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Summary of the 1992 EPA/A&WMA International Symposium: measurement of toxic and related air pollutants.

A joint conference cosponsored for the seventh year by the Atmospheric Research and Exposure Assessment Laboratory (AREAL) of the U.S. Environmental Protection Agency (EPA) and the Air & Waste Management Association (A&WMA) was held in Durham, North Carolina, May 4-8, 1992. The technical program consisted of 200 presentations held in 23 technical sessions and covered recent advances in ambient and source measurement techniques for air pollutants. The symposium was attended by almost a thousand professionals from the United States and other countries, covered a wide range of measurement topics, and was supported by 90 exhibitors of instrumentation and consulting services. This overview highlights the technical presentations of the symposium.

Air Pollutants↗

Preparation of cross-linked cellulases and their application for the enzymatic production of glucose from municipal paper wastes.

Hydrolysis of cellulosic wastes has been applied for environmental purposes and glucose production. An enzymatic process is proposed for such treatment of municipal cellulosic wastes, and the optimum conditions are described. It was found that different conditions should be applied for the treatment of soft or hard paper wastes, the most characteristic being pretreatment of wastes and temperature of the treatment process. Optimization of enzyme characteristics was also examined after stabilization of the enzymes by cross-linking. Endocellulase was better stabilized after cross-linking with EDAC whereas, exocellulase was better with glutaraldehyde. The application of cross-linked enzyme in the waste paper treatment process resulted in about a 25% increase of glucose liberation.

Buffers↗

Results of a survey to assess curriculum needs for academic training programs in hazardous substances.

With the advent of new industrial hygiene academic training programs in hazardous substances, located at nine United States universities, a curriculum needs assessment survey was developed at the University of Minnesota and completed by members of the joint American Industrial Hygiene Association/American Conference of Governmental Industrial Hygienists Hazardous Waste Committee and members of the Health and Safety Subcommittee of the Hazardous Waste Action Coalition. The survey asked respondents to indicate whether 27 course subjects (1) should be required, (2) may be required or elective, (3) should be elective, or (4) should not be offered. Respondents were also asked to recommend additional course subjects, research needs in the hazardous materials and waste field, and certifications to be pursued. Results from 50 completed surveys (50% response rate) showed that about half of the respondents work for consulting companies and have more than 15 years of experience in the hazardous material or waste field. The courses receiving the most "required" responses included (1) the relevant regulations, (2) worker training in health and safety, (3) waste management technology, (4) pollution prevention, and (5) treatment technology. Of elective subjects, respondents recommended education in communications, public affairs, negotiations and conflict resolution, property inspection, government relations, and air pollution modeling. Respondents indicated the strongest research needs were in the areas of direct reading instruments (improved versions and their use for personal exposure monitoring) and heat stress management of personnel wearing protective clothing. Respondents felt that the best qualifications to pursue after graduation were the Certified Industrial Hygienist designation alone, or in combination with the Certified Safety Professional designation.

Curriculum↗

Tigecycline-resistant Staphylococcus in waiting pens of a pig slaughterhouse: genomic insights into a food safety alert.

BACKGROUND: The waiting pens of slaughterhouses represent a critical control point in the 'farm-to-fork' continuum, yet their role in the emergence and dissemination of antimicrobial resistance remains understudied. This study investigated tigecycline-resistant Staphylococcus (TRS) in these high-risk zones to assess their prevalence, resistance mechanisms, and transmission dynamics. METHODS: 400 samples were collected from the waiting pens of a pig slaughterhouse in Guangzhou, China. Antimicrobial susceptibility testing, whole-genome sequencing, phylogenetic analysis, and molecular cloning were employed to characterize resistance mechanisms and transmission patterns. RESULTS: 78 TRS strains were isolated and classified into three species, including S. borealis, S. ureilyticus, and S. pasteuri. These isolates exhibited multidrug-resistant phenotypes and carried new mutations in rpsJ and tet(M), which were functionally confirmed to reduce tigecycline susceptibility. Phylogenetic evidence demonstrated clonal transmission between pig farms and the slaughterhouse. The tet(M) gene was located within Staphylococcal cassette chromosome mec elements mediated by IS257, while tet(L) was carried by plasmids formed through IS256/IS257-mediated recombination. CONCLUSIONS: Waiting pens serve as crucial reservoirs for the amplification and dissemination of antimicrobial resistance. Our findings underscore the urgent need for enhanced biosecurity measures, improved waste management, and routine molecular surveillance in these high-risk zones to mitigate the spread of resistance along the food production chain.

Animals↗

Developmental sites and relative abundance of immature stages of the stable fly (Diptera: Muscidae) in beef cattle feedlot pens in eastern Nebraska.

A 3-yr study was done to determine where and at what relative frequency stable fly, Stomoxys calcitrans (L.), immatures develop in beef cattle feedlot pens and the relationship of stable fly immature versus adult sample densities. Pens within feedlots were divided into five areas (the feed apron, back fence, side fences, mound, and the general lot); from each area, three core samples were randomly taken weekly. In 1986 and 1987, the feed apron yielded the most immature stable flies (62.5%). The mound and side fences yielded a significant percentage of flies (24.6 and 8.4%, respectively). There was a strong correlation (overall r = 0.86) between numbers of immatures and numbers of adults 2 wk later. In 1988, a drought year, low numbers of immatures were collected, and only one correlation between numbers of immatures and adults was significant. In all 3 yr, sample densities of stable fly immatures peaked and began to decline by midseason. Waste management along the feed apron and mound areas could significantly reduce stable fly populations. Sampling immatures from these areas could permit prediction of adult numbers.

Animals↗

'Cold SSCP': a simple, rapid and non-radioactive method for optimized single-strand conformation polymorphism analyses.

A rapid (< 2.5 hrs) method for single-strand conformation polymorphism (SSCP) analysis of PCR products that allows the use of ethidium bromide staining is described. PCR products ranging in size from 117 to 256 bp were evaluated for point mutations and polymorphisms by 'cold SSCP' in commercially available pre-cast polyacrylamide mini-gels. Several electrophoretic parameters (running temperature, buffers, denaturants, DNA concentration, and gel polyacrylamide concentration) were found to influence the degree of strand separation and appeared to be PCR fragment specific. Use of the 'cold' SSCP technique and the mini-gel format allowed us to readily optimize the electrophoretic conditions for each PCR fragment. This greatly increased our ability to detect polymorphisms compared to conventional, radioisotope-labeled 'hot' SSCP, typically run under two standard temperature conditions. Excellent results have been obtained in resolving mutant PCR fragments from human p53 exons 5 through 8, human HLA-DQA, human K-ras exons 1 and 2, and rat K-ras exon 3. Polymorphisms could be detected when mutant DNA comprised as little as 3% of the total gene copies in a PCR mixture. Compared to standard 'hot' SSCP, this novel non-isotopic method has additional advantages of dramatically increased speed, precise temperature control, reproducibility, and easily and inexpensively obtainable reagents and equipment. This new method also lacks the safety and hazardous waste management concerns associated with radioactive methods.

Animals↗

Assessment of occupational doses from internal contamination with 241Am.

A group of workers with occupational intakes of 241Am, which occurred a long time ago, has been followed for some time. Results of in vivo measurement and bioassay of excreta are compared with the values predicted by the ICRP Publication 78 model. The observed skeletal content is, as a rule, higher than the predicted one. The ratio of excreted activity in urine to that in faeces is in very good agreement with the model prediction. Another group of workers from a waste management department, who were internally contaminated in July 2001, has also been followed. In some cases, there is quite a large difference in calculated intake between excretion by urine and that by faeces. The contaminant was presumably the same as that in the group of workers with old intakes, but its physical and chemical form could be influenced by a fixating lacquer used to prevent the spread of contamination.

Aged↗

Why should we do environmental dose reconstructions?

Environmental dose reconstructions are being conducted at many nuclear weapons production and testing sites in the United States. These projects involve reconstructing potential radiation exposures and doses from past releases of radionuclides to people who lived near nuclear facilities where these releases occurred. The results of dose reconstructions can be used as the basis for deciding if epidemiologic studies or other public health activities should be undertaken. In addition, the results of dose reconstruction can be used in other risk assessment activities, such as those associated with environmental restoration and radioactive waste management. For example, the historical inventory of hazardous materials used and potentially released at the site can be used to begin to assess the risk for exposures from current and future activities. The site-specific environmental dosimetry methods developed for dose-reconstruction purposes are applicable to other environmental risk assessments performed for that site. These and other benefits of environmental dose reconstructions are discussed.

Environmental Exposure↗

Review of historical monitoring data on Techa River contamination.

The Mayak Production Association was the first Russian site for the production and separation of plutonium. The extensive increase in plutonium production during 1948-1955, as well as the absence of reliable waste-management technology, resulted in significant releases of liquid radioactive effluent into the rather small Techa River. This resulted in chronic external and internal exposure of about 30,000 residents of riverside communities; these residents form the cohort of an epidemiologic investigation. Analysis of the available historical monitoring data indicates that the following reliable data sets can be used for reconstruction of doses received during the early periods of operation of the Mayak Production Association: Temporal pattern of specific beta activity of river water for several sites in the upper Techa region since July 1951; average annual values of specific beta activity of river water and bottom sediments as a function of downstream distance for the whole river since 1951; external gamma-exposure rates near the shoreline as a function of downstream distance for the whole Techa River since 1952; and external gamma-exposure rate as a function of distance from the shoreline for several sites in the upper and middle Techa since 1951.

Environmental Monitoring↗

Radiation safety standards and their application: international policies and current issues.

This paper briefly describes the current policies of the United Nations Scientific Committee on the Effects of Atomic Radiation and the International Commission on Radiological Protection and how these policies are converted into international radiation safety standards by the International Atomic Energy Agency, which is the only global organization-within the United Nations family of international agencies-with a statutory mandate not only to establish such standards but also to provide for their application. It also summarizes the current status of the established corpus of such international standards, and of it foreseeable evolution, as well as of legally binding undertakings by countries around the world that are linked to these standards. Moreover, this paper also reviews some major current global issues related to the application of international standards, including the following: strengthening of national infrastructures for radiation safety, including technical cooperation programs for assisting developing countries; occupational radiation safety challenges, including the protection of pregnant workers and their unborn children, dealing with working environments with high natural radiation levels, and occupational attributability of health effects (probability of occupational causation); restricting discharges of radioactive substances into the environment: reviewing current international policies vis-a-vis the growing concern on the radiation protection of the "environment;" radiological protection of patients undergoing radiodiagnostic and radiotherapeutic procedures: the current International Action Plan; safety and security of radiation sources: post-11 September developments; preparedness and response to radiation emergencies: enhancing the international network; safe transport of radioactive materials: new apprehensions; safety of radioactive waste management: concerns and connections with radiation protection; and radioactive residues remaining after the termination of activities: radiation protection response to the forthcoming wave of decommissioning of installations with radioactive materials. The ultimate aim of this paper is to encourage information exchange, cooperation, and collaboration within the radiation protection professional community. In particular, the paper tries to facilitate consolidation of the growing international regime on radiation safety, including the expansion of legally binding undertakings by countries, the strengthening of the current corpus of international radiation safety standards, and the development of international provisions for ensuring the proper worldwide application of these standards, such as a system of international appraisals by peer review.

Government Agencies↗

Lead exposure in radiator repair workers: a survey of Washington State radiator repair shops and review of occupational lead exposure registry data.

Radiator repair workers in Washington State have the greatest number of very elevated (> or =60 microg/dL) blood lead levels of any other worker population. The goals of this study were to determine the number of radiator repair workers potentially exposed to lead; estimate the extent of blood lead data underreporting to the Occupational Lead Exposure Registry; describe current safety and health practices in radiator repair shops; and determine appropriate intervention strategies to reduce exposure and increase employer and worker awareness. Lead exposure in Washington State's radiator repair workers was assessed by reviewing Registry data and conducting a statewide survey of radiator repair businesses. This study revealed that a total of 226 workers in Washington State (including owner-operators and all employees) conduct repair activities that could potentially result in excessive exposures to lead. Approximately 26% of radiator repair workers with elevated blood lead levels (> or =25 microg/dL) were determined to report to Washington State's Registry. This study also revealed a lack of awareness of lead's health effects, appropriate industrial hygiene controls, and the requirements of the Lead Standard. Survey respondents requested information on a variety of workplace health and safety issues and waste management; 80% requested a confidential, free-of-charge consultation. Combining data derived from an occupational health surveillance system and a statewide mail survey proved effective at characterizing lead exposures and directing public health intervention in Washington State.

Data Collection↗

Wastewater minimization in indirect electrochemical synthesis of phenylacetaldehyde.

Wastewater minimization in phenylacetaldehyde production by using indirect electrochemical oxidation of phenylethane instead of the seriously polluting traditional chemical process is described in this paper. Results show that high current efficiency of Mn(III) and high yield of phenylacetaldehyde can be obtained at the same sulfuric acid concentration (60%). The electrolytic mediator can be recycled and there will be no waste discharged.

Acetaldehyde↗

Survival of E. coli O157:H7 in organic wastes destined for land application.

AIM: To determine the persistence of Escherichia coli O157 in contrasting organic wastes spread to land and to assess the potential environmental risk associated with the disposal of these wastes to land. METHODS AND RESULTS: Twenty-seven organic wastes originating from slaughterhouses, wastewater treatment plants (raw and treated sewage), creameries and farms (bovine slurry), were inoculated with E. coli O157:H7 and incubated at 10 degrees C. Although pathogen numbers gradually declined in all the wastes, albeit at different rates even in the same waste type, E. coli O157:H7 was still viable in 77% of organic wastes tested after 2 months. CONCLUSIONS: Long-term storage of organic wastes led to a significant and gradual decline in E. coli O157:H7 numbers. Consequently, storage may be a useful means of reducing the pathogen load of wastes destined for land application. However, in most cases, long-term storage cannot be expected to completely eliminate E. coli O157:H7 from waste. SIGNIFICANCE AND IMPACT OF THE STUDY: Our results indicate that current legislation may be insufficient to protect the environment from E. coli O157:H7 contamination from untreated wastes spread to land.

Abattoirs↗

Airborne fungi and their secondary metabolites in working places in a compost facility.

The present study investigates in detail the exposure to airborne fungal spores in working places in compost facilities. The fungal species composition in the air is compared with the spectrum of microbial metabolites with regard to the physiological properties of different fungal species. The results indicate that the spectrum of microbial volatile organic compounds (MVOCs) and the production of mycotoxins can be specific for certain species. In addition to the pathogenic and allergological relevance, the fungi may have different toxicological health impacts.

Air Microbiology↗

Perception and acceptance of technological and environmental risks: why are poor countries less concerned?

The research has been aimed at answering two questions: (1) What factors impact perception and acceptance of technological and environmental hazards? (2) Why are rich societies involved more in protecting their environment and health than poor societies? Data has been collected from representative samples of two countries--Poland and Sweden. The results indicate that (1) contrary to earlier findings, the inverse relations between perceived benefits and dangers of hazards has not been observed, (2) acceptance of a risk has been mostly influenced by perceived benefits, (3) rejection of a risk has been mostly influenced by its perceived harmful consequences. Concerning the second question, it has been found that: (1) perceived hazard's danger and benefit is not the only factor that impacts its acceptance, and (2) a broader economic context can impact acceptance (tolerance) of hazards. It has been found that being aware of high dangers and not very high benefits of hazardous activities, Poles still have accepted them. Thus, Poles seem to follow an old proverb: "When one does not have what one likes, one has to like what one has."

Adult↗

Occupational safety and health standards.

If we are to approach developing a safe and healthful workplace in a more timely fashion, a more generic approach must be considered and applied instead of developing recommendations and standards simply on a substance-by-substance basis, an approach that has been the most prominent. Some examples in which developing generic standards may be appropriate are: cholinesterase-inhibiting substances, neurotoxic agents, reproductive hazards, cold environments, and vibration syndrome, to name but a few. It is important to recognize that developing standards based on individual substances often does not allow for the role of synergism, a reaction that has had little study, but it is important in controlling occupational disease and injury. These concerns can be addressed in several ways. One is to look at processes or conditions found in the workplace; for example, coke oven emissions that OSHA has promulgated into a standard and, as NIOSH has done in their recommendations to OSHA for foundries, coal tar products, the manufacture of paint and allied coatings, field sanitation, hazardous waste management, hot environments, and confined spaces. Another is to address groups of similar substances such as NIOSH has done with alkanes, benzidine-based dyes, diisocyanates, dinitrotoluenes, and glycol ethers. A third comprehensive approach is to look at general categories of hazards, such as the generic carcinogen policy, and the hazard communication rule. Finally, risk must be considered in the development of any standard. Nelson Rockefeller once said in relation to an incidence involving a radiation hazard that, "you can't have a riskless society." I would amend this to say that you cannot have a reckless society either. Safety and health regulations are essential and must be designed, promulgated, and then enforced so that a reckless society is avoided or controlled, with a riskless society being the ultimate aim.

Humans↗

Analysis of Escherichia coli O157:H7 survival in ovine or bovine manure and manure slurry.

Farm animal manure or manure slurry may disseminate, transmit, or propagate Escherichia coli O157:H7. In this study, the survival and growth of E. coli O157:H7 in ovine or bovine feces under various experimental and environmental conditions were determined. A manure pile collected from experimentally inoculated sheep was incubated outside under fluctuating environmental conditions. E. coli O157:H7 survived in the manure for 21 months, and the concentrations of bacteria recovered ranged from <10(2) to 10(6) CFU/g at different times over the course of the experiment. The DNA fingerprints of E. coli O157:H7 isolated at month 1 and month 12 were identical or very similar. A second E. coli O157:H7-positive ovine manure pile, which was periodically aerated by mixing, remained culture positive for 4 months. An E. coli O157:H7-positive bovine manure pile was culture positive for 47 days. In the laboratory, E. coli O157:H7 was inoculated into feces, untreated slurry, or treated slurry and incubated at -20, 4, 23, 37, 45, and 70 degreesC. E. coli O157:H7 survived best in manure incubated without aeration at temperatures below 23 degreesC, but it usually survived for shorter periods of time than it survived in manure held in the environment. The bacterium survived at least 100 days in bovine manure frozen at -20 degreesC or in ovine manure incubated at 4 or 10 degreesC for 100 days, but under all other conditions the length of time that it survived ranged from 24 h to 40 days. In addition, we found that the Shiga toxin type 1 and 2 genes in E. coli O157:H7 had little or no influence on bacterial survival in manure or manure slurry. The long-term survival of E. coli O157:H7 in manure emphasizes the need for appropriate farm waste management to curtail environmental spread of this bacterium. This study also highlights the difficulties in extrapolating laboratory data to on-farm conditions.

Aerobiosis↗

Influence of seasonal environmental variables on the distribution of presumptive fecal Coliforms around an Antarctic research station.

Factors affecting fecal microorganism survival and distribution in the Antarctic marine environment include solar radiation, water salinity, temperature, sea ice conditions, and fecal input by humans and local wildlife populations. This study assessed the influence of these factors on the distribution of presumptive fecal coliforms around Rothera Point, Adelaide Island, Antarctic Peninsula during the austral summer and winter of February 1999 to September 1999. Each factor had a different degree of influence depending on the time of year. In summer (February), although the station population was high, presumptive fecal coliform concentrations were low, probably due to the biologically damaging effects of solar radiation. However, summer algal blooms reduced penetration of solar radiation into the water column. By early winter (April), fecal coliform concentrations were high, due to increased fecal input by migrant wildlife, while solar radiation doses were low. By late winter (September), fecal coliform concentrations were high near the station sewage outfall, as sea ice formation limited solar radiation penetration into the sea and prevented wind-driven water circulation near the outfall. During this study, environmental factors masked the effect of station population numbers on sewage plume size. If sewage production increases throughout the Antarctic, environmental factors may become less significant and effective sewage waste management will become increasingly important. These findings highlight the need for year-round monitoring of fecal coliform distribution in Antarctic waters near research stations to produce realistic evaluations of sewage pollution persistence and dispersal.

Animals↗