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Pasteurella multocida peritonitis in peritoneal dialysis.

Pasteurella multocida infection may occur in multiple sites to include rare involvement of the peritoneal cavity in peritoneal dialysis patients. Six cases of peritonitis associated with this organism have been reported in patients undergoing continuous peritoneal dialysis. A history of close contact with household cats was noted in all cases, with direct trauma to the dialysis tubing frequently seen. In this setting the organism appears to have a short incubation period with florid growth within 24 hours of known contamination. We report a case of Pasteurella multocida peritonitis in a patient treated with continuous cycling peritoneal dialysis and review the literature.

Animals↗

Pasteurella multocida and intrauterine device: a woman and her pets.

Human infections with Pasteurella multocida are frequently attributed to transmission from animals. Although some cases of prosthetic implant infections have been described few gynecological cases have been reported. We describe a case of intrauterine device endometritis due to P. multocida.

Adult↗

Spontaneous empyema and overwhelming septic shock due to Pasteurella multocida.

Invasive Pasteurella multocida infection, although uncommon, has been recognized to occur more frequently among patients with hepatic cirrhosis. This study reports a fatal case of bacteremic P. multocida empyema without pneumonia associated with refractory septic shock in a patient with both cirrhosis and asplenia.

Critical Illness↗

Pathophysiological changes occurring during Escherichia coli endotoxin and Pasteurella multocida challenge in piglets: relationship with cough and temperature and predicitive value for intensity of lesions.

The aims of this study were (1) to correlate cough and body temperature (BT) with the severity of bronchopneumonia in pigs, (2) to determine whether these clinical signs can be used to early diagnose bronchopneumonia and (3) to assess the predictive values of cough and BT regarding lung lesions. Bronchopneumonia was induced by administering E. coli endotoxin (LPS) combined with Pasteurella multocida type A (PmA) in the trachea of 13 piglets. Saline-instilled negative controls (n = 8), PmA inoculated (n = 6) and LPS instilled (n = 5) groups were also constituted. Cough and BT were recorded daily while the bronchopneumonia severity was assessed using bronchoalveolar lavage fluid (BALF) cytology, cytokines and measurement of lung lesion volume. Changes in expiratory breathing pattern were also measured (Penh). The combination of LPS and PmA induced a subacute bronchopneumonia characterised by macrophage, neutrophil, and lymphocyte infiltration, changes in Penh and an increase in the mRNA level of IFN-gamma while IL8, IL-18 and TNF-alpha mRNA levels remained unchanged. The daily body weight gain of infected animals was significantly reduced. Cough and BT changes were proportional to the intensity of the lung inflammatory process, functional respiratory changes and to the extent of macroscopic lesions. When comparing the individual values of cough and BT to thresholds defined for both parameters, an early diagnosis of pneumonia was possible. Considering the pooled data of each group, it was possible to define thresholds allowing an early segregation between the groups of diseased and healthy piglets. The daily values of cough and BT were predictive for the volume of lung lesions recorded at the end of the trial. In conclusion, cough and BT appear as potential indicators for the intensity and the evolution of the respiratory disease. They also seem to be good predictors for the magnitude of lung lesions and weight gain recorded at the study endpoint.

Animals↗

[Case report: a bacteremic pasteurellosis].

Bacteremic pasteurellosis is an uncommon form of Pasteurella multocida infection, usually involved in local infections. This systemic infection often occurs in immuno-compromised patient such as cirrhotic or alcoholic patients, with a high mortality rate (up to 60%). Septic shock may occur and neurological disorders or coma are frequent. We report such a case. Treatment associated local care, antibiotics (beta-lactam antibiotics plus fluoroquinolone) during 14 days and resuscitation of septic shock. Owing these therapies, septic shock was successfully treated without complications.

Aged↗

Pasteurella multocida septicemia not associated with primary liver disease.

Although systemic infections with Pasteurella multocida rarely occur in humans, liver cirrhosis associated with septicemia due to this organism has been frequently reported. Two cases of elderly women with Pasteurella multocida septicemia are described who had diabetes mellitus and breast cancer, respectively. Underlying diseases other than liver cirrhosis as well as factors hitherto unknown in otherwise healthy persons also enhance the risk of Pasteurella multocida septicemia.

Aged↗

Avian cholera on north coast California: distinctive epizootiological features.

Between 1945 and 2001, avian cholera (Pasteurella multocida infection) was confirmed at 27 epizootics in 18 different years on northcoastal California. Estimated mortality ranged from 1 to 6750 birds per site, with a median total mortality of about 1000 birds per year. Eight epizootics involved < 150 birds; thus, minor epizootics were common. Annual total wildfowl mortality ranged from 0.4% to 7.0% of estimated live populations; median annual mortality for American coots (Fulica americana) (11.5%) surpassed that of tundra swans (Cygnus columbianus) (0.2%) and ducks (0.2%). Coots comprised > 50% of total wildfowl mortality in 16 of 17 epizootics. Overall, coots comprised 82% of known avian cholera mortality, but only 34% of the live wildfowl present; ducks and swans died much less frequently. Wildfowl at one site consistently died in a sequential pattern; there was no sequential mortality at other sites.

Animals↗

Correlations of daily activity with avian cholera mortality among wildfowl.

We tested the hypothesis that wildfowl activities can influence the risk of avian cholera (Pasteurella multocida infection) for susceptible birds at Centerville, Humboldt County, California (USA). Avian cholera mortality characteristics from past epizootics were correlated with variations in flock size, habitat use and 11 feeding and nonfeeding behaviors among six empirically defined groups of wildfowl: American coots (Fulica americana), tundra swans (Cygnus columbianus), American wigeon (Anas americana), northern pintails (A. acuta), northern shovelers (A. clypeata)/mallards (A. platyrhynchos), and teal (A. discors, A. crecca, A. cyanoptera). The position of these wildfowl groups in past mortality sequences was directly correlated with mean flock size, time spent on land, and time spent grazing on land or in shallow water. We propose that variations in bird density, habitat use and frequency of grazing may serve as predisposing factors to avian cholera among wildfowl.

Animals↗

Increased susceptibility to Pasteurella haemolytica in lambs infected with bovine respiratory syncytial virus.

Groups of conventionally reared, 6- to 8-week-old lambs were inoculated intranasally and intratracheally with either bovine respiratory syncytial virus (BRSV) or BRSV followed by Pasteurella haemolytica 6 days later or with P. haemolytica alone. Lambs infected with P. haemolytica 6 days after experimental infection with BRSV had significantly higher disease scores, greater magnitudes of fever, and higher mortality rates than those infected with BRSV alone or with P. haemolytica alone (P less than 0.05). Mononuclear cells in the peripheral blood and lung lavage obtained from the BRSV-infected lambs were more susceptible to P. haemolytica cytotoxin than those obtained from control lambs (P less than 0.05). P. haemolytica was isolated in significantly greater numbers from lambs infected with BRSV and P. haemolytica than from those infected with P. haemolytica alone (P less than 0.05).

Animals↗

Pasteurella multocida septicemia caused by close contact with a domestic cat: case report and literature review.

We report here a case of Pasteurella multocida infection caused by cat exposure presenting with septic shock, sinusitis, and pneumonia. The patient was a febrile 20-year-old woman who had been experiencing disturbed consciousness progressively. She had close contact with a domestic cat and had received some scratches on both arms. A magnetic resonance imaging (MRI) scan of the head showed a high intensity in the paranasal cavity, and a computed tomographic (CT) scan of the chest showed bilateral lung consolidations. The pathogen was identified as P. multocida by the cultures from blood and nasal discharge. She was given intensive antibiotic therapy with ceftriaxone and piperacillin, continuous hemodiafiltration (CHDF) therapy, and anticoagulation therapy. Owing to these therapeutic regimens, the septic shock was successfully treated without complications. We also review the literature on P. multocida septicemia.

Adolescent↗

Toxin production by Pasteurella multocida isolated from rabbits with atrophic rhinitis.

Naturally acquired turbinate atrophy in rabbits was associated with Pasteurella multocida infection. Several in vitro and in vivo studies were conducted to document toxin production from P multocida isolates and to determine the relation of toxin to atrophic rhinitis in rabbits. Ten isolates of P multocida serotype A:12 were obtained from adult New Zealand White rabbits with noninduced atrophic rhinitis. Specific-pathogen-free rabbits inoculated intranasally with isolates of P multocida developed rhinitis and turbinate atrophy. However, inoculation with filtrates of the same bacteria failed to induce turbinate atrophy. Cytotoxicity was observed in assays, using bovine embryonic turbinate cell cultures with extracts of P multocida, but not in agar overlay cytotoxicity assays, using bovine embryonic turbinate, bovine embryonic lung, or Vero cell cultures, or in a sandwich ELISA, using monoclonal antibodies to purified P multocida toxin. Thus, turbinate atrophy was experimentally reproduced in rabbits with isolates of P multocida, but toxin was only detected in vitro by cell culture assay of P multocida extracts.

Animals↗

Pasteurella multocida in raptors: prevalence and characterization.

Several cases dealing with Pasteurella multocida infection have been documented in raptors. However, the isolates have not been fully characterized nor has the prevalence of P. multocida in raptors been determined. Three hundred ninety-eight raptors were cultured for P. multocida. Results indicated that P. multocida was not normally carried in the pharyngeal, choanal, or cloacal regions. However, P. multocida was isolated from raptors with avian cholera. Isolates from eight cases were characterized by biotype, somatic serotype, and antibiogram. Most (six of eight) of the P. multocida isolates belonged to somatic serotype 1. The remaining two P. multocida isolates belonged to somatic serotypes 3 and 3,4. The majority of the isolates belonged to the subspecies multocida. All isolates were susceptible to penicillin G, sulfisoxazole, tetracycline, and trimethoprim-sulfamethoxazole. Various restriction site heterogeneities of P. multocida chromosomal DNA were found among the raptor isolates. Results indicated that isolates of P. multocida somatic serotype 1 from diurnal raptors were genetically related.

Animals↗

Bovine herpesvirus-1 vaccination against experimental bovine herpesvirus-1 and Pasteurella haemolytica respiratory tract infection: onset of protection.

The onset of protection offered by intranasal vaccination with attenuated bovine herpesvirus-1 (BHV-1) was studied in 18 calves given a virulent BHV-1 aerosol challenge inoculum and an aerosol challenge exposure to Pasteurella haemolytica. Calves challenge exposed with virus 3, 7, 11, 15, or 19 days after vaccination and challenge exposed 4 days later with Pasteurella haemolytica did not develop viral-bacterial pneumonia, whereas 2 of 3 control calves died of fibrinous bronchopneumonia 40 and 60 hours after the bacterial aerosol and the 3rd control calf had similar lesions. All vaccinated and control calves had detectable amounts of interferon at the time of viral challenge exposure. Protection was observed before detection of neutralizing antibodies to BHV-1 in nasal secretions or in serum. Protection was therefore present from day 3 through day 19 after vaccination, but the mechanism could not be explained completely by neutralizing antibody or interferon.

Animals↗