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Carcase and meat quality in broilers either killed with a gas mixture or stunned with an electric current under commercial processing conditions.

1. Carcase and meat quality were evaluated under commercial conditions in 400 broilers either killed with a mixture of 30% carbon dioxide and 60% argon in air or stunned with a 50 Hz AC with clipped sine wave. 2. Compared with electrical stunning, killing broilers with the gas mixture eliminated or substantially reduced the prevalence of carcase and meat quality defects. 3. The results also showed that killing broilers with a mixture of 30% carbon dioxide and 60% argon would enable filleting (deboning) to be performed at 4 h post mortem without adversely affecting the cook loss or texture of breast meat.

Abattoirs↗

Influence of activity and dietary energy on broiler performance, carcase yield and sensory quality.

1. A total of 2560 male and female Ross broilers were raised to 42 days of age in a 2 X 2 treatment factorial arrangement experiment to investigate the influence of different degrees of physical activity and dietary energy on broiler performance, abdominal fat content, carcase yield and sensory quality. 2. Vertical fans were used to force the treatment birds to walk 3 to 4 times as far as the normal activity birds: birds were fed a normal and a high energy diet (12.55 compared with 13.81 MJ ME/kg) with the same energy/protein, energy/lysine and energy/methionine + cystine ratios. 3. High activity birds had greater body weight (+4.1%), food intake (+5.1%) and ME intake (+5.1%) than normal activity birds. Birds receiving high energy diet had a lower food conversion and food intake than birds receiving normal energy diet. There were no significant differences in body weight or ME intake between birds with different diets. 4. Slaughter yields, both absolute and relative to live body weight, were affected by activity or dietary energy to varying degrees. Breast meat was increased with more activity. The absolute weight of abdominal fat was independent of activity and in males the relative weight of abdominal fat was decreased in high activity birds. 5. Different degrees of activity and dietary energy had only minor influences on broilers sensory quality.

Animals↗

Comparative studies on the histochemical properties of M. iliotibialis lateralis from Kumamoto Cochin crossbred roaster and broiler chickens.

1. Histochemical properties of M. iliotibialis lateralis were compared between Kumamoto Cochin (a Japanese native breed) crossbred roasters (KC roasters, 112 d of age) and normal broilers (56 d of age). 2. Myofibres were divided into Types II-R, II-I and II-W showing high, moderate and low reduced nicotinamide adenine dinucleotide dehydrogenase (NADH-DH) activities, respectively. 3. Subsarcolemmal formazan granules indicating very strong NADH-DH activity in Type II-R fibres were observed only in KC roasters. In both sexes the percentage of Type II-R fibres present was greater in KC roasters than in broilers. 4. These results indicated marked differences in histochemical properties and meat quality between KC roasters and broilers.

Animals↗

Shackling of broilers: effects on stress responses and breast meat quality.

1. Experiments were conducted to study the welfare and meat quality effects of shackling. In experiment 1, broilers with or without leg problems were shackled (S) for 4 min on a moving line and blood sampled; or handled (H), returned to the crate and sampled after 4 min; or sampled immediately after removal from the crate (control, C). 2. Plasma corticosterone (CORT) concentrations, as measured by radioimmunoassay, were highest in S and lowest in C, while the H group was intermediate. Leg problems had no effect on CORT. 3. In experiment 2, tonic immobility (TI) was induced in broilers after 2 min inverted handling to determine fear responses. One week later, the birds were fasted, transported and then shackled on a moving shackle line for 0, 1, 3 or 4 min, then unshackled and blood sampled. Wing flapping during shackling was also quantified. 4. Shackling time did not influence CORT concentrations. There was a negative correlation (r = -0.714) between CORT and wing flapping duration in the 1 min shackling treatment. There was no relationship between TI and wing flapping or CORT. 5. In experiment 3, broilers were exposed to two food withdrawal (FW) times (food withdrawn overnight or during crating only) and held for 4 h prior to processing, shackled (0, 2 or 4 min shackling time, ST), and then killed by exsanguination. Blood samples were collected during the neck-cut. Pectoralis superficialis and Supracoracoideus samples were either collected after 15 min and individually quick frozen (IQF) in liquid nitrogen or collected at 4 h post mortem from carcases chilled on slush ice (COI). 6. CORT increased significantly with increased ST. There was a FW x ST interaction effect on the initial pH of fillets. ST influenced the b*, chroma and Hue values of the COI fillets. FW influenced the L* and Hue values of both IQF and COI fillets as well as the a* value of the COI fillets. 7. In summary, CORT increased with shackling time when birds were held after transport. FW and ST also influenced the colour of fillets, although it is not clear whether these changes are perceptible to the consumer. The duration of wing flapping during shackling did not appear to be related to fearfulness, although it was influenced by properties of the shackle line. We suggest that there be a maximum time lapse between shackling and stunning or killing of 2 min to minimise stress and meat quality changes.

Abattoirs↗

Effect of clenbuterol on growth, carcase and skeletal muscle characteristics in broiler chickens.

1. Male and female broiler chickens (144 in total) were given diets supplemented with clenbuterol (CB) at 0 (control) and at 1 mg/kg between 28 and 49 d of age to study the effect of CB on growth, carcase and skeletal muscle. 2. CB improved growth in males by increasing daily weight gain and final live weight and by lowering food conversion ratio. In females it changed the carcase composition by reducing abdominal fat pad and by increasing the proportion of protein. Consequently, carcase protein gain was increased in both sexes (11% and 16%, respectively). 3. Skeletal muscle weights were enhanced by between 6% and 22%. Muscle fibre diameters were increased in extensor hallucis longus (EHL) but not in gastrocnemius (GAS) muscle. This increase was more pronounced in females. EHL total muscle fibre number remained unchanged. The proportion of fast-twitch glycolytic fibres was increased at the expense of fast-twitch oxidative fibres in males only. Nuclear/cytoplasm and DNA/protein ratios tended to be decreased by CB. 4. From the elevated EHL muscle RNA/DNA, unchanged protein/RNA and translation activity it is suggested that CB stimulated protein synthesis at the pretranslational level. Reduced protein degradation is deduced from decreased neutral calcium-dependent proteolytic activity. 5. It is concluded that broiler chickens respond to long-term CB treatment as has been shown in various mammals. However, the sex-specific response in growth, carcase composition and skeletal muscle cellularity is more clearly apparent in broiler chickens.

Adrenergic beta-Agonists↗

Effects of dietary sorghum of different tannin concentrations and tallow supplementation on the performance of broiler chicks.

1. The chemical compositions and tannin contents of 17 varieties of grain sorghum were determined. 2. Commercial broiler chicks were fed from 7 to 49 d of age on 15 experimental diets which varied in their concentrations of tannins and animal fat (tallow), by the inclusion of different amounts of high and a low protein varieties of sorghum. 3. Substitution of a low tannin sorghum completely for maize did not have any significantly adverse effect on performance. Broiler chicks could tolerate up to 2.6 g tannin/kg above which their performance was reduced. Addition of tallow did not significantly affect broiler performance except at the highest tannin content and added fat intake, where weight gain and efficiency appeared to improve significantly.

Adipose Tissue↗

Effects of parent Shamo cocks on the histochemical properties of M. iliotibialis lateralis and M. supracoracoideus on their crossbred broilers.

1. Four Shamo (a Japanese game bird) cocks showing different characteristics in the histochemical properties of M. iliotibialis lateralis (ITL) were crossed with White Rock hens to produce male and female crossbred broilers of the 4 lines (90 d of age). Normal broilers (56 d) were used, for comparison. 2. Histochemical properties of ITL and M. supracoracoideus (SC) were compared among the crossbred lines and normal broilers. Myofibres were divided into Types II R, II I and II W showing high, moderate and low reduced nicotinamide adenine dinucleotide dehydrogenase (NADH-DH) activities, respectively. 3. In the ITL of the crossbred cockerels, the percentage of Type II R and II I fibres decreased and conversely Type II W increased in comparison to those in the Shamo. 4. Sex differences of the histochemical properties were recognised only in the ITL of the crossbred, in which the percentage of Type II R fibres was greater in the male. 5. The different characteristics of the parent Shamo cocks were reproduced only in the different fibre type composition of the ITL muscle in the crossbred cockerels. 6. The histochemical features of fibre type seemed to develop with bird age, particularly subsarcolemmal accumulation of formazan granules (indicating high NADH-DH activity) in Type IIR fibres. 7. Breed, line, sex and age differences in the histochemical properties were demonstrated clearly in ITL but not in SC.

Age Factors↗

Genetic parameters of body weight of broiler chickens measured at 22 degrees C or 32 degrees C.

1. Male broilers (n = 1521) from 247 families were reared from 4 to 6 weeks of age at 22 degrees or 32 degrees C. 2. Genetic correlations between measurements recorded at 22 degrees C and 32 degrees C were 0.73 +/- 0.12 for weight gain between 4 and 6 weeks and 0.74 +/- 0.08 for food conversion ratio (FCR). Genes controlling weight gain at both temperatures differed to some extent. 3. Heritability of weight gain from 4 to 6 weeks was much lower at 32 degrees C than at 22 degrees C (0.13 +/- 0.03 vs 0.24 +/- 0.04): selection for increased body weight will thus be much less efficient at 32 degrees C than at 22 degrees C. 4. Conversely, heritabilities of the FCR were very similar at the 2 temperatures (0.28 +/- 0.04 at 22 degrees C and 0.27 +/- 0.04 at 32 degrees C). Selecting for FCR would thus be efficient at 32 degrees C too. 5. These results justify, at least under our experimental conditions, selecting broiler lines for improved growth performance at 22 degrees C. However, it could be more efficient if broilers are to be reared in hot climates to select for improved FCR rather than for increased body weight.

Animal Nutritional Physiological Phenomena↗

Prevalence and control of foot-pad dermatitis in broilers in Sweden.

1. A cross-sectional observational study was carried out to investigate spatial, temporal and management aspects of foot-pad dermatitis in Swedish broilers. The efficacy of a control programme was evaluated. 2. Flock information on producer, breed, foodstuff manufacturer, region, abattoir, date of slaughter, age at slaughter, planned and actual stocking density was recorded. A total of 6988 flocks, representing approximately 110 million broilers was examined. A total of 175 broiler producers from 15 geographical regions is represented. 3. The total foot-pad score per flock ranged from 0 to 200, with a mean of 34.7 and a standard deviation of 40.9. 4. Simple linear regression analysis showed that the mean total score on a weekly basis decreased (P < 0.001) over time, mainly because of a decrease in the prevalence of severe foot-pad lesions. 5. There was an association (P < 0.05) between slaughterhouse, foodstuff supplier and geographic region respectively and the mean total foot pad score of the flocks slaughtered.

Abattoirs↗

Effectiveness of two fluoroquinolones for the treatment of chronic respiratory disease outbreak in broilers.

1. Chronic respiratory disease (CRD) caused by Mycoplasma synoviae in association with Escherichia coli is the disease most frequently encountered in poultry in Mexico. 2. Differences of antimycoplasmal activity, pharmacokinetics and cost among fluoroquinolones were the impetus for this clinical evaluation of efficacy of enrofloxacin and norfloxacin-nicotinate in broilers with CRD. 3. A total of 99,600 broilers, naturally infected with M. synoviae and E. coli, were treated with enrofloxacin (10 mg/kg/d for 3 d) (n = 49,800) or norfloxacin-nicotinate (20 mg/kg/d for 3 d) (n = 49,800). 4. Based on survivor analysis, there were no significant differences of efficacy of the 2 drugs. Survivor probabilities were above 0.99. 5. These results indicate that enrofloxacin and norfloxacin-nicotinate are efficacious for treatment of CRD. Questions remaining to be answered by other studies include: public health concern about the use of fluoroquinolones for the treatment of CRD; clinical superiority of one of these drugs on the basis of an attribute other than antimicrobial activity, such as cost:benefit ratio or ability to prevent bacterial resistance. 6. Clinical efficacy is not a relevant variable in assessing whether norfloxacin-nicotinate or enrofloxacin should be used for the treatment of CRD associated with E. coli in broilers.

Animals↗

Progressive bradycardia, a possible factor in the pathogenesis of ascites in fast growing broiler chickens raised at low altitude.

1. The first objective was to evaluate baseline heart rate (HR) responses in fast growing broilers fed ad libitum from 1 to 45d or subjected to a food restriction (85%, 70% and 55% of ad libitum from 7 to 21d) and a refeeding (22 to 45d) regimen in a normal thermal environment. The second was to evaluate HR and haematocrit responses in fast growing broilers reared at a low environmental temperature and fed ad libitum (A-L) from 1 to 42d, subjected to food restriction (70% ad libitum) between 7 to 21d and refed thereafter (R-R), or subjected to food restriction between 7 to 42d (R), and to follow the changes in normal birds and those prone to ascites. 2. The baseline HR in the normal thermal environment at 21 and 45d in birds fed ad libitum was lower (P < 0.001) than at 7d. The food-restricted groups had higher HRs at 21d than at 7d, with the differences significant (P < 0.01) for the 70% and 55% treatments. At 21d the HRs in restricted groups were significantly higher (P < 0.01) than in ad libitum-fed birds. After returning to ad libitum feeding the HRs in these birds tended to decline, with the differences significant (P < 0.05) for the 70% and 55% treatments. 3. In the birds exposed to cold, each feeding regimen produced distinct patterns of change in HR and haematocrit. Both feeding regimen and age had significant (P < 0.001) effects on the changes of both variables. Relative to 7d, on 14d and 21d the HRs decreased and haematocrits increased in all groups, but the rates of change were highest in the A-L and R-R birds. The first fulminant cases of ascites were observed during the third week in the A-L group and during the fourth week in the R-R group. None of the chickens from the R group developed ascites. Altogether, the incidence of ascites was 48% in the A-L group and 28% in the R-R group. 4. Among the birds exposed to cold, the ascitic birds had significantly (P < 0.001) lower HRs and higher haematocrits than normal birds from the same feeding regimen group. The time trends in the rates of change in HR and haematocrit were distinctly different (P < 0.001) for ascitic and normal birds. The change in feeding regimen from restricted to ad libitum in the R-R group resulted in a significant (P < 0.001) decrease in HR and increase in haematocrit. 5. In view of the evidence presented, it appears that hypoxaemia and tissue hypoxia in broiler chickens may be a result of circulatory insufficiency associated with progressive bradycardia rather than, as commonly believed, pulmonary insufficiency. In this situation the pulmonary hypertension may be a secondary symptom.

Analysis of Variance↗

Stunting syndrome in broilers: effect of electrolytes in drinking water on performance and intestinal glucose transport.

Posthatched naive or inoculated male broiler chicks were kept in separate rooms. An inoculum was prepared from intestines of stunting-syndrome affected broiler chicks. Tap water was supplied from 2 L cups, 1 cup per pen. In the Ist experiment, the naive chicks were provided with tap water only and the inoculated ones had free access to tap water or to an electrolyte solution. In the 2nd experiment, the naive and inoculated birds had free access to water in addition to an electrolyte solution. Supplementation was provided up to 3 weeks of age; thereafter all chicks had access to tap water only. Water or electrolyte consumption and body weight (BW) were determined. Total water intake of inoculated chicks was higher than that of naive counterparts (P<0.001). Electrolyte supplementation increased drinking (P<0.001) in inoculated birds more than in naive ones. At 1 week old the weight of the inoculated birds was about 64% of the weight of naive ones; at the age of 4 and 6 weeks it was about 74% and 86% respectively. Compensatory growth was most apparent in the inoculated chicks provided with electrolyte solution. At the age of 6 weeks, the latter exceeded the BW of the exclusively water supplied counterparts by 327 g. Electrolyte supplementation up to the age of 3 weeks had no effect on the naive counterparts. Osmolality was reduced slightly, but very significantly by inoculation; electrolyte supply had no effect on this variable. Sodium concentration in the plasma was higher in the inoculated birds. Plasma albumin was markedly reduced by inoculation on weeks 1 and 2. Whereas the inoculated chicks supplied with electrolytes resumed the level plasma albumin level of the naive chicks on week 3, an over-compensation occurred in the inoculated-water-supplied (IW) group, and they surpassed the naive chicks significantly. Blood hematocrit increased significantly with age; inoculation, age and/or electrolyte supplementation had no effect on this variable. Sodium-dependent glucose transport rates were enhanced in vesicles obtained from inoculated chicks as compared to naive ones. While electrolyte supplementation had no effect on glucose active transport in naive chicks, electrolyte supplementation decreased rates of glucose active transport in inoculated ones. These data demonstrate that electrolyte supplementation during the early age may be used to enhance the tolerance of broiler chicks to stunting-syndrome by improving food and water consumption, and subsequently growth rate during and after cessation of electrolyte supply.

Animals↗

Effect of dietary flax oil and hypobaric hypoxia on pulmonary hypertension and haematological variables in broiler chickens.

1. Three experiments were conducted with broiler chickens using hypobaric chambers and control pens, feeding diets containing 25 or 50 g flax oil/kg food or control diets with equivalent amounts of animal/vegetable (A/V) blend oil for 4 weeks. The effect of these diets on haematological variables and the extent of right ventricular hypertrophy (RVH) leading to ascites was determined. 2. Overall growth rate was not consistently affected by dietary treatment, although feeding the 25 g flax oil/kg diet reduced weight gain in week 4 of one experiment. Feeding the 50 g flax oil/kg diet but not the 25 g flax oil/kg diet reduced RVH in birds exposed to hypobaric conditions compared to feeding control diets. 3. Feeding the 50 g flax oil/kg diet under hypobaric conditions reduced the haematocrit and haemoglobin content, increased the erythrocyte deformability and the proportion of unsaturated fatty acids in the erythrocyte membranes, and reduced the whole blood viscosity compared to feeding control diets. These effects were not seen when the 25 g flax oil/kg diet was fed. The ratio of n-3 to n-6 fatty acids in erythrocyte membranes was increased in the 50 g flax oil/kg treatment group compared to controls. 4. Including 50 g flax oil/kg broiler diet reduces RVH in broiler chickens. This may be attributable in part to an increase in erythrocyte deformability from an increased proportion of unsaturated fatty acids in the erythrocyte membranes.

Animal Nutritional Physiological Phenomena↗

Sodium bicarbonate and potassium bicarbonate supplements for broilers can cause poor performance at high temperatures.

1. Supplements of between 2 and 8 g of sodium bicarbonate (NaHCO3)/l in drinking water provided between 21 and 49 d of age to broilers at 31 degrees C increased growth after 35 d of age and food intake after 42 d of age compared to unsupplemented controls. The most consistent responses were observed with 2 g NaHCO3/l. 2. Differences in weekly water intakes with different concentrations of NaHCO3 in the drinking water did not equate with variations in weekly food intake and growth. 3. Unexpected high mortality from dehydration and nephrosis was observed with a 10 g NaHCO3/l supplement. This mortality was associated with major changes in blood acid-base balance and plasma electrolyte concentrations. 4. Dietary supplements of NaHCO3 gave significantly better food conversion and numerically better growth in broilers at 31 degrees C than supplements of potassium bicarbonate (KHCO3) providing similar dietary concentrations of bicarbonate. 5. Supplements of NaHCO3 were more toxic to broiler chickens when supplied in the drinking water than in the diet. This appeared to be related to the intake of sodium and alterations to cellular membrane ionic potential.

Animal Feed↗

Performance and gastro-intestinal response of broiler chickens fed on cereal grain-based foods soaked in water.

1. Two experiments were carried out to investigate the addition of 1 3 kg water per kg air-dry mash diets containing high proportions (600 to 700 g/kg) of ground cereal grains (wheat, barley or oats) on broiler performance and the structure and function of the gastro-intestinal tract. 2. Chicks at the age of 7 d were fed on the wheat-, barley- or oats-based diets in the dry or wet forms for 35 d. Food and water intakes were recorded daily while body weight was measured weekly. Two birds from each treatment were killed each week to measure gut size and the viscosity of gut contents. Tissue samples from various digestive segments were histo-morphologically examined to determine the thickness of tissue layers, size of tissue glands, villa heights, crypt depths and thickness of tunica muscularis. Crypt cell proliferation rate (CCPR) for each segment was also determined using a metaphase arrest technique. 3. The results from both experiments showed that wetting food significantly (P<0.05) increased food intake, total water intake and body weight gain of broiler chickens. The body weight gains of birds were proportional to their food intakes so that the efficiency of food utilisation was similar for all treatments. Dry matter retention of food tended to increase in birds given wet food from 7 to 21 d but not thereafter, compared to the dry-fed birds. Although water intake from the water bottle was significantly (P<0.05) reduced in birds given wet food, total water intakes from the water bottle plus that from food were significantly (P<0.05) higher in the wet-fed birds than in the dry-fed birds. The ratio of total water to dry food intake was, however, similar in both feeding regimens. 4. The fresh empty weight of the gut was increased by wet-feeding while its relative weight to body weight and the length of gut was not affected by dietary treatments. Significantly greater development of the tissue glands in the proventriculus and gizzard was observed in the birds given wet food; this was associated with the reduced thickness of the muscular layer of these segments. An increase in villus height was also observed in duodenum, small intestine, caeca and colon of birds given wet food, compared to those given dry food. CCPR was significantly (P<0.05) reduced by wet-feeding throughout the digestive tract. This was associated with a significant decrease in the mean viscosity of the gut contents and the concentration of volatile fatty acids (VFAs) in the caeca. 5. Wetting diets based on cereal grains caused a significant improvement in the performance of broiler chickens. The mechanism of the beneficial effects of wet feeding could be attributed to the decreased viscosity of gut contents; the greater development of the layer of villi in the digestive segments and the reduced CCPR in the crypts of the epithelium.

Aging↗

Plasma lipoprotein concentration as an indicator of fatness in broilers: development and use of a simple assay for plasma very low density lipoproteins.

1. A turbidimetric assay for plasma very low density lipoproteins (VLDL) was developed, based on their selective precipitation with heparin and Mg2+. 2. Measurements of plasma VLDL concentration using the turbidimetric method showed a correlation of 0.98 with plasma VLDL plus low density lipoprotein triglyceride concentration estimated chemically. 3. Correlations between plasma VLDL concentration measured turbidimetrically and body fat content were similar to or higher than those obtained previously using a chemical assay. Correlations for broiler grandparent stock fed on a low-fat diet were 0.70 and 0.65 for males and females respectively. 4. Turbidimetric measurement of plasma VLDL concentration provides a simple, rapid and effective method of estimating fatness in live broilers that could be applied to commercial broiler breeding programmes.

Adipose Tissue↗

Influence of stocking density on the performance, carcass characteristics and selected welfare indicators of broiler chickens.

AIM: To compare the effects of four stocking densities (5, 10, 15 or 20 birds per m2) on the performance, carcass characteristics and selected welfare indicators of broiler chickens. METHODS: A total of 756 day-old male broiler chicks were assigned as six replicates of 13, 25, 38 and 50 birds per pen, to stocking densities of 2,000, 1,000, 667 and 500 cm2 floor space per bird, respectively. The birds were housed in floor pens in an environmentally controlled room. Normal commercial diets were fed ad libitum and fresh water was freely available throughout the 5-week study period. Liveweights of birds and feed intake were recorded at weekly intervals. Data on carcass parameters and welfare indicators (moisture, gait scores, feather scores, breast and hip lesions, and foot pad and hock burns) were obtained on Day 35. Adrenal glands were removed at slaughter, weighed and examined histologically to determine the total proportion of cortical and medullary tissues. RESULTS: During the study, the birds at the lowest density grew faster (p<0.001-0.01) and consumed more (p<0.001- 0.01) feed than those at the other three stocking densities. The liveweight gains and feed intake of birds reared at densities of 10, 15 and 20 birds per m2 were similar (p=0.11-0.83 and p=0.17-0.57, respectively). Stocking density had no influence on the feed/weight gain, mortality or carcass characteristics. No breast or hip lesions were observed in carcasses from any of the groups. The weight and structure of the adrenal glands were similar across all groups although the staining of medullary tissue of birds was most intense in the highest stocking density group. As stocking density increased, so did moisture and gait score, hock and foot pad burn scores, and the feather score was adversely affected. CONCLUSIONS: The data showed that the relationship between stocking density and productivity traits was not linear in nature, in accordance with other studies. Under the conditions of the current study, the performance, mortality and carcass characteristics of broilers grown at densities of 10, 15 and 20 birds per m2 were similar. There were some welfare implications at high population densities as indicated by adverse effects on litter quality, gait scores and the incidence of hock and foot pad burns.

Journal Article↗

Oral treatment with bacteriophages reduces the concentration of Salmonella Enteritidis PT4 in caecal contents of broilers.

Bacteriophages isolated from free-range chickens were tested as a therapeutic agent for reducing the concentration of Salmonella enterica serovar Enteritidis phage type 4 (S. Enteritidis PT4) in caeca of broilers. One-day-old broilers infected with S. Enteritidis PT4 by a seeder bird method were orally treated on the seventh day of age with a mixture of 10(11) plaque-forming units of each of three bacteriophages. Five days after treatment the bacteriophage-treated group showed a reduction of 3.5 orders of magnitude on colony-forming units of S. Enteritidis PT4 per gram of caecal content. Samples collected at 10, 15, 20 and 25 days after treatment revealed that treated birds still had lower colony-forming units of S. Enteritidis PT4 per gram of caecal content. These data gave us compelling evidence that a mixture of bacteriophages may be efficacious in reducing S. Enteritidis PT4 concentration in broilers' caeca and therefore reducing contamination of poultry products by this food-borne pathogen.

Animals↗