A simplified method for mapping deletion/substitution mutants of bacteriophage lambda.
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The N gene product induces lambda-delayed early mRNA synthesis. This transcription was sensitive to chloramphenicol at the very early stage of phage development, but became resistant within 2-4 min after infection. However, the same transcription was sensitive to the antibiotic throughout phage development if lambda tof- mutant was used as the infecting phage. The results suggest involvement of the tof gene in lambda early transcription; the tof product may act as a stabilizer of the N gene function.
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