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At least 1,027 records · Page 57Linked to original sources
PubMed1984

Salt and base sequence specific changes in the chiroptical properties of DNA.

Chiroptical properties of natural DNA molecules differing in base composition were studied in solutions with high concentrations of monovalent sodium and caesium salts. It was found that the properties were dependent on the DNA base sequence and nature of both cations and anions. A comparison with the behaviour of the synthetic molecules of DNA demonstrated that the salt-induced changes in the natural molecules of DNA could not be accounted for by the appearance of the left-handed Z conformation. On the other hand, the tendency of the alternating A--T sequence to assume the novel X--DNA conformation seems to play a role even in the conformational properties of natural DNA.

Animals↗
PubMed1984

Comparison of bone levels after intramuscular administration of cephradine ('Velosef') or flucloxacillin/ampicillin in hip replacement.

Serum and bone levels of antibiotics were measured after the intramuscular injection of either 1 g cephradine or 500 mg flucloxacillin plus 500 mg ampicillin in 48 patients undergoing total hip replacement. The results showed that both regimens produced adequate blood levels, but that the levels after cephradine were higher and more prolonged. Bone levels of cephradine were significantly higher than for flucloxacillin or ampicillin. In half of the patients studied, flucloxacillin bone levels were below the limit of detection in both cancellous and cortical bone samples. In all 24 patients, cortical levels of cephradine were satisfactory for anti-staphylococcal activity although cancellous levels were unsatisfactory in 4 patients. The penetration of the antibiotics was greater into cortical bone than into cancellous bone.

Aged↗
PubMed1978

Improved microbiological assay for penicillin residues in tissues and stability of residues under cooking procedures.

The microbiological assay for penicillin residues was improved by using centrifugation to remove physical barriers to diffusion, a small buffer/meat extraction ratio, and a more sensitive 2-layer assay system. Recoveries from muscle, kidney, and liver tissues ranged between 70.1 and 86.7% with measurable levels of 0.03--0.05 unit/g. By comparison, the Food and Drug-suggested methodology yielded recoveries of 45.9--54.0% and levels of detectability of 0.08--0.10 unit/g. Cooking of hamburger, steaks, and port chops indicated that procaine penicillin withstood cooking conditions, and significant levels of the original activity remained.

Animals↗
PubMed1978

[Analysis of the germ content of the air in university lecture rooms (author's transl)].

The microflora of the air in university lecture rooms was examined with the sedimentation method. For this purpose blood agar (20 degrees C and 37 degrees C), saline agar and Endo plates were used. The germ counts identified correlate to the size and utilization of the rooms. The lowest germ count was determined in the room used for practical work, which was followed by the lecture room. The newly built lecture room contained the highest colony count. The floor ventilation furnishes an explanation for this. Most of the colonies were Gram-positive cocci (micrococci, indifferent streptococci, sarcins). Aerobic spore-forming organisms were found to be present in smaller quantities than cocci. Fungi, mainly, moulds grew better at a temperature of 20 centigrade. The microbiological monitoring of air forms an important part of environmental protection. Great importance can be attached to a stardization of the air germ count in the prevention of airborne infections.

Air Microbiology↗
PubMed1981

[New trends in the treatment of trophic sequelae post-phlebitic syndrome].

Authors analyses most recent concepts on pathogenesis of trophic changes in post-phlebitic syndrome: particularly they consider the role of extravascular fibrin deposition, in patients with depressed plasmatic fibrinolytic activity. In this patients, according to recent reports, it seems useful a fibrinolytic therapy to improve trophic conditions of the post-phlebitic limb, particularly in respect to dermatoliposclerosis. Authors refer their experience on a double blind study with stanozolol an anabolizing steroid with fibrinolytic activity: in all treated patients good clinical results were obtained; however no enhancement of plasmatic fibrinolytic activity was demonstrated.

Adult↗
PubMed1981

Correlation between fluorimetric and microbiological methods for determination of cephalexin in urine and serum.

The fluorimetric and microbiological methods for the determination of serum and urine cephalexin were compared with the aid of coded samples in two different laboratories. The coefficient of correlation between the two methods for serum cephalexin was 0.94. At urine cephalexin concentrations above 0.5 g/l, the coefficient of correlations between two methods was 0.83. At lower concentrations (0.5 g/l), the coefficient of correlation was 0.72. It was concluded that the tested methods are comparable, equally sensitive and precise.

Biological Assay↗
PubMed1981

[Kinetic and clinical study of bacampicillin].

A mean plasma concentration curve, with peaks within the first hour and moderate individual variations, was obtained after a single administration of 800 mg of bacampicillin to 5 healthy volunteers. An adequate antibiotic transfer from the circulation to the bronchial apparatus was demonstrated. Treatment of 34 patients suffering from ampicillin-sensitive bacterial diseases permitted clinical cure of all patients, without onset of side-effects. It is concluded that bacampicillin is preferable to oral ampicillin because of its kinetic and tolerance features.

Adolescent↗
PubMed1980

[Epidemiological evaluation of thromboembolic risk. Critical review of the prospects offered by determination of antithrombin III].

Authors review the epidemiology of deep venous thrombosis and the prothrombotic state in high risk patients; particularly antithrombin III role in blood clotting is considered. A fall in antithrombin III concentration and/or activity can induce hypercoagulability and thrombosis. Moreover authors stress the need to evaluate antithrombin III as anti-Xa activity, to detect high risk patients for deep venous thrombosis.

Antithrombin III↗
PubMed1980

[Internal abdominal hernias. Presentation of 10 treated cases].

Ten cases of abdominal internal hernia observed at the Niguarda Hospital Emergency Surgery Division, Milan, during the period 1969-1978 are described. Even though it is extremely uncommon, this form must be kept in mind in the course of differential diagnosis when the picture includes: typical and striking symptoms of ileal strangulation, radiographical evidence of marked distension of the ileal loops with hydro-air levels, and a negative history of abdominal surgery.

Adult↗