40 kDa protein, indicating the activation of protein kinase C. Dihomogammalinolenic acid is converted mainly to 12-hydroxyheptadecadienoic acid and to a smaller extent to prostaglandin E1 and thromboxane B1. Small quantities of the lipoxygenase product 12-hydroxyeicosatrienoic acid are also observed. Indomethacin, by blocking platelet cyclooxygenase, prevents the activation of phospholipase C, protein kinase C, and platelet shape change induced by dihomogammalinolenic acid. Compound UK 38485, a specific thromboxane synthetase inhibitor, does not block platelet activation induced by dihomogammalinolenic acid. The results indicate that endoperoxides derived from dihomogammalinolenic acid, such as prostaglandin G1 or prostaglandin H1, may be responsible for the stimulation of phospholipase C and protein kinase C, and for the induction of platelet shape change. Eicosapentaenoic acid does not activate platelets and is poorly metabolized by platelet cyclooxygenase and lipoxygenase. Eicosapentaenoic acid is a better inhibitor of platelet activation induced by various agonists in washed platelets than dihomogammalinolenic acid. Eicosapentaenoic acid and dihomogammalinolenic acid are, however, equally effective in inhibiting aggregation induced by collagen in platelet-rich plasma. We suggest that eicosapentaenoic acid might be a better antithrombotic agent than dihomogammalinolenic acid.

8,11,14-Eicosatrienoic Acid↗

PubMed1985

Chemo-attractant for the garter snake: characterization of vomeronasally-mediated response-eliciting components of earthworm wash--III.

Heating a 0.1 N HCl solution of lyophilized garter snake, Thamnophis sirtalis, chemoattractant obtained from earthworm, Lumbricus terrestris, surface washings, in a boiling water bath, and separation by gel chromatography, gave two fractions, F2, of high molecular weight and containing all the chemoattractant activity, and F4, of low molecular weight. The activity is lost between 15 and 30 min of heating. A similarly treated 0.1 N NaOH solution of lyophilized washings gave no F2 fraction and an F4 fraction in increasing amount. All the chemoattractant activity is lost between 0 and 15 min. Separations were monitored by assays for protein and carbohydrate.

Animals↗

PubMed1995

Detection of Pseudomonas aeruginosa from ovine fleece washings by PCR amplification of 16S ribosomal RNA.

Two oligonucleotides were selected from the variable regions of the 16S rRNA gene of P. aeruginosa and used as PCR primers for the detection of P. aeruginosa. The specificity of the primers was tested against the following bacterial species; Pseudomonas putida, Pseudomonas cepacia, Xanthamonas maltophilia, Pseudomonas mendocina, Pseudomonas stutzeri, Pseudomonas fluorescens, Pseudomonas alcaligenes and Pseudomonas diminuta. These primers had a sensitivity of detection of 1 pg of chromosomal DNA or 1 x 10(5) cfu/microliters and were species-specific. The sensitivity of detection was increased to 1 fg or less than 10 cfu/microliters using a non-radioactively labelled probe. Using these PCR primers it was possible to detect the presence of P. aeruginosa from fleece washings collected from a flock of 100 sheep. Correlation between single PCR and bacteriological isolation showed agreement in 89% of fleece samples tested, 2% of the samples contained organic PCR inhibitors in the fleece washings, 3% were below the level of sensitivity of the test and the remaining 6% were culture negative for P. aeruginosa but PCR positive. Use of nested PCR did not increase the sensitivity of detection over single round PCR combined with the use of non-radioactively labelled probe.

Animals↗

PubMed1990

Effect of thromboxane A2 synthetase inhibition, singly and combined with thromboxane A2/prostaglandin endoperoxide receptor antagonism, on inositol phospholipid turnover and on 5-HT release by washed human platelets.

Differential effects on human platelet function of thromboxane A2 (TXA2) synthetase inhibition singly and of TXA2 synthetase inhibition combined with TXA2/prostaglandin endoperoxide receptor antagonism were revealed, using ridogrel as a probe. Ridogrel combines selective TXA2 synthetase inhibition with TXA2/prostaglandin receptor antagonism in one molecule: in washed human platelets, the compound reduces the production of TXB2 (IC50 = 1.3 X 10(-8) M) and increases that of PGF2 alpha, PGE2, PGD2 from [14C]arachidonic acid. Additionally, at higher concentrations (Ki = 0.52 X 10(-6) M), it selectively antagonizes the breakdown of inositol phospholipids, subsequent to stimulation of TXA2/prostaglandin endoperoxide receptors with U 46619. The latter happens in a competitive way with fast receptor association-dissociation characteristics. At low concentrations (1 X 10(-9)-1 X 10(-7) M) producing single TXA2 synthetase inhibition, ridogrel reduces the collagen-induced formation of TXB2 by washed platelets, but enhances [32P]phosphatidic acid (PA) accumulation and [3H]5-hydroxytryptamine (5-HT) release. At higher concentrations (1 X 10(-6)-1 X 10(-5) M) which additionally block U 46619-induced [32P]PA accumulation, ridogrel inhibits the [32P]PA accumulation and release of [3H]5-HT by human platelets stimulated with collagen. These observations, corroborated by results obtained with OKY 1581, sulotroban, indomethacin and human serum albumin, suggest a causal role for prostaglandin endoperoxides in the stimulation by TXA2 synthetase inhibition of platelet reactions to collagen. They reinforce the concept that TXA2 synthetase inhibition-induced reorientation of cyclic endoperoxide metabolism, away from TXA2 into inhibitory prostanoids, requires additional TXA2/prostaglandin endoperoxide receptor antagonism to achieve optimal anti-platelet effects.

15-Hydroxy-11 alpha,9 alpha-(epoxymethano)prosta-5↗

PubMed2005

The structure of disgust: domain specificity in relation to contamination ideation and excessive washing.

The primary goal of the present study was to employ confirmatory factor analysis to compare two theoretical models regarding the factor structure of disgust (N=307). The two-factor model of Animal Reminder and Core disgust proposed by Rozin et al. (2000) demonstrated superior model fit over a one-factor model that has been implicated in prior research. However, contrary to theoretical predictions, categorizing Hygiene disgust as Core disgust rather than Animal Reminder disgust provided an overall better fit for the two-factor model. The second aim of the study was to investigate the relationship between the empirically derived two-factor model and contamination ideation and excessive washing using structural equation modeling. Findings indicated that the Contamination Ideation and Excessive Washing latent factor was specifically related to Core disgust (domain specific) and negatively related to Animal Reminder disgust. The relevance of these findings in the context of future research investigating the role of disgust in specific anxiety disorders is discussed.

Anxiety↗

PubMed2004

Clinical outcome and atherothrombogenic risk profile after prolonged wash-out following long-term treatment with high doses of n-3 PUFAs in patients with an acute myocardial infarction.

BACKGROUND: Sustained effects following withdrawal of n-3 PUFAs are unknown. METHODS: Clinical outcome [cardiac death, resuscitation, recurrent myocardial infarction (MI) or unstable angina pectoris] was assessed after prolonged wash-out following randomised treatment with high-dosed n-3 PUFAs or corn oil for 12-24 months in 300 acute MI patients. Atherothrombogenic risk markers, serum glucose and markers of lipid peroxidation and inflammation were evaluated in 89 out of the 100 last included patients. RESULTS: After a total median observation period of 45 (range 0-53) months no intergroup difference in prognosis was observed for any of the cardiac events. Favourable effects on serum triglycerides and HDL-cholesterol by n-3 PUFAs were lost after washout, but triglycerides decreased in the corn oil as compared to the n-3 group, P < 0.001. The decline in total cholesterol after withdrawal was similar in both groups. No intergroup difference in the change in thiobarbituric acid-malondialdehyde, a marker of lipid peroxidation, ultrasensitive C-reactive protein, homocysteine, glucose or blood platelets was noted at sustained follow-up. CONCLUSION: Clinical outcome was similar in both patient groups, and the atherothrombogenic risk improvement by n-3 PUFAs was lost after prolonged wash-out. Withdrawal did not affect homocysteine, glucose or markers of lipid peroxidation or inflammation.

Acute Disease↗

PubMed2006

Effects of organic and inorganic additives on flotation recovery of washed cells of Saccharomyces cerevisiae resuspended in water.

Separation of microbial cells by flotation recovery is usually carried out in industrial reactors or wastewater treatment systems, which contain a complex mixture of microbial nutrients and excretion products. In the present study, the separation of yeast cells by flotation recovery was carried out using a simple flotation recovery systems containing washed yeast cells resuspended in water in order to elucidate the effects of additives (defined amounts of organic and inorganic acids, ethanol, surfactants and sodium chloride) on the cellular interactions at interfaces (cell/aqueous phase and cell/air bubble). When sodium chloride, organic acids (notably propionic, succinic and acetic acids) and organic surfactants (sodium dodecyl sulphate (SDS), cetyltrimethylammonium bromide (CTAB) and Nonidet P40) were added to the flotation recovery system, significant increases in the cell recovery of yeast hydrophobic cells (Saccharomyces cerevisiae, strain FLT-01) were observed. The association of ethanol to acetic acid solution (a minor by-product of alcoholic fermentation) in the flotation recovery system, containing washed cells of strain FLT-01 resuspended in water, leading to an increased flotation recovery at pH 5.5. Thus, the association among products of the cellular metabolism (e.g., ethanol and acetic acid) can improve yeast cell recovery by flotation recovery.

Acetic Acid↗

PubMed2004

Chelator induced phytoextraction and in situ soil washing of Cu.

In a soil column experiment, we investigated the effect of 5 mmol kg(-1) soil addition of citric acid, ethylenediamine tetraacetate (EDTA), diethylenetriamine-pentaacetate (DTPA) and [S,S]-stereoisomer of ethylenediamine-disuccinate (EDDS) on phytoextraction of Cu from a vineyard soil with 162.6 mg kg(-1) Cu, into the test plant Brassica rapa var. pekinensis. We also examined the use of a horizontal permeable barrier, composed of layers of nutrient enriched sawdust and apatite, for reduction of chelator induced Cu leaching. The addition of all chelators, except citric acid, enhanced Cu mobility and caused leaching of 19.5-23% of initial total Cu from the soil column. However, Cu plant uptake did not increase accordingly; the most effective was the EDDS treatment, in which plant Cu concentration reached 37.8 +/-1.3 mg kg(-1) Cu and increased by 3.3-times over the control treatment. The addition of none of the chelators in the concentration range from 5 to 15 mmol kg(-1) exerted any toxic effect on respiratory soil microorganisms. When EDDS was applied into the columns with horizontal permeable barriers, only 0.53 +/- 0.32% of the initial total Cu was leached. Cu (36.7%) was washed from the 18 cm soil layer above the barrier and accumulated in the barrier. Our results indicate that rather than for a reduction of Cu leaching during rather ineffective chelate induced Cu phytoextraction, horizontal permeable barriers could be more effective in a new remediation technique of controlled in situ soil washing of Cu with biodegradable chelates.

Biodegradation, Environmental↗

PubMed2005

Biodegradation and speciation of residual SS-ethylenediaminedisuccinic acid (EDDS) in soil solution left after soil washing.

This paper aims to investigate the degradation and speciation of EDDS-complexes (SS-ethylenediaminedisuccinic acid) in soil following soil washing. The changes in soil solution metal and EDDS concentrations were investigated for three polluted soils. EDDS was degraded after a lag phase of 7-11 days with a half-life of 4.18-5.60 days. No influence of EDDS-speciation on the reaction was observed. The decrease in EDDS resulted in a corresponding decrease in solubilized metals. Changes in EDDS speciation can be related to (1) initial composition of the soil, (2) temporarily anoxic conditions in the soil slurry after soil washing, (3) exchange of EDDS complexes with Cu even in soils without elevated Cu and (4) formation of NiEDDS. Dissolved organic matter is important for metal speciation at low EDDS concentrations. Our results show that even in polluted soils EDDS is degraded from a level of several hundred micromoles to below 1 microM within 50 days.

Chelating Agents↗

PubMed2005

Telomere length on bladder washing samples from patients with bladder cancer correlates with tumor characteristics flow cytometry method for quantitative fluorescence in situ hybridization (flow-FISH technique).

OBJECTIVE: The purpose of the present study was to evaluate the length of telomeres in patients with bladder cancer using a quantitative flow cytometry (flow-FISH) technique. METHODS: Bladder washing samples from 51 patients with bladder cancer were obtained immediately before transurethral resection. The average length of telomere repeats was measured by flow-FISH, as previously reported. Results were expressed in molecular equivalents of soluble fluorochrome (MESF) units. RESULTS: Bladder washing specimens provided adequate cell numbers for flow-FISH in 49 cases. The TEL means were 1014.71, 2343.36, 5567 and 18267.57 for Ta, T1, T2 and T3/4 tumors, respectively. Regarding grade it was obtained a mean MESF value of 1379.46, 3391.29 and 15925.11 for G1, G2 and G3, respectively. ANOVA demonstrated statistically significant differences in stage (p: 0.014) and tumor grades (p: 0.012). In relation to ploidy, we found a mean MESF value of 2701.37 and 16085.44 MESF units for diploid and aneuploid cells, respectively. Significant difference (p: 0.003) was observed between both groups. CONCLUSION: To date, this is the first report wherein telomere length was measured using flow-FISH method in exfoliated cells in urine from patients with bladder cancer. Further investigations are required to demonstrate whether flow-FISH technique might be considered as a tumor marker of bladder cancer.

Analysis of Variance↗

PubMed2006

Skin decontamination: Importance of the wash-in effect.

That skin washing/decontamination may increase percutaneous absorption is commonly referred to as the 'wash-in' (W-I) effect. This article traces the development of the W-I effect both in vivo and more recently in vitro. The mechanism(s) responsible for this effect are examined particularly in relation to the laboratory method used in vitro. The importance of the W-I effect is presented and it is recommended that caution be practiced when skin is washed as the W-I effect may increase both local cutaneous and general systemic toxicity. Experimental data on a wide variety of chemicals are urgently needed.

Animals↗

PubMed2006

Comparing the Multi-ZSC one-step standardized swim-up method to the double-wash swim-up method with regard to the effects of sperm separation on morphology, head morphometry, and acrosome reaction inducibility.

The objective of this study was to test the efficacy of the new Multi-ZSC system on human sperm separation compared with the commonly used double-wash swim-up method. It was found that the double-wash swim-up method selects sperm with better head morphometry and acrosome reaction inducibility than the upper fractions of the Multi-ZSC.

Cell Culture Techniques↗

PubMed2026

Chemical and sensory profiling of fermented, washed, and artificially flavored coffee beans: Insights into flavour quality, authenticity, and food safety implications.

This study establishes an integrated framework combining chemical profiling, sensory analysis, and molecular mechanism evaluation to compare flavour quality and authenticity among fermented, washed, and artificially flavored coffees. GC × GC-TOF-MS and UHPLC-HRMS showed that fermented samples had markedly higher ester and aromatic alcohol levels (total esters 74.5 ± 7.8 mg kg-1; phenylethanol 27.5 ± 3.2 mg kg-1, p < 0.01), enhancing fruity-floral notes. Washed coffees contained the highest organic acid concentrations (45.2 ± 3.8 mg kg-1, p < 0.01), supporting brightness and umami. Artificially flavored coffees exhibited elevated exogenous aromatics (vanillin 21.5 ± 3.1 mg kg-1) but significantly fewer Maillard products (p < 0.05) and reduced flavour retention (55% after 14 days). Molecular docking revealed higher theoretical binding affinities for naturally generated compounds, suggesting a potential molecular basis for their greater sensory persistence. The framework supports constructing coffee quality fingerprints and verifying flavour authenticity.

Flavoring Agents↗

PubMed2004

Amphetamines in washed hair of demonstrated users and workplace subjects.

In a study of volunteer subjects from drug rehabilitation programs, methamphetamine and amphetamine levels were determined in the hair of 40 subjects who had produced MS-confirmed methamphetamine-positive urine results. The samples were tested by radioimmunoassay and analyzed by LC/MS/MS after being washed with the 3.75-h wash procedure developed by this laboratory. In addition, results of non-user and workplace samples are presented. In workplace samples, levels of methamphetamine, amphetamine, methylenedioxy-methamphetamine (MDMA), and methylenedioxyamphetamine (MDA), are reported. The range of methamphetamine levels in the clinical samples (170-34,400 pg/mg hair) was not different from the workplace population (from less than the cutoff of 500 pg/mg to >20,000 pg/mg hair), but the workplace population had a lower percentage of high levels of drug. Amphetamine levels were found to vary widely in both populations, at all levels of methamphetamine. In the clinical population, no samples were positive for MDMA; in MDMA-positive workplace samples, the levels ranged from below the cutoff of 500 to >20,000 pg/mg, with MDA levels varying widely, similar to amphetamine levels in methamphetamine-positive samples.

Adult↗

PubMed2005

Reducing Salmonella on cantaloupes and honeydew melons using wash practices applicable to postharvest handling, foodservice, and consumer preparation.

Washing conditions that included a soak or brush scrub were evaluated for removal of Salmonella from the surface of smooth (honeydew) or complex (cantaloupe) melon rinds. Melon rinds were spot-inoculated onto a 2.5 cm2 area of rind (squares) with approximately 6.0 log(10) CFU/square of an avirulent nalidixic acid-resistant strain of Salmonella typhimurium. Melons were washed by immersion in 1500 ml of water or 200 ppm total chlorine and allowed to soak or were scrubbed over the entire melon surface with a sterile vegetable brush for 60 s. Inoculated sites, uninoculated sites ("next to" sites) that were adjacent to inoculated sites, and sites on the side of the melon opposite (remote sites) the inoculated site were excised and pummeled in a stomacher for 2 min prior to plating onto tryptic soy or bismuth sulfite agar supplemented with 50 microg/ml nalidixic acid. S. typhimurium was reduced on the rind of cantaloupe by 1.8 log CFU/melon after soaking for 60 s in 200 ppm total chlorine, which was significantly better than the 0.7 log CFU/melon achieved with soaking in water. For both water and 200 ppm total chlorine, scrubbing with a vegetable brush was shown to be significantly (0.9 log CFU/cantaloupe) more effective than soaking alone. When honeydew melons were soaked or scrubbed in water, reductions of 2.8 log CFU/melon or >4.6 log CFU/melon (four of five samples), respectively, were observed. However, when water treatments were used, the presence of Salmonella-positive "next to" and remote sites indicated that bacteria were spread from inoculated site on the rind to uninoculated sites either through the rinse water (40-70 CFU/ml of Salmonella) or scrub brush (400-500 CFU/brush). Transfer to other sites occurred more often with cantaloupe than honeydew melons. This transfer was eliminated when 200 ppm total chlorine was used. When 200 ppm total chlorine was used, Salmonella could not be detected in the water or on the scrub brush. For optimal microbial removal in food service and home settings, melons should be scrubbed with a clean brush under running water. However, to ensure the benefits of brushing, instructions for cleaning and sanitizing brushes must also be emphasized. For food service settings where concentration and pH can be adequately measured, the use of chlorinated water may provide additional benefit.

Chlorine↗

PubMed2006

A soft-solution process for recovering rare metals from metal/alloy-wastes by grinding and washing with water.

We have developed a novel process for recovering metals from alloy-wastes by using a mechanochemical (MC) reaction. The process consists of co-grinding both alloy and polyvinyl chloride (PVC) samples, followed by washing with water and filtration. The co-grinding of the wastes causes a solid-state MC reaction to form metal chlorides and hydrocarbon in the product. The former products are soluble in water, so they can be recovered from the wastes by washing with water, followed by filtration. The PVC waste plays a significant role as a chlorine source in the MC reaction. After filtration, the solid residue can be used as a fuel, due to the absence of chlorine in the product, and the filtrate is subjected to hydrometallurgical process to extract metals from the solution.

Alloys↗

PubMed2004

Efficacy of alcohol-based gels compared with simple hand wash and hygienic hand disinfection.

A recent research letter on the limited efficacy of alcohol-based hand gels has alerted the global infection control community and raised the question of the true significance of data obtained according to EN 1500. It has been described that a 1 min simple hand wash reduces artificial contamination of hands by a log(10) reduction factor of 2.8 and a 1 min reference hand disinfection with 2-propanol (60%, v/v) by a factor of 4.6 steps. The EN 1500 gel data show that the 30 s efficacy of most gels is closer to a simple hand wash than to the reference hand disinfection. The 30 s efficacy of most alcohol-based liquid products and one gel, however, is almost identical to the reference hand disinfection. In many European countries alcohol-based liquid products have been established as a standard practice in hygienic hand disinfection for decades. Replacement of these products with most available gels would be a step backward in terms of efficacy and has still to be seen critically from the efficacy point of view.

Anti-Infective Agents, Local↗

PubMed2006

Reduction of carry-over in column-switching HPLC/MS system with automated system washing procedure for highly sensitive direct analysis of donepezil in dog plasma.

To solve the problem of carry-over--a persistent chromatographic challenge for bioanalytical assays with highly sensitive detector such as a mass spectrometry (MS)--a new on-line sample pretreatment HPLC/MS system using a column-switching technique was established. This system was designed to reduce carry-over based on a hydrophobic interaction mechanism using a washing function (multi-mobile phase flow system), as well as to remove impurities on-line in a mobile phase for the pretreatment. As a result, a washing function in this system was enabled to reduce carry-over and to remove impurities in a mobile phase by automatic operation. Therefore, concentration levels of donepezil (DH) in dog plasma as low as 10 pg/mL could be determined using this on-line sample pretreatment HPLC/MS system. In addition, method validation results of specificity, linearity, accuracy, precision, lower limit of quantitation (LLOQ), and carry-over demonstrated that this on-line sample pretreatment HPLC/MS system was robust and valid as a practical assay of drugs and metabolites in the biological samples.

Animals↗