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Rethinking mental retardation: education and eugenics in Connecticut, 1818-1917.

This case study of mental retardation in Connecticut during 1818-1917 questions the existing model of interpretation. The discovery of mental retardation in Connecticut did not emanate from social fear over those who were different, difficult, or dangerous. Nor did state government initiate the institutionalization of the feeble-minded. Instead, Dr. Henry M. Knight, who founded the private Connecticut School for Imbeciles in 1858, was motivated by antebellum religious benevolence. His altruism was additionally motivated by cultural concerns to shape behavior according to middle-class, Protestant norms. By the end of the century, his son and successor Dr. George H. Knight departed from his father's emphasis on education and assimilation to embrace eugenics and segregation of the mentally retarded. Connecticut's pioneering marital ban (1895) and sterilization law (1909) were, however, virtually ineffective. Instead, the state sponsored in 1917 a large-scale custodial facility that sought to isolate the feeble-minded, whom reformers now portrayed as a menace to society. In sum, the Knights show a clear departure in policy between the first and second generation of administrators.

Attitude to Health↗

A physiological basis for biosphere-atmosphere interactions in the boreal forest: an overview.

Interdisciplinary field experiments for global change research are large, intensive efforts that study the controls on fluxes of carbon, water, trace gases, and energy between terrestrial ecosystems and the atmosphere at a range of spatial scales. Forest ecophysiology can make significant contributions to such efforts by measuring, interpreting, and modeling these fluxes for the individual components of forest ecosystems and then integrating the results into holistic ecosystem process models. The Boreal Ecosystem-Atmosphere Study (BOREAS) was undertaken because of the importance of the boreal forest biome to various global change issues. The study was conducted from 1993 to 1996 at sites in Saskatchewan and Manitoba, Canada. Results have shown that physiological processes of plants in the boreal forest can have large-scale consequences. For example, the composition of tree species strongly influences flux rates, with deciduous species having much higher carbon and water fluxes than coniferous species. Additionally, physiological limitations to transpiration in boreal conifers, even when soil water is abundant, reduces latent heat flux and increases sensible heat flux over large regions. This physiological control of transpiration can increase the depth of the atmospheric boundary layer on warm spring days to a level similar to that found in desert biomes. This special issue features 10 articles that address various aspects of the physiological basis of biosphere-atmosphere interactions in the boreal forest. The articles emphasize the environmental controls on water flux, carbon flux, and ecosystem productivity.

Journal Article↗

Biologic effects of cobalt chrome in cell and animal models.

The literature on animal and cellular models used to study the response to cobalt chrome alloy implants and wear and corrosion products is reviewed. Animal studies show that in solid form cobalt chrome alloy is relatively well tolerated. Injections of large numbers of particles in a single bolus lead to acute inflammation and necrosis, followed by a chronic inflammatory response. Macrophages are the predominant cell type and may persist in the tissues for years. Long term studies have failed to confirm the induction of tumors. In vitro studies confirm the toxic effects of cobalt chrome alloy corrosion products and wear particles, especially cobalt, and show that intracellular corrosion is an important mechanism for early release of cobalt ions. In vitro studies show that cobalt chrome alloy particles induce the release of inflammatory mediators from macrophages before causing cell death. These mediators have significant effects on osteoblastlike cells, as well as inducing bone resorption. Variations in the cell types, implantation site, and characteristics of the particles used in experimental models make interpretation of the results difficult. Standardized methods to control for size, shape, and number of particles for testing are proposed. It is important that in vitro and in vivo findings not be taken in isolation, but be compared with the results of human studies.

Animals↗

Employment in individuals with multiple sclerosis.

The major objectives of this research were to examine unemployment in the MS population on a national level, and to identify factors which might influence an MS individual's employment status. Data used were from the National Multiple Sclerosis Survey conducted by the National Institute of Neurological and Communicative Disorders and Stroke. Data analysis was restricted to a subset of the sample who had worked at some time in their lives. Of 949 persons 79.7% were currently unemployed. While 65.2% had worked at the time of first symptom, only 27.2% were working at the time of the interview, an average of 17 years later. Path analyses revealed the overwhelming importance of mobility for remaining employed, particularly for males. Additional differences found between male and female path models were interpreted in terms of social role theory.

Disability Evaluation↗

Outcomes of patients with rheumatoid arthritis receiving rehabilitation.

PURPOSE OF REVIEW: Rehabilitation, including physical therapy and occupational therapy, complements drug therapy in the management of symptoms in patients with rheumatoid arthritis. Approximately 26% of patients with rheumatoid arthritis receive a referral for rehabilitation by rheumatologists. This review summarizes findings on the effectiveness and economic outcomes of physical therapy and occupational therapy in managing rheumatoid arthritis. RECENT FINDINGS: Studies evaluating the outcomes of various service delivery models for physical therapy and occupational therapy demonstrate improvements, especially in physical function, among people with rheumatoid arthritis. A recent pilot study examining the primary therapist model also suggests that the primary therapist model may be a viable option for delivering rheumatoid arthritis rehabilitation services. However, the evidence on other alternative models such as the physical therapy/occupational therapy practitioner model is limited. Only a few economic evaluations have been performed, and among those, none examine the cost-effectiveness of different service models. SUMMARY: Systematically interpreting the findings of service delivery models in rehabilitation is challenging because of the wide range of interventions and outcome measures used. A thorough understanding of the value of different rehabilitation models will require the guidance of a sound evaluation framework. Future clinical trials should consider including a component for evaluating cost-effectiveness. Such knowledge can contribute to evidence-informed resource allocation.

Arthritis, Rheumatoid↗

Comparison of phylogenetic metrics of transmission in symptomatic and asymptomatic tuberculosis.

BACKGROUND: Understanding drivers of Mycobacterium tuberculosis (Mtb) transmission remains a critical challenge in high-burden settings. Tuberculosis control efforts traditionally target symptomatic individuals, yet the role of asymptomatic cases in sustaining transmission is increasing recognized. METHODS: We conducted a genomic and epidemiological analysis of Mtb isolates collected in Mato Grosso do Sul, Brazil, between 2008 and 2024. From 2017 to 2022, active case finding was performed in three of the state's largest prisons, whereby sputum was collected from individuals irrespective of symptoms and tested by GeneXpert and culture. We evaluated several metrics of recent transmission from symptomatic and asymptomatic individuals, including phylogenetic clustering, Time-scaled Haplotype Density (THD), Local Branching Index (LBI), and transmission probabilities inferred using the Bayesian Reconstruction and Evolutionary Analysis of Transmission Histories (BREATH). FINDINGS: We sequenced 2,362 Mtb strains, of which 3.5% (115/2,362) were resistant to at least one drug, and 0.6% (16/2,362) were multi-drug resistant. Most strains were lineage 4, and 78.2% of all isolates were part of a genomic cluster. Among 2,362 individuals with tuberculosis, 1,137 were incarcerated at the time of diagnosis. Among these, 505 were identified through active case finding: 277 had symptomatic disease and 228 had asymptomatic tuberculosis. There was no significant difference in phylogenetic clustering proportion (77% vs. 85%; p= 0.816), THD (median 0.50 vs. 0.39; p = 0.120), or LBI (median 0.00863 vs. 0.00871; p = 0.086) between symptomatic and asymptomatic individuals. Bayesian transmission trees revealed no significant difference in the number of secondary infections inferred from symptomatic compared with asymptomatic individuals (p = 0.56). These findings were consistent across genomic clusters and robust to model assumptions. INTERPRETATION: We identified no differences in transmission from symptomatic compared with asymptomatic individuals, using several genomic measures of transmission, underscoring the substantial contribution that asymptomatic tuberculosis makes to transmission at the population level.

Asymptomatic↗

Validation and Optimization of Breeding Strategy for miR-141/200c Knockout Mice to Eliminate Off-Target Gene Silencing using FLPo Deleter.

MicroRNAs (miRNAs) of the miR-200 family specifically miR-141 and miR-200c regulate neurogenesis, differentiation, and epithelial-mesenchymal transitions in development and several diseases including cancer and stroke. The STOCK Mirc13tm1Mtm /Mmjax mouse line, which targets the miR-141/200c cluster, was originally generated and described by Park et al. 2012 as a conditional "knockout-first" allele requiring a two-step breeding strategy: FLP recombination to excise lacZ/neo cassettes followed by Cre recombination to delete the floxed miRNA cluster (1). However, subsequent studies either bypassed this step and reported knockouts based on direct crosses with Cre mouse lines, leaving residual lacZ/neo sequences that may silence upstream elements or introduce transcriptional artifacts or rare studies used less efficient FLPe Deleter mice. Here, we present a detailed and refined strategy to conditional miR-141/200c knockouts mice using FLPo Deleter mice to efficiently eliminate lacZ/neo cassettes. Our approach not only confirmed complete deletion of miR-141 and miR-200c in various organs such olfactory bulbs and lungs where these miRNAs are robustly expressed using various approach such as genotyping qPCR validation and in situ hybridization but showed that without the use of FLPo deleter mice deletion of miR-141/200c cluster amy also lead to loss of several close proximity physiologically important genes such as ptpn6, phb2, atn1 and eno1. By restoring a clean floxed allele using FLPo deleter mice prior to Cre deletion, we establish a reliable and interpretable mouse model for dissecting the roles of the miR-141/200c cluster miRNA in various disease models.

Journal Article↗

Parametric and resonant transition radiation in periodic stratified structures.

We consider the problem of electromagnetic emission when an electrically charged particle crosses a periodically stratified structure following an arbitrary linear trajectory. A theory generalizing a recently developed method [Phys. Rev. E 63, 016613 (2001)] is presented in the framework of the classical theory of electromagnetism in continuous media. It allows one to account for both the so-called parametric radiation and the resonant transition radiation. We implement our model to interpret the experiments performed by Kaplin et al. [Appl. Phys. Lett. 76, 3647 (2000)] in the x-ray domain with a stack of 300 W/B(4)C bilayers irradiated by 500 MeV electrons.

Journal Article↗

Self-interference between forward and backward propagating parts of a single acoustic plate mode.

Near and far fields of a particular (S(1)) Lamb mode, generated on a steel plate by means of a wedge transducer, are investigated. These show an oscillating behavior of the radial profile of the acoustic field amplitude that can be interpreted and modelled as interference phenomenon between forward and backward propagating parts of the Lamb mode, simultaneously generated at the interface between transducer and plate.

Journal Article↗

Thermodynamic instability in supersaturated lysozyme solutions: effect of salt and role of concentration fluctuations.

Experimental and theoretical work has suggested that protein crystal nucleation can be affected by the separation of two metastable liquid phases with different local concentrations, or more specifically by critical density fluctuations. We measure the amplitude and correlation length of local concentration fluctuations by light scattering for supersaturated solutions of hen egg-white lysozyme (at pH 4.5 and at different NaCl concentrations, up to 7% w/v). By extrapolating the critical divergent behavior of concentration fluctuation amplitude versus temperature, we determine the spinodal line, that is the limit of stability. Cloud-point measurements are used to determine liquid-liquid coexistence, consistent with previous work. In the present work, which is an extensive study of off-critical fluctuations in supersaturated protein solution, we observe a nonclassical scaling divergent behavior of the correlation length of concentration fluctuations, thus suggesting that off-critical fluctuations may have a role in crystallization kinetics. To appropriately fit the spinodal data, an entropic term must be added to the van der Waals or to the adhesive hard-sphere model. We interpret this contribution as due to the salt-induced modulation of protein hydration.

Animals↗

Real-time analysis of the telegrapher's equation for tunneling processes.

Based on a close analogy with an RLC circuit, a model for interpreting delay times in forbidden regimes (tunneling) is formulated, avoiding the analytical continuation into imaginary time. In this way, a reasonable description of experimental data, which were previously reported for a waveguide propagation below the cutoff frequency at approximately 9.5 GHz, is obtained.

Journal Article↗

Jamming during the discharge of granular matter from a silo.

In this work, we present an experimental study of the jamming that stops the free flow of grains from a silo discharging by gravity. When the outlet size is not much bigger than the beads, granular material jams the outlet of the container due to the formation of an arch. Statistical data from the number of grains fallen between consecutive jams are presented. The information that they provide can help one to understand the jamming phenomenon. As the ratio between the size of the orifice and the size of the beads is increased, the probability that an arch blocks the outlet decreases. We show here that there is a power-law divergence of the mean avalanche size for a finite critical radius. Beyond this critical radius, no jamming can occur and the flow is never stopped. The dependence of the arch formation on the shape and the material of the grains has been explored. It has been found that the material properties of the grains do not affect the arch formation probability. On the contrary, the shape of the grains deeply influences it. A simple model to interpret the results is also discussed.

Journal Article↗

Fifth-order raman spectrum of an atomic liquid: simulation and instantaneous-normal-mode calculation

Experimental artifacts and technical difficulties in carrying out theoretical calculations have consistently frustrated attempts to obtain the two-dimensional (5th-order) Raman spectrum of a liquid. We report here a new theoretical development: the first microscopic numerical simulation of the 5th-order Raman signal in a liquid. Comparison with an instantaneous-normal-mode treatment, a fully microscopic model which interprets liquid dynamics as arising from coherent harmonic modes, shows that the 5th-order spectrum reveals profound effects stemming from dynamical anharmonicity.

Journal Article↗

Observation of the alpha particle "Knock-On" neutron emission from magnetically confined DT fusion plasmas

Suprathermal fuel ions from alpha-particle knock-on collisions in fusion DT plasmas are predicted to cause a weak feature in the neutron spectrum of d+t-->alpha+n. The knock-on feature has been searched for in the neutron emission of high ( >1 MW) fusion-power plasmas produced at JET and was found using a magnetic proton recoil type neutron spectrometer of high performance. Measurement and predictions agree both in absolute amplitude and in plasma-parameter dependence, supporting the interpretation and model. Moreover, the results provide input to projecting alpha-particle diagnostics for future self-heated fusion plasmas.

Journal Article↗

Elevated CO2 Effects during Leaf Ontogeny (A New Perspective on Acclimation).

For many plants growth in elevated CO2 leads to reduced rates of photosynthesis. To examine the role that leaf ontogeny plays in the acclimation response, we monitored photosynthesis and some related parameters at short intervals throughout the ontogenetic development of tobacco (Nicotiana tabacum L.) leaves under ambient (350 [mu]L L-1)- and high (950 [mu]L L-1)-CO2 conditions. The pattern of photosynthetic rate over time was similar between the two treatments and consistent with the expected pattern for a typical dicot leaf. However, the photosynthesis pattern in high-CO2-grown tobacco was shifted temporally to an earlier maximum and subsequent senescent decline. Ribulose-1,5-biphosphate carboxylase/oxygenase activity appeared to be the main factor regulating photosynthetic rates in both treatments. Therefore, we propose a new model for interpreting the acclimation response. Lowered photosynthetic rates observed during acclimation appear to be the result of a shift in the timing of the normal photosynthetic stages of leaf ontogeny to an earlier onset of the natural decline in photosynthetic rates associated with senescence.

Journal Article↗

Fusicoccin Counteracts the n-Ethylmaleimide and Silver-Induced Stimulation of Oxygen Uptake in Egeria densa Leaves

It was previously shown that a number of sulfhydryl [SH] group reagents (N-ethylmaleimide [NEM], iodoacetate, Ag+, HgCl2, etc.) can induce a marked, transitory stimulation of O2 uptake (QO2) in Egeria densa leaves, insensitive to CN- and salicylhydroxamic acid and inhibited by diphenylene iodonium and quinacrine. The phytotoxin fusicoccin (FC) also induces a marked increase in O2 consumption in E. densa leaves, apparently independent of the recognized stimulating action on the H+-ATPase. In this investigation we compared the FC-induced increase in O2 consumption with those induced by NEM and Ag+, and we tested for a possible interaction between FC and the two SH blockers in the activation of QO2. The results show (a) the different nature of the FC- and NEM- or Ag+-induced increases of QO2; (b) that FC counteracts the NEM- (and Ag+)-induced respiratory burst; and (c) that FC strongly reduces the damaging effects on plasma membrane permeability observed in E. densa leaves treated with the two SH reagents. Two alternative models of interpretation of the action of FC, in activating a CN--sensitive respiratory pathway and in suppressing the SH blocker-induced respiratory burst, are proposed.

Journal Article↗

Gradients of Intercellular CO(2) Levels Across the Leaf Mesophyll.

Most current photosynthesis models, and interpretations of many wholeleaf CO(2) gas exchange measurements, are based on the often unstated assumption that the partial pressure of CO(2) is nearly uniform throughout the airspaces of the leaf mesophyll. Here we present measurements of CO(2) gradients across amphistomatous leaves allowed to assimilate CO(2) through only one surface, thus simulating hypostomatous leaves. We studied five species: Eucalyptus pauciflora Sieb. ex Spreng., Brassica chinensis L., Gossypium hirsutum L., Phaseolus vulgaris L., and Spinacia oleracea L. For Eucalyptus, maximum CO(2) pressure differences across the leaf mesophyll were 73 and 160 microbar when the pressures outside the lower leaf surface were 310 and 590 microbar, respectively. Using an approximate theoretical calculation, we infer that if the CO(2) had been supplied equally at both surfaces then the respective mean intercellular CO(2) pressures would have been roughly 12 and 27 microbar less than the pressures in the substomatal cavities in these cases. For ambient CO(2) pressures near 320 microbar, the average and minimum pressure differences across the mesophyll were 45 and 13 microbar. The corresponding mean intercellular CO(2) pressures would then be roughly 8 and 2 microbar less than those in the substomatal cavities. Pressure differences were generally smaller for the four agricultural species than for Eucalyptus, but they were nevertheless larger than previously reported values.

Journal Article↗