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PubMed1975

The microbiological and parasitological colonisation of specified-pathogen-free mice maintained in a conventional animal house.

2 groups of 44 specified-pathogen-free (SPF) mice, caged in groups of 4, were housed at weaning either in isolation in a building housing conventionally-reared animals, or in contact with those animals. 4 mice from each group were examined for microbiological flora and 4 for parasites at intervals for up to 90 days after transfer. Proscribed bacteria were isolated from the "in contact' group after 42 days and endoparasites were observed by 16 days and thereafter. No proscribed bacteria were isolated from the other group, although some endoparasites were observed by 16 days. Proscribed bacteria were shown to be present in the conventionally-reared animals at weaning and ecto- and endoparasites by day 16 and thereafter. It is suggested that it may be possible to maintain SPF animals outside a SPF Unit for short periods of time, and that with great care the animals remain free of proscribed microflora.

Animal Feed↗
PubMed1985

Dynamics of the urinary tract in longterm vesico-ureteral reflux with infravesical obstruction and infection in pigs; V.

In a study of the dynamic function of the urinary tract in infravesically obstructed pigs with vesicoureteric reflux, 5 pigs with 6 refluxing ureters evidenced urinary tract infection after 8 and 10 weeks of obstruction. Examination showed that the median ureteric baseline activity had decreased to 0.0 A.P./min (action potentials/min). During bladder filling, no increase in activity was seen, although there was pressure equilibration between the bladder and the renal pelvis, when the reflux producing bladder pressure was reached. Only a few episodes of retrograde activity were recorded during the bladder filling. 46% of the ureteric contractions were incomplete and stopped in the mid-third of ureter. The study showed the severe, irreversible impairment of ureteric function when subjected to not only reflux and obstruction but also infection.

Animals↗