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An improved polystyrene polymeric XAD-2 resin column extraction of 5beta-cholestan-3beta-ol from polluted water.

Appropriate methods and criteria for judging the degree of sewage contamination of water and its suitability for drinking or recreational uses are essential to safeguard the public health. 5beta-cholestan-3beta-ol seems to satisfy many, if not all, of the criteria required of a good indicator of fecal pollution. It was shown that this fecal sterol was strongly adsorbed to the polystyrene polymeric XAD-2 adsorbents at low pH, resulting in a 100% retention. The adsorbed sterols could be easily removed from the columns with acetone adjusted to pH 8.5-9 with concentrated NH4OH. It has also been demonstrated that large volumes of both fresh and sea water samples can be extracted by the "closed" column method in a relatively short time. The sensitivity of the column exceeded that of the conventional liquid-liquid partitioning procedure. With some modifications, the column extraction process can be incorporated in a fully- or semi-automated analytical procedure.

Cholestanols↗

Headspace sampling and gas chromatographic determination of styrene migration from food-contact polystyrene cups into beverages and food simulants.

Migration studies using coffee, tea, water, and 8% ethanol were conducted with various types of food-contact polystyrene cups. Study conditions simulated filing and storage at room temperature and hot-filling or pasteurization above 150 degrees F (65.6 degrees C). The quantity of styrene migrating was determined by headspace sampling and gas chromatography (GC). The GC column was 6% Carbowax 20M on Chromosorb 101 with quantitation via a flame ionization detector. Detection limits ranged from 3 to 10 ppb in various liquids. The method and results of the migration studies are presented.

Beverages↗

Early adhesion of bacteremic strains of Staphylococcus epidermidis to polystyrene: influence of hydrophobicity, slime production, plasma, albumin, fibrinogen, and fibronectin.

Twenty bacteremic strains of Staphylococcus epidermidis were characterized according to their hydrophobicity, their ability to produce slime, and their in vitro adhesion to polystyrene microtiter plates precoated or not with plasma proteins. Four strains of Staphylococcus aureus were also tested for adhesion. Slime production in S. epidermidis was not correlated with initial adhesion, whether measured qualitatively or by a quantitative method. Hydrophobicity (xylene:water partition) was well correlated with adhesion. Slime production, adhesion, and hydrophobicity were highly strain dependent among S. epidermidis organisms. For S. epidermidis, early adhesion was inhibited (10% to 98%) by albumin and fibronectin in all strains, by plasma (19 strains), and by fibrinogen (18 strains). Stimulation occurred for one strain with plasma and two strains with fibrinogen. In contrast, adhesion was inhibited by albumin and markedly stimulated (twofold to 14-fold) by plasma, fibrinogen, and fibronectin for the four strains of S. aureus. Early adhesion of S. epidermidis to polymer surface appears to depend mainly on hydrophobicity and is usually impaired by plasma proteins, albumin, fibrinogen and fibronectin; with a heterogeneous behavior among the different strains tested. Slime production would interpose secondarily, after the first attachment.

Bacterial Adhesion↗

Polystyrene tube immunoradiometric assay for human alpha1-fetoprotein, and its use for mass screening.

We describe a two-site immunoradiometric assay for human alpha1-fetoprotein, with use of antibody-coated polystyrene tubes as solid phase. The sensitivity, precision, and simplicity of this system make it eminently suitable for mass-screening purposes. We currently use it for neonatal detection of hereditary tyrosinemia in the Province of Quebec; measurements are made on blood spotted and dried on paper. This system could be well suited for other mass surveys, such as prenatal screenings for fetal abnormalities.

Amino Acid Metabolism, Inborn Errors↗