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A multiscale expectation-maximization semisupervised classifier suitable for badly posed image classification.

This paper deals with the problem of badly posed image classification. Although underestimated in practice, bad-posedness is likely to affect many real-world image classification tasks, where reference samples are difficult to collect (e.g., in remote sensing (RS) image mapping) and/or spatial autocorrelation is relevant. In an image classification context affected by a lack of reference samples, an original inductive learning multiscale image classifier, termed multiscale semisupervised expectation maximization (MSEM), is proposed. The rationale behind MSEM is to combine useful complementary properties of two alternative data mapping procedures recently published outside of image processing literature, namely, the multiscale modified Pappas adaptive clustering (MPAC) algorithm and the sample-based semisupervised expectation maximization (SEM) classifier. To demonstrate its potential utility, MSEM is compared against nonstandard classifiers, such as MPAC, SEM and the single-scale contextual SEM (CSEM) classifier, besides against well-known standard classifiers in two RS image classification problems featuring few reference samples and modestly useful texture information. These experiments yield weak (subjective) but numerous quantitative map quality indexes that are consistent with both theoretical considerations and qualitative evaluations by expert photointerpreters. According to these quantitative results, MSEM is competitive in terms of overall image mapping performance at the cost of a computational overhead three to six times superior to that of its most interesting rival, SEM. More in general, our experiments confirm that, even if they rely on heavy class-conditional normal distribution assumptions that may not be true in many real-world problems (e.g., in highly textured images), semisupervised classifiers based on the iterative expectation maximization Gaussian mixture model solution can be very powerful in practice when: 1) there is a lack of reference samples with respect to the problem/model complexity and 2) texture information is considered negligible (i.e., a piecewise constant image model holds).

Algorithms↗

Varying complexity in tree-structured image distribution models.

Probabilistic models of image statistics underlie many approaches in image analysis and processing. An important class of such models have variables whose dependency graph is a tree. If the hidden variables take values on a finite set, most computations with the model can be performed exactly, including the likelihood calculation, training with the EM algorithm, etc. Crouse et al. developed one such model, the hidden Markov tree (HMT). They took particular care to limit the complexity of their model. We argue that it is beneficial to allow more complex tree-structured models, describe the use of information theoretic penalties to choose the model complexity, and present experimental results to support these proposals. For these experiments, we use what we call the hierarchical image probability (HIP) model. The differences between the HIP and the HMT models include the use of multivariate Gaussians to model the distributions of local vectors of wavelet coefficients and the use of different numbers of hidden states at each resolution. We demonstrate the broad utility of image distributions by applying the HIP model to classification, synthesis, and compression, across a variety of image types, namely, electrooptical, synthetic aperture radar, and mammograms (digitized X-rays). In all cases, we compare with the HMT.

Algorithms↗

Frequency distribution and signal formation around a vessel.

We describe the NMR signal formation properties of a single vessel. Instead of assuming the frequency distribution to be a simple Lorentzian or Gaussian one, we take into account that the frequency distribution around the vessel is a complex function. Considering the static dephasing regime we find a relationship between signal formation and frequency distribution. Analytical expressions for the frequency distribution in a voxel and the magnetization decay are obtained. In the case of small volume fractions of blood and week magnetic fields the results can be used for describing signal formation processes in a vascular network. A relationship between the frequency distribution and the properties of the vascular network is derived. The magnetization decay in different time regimes is discussed. The result is relevant for describing signal formation processes around a vessel for arbitrary pulse sequences.

Animals↗

Parcel model for peak shapes in chromatography numerical verification of the temporal distortion effect to peak asymmetry.

The traditional plate concept has been reassessed and improved to a parcel matrix model, which can be used to imitate the chromatographic behavior of a hypothetic column on a computer worksheet. Under programmed conditions, various peak shapes (nearly Gaussian, and with prolonged or fronting tails) are generated. The peak tailing has been separated into two major fractions: spatial and temporal. The former fraction is caused by the retention nature of a column, whereas the latter is induced by the observer's relative position and the changing of the zone broadening rate. The temporal distortion effect can be identified qualitatively and quantitatively through a normalized peak-overlapping process. In general, a chromatographic peak may carry a prolonged (or normal type) tail under linear isotherms, while both prolonged and fronting tails will appear under non-linear conditions. The temporal distortion is proved to be significant, and may be regarded as the major cause of peak asymmetry in most cases. This is in contrast to the conclusions of many previous studies. The model is also eligible to simulate chromatographic peaks for various injection sizes.

Chromatography↗

A line-shape function in terms of changes in both molecular structure and force constants: a Gaussian approximation.

We propose a new expression of a line-shape function (LSF) including the effects of changes in both force constants and the molecular structure within the harmonic-oscillator approximation. This expression enables us to calculate the LSF using only the data on molecular structures, force constants, and electronic energies in the initial and final electronic states without solving the eigenvalue equation for the normal vibration of a molecule. To derive the LSF expression, we consider one-photon emission from a polyatomic molecule thermalized in an electronic excited state, and derive the intensity distribution function for one-photon emission using not Lax and Kubo and Toyozawa's [J. Chem. Phys. 20, 1752 (1952); Prog. Theor. Phys. 13, 160 (1955)] generating function method but rather the density-matrix method. As a simple application, a Gaussian approximate LSF is applied to SO(2). As a result, it is found that the effect of change in force constants between the initial and final electronic states cannot be ignored, nor can the effect of change in the molecular structure between these two states. The LSF expression obtained is applicable to studies of not only radiative transition but also of electron-transfer and energy-transfer processes where both changes in molecular structure and force constants between the initial and final electronic states cannot be disregarded.

Journal Article↗

Experimental validation of predicted temperature rises in tissue-mimicking materials.

Increasingly, it is recognised that diagnostic ultrasound is capable of causing temperature rises sufficient to damage tissue. Predictions of this heating are often based on simplified models of both the in vivo conditions and the relevant physical effects. Few measurements have been made to verify these predictions, however, particularly for the tightly focused beams often employed in diagnosis. Furthermore, non-linear effects in both the acoustic field and the surrounding medium have largely been ignored in calculations. To provide an alternative way to estimate the heating, NPL has developed a measurement system to determine directly the temperature rise in tissue-mimicking materials. If necessary, the measurement results can be processed to model the effects of blood perfusion. The temperature is measured using thin-film thermocouples, which have essentially no interaction with the ultrasound or thermal fields. Measurements were made on transducers operating in the frequency range 2-10 MHz and with focal beam-widths from 1 to 3 mm. The measurements agree with theoretical predictions that use either the measured beam-profile or a simplified (Gaussian) model. The results are also compared with the standard soft-tissue models developed by the National Council on Radiation Protection and Measurements (NCRP) and jointly between the American Institute of Ultrasound in Medicine and the National Electrical Manufacturers Association (AIUM/NEMA). On average, the predictions of the NCRP formula are 15% higher than the measurements, confirming its validity as a worst-case model. The predictions based on the AIUM/NEMA formula, however, are typically 30% lower than the measured values.

Body Temperature↗

Automatic grading of retinal vessel caliber.

New clinical studies suggest that narrowing of the retinal blood vessels may be an early indicator of cardiovascular diseases. One measure to quantify the severity of retinal arteriolar narrowing is the arteriolar-to-venular diameter ratio (AVR). The manual computation of AVR is a tedious process involving repeated measurements of the diameters of all arterioles and venules in the retinal images by human graders. Consistency and reproducibility are concerns. To facilitate large-scale clinical use in the general population, it is essential to have a precise, efficient and automatic system to compute this AVR. This paper describes a new approach to obtain AVR. The starting points of vessels are detected using a matched Gaussian filter. The detected vessels are traced with the help of a combined Kalman filter and Gaussian filter. A modified Gaussian model that takes into account the central light reflection of arterioles is proposed to describe the vessel profile. The width of a vessel is obtained by data fitting. Experimental results indicate a 97.1% success rate in the identification of vessel starting points, and a 99.2% success rate in the tracking of retinal vessels. The accuracy of the AVR computation is well within the acceptable range of deviation among the human graders, with a mean relative AVR error of 4.4%. The system has interested clinical research groups worldwide and will be tested in clinical studies.

Algorithms↗

Microvessel diameter estimation: error bias correction of serial measurements.

The assessment of vessel patency can be substantially improved by serial microvessel diameter measurements taken successively along an extensive length of the vessel. It is possible to avoid making the a priori assumptions about the existence or location of local constriction sites implicit in single diameter measurements. The problem then becomes one of making sense of tens or hundreds of measurements for each vessel. Equivalent diameter is defined here as as the diameter of a uniform circular cylinder of the same length as the original vessel, and having the same total resistance. Direct computation of the equivalent diameter, without taking measurement errors into account, leads to an underestimation of the true equivalent diameter even if the individual diameter measurements were not biased. We have developed a method for effectively eliminating this bias. It has been applied to serial microvessel diameter measurements of the guinea pig cochlea, automatically measured using an image analysis system. In this report, the results were developed for diameter estimates with an approximate gaussian distribution; however the method is readily extended to other error distributions. Convergence of the bias compensation was rapid. Use of the new method is advisable with as few as three diameter estimates per vessel.

Animals↗

[Luminescence properties of rare earth ions in 2SrO.0.84 P2O5.0.16 B2O3: RE3+ (RE=Ce, Tb)].

The phosphors of 2SrO.0.84 P2O5.0.16 B2O3: RE3+ (RE=Ce, Tb) were synthesized by high temperature solid state reaction. The luminescence properties of Ce3+, Tb3+ and the sensitization of Ce3+ to Tb3+ were studied. In the excitation spectrum of Ce3+, there are two broad bands at 232 and 296 nm respectively. Because of the large overlap between the emission bands, the authors could not separate them from each other. The authors could get two bands at 325 and 344 nm with the Gaussian fitting method. The possible reason is that these two peaks are from two light centers. In the phosphor of 2SrO.0.84 P2O5.0.16 B2O3: Tb3+, the excitation spectrum of Tb3+ exhibits high absorption at 370 nm and emission spectrum shows the strongest emission peak at 548 nm. The emission from the levels of (5)D3 and (5)D4 of Tb3+ appear at the same time, indicating that non-radiative process between them is inefficient. In the phosphor of Ce3+ and Tb3+ co-doped 2SrO.0.84 P2.O5.0.16 B2O3, efficient energy transfer exists.

Boron Compounds↗

Sex differences in the development of the motor activity circadian rhythm in rats under constant light.

Differences between sexes in the maturation of the motor activity rhythm have been studied in rats (10 females and 7 males) born and raised under LL and isolated from the day of weaning. Motor activity data were registered every 30 minutes for 45 days after weaning. Periodogram analysis was applied to each animal data and the daily power spectra were calculated on the basis of the period, tau. The process of rhythm evolution was studied by means of an analysis of variance of a multivariant linear model, and by defining two mathematical functions that fit to the evolution of the total daily motor activity (TDMA) and the power content of the circadian harmonic (PCCH) through time: a sigmoidal function, which presents an inflexion was used for the study of the evolution of the PCCH values through time: and a gaussian function, which presents a minimum was used for the evolution of the TDMA values. Results indicate that males show more ultradian components than females just after weaning, and take a longer time to acquire the circadian harmonic as the main harmonic in the daily power spectrum. The acquisition of the circadian character is preceded by a decrease in the TDMA, in the case of the males and is followed by a decrease in TDMA in the case of the females. These results suggest sexual differences in the organization and maturation of the circadian system in rats.

Animals↗

Deconvolution of overlapped peaks based on the exponentially modified Gaussian model in comprehensive two-dimensional gas chromatography.

Comprehensive two-dimensional gas chromatography (GC x GC) has attracted much attention for the analysis of complex samples. Even with a large peak capacity in GC x GC, peak overlapping is often met. In this paper, a new method was developed to resolve overlapped peaks based on the mass conservation and the exponentially modified Gaussian (EMG) model. Linear relationships between the calculated sigma, tau of primary peaks with the corresponding retention time (tR) were obtained, and the correlation coefficients were over 0.99. Based on such relationships, the elution profile of each compound in overlapped peaks could be simulated, even for the peak never separated on the second-dimension. The proposed method has proven to offer more accurate peak area than the general data processing method.

Chromatography, Gas↗

Extended defect related energy loss in CVD diamond revealed by spectrum imaging in a dedicated STEM.

This article aims at investigations of the low EEL region in the wide band gap system diamond. The advent of the UHV Enfina electron energy loss spectrometer combined with Digital Micrograph acquisition and processing software has made reliable detection of absorption losses below 10 eV possible. Incorporated into a dedicated STEM this instrumentation allows the acquisition of spectral information via spectrum maps (spectrum imaging) of sample areas hundreds of nanometers across, with nanometers pixel sizes, adequate spectrum statistics and 0.3 eV energy resolution, in direct correlation with microstructural features in the mapping area. We aim at discerning defect related losses at band gap energies, and discuss different routes to simultaneously process and analyse the spectra in a map. This involves extracting the zero loss peak from each spectrum and constructing ratio maps from the intensities in two energy windows, one defect related and one at a higher, crystal bandstructure dominated energy. This was applied to the residual spectrum maps and their first derivatives. Secondly, guided by theoretical EEL spectra calculations, the low loss spectra were fitted by a series of gaussian distributions. Pixel maps were constructed from amplitude ratios of gaussians, situated in the defect and the unaffected energy regime. The results demonstrate the existence of sp2-bonded carbon in the vicinity of stacking faults and partial dislocations in CVD diamond as well as additional states below conduction band, tailing deep into the band gap, at a node in a perfect dislocation. Calculated EEL spectra of shuffle dislocations give similar absorption features at 5-8 eV, and it is thought that this common feature is due to sp2-type bonding.

Journal Article↗

Combining phase and energy detection with mathematical morphology in dual time-frequency representation leads to improved SSP noise robustness.

In this paper a fusion algorithm for the detection of specular echoes blurred by coloured Gaussian noise is proposed. The phase and energy criteria, characteristic of these echoes, are handled separately. First, the detection capabilities of split spectrum (phase) and short time Fourier transform spectrogram (energy) are compared theoretically and experimentally. Second, the new algorithm based on mathematical morphology operators using both time-frequency representations is proposed. In the last part our method is shown to be more robust than absolute minimisation split spectrum processing.

Algorithms↗

Radical-molecule reactions HCO/HOC + C2H2: mechanistic study.

A detailed computational study is performed on the unknown radical-molecule reactions between HCO/HOC and acetylene (C2H2) at the CCSD(T)/6-311G(2d,p)//B3LYP/6-311G(d,p)+ZPVE, Gaussian-3//B3LYP/6-31G(d), and Gaussian-3//MP2(full)/6-31G(d) levels. For the HCO + C2H2 reaction, the most favorable pathway is direct C-addition forming the intermediate HC=CHCH=O followed by a 1,3-H-shift leading to H2C=CHC=O, which finally dissociates to the product C2H3 + CO. The overall reaction barrier is 13.8, 10.5, and 11.3 kcal/mol, respectively, at the three levels. The quasi-direct H-donation process to produce C2H3 + CO with barriers of 14.0, 14.1, and 14.1 kcal/mol is less competitive. Thus only at higher temperatures could the HCO + C2H2 reaction play a role. In contrast, the HOC + C2H2 reaction can barrierlessly generate C2H3 + CO via the quasi-direct H-donation mechanism proceeding via a prereactive complex with OH...C2 hydrogen bonding. This is suggestive of the potential importance of the HOC + C2H2 reaction in both combustion and interstellar processes. However, the direct C-addition channel is much less competitive. For both reactions, the possible formation of the intriguing interstellar molecules propadiene and propynal is also discussed. The present theoretical study represents the first attempt to probe the reaction mechanism between HOC and pi-systems. Future laboratory investigations on both reactions (particularly HOC + C2H2) are recommended.

Journal Article↗

Two-color visible/vacuum ultraviolet photoelectron imaging dynamics of Br2.

An experimental two-color photoionization dynamics study of laser-excited Br2 molecules is presented, combining pulsed visible laser excitation and tunable vacuum ultraviolet (VUV) synchrotron radiation with photoelectron imaging. The X 1Sigmag + -B 3Pi0+u transition in Br2 is excited at 527 nm corresponding predominantly to excitation of the v' = 28 vibrational level in the B 3Pi0+u state. Tunable VUV undulator radiation in the energy range of 8.40-10.15 eV is subsequently used to ionize the excited molecules to the X 2Pi32,12 state of the ion, and the ionic ground state is probed by photoelectron imaging. Similar experiments are performed using single-photon synchrotron ionization in the photon energy range of 10.75-12.50 eV without any laser excitation. Photoelectron kinetic energy distributions are extracted from the photoelectron images. In the case of two-color photoionization using resonant excitation of the intermediate B 3Pi0+u state, a broad distribution of photoelectron kinetic energies is observed, and in some cases even a bimodal distribution, which depends on the VUV photon energy. In contrast, for single-photon ionization, a single nearly Gaussian-shaped distribution is observed, which shifts to higher energy with photon energy. Simulated spectra based on Franck-Condon factors for the transitions Br2(X 1Sigmag+, v" = 0)-Br2 +(X 2Pi12,32, v+) and Br2(B 3Pi0+u, v' = 28)-Br2 +(X 2Pi12,32, v+) are generated. Comparison of these calculated spectra with the measured images suggests that the differences in the kinetic energy distributions for the two ionization processes reflect the different extensions of the vibrational wave functions in the v" = 0 electronic ground state (X 1Sigmag+) versus the electronically and vibrationally excited state (B 3Pi0+u, v' = 28).

Journal Article↗

Uncertainty analysis in matched-field geoacoustic inversions.

Quantifying uncertainty for parameter estimates obtained from matched-field geoacoustic inversions using a Bayesian approach requires estimation of the uncertainties in the data due to ambient noise as well as modeling errors. In this study, the variance parameter of the Gaussian error model, hereafter called error variance, is assumed to describe the data uncertainty. In practice, this parameter is not known a priori, and choosing a particular value is often difficult. Hence, to account for the uncertainty in error variance, several methods are introduced for implementing both the full and empirical Bayesian approaches. A full Bayesian approach that permits uncertainty of the error variance to propagate through the parameter estimation processes is a natural way of incorporating the uncertainty of error variance. Due to the large number of unknown parameters in the full Bayesian uncertainty analysis, an alternative, the empirical Bayesian approach, is developed, in which the posterior distributions of model parameters are conditioned on a point estimate of the error variance. Comparisons between the full and empirical Bayesian inferences of model parameters are presented using both synthetic and experimental data.

Journal Article↗

Establishment of paediatric biochemical reference intervals.

BACKGROUND: The standard method for the determination of reference values in a population by testing a number of healthy volunteers is difficult within the paediatric age group; this study explores an alternative approach. METHODS: Biochemical blood test results collected by the Woman's and Children's Hospital over a 1 year period were used and the data selected to ensure, as far as possible, that the results were from patients who were "healthy". This was achieved using various selection criteria, such as the exclusion of patients with more than one test episode. Some of the data were skewed, making standard statistical approaches difficult. In such cases, transformations were used to ensure that the resulting information had a distribution that was approximately Gaussian. RESULTS: Data from different biochemical tests covering both genders and ages in the range 0-18 years were collected; this included more than 250 000 laboratory test results. After elimination of non-representative data and tests with insufficient results, there were approximately 23 500 results covering almost 3000 individuals and 16 different biochemical tests. There were no results from very young children (< 1 year) after the data selection process; hence the derivation of reference values from that age group was not possible. CONCLUSIONS: This approach permitted better delineation of reference intervals for common biochemical tests performed on paediatric patients than is currently readily available. In addition, some important benchmarks for transformation of medical data have been found.

Adolescent↗

Factors raising intracellular calcium increase red blood cell heterogeneity in density and critical osmolality.

BACKGROUND: In normal human blood, RBC volume (V), surface area (A) and hemoglobin content (H) exhibit Gaussian distributions with coefficients of variation (CV) of 10-15%. Strikingly narrower distributions are observed for their cell density (CV approximately 0.5%) and filterability (CV approximately 5-7%). This implies that V is highly correlated with H and A. We hypothesize that the RBC is able to adjust its volume to parameters H and A. It is tempting to speculate that intracellular free calcium (Cai) is a mediator in this process, acting as an activator of the Gardos channel. We tested this hypothesis by experimentally varying Cai and measuring changes in RBC density and filterability distributions. MATERIAL/METHODS: Three different approaches were used to raise Cai: (i) RBCs were loaded with Ca2+ in the presence of the calcium ionophore A23187; (ii) RBCs were incubated with Ca2+ in the presence of 1.0 mM ortho-vanadate; and (iii) the calcium pump was switched off by ATP depletion of RBCs. The density distribution of RBCs was determined by a phthalate technique. The distributions in filterability were obtained using a kinetic filtrometer. RESULTS: Whatever the approach used, the density and filterability distributions of treated RBCs broadened significantly in comparison with those of control cells. CONCLUSIONS: The results obtained suggest that (i) in vivo regulation of RBC volume is mediated by Cai and (ii) Cai probably depends on the A/V ratio, which determines the stress experienced by the RBC membrane in the circulation and, thereby, the calcium influx rate.

Adenosine Triphosphate↗