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Effects of heat stress on Na+,K(+)-ATPase, Mg(2+)-activated ATPase, and Na(+)-ATPase activities of broiler chickens vital organs.

Na+,K(+)-ATPase, Mg(2+)-activated ATPase, and Na(+)-ATPase activities of brain, heart, kidney, and small and large intestinal mucosa of broiler chickens exposed to heat stress (41 degrees C, 65% relative humidity for 6 h) and thermoneutral (25 degrees C, 65% relative humidity) conditions were determined. Brain and kidneys were found to have significantly higher Na+,K(+)-ATPase activities than those of heart and intestinal mucosa. Mg(2+)-activated ATPase and Na(+)-ATPase activities in the intestinal mucosa were higher than those of brain, kidneys, and heart under thermoneutral conditions. While there was a significant inhibition of total ATPase, Na+,K(+)-ATPase, Mg(2+)-activated ATPase, and Na(+)-ATPase activity of small and large intestinal mucosa of broiler chickens exposed to heat stress, the inhibitory effect was limited to total ATPase and Na+,K(+)-ATPase enzymes in kidneys. Heat stress produced a significant increase only in Mg(2+)-activated ATPase activity of the heart, without a remarkable change in all forms of ATPase activity in the brain. Heat stress significantly decreased the ratio of Na+,K(+)-ATPase to Mg(2+)-ATPase in the heart, kidneys, and small and large intestinal mucosa. The percentage of Na(+)-ATPase in Na+,K(+)-ATPase of brain, heart, and kidneys did not significantly change during heat stress, but the ratio in small and large intestinal mucosa increased significantly during heat stress. The severe disturbances in both serum electrolytes and acid-base balance observed in previous heat stress studies could partly be mediated by direct or indirect effects of heat stress on Na+,K(+)-ATPase, Mg(2+)-activated ATPase, and Na(+)-ATPase activities of kidneys, and small and large intestinal mucosa of broiler chickens.

Adenosine Triphosphatases↗

Growth behaviour of a spore forming probiotic strain in the gastrointestinal tract of broiler chicken and piglets.

The growth behaviour of the probiotic strain Bacillus cereus var. toyoi in the gastrointestinal tract of broiler chicken and suckling piglets was evaluated. The strain germinated rapidly in intestinal samples from both animal species. Less than 10% of spores were recovered from the chicken crop and piglet stomach, respectively. Lumen samples and mucosal tissues from the hind gut of piglets displayed increasing colonization of the probiotic strain throughout the trial period. After oral administration of vegetative cells to broiler chicken and weaned piglets, sporulation was detected in all intestinal samples. The distribution of spore CFU indicated repeated germination and sporulation during the intestinal passage in piglets. B. cereus var. toyoi was not able to colonize the intestinal tract of both animal species. However, the probiotic strain was detected in suckling piglets before uptake of B. cereus var. toyoi supplemented feed. It is concluded that B. cereus var. toyoi germinates rapidly in broiler chicken and piglets, which is a necessary prerequisite for its possible probiotic effects. Germination and in vivo sporulation of vegetative cells indicated that the probiotic strain was metabolically active in the intestine of both animal species.

Administration, Oral↗

Estimates of individual factors of the tryptophan requirement based on protein and tryptophan accretion responses to increasing tryptophan supply in broiler chickens 8-21 days of age.

Ten diets (196 g CP and 13.0 MJ ME(N)/kg) with graded levels of tryptophan (Trp) between 0.9 and 2.7 g/kg were offered ad libitum to Ross broilers from day 8-21 post-hatch. Each diet was allocated to three pens of ten birds each. In addition to growth and feed conversion, the accretions of protein, Trp, fat, and energy were determined by comparative whole body analyses. A sigmoidal function was fitted to the data. Responses to Trp supply were nonlinear and attained plateau values (y(max)) within the studied range of Trp supply. The Y(max) values estimated for BW gain, whole body protein gain, and Trp gain during the 14 days under study were 615, 104, and 0.87 g/bird, respectively. Based on the response in whole body protein gain, the estimated requirement was 1.9 g Trp/kg of diet or 0.15 g Trp/MJ of MEN. Estimates made from the other response criteria were similar to this value. While the protein concentration in gained BW was unaffected by Trp intake (169 g of protein per kg of gained BW), fat and energy concentrations of gained BW increased with increasing Trp supply, approaching plateau values of 146 g fat and 9.8 MJ energy per kg of gained BW. This is supposed to result from feed intake increasing with Trp supplementation. The Trp concentration in gained whole body protein (0.84 g Trp/ 16 g of gained N) was not significantly affected by Trp intake. As long as Trp intake was the limiting factor, 59% of supplemented Trp was transferred to gained whole body protein. During the 14 days of the study, broilers inevitably lost 87 mg Trp. This is equivalent to a daily loss of 30 mg/kg BW or a maintenance requirement of 52 mg/kg BW per day. Data determined for the individual factors may be used for future modelling of broilers' Trp requirement.

Animal Feed↗

Effects of conjugated linoleic acids on growth performance, serum lysozyme activity, lymphocyte proliferation, and antibody production in broiler chicks.

This study was conducted to investigate the effect of dietary conjugated linoleic acids (CLA) on growth performance and immune responses in broiler chicks. A total of 240 day-old Arbor Acre male broiler chicks were randomly allotted into four dietary treatments with different inclusion levels of CLA (0, 2.5, 5.0 or 10.0 g/kg) for six weeks. Growth performance, peripheral blood lymphocyte (PBL) proliferation, lysozyme activity, phagocytic activity (carbon clearance) and serum antibody titers against Newcastle disease virus (NDV) vaccine were examined. There were no significant differences in growth performance among treatments (p > 0.05). Chicks fed CLA diets produced more lysozyme activity in serum than the control group at 2 and 6 weeks of age (p < 0.05). Dietary CLA enhanced the PBL proliferation in response to concanavalin A (ConA) at the age of 42 d (p < 0.05). Phagocytic ability was also affected by dietary CLA and chicks fed CLA diets had faster carbon clearance rate (p < 0.05), but antibody titers to NDV was not influenced by dietary CLA. The results of the study suggested that dietary CLA could enhance innate and cellular immune response in broiler chicks, and not affect the growth performance.

Animal Feed↗

Effect of fumonisins on macrophage immune functions and gene expression of cytokines in broilers.

Fumonisin (FB1), a mycotoxin, is produced by Fusarium moniliforme and F. proliferatum. A prevalence survey in Taiwan by our laboratory showed that there was a contamination rate of 40% in domestic animal feeds, and the average contaminated level was 4.5 mg/kg. Ninety-six birds were allotted into four treatments fed with diets containing 0 (control), 5, 10, or 15 mg/kg of FB1 for three weeks. The results showed that the growth performance was not influenced by the FB1 challenge, but relative bursa weight was significantly decreased. The activity of serum aspartate aminotransferase, and the serum levels of albumin and cholesterol were significantly elevated by the FB1 challenges. When broilers were stimulated with injection of lipopolysaccharides, mRNA abundance (determined by semi-quantitative RT-PCR) interleukin-1beta (IL-1beta), IL-2, interferon-alpha (IFN-alpha), IFN-gamma, and inducible nitric oxide synthase (iNOS) reached a plateau at 3 h, and declined at 6 h. A FB1 challenge for three weeks increased cytokine mRNA abundance in broilers. The results also showed that 15 mg FB1 per kg feed significantly inhibited the expression of IL-1beta, IL-2, IFN-alpha, IFN-gamma, but had no effect on iNOS. The macrophage functional profile was significantly changed under an exposure of 15 mg FB1 per kg for three weeks. Taken together, our results suggest that FB1 up to 15 mg/kg does not affect growth performance, but impairs some parameters of blood biochemistry and the immunocompetence in broilers.

Animal Feed↗

[Studies in male sheep on the feeding value of excreta from young hens and broilers].

5 digestibility trials were carried out, each with 4 male sheep, to investigate the excreta of young hens and broilers produced as litter in hen batteries for rearing and fattening stock. Data on the digestibility of organic matter and energy of broiler excreta were 76.0% and 76.4%; i.e. these values were only slightly lower than those for the basal ration (barley + dried green lucerne: 76.6% and 76.8%). Digestibility coefficients for organic matter and energy content in the excreta of young hens were 69.4% and 70.4%, respectively. The protein digestibility of excreta of broilers and young hens was 82.3% and 84.3%; that is to say, the investigated dropping contained 330 g and 260 g of digestible crude protein per kg of dry matter. Due to the high ash content of the samples the energy content of the analyzed excreta was only 474 EFr and 421 EFr per kg of dry matter.

Animal Feed↗

Preliminary investigation on the nutritive value of krill meal in the feed of broiler chickens and laying hens.

Krill meal was used as a protein feed in rations for broiler chickens and laying hens and its nutritional effectiveness was studied in comparison with conventional feed-stuffs of animal origin. In experiments with 650 broiler chickens from 1 to 28 and from 29 to 56 days to fattening krill meal was added to the standard feedmixture in lieu of fish meal, and dried skim-milk, and at higher levels also in lieu of soyabean oilmeal. In the experiment with 22 layer hens kept in individual cages 3% of the fish meal were substituted by 3% of krill meal. The performance of chickens fed on diets with krill meal was lower in comparison with analogical groups fed on fish meal. Higher levels of krill meal, exceeding 15 and 11% in the first and second period of feeding respectively, reduced the weight gains and feed intake of chickens. The fatty acid C14, C18 and C18:1 content of internal body fat was changed in chickens fed on higher levels of krill meal. In the experiment with hens krill meal decreased the feed efficiency, the pigmentation of egg yolks, however, was more intensive and the vitamin A content of yolks was increased in comparison with the control group. The results show that krill meal can be used as a partial substitute of fish meal in the feed of broilers and hens.

Amino Acids↗

[Effect of graded nitrogen supplements on egg protein synthesis and body protein retention in broiler hens].

In the top laying period (29th/30th week of life) egg protein synthesis and protein retention in the bodies of laying hens were ascertained. Based on egg-N analyses, the relation between N-intake/live weight0.67kg and egg-N discharge/kg LW0.67kg was regressively calculated and described. The subtraction of egg-N discharge/LW0.67kg from N-balance/LW0.67kg made it possible to estimate N-retention/LW0.67kg in the body. From the intersection of this curve with the chi-axis it follows that the broiler hen meets the N-requirement for maintenance and egg production with a daily consumption of 1,264.7 mg N/LW0.67kg, which corresponds to 15.8 g crude protein, and then neither loses nor retains body protein. At a daily intake of this amount of CP (15.8 g) the broiler hen produces 44.1 g egg per day. An energy intake of more than 70 EFUhen/animal and day resulted in fat retention in the broiler hen.

Animals↗

[Methodologic studies on the metabolism-oriented determination of methionine requirements of broiler chickens].

In a preliminary experiment the time course of 35S-excretion following a single injection of 35S-methionine in male broiler chickens was measured for 5 days. Within 2 days 65 to 69% of the amount of 35S excreted during the whole 5-day-period were recovered. Therefore, in the main experiment excrements for 35S measurement were collected only 2 days. During the main experiment starting 7 days posthatching 48 male broiler chickens received diets based on peas and wheat. The basal diet was supplemented with DL-methionine at 8 levels increasing the methionine content from 3.3 to 7.5 g/kg DM or 1,51 to 3.42 g/16 g N and the content of sulphur containing amino acids varied from 6.8 to 11.8 g/kg DM or 3.11 to 5.02 g/16 g N, respectively. Daily body weight gain was followed up to day 30 posthatching and N-balance was estimated during the 21st to 26th day posthatching. Up to day 26 a methionine content of 3.9 g/kg DM was sufficient for maximum weight gain. However, during the last period of the experiment highest weight gains were observed in animals receiving diets with 4.5 or more methionine/kg DM. Highest N-retention (1.2 g N/d) was measured if the diet contained 3.9 g methionine/kg DM or more. At day 28 posthatching 35S-methionine was injected intravenously and the subsequent 35S-excretion was measured. The 35S-excretion started to increase at a methionine content in the diet of 4.5 g/kg DM and continued to rise up to the highest methionine content of 7.5 g/kg DM. The metabolism oriented methionine requirement determination indicates for male broiler chickens at the beginning of the 5th week posthatching a methionine requirement of 3.9 to 4.5 g/kg DM (1.78 to 2.05 g/16 g N) and a requirement of S-containing amino acids of 7.4 to 8.0 g/kg DM (3.38 to 3.65 g/16 g N). The method used is potential in studying the age-dependent changes in methionine requirement.

Animal Feed↗

Effect of some antibiotics as growth promoters on performance of broiler chicks fed different protein levels.

This work was carried out to study the effect of different sources of dietary antibiotics (Zinc bacitracin, Flavomycin, Virginiamycin) in rations with optimal protein levels (starter phase: 23.5%, finisher phase: 20.0%) and low protein levels (starter phase: 19.6%, finisher phase: 17.0%) on broiler performance with 8 groups of 75 broiler each. The supplement of diets, containing the optimal and low protein level, with antibiotics increased the body weight after 7 weeks of age and improved the feed conversion as compared with control groups. Virginiamycin was superior in increasing body weights after 7 weeks of age and in improving the feed conversion with rations containing low protein level when compared with zinc bacitracin and Flavomycin. When broiler rations, low in the protein content, were supplemented with antibiotics, they could not substitute the difference in optimal protein level. Antibiotics supplementation increased the value of improvement in production efficiency and this was more pronounced with Virginiamycin.

Aminoglycosides↗

Mixture experiments: a severe test of the ability of a broiler chicken to make the right choice.

1. A mixture experiment was used to measure the biological performance (weight gain and food conversion efficiency) of broilers from 7 to 21 d of age when Fed 1 of 13 combinations of 3 protein sources. The objective was to determine the combination of ingredients that would maximise biological performance. 2. The experiment consisted of 2 diet series: in the 1st series, the 3 protein sources used were fishmeal, sunflower oilcake meal and soyabean oilcake meal, and in the 2nd, the soyabean oilcake meal was supplemented with DL-methionine. The combinations of 2 and 3-component mixtures that maximised performance in the 2 series were then compared with the selections made by chickens offered a choice of 2 or 3 components separately. 3. In all cases, the choices made by the broilers coincided with those mixtures that maximised performance. 4. It is clear from this experiment that broiler chickens will attempt to maximise performance by choosing the best possible combination of protein sources when given the opportunity to do so.

Animal Feed↗

Human factors affecting the behaviour and productivity of commercial broiler chickens.

1. The purpose of this study was to examine the relationship between stockperson attitude and behaviour; bird behaviour and productivity in the chicken meat industry. 2. No relationship between stockperson attitude and behaviour was observed, which is in contrast to studies in the pig and dairy industries. 3. Evidence is provided for the existence of sequential relationships between stockperson behaviour, bird fear levels and productivity in the broiler farms examined. 4. The mechanism(s) responsible for the negative relationships between fear levels and production is unclear. It is hypothesised that chronic stress or a series of acute stress responses may be the mechanism through which fear affects productivity. 5. The relationship between human behaviour, bird fear levels and productivity indicates the potential that exists to improve productivity and perhaps welfare of commercial broilers by reducing fear levels in broiler chickens.

Agriculture↗

Can betaine partially replace or enhance the effect of methionine by improving broiler growth and carcase characteristics?

1. Growth rates and carcase characteristics were measured in male broiler chickens fed on a control diet deficient in methionine (c. 2.8 g/kg methionine) or a series of diets containing graded levels of betaine or DL-methionine or both additives. 2. We aimed to answer 2 main questions. First, can betaine replace part of the methionine in a broiler ration? Secondly is there a synergism between methionine and betaine? 3. Birds given the control diet or that supplemented only with betaine ate less, grew more slowly, had higher food convension ratio (FCR) and varied more in mass at 42 d than birds fed diets with DL-methionine. Adding 1.2 g/kg DL-methionine to the control ration produced the heaviest birds at 42 d (2500 g) with the 2nd heaviest breast muscle (366 g). 4. After correcting for treatment differences in body mass (analysis of convariance), birds fed on the control diet and the diet supplemented with betaine only, had relatively lighter breast muscles but relatively heavier abdominal fat pads than those of birds given diets supplemented with DL-methionine. However, adding betaine to diets containing added methionine further improved the relative breast muscle yield. 5. After correcting for differences in body mass between treatments, birds fed on diets containing most methionine had lighter viscera than birds fed diets deficient in methionine. This demonstrated gut plasticity, suggesting that the viscera enlarged to sequester methionine from low-methionine diets. 6. Our data refute the hypothesis that betaine can substitute for methionine in broilers fed diets that are marginally deficient in methionine plus cystine. However, betaine may improve carcase composition, especially breast meat yield.

Adipose Tissue↗

Potential of hazelnut husks as a broiler litter material.

1. Hazelnut husks and some other litter materials were compared for litter properties and their effects on broiler performance. 2. A total of 750 1-d-old broiler chicks were weighed and assigned at random to 1 of 5 treatments, with each treatment replicated 3 times. 3. The treatments were wood shavings (control), wheat stalks, rice hulls, hazelnut husks, and a mixture of 50% rice hulls and 50% hazelnut husks. 4. Although hazelnut husks were associated with lower body weights at 49 d of age, food efficiency mortality, carcase yield, breast blisters and production index were similar to birds on wood shavings. Hazelnut husks had the highest litter moisture. 5. The results show that hazelnut husks alone and when mixed with rice hulls are a potential alternative litter material for broiler production.

Animal Husbandry↗

Influence of esterified-glucomannan on performance and organ morphology, serum biochemistry and haematology in broilers exposed to individual and combined mycotoxicosis (aflatoxin, ochratoxin and T-2 toxin).

1. A study was conducted to evaluate the individual and combined effects of aflatoxin B1 (AF), ochratoxin A (OA) and T-2 toxin (T-2) on performance, organ morphology serum biochemistry and haematology of broiler chickens and the efficacy of esterified-glucomannan (E-GM), a cell wall derivative of Saccharomyces cerevisiae1026 in their counteraction. 2. Two dietary inclusion rates of AF (0 and 0.3 mg/kg), OA (0 and 2 mg/kg), T-2 (0 and 3 mg/kg) and E-GM (0 and 1 g/kg) were tested in a 2 x 2 x 2 x 2 factorial manner on a total of 960 broiler chickens from 1 to 35 d of age in an open sided deep litter pen house. 3. Body weight and food intake were depressed by all the mycotoxins, OA being the most toxic during early life. 4. Weights of kidney and adrenals were increased by AF and OA. Liver weight was increased by AF (17.8%), while OA increased gizzard weight (14.6%) and reduced bone ash content (8.1%). T-2 toxin showed no effect on these variables. 5. Serum cholesterol content was decreased and activity of serum gamma glutamyl transferase (GGT) was increased by AF and OA while serum protein content was decreased by AF. These effects were more pronounced at 21 d than at 35 d of age. Inconsistent responses were seen in the other variables: blood urea nitrogen (BUN) content, activities of serum alanine amino transferase and aspertate amino transferase. Blood haemoglobin content was depressed by AF and T-2, whereas blood coagulation time was prolonged by OA. 6. Significant interactions were observed between any 2 toxins for their additive effects on body weight, food intake, bone ash content and serum GGT activity at 21 d. Conversely, antagonistic interactions were observed among any 2 of the toxins for their effects on variables such as serum protein and serum cholesterol content. Simultaneous feeding of all 3 mycotoxins did not show increased toxicity above that seen with any 2. 7. Esterified-glucomannan increased body weight (2.26%) and food intake (1.6%), decreased weights of liver (32.5%) and adrenals (18.9%) and activity of serum GGT (8.7%), and increased serum protein (14.7%), cholesterol (21.9%), BUN (20.8%) and blood haemoglobin (3.1%) content, indicating its possible beneficial effect on mycotoxicosis in broiler chickens.

Adrenal Glands↗

Effect of dietary Fe to Cu ratios on hematological and growth responses of broiler chickens.

A total of 2,640 broiler strain cockerels were used in three experiments to determine the interrelationships between Fe and Cu requirements of 1- to 21-day old broiler chicks. In each experiment, a starch non-fat milk basal diet was fed to chicks maintained in battery brooders beginning at 1 day of age. Maximum hemoglobin levels and packed cell volumes (PCV) of 21-day old chicks were found by feeding 80 parts per million (ppm) of dietary Fe and 8 ppm of dietary Cu from 1 to 21 days of age. The heaviest 21-day old chicks were found by feeding 80 ppm dietary Fe. Variations were found among experiments in the copper requirement based on body weight. The Cu requirement ranged from 8 to 16 ppm as indicated by 21-day old chick weights. A 10:1 dietary Fe to Cu ratio with an Fe requirement of 80 ppm appeared to maximize hemoglobin levels and PCV's of 21-day old broiler chicks; whereas, a 5:1 dietary Fe to Cu ratio, rather than a 10:1, 15:1, or 20:1 ratio, maximized 21-day old chick weights. Iron requirements for hematological and growth responses are essentially the same; however, the copper requirement may be slightly different for the two criteria.

Animals↗

Growth, metabolic and endocrine responses of broiler cockerels given a daily subcutaneous injection of natural or biosynthetic chicken growth hormone.

The effects of daily injection of natural chicken growth hormone (ncGH) or recombinant-derived chicken growth hormone (rcGH) on growth, heat production rate, plasma hormone levels and body composition were determined in rapidly growing broiler chickens. Beginning at 3 wk of age, eight broiler chickens were given a daily injection of either bicarbonate buffer (control), 100 or 200 micrograms ncGH/kg body wt, or 200 micrograms rcGH/kg body wt for 14 d. Blood samples were taken preinjection and 4 h postinjection on d 7 and 14 of chicken growth hormone (GH) treatment. Compared to preinjection levels, plasma GH levels at 4 h postinjection were significantly (P less than 0.05) elevated by daily injection (per kg body wt) of 100 micrograms ncGH (2.3-fold), 200 micrograms ncGH (5.5-fold) or 200 micrograms rcGH (6.4-fold). Although exogenous chicken GH treatment failed to increase body weight gain, ncGH injections did increase (P less than 0.05) body fat content to 117% that of the control group. Daily injection of chicken GH did not alter plasma levels of immunoreactive insulin-like growth factor-I (IGF-I), thyroid hormones, insulin, glucagon or glucose. Feed efficiency, heat production rate and respiratory quotient were also not affected by chicken GH treatment. Plasma levels of nonesterified fatty acids were elevated (P less than 0.05) by treatment with 200 micrograms ncGH/kg body wt. In contrast to domestic mammals, it is apparent that exogenous chicken GH can not be used to increase lean body mass or improve productive efficiency in chickens. Our results indicate that exogenous chicken GH exerts a strong lipogenic, rather than lipolytic, action in rapidly growing broiler cockerels.

Animals↗

Dietary carboxymethylcellulose with high instead of low viscosity reduces macronutrient digestion in broiler chickens.

The question addressed was whether the viscosity per se of dietary non-starch polysaccharides influences macronutrient digestion in broiler chickens. Water-soluble carboxymethylcellulose preparations of low (LCMC) or high viscosity (HCMC) were fed to broiler chickens (n = 10/group) from 21 to 35 d of age. The HCMC preparations reduced weight gain and raised water intake compared with LCMC. After the HCMC diet was fed, viscosity of the supernatant of small intestinal contents was significantly raised. The HCMC preparations raised the group mean ATP concentration in the digesta of duodenum plus jejunum, indicating that bacterial activity was increased. Consumption of HCMC depressed apparent fecal digestibility of lipids and nitrogen and also apparent ileal digestibility of starch. The dietary HCMC tended (P = 0.077) to reduce plasma triglyceride concentrations. After HCMC consumption, the weights of the small intestine and colon, without or with contents, were elevated. The data indicate that high viscosity of digesta in broiler chickens is associated with a reduced macronutrient digestion and impaired growth performance. Because the carboxymethylcellulose preparations were nonfermentable by fresh feces, we suggest that HCMC reduces macronutrient digestion by raising the viscosity of small intestinal contents, which is associated with enhanced bacterial fermentation due to accumulation of undigested material.

Animals↗