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At least 1,009 records · Page 56Linked to original sources
PubMed1995

Cryopreservation of sheathed third-stage larvae of Gaigeria pachyscelis (Sandveld hookworm).

A technique for the cryopreservation of third stage larvae of Gaigeria pachyscelis is described. It consists of incubating sheathed third-stage larvae in 80% (v/v) ethylene glycol as a cryoprotectant for 30 s at 0 degrees C, prior to transfer into liquid nitrogen. The survival rate, as assessed by motility, was 37.6% after 30 d cryopreservation. A sheep infected percutaneously with 700 live cryopreserved third-stage larvae, harboured 41 adult worms (infectivity rate: 5.8%) when necropsied 78 d later.

Ancylostomatoidea↗
PubMed1995

An improved technique for the cryopreservation of Gaigeria pachyscelis (Sandveld hookworm).

A technique for the cryopreservation of third-stage larvae of Gaigeria pachyscelis is described. It consists of incubating sheathed infective larvae at 37 degrees C in 40% (v/v) ethylene glycol for 7 min, followed by 2 min at 0 degree C in 82.8% (v/v) cryoprotectant mixture, prior to transferring the larvae to liquid nitrogen. The survival rate obtained with this technique is consistently high: 69.1% as assessed by motility.

Ancylostomatoidea↗
PubMed1996

Wrong hookworm.

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Ancylostoma↗