PubMed Health⌕ Search

SEARCH · PubMed Health

Results for “Transfer”

Explore indexed PubMed citations for clinical trials, systematic reviews and public health research. Read source abstracts and follow each citation to its original PubMed record.

Quote a phrase for an exact phrase match. Source license links do not imply unrestricted reuse.

At least 1,045 records · Page 58Linked to original sources
PubMed1999

[Transfer of injured patients. Analysis of injured patients transferred to the Casualty Hospital in Brno from other hospitals in 1997].

The author analyzes a group of casualties referred in 1997 to the Casualty Hospital Brno from other institutions where the patients were hospitalized after the injury, if the period did not exceed 30 days. Thus 361 casualties were referred. The reasons are summarized. Problematic is according to the author the admission of 37 patients with multiple injuries and 30 patients with a single injury. During the contemplated reduction of the number of health departments incl. surgical ones in the Czech Republic it will be difficult to justify the further existence of departments which are unable to cope permanently with uncomplicated bone fractures.

Adolescent↗
PubMed1975

The structural basis for the resistance of Escherichia coli formylmethionyl transfer ribonucleic acid to cleavage by Escherichia coli peptidyl transfer ribonucleic acid hydrolase.

Escherichia coli formylmethionly-tRNA-tMet is unique among N-acylaminoacyl-tRNAs in its resistance to cleavage by peptidyl-tRNA hydrolase. Chemical modification of tRNA-fMet with sodium bisulfite converts fMet-tRNA-fMet into a good substrate for the hydrolase. The products of the enzymatic cleavage are free tRNA-fMet and formylmethionine. Bisulfite treatment produces cytidine to uridine base changes at several sites in the tRNA structure. One of these modifications results in formation of a new hydrogen-bonded base pair at the end of the acceptor stem of tRNA-fMet. We have shown that this modification is responsible for the observed change in biological activity. Enzymatic cleavage appears to be facilitated by the presence of a 5-terminal phosphate at the end of a fully base-paired acceptor stem, because removal of the 5-phosphate group from N-acetylphenylalanyl-tRNA-Phe or bisulfite-modified fMet-tRNA-FMet reduced the rate of hydrolysis of these substrates. The unpaired base at the 5 terminus of unmodified fMet-tRNA-fMet appears to reduce susceptibility of the tRNA to hydrolytic attack both by positioning the 5-phosphate in an unfavorable orientation and by directly interfering with enzymatic binding. The unusual structure of the acceptor stem of this E. coli tRNA thus plays a critical role in maintaining the viability of the organism by preventing enzymatic cleavage of the fMet group from the bacterial initiator tRNA.

Amino Acyl-tRNA Synthetases↗
PubMed1979

Total synthesis of a tyrosine suppressor transfer RNA gene. XVII. Transcription, in vitro, of the synthetic gene and processing of the primary transcript to transfer RNA.

Primer- and promoter-dependent transcription of the synthesis gene had been studied. Primer-dependent transcription gave, as a major product, an end-to-end transcript which was strand-specific. The transcript was characterized rigorously by two-dimensional separation and analysis of the oligonucleotides formed on digestion with T1-RNase and pancreatic RNase and by nearest neighbor analyses of the oligonucleotides obtained when different alpha-32P-labeled ribonucleoside triphosphates were used as substrates. Minor products accompanying the major transcript were characterized similarly. The major transcript, when treated with an Escherichia coli S-100 extract, was processed to the tRNATyr with correct 5'- and 3'-ends. The nucleolytic cleavages occurring at the 3'-end were characterized. In promoter-dependent transcription, transcription of a restriction fragment containing phi80psu+III gene and the synthetic gene with and without the promoter were compared. Transcription of the synthetic gene was promoter-dependent and strand-specific, the initiation of transcription occurring at the same point as previously found in vivo. Although the synthetic gene contains only 16 base pairs corresponding to the natural sequence following the C-C-A end, processing of the transcript at the 3'-end occurred normally, the endonucleolytic cleavage being followed by exonucleolytic cleavages. The products of promoter-dependent transcription were completely characterized. An examination of the base modifications of the primary transcript during treatment of the latter with E. coli S-100 extract showed couplete modification of uridine to pseudouridine and partial methylation of uridine to ribosylthymine in TpsiCG sequence and partial formation of pseudouridine in the anticodon loop. However, hardly any formation of 2'-O-methylguanosine or of 2-methylthio-6-isopentenyl adenosine could be detected.

Base Sequence↗
PubMed1975

Two photo-cross-linked complexes of isoleucine specific transfer ribonucleic acid with aminoacyl transfer ribonucleic acid synthetases.

Escherichia coli Ile-tRNA synthetase and tRNA-Ile have been cross-linked photochemically by the direct action of ultraviolet light. In addition, photo-induced joining of tRNA-Ile E. coli to Val-tRNA synthetase from yeast has also been achieved. This yeast enzyme is known to mischarge E. coli tRNA-Ile with valine. Regions on tRNA-Ile involved in cross-linking have been determined for both complexes. In each case, three distinct parts of the nucleic acid are found cross-linked. Two of these are the same in both complexes and involve the dihydrouridine stem and loop region. The third part is unique for each complex. It involves the 3' terminus in the cognate one and the 3' side of the anticodon in the non-cognate case. When the cross-linked regions are projected onto a model of the three-dimensional structure of tRNA, it is clear that these and other data are consistent with having each enzyme bound in a similar orientation across tRNA-Ile. The enzymes are viewed as spanning the distance from the anticodon to the 3' terminus and making extensive contact with the area in which the two helical branches of the L-shape tRNA structure come together.

Amino Acyl-tRNA Synthetases↗
PubMed2003

[Information transfer between health structures. How and to whom is the information transferred?].

The evolution of the French health-care system is based on the development of patient management in the health-care system. The public services associate nurses with alternatives to hospitalization in order to favor the emergence of such facilities. This evolution changes the habits and the needs of professionals. Communication between the nurses is the basis of this function. In order to assure the continuity of health-care, both private and hospital nurses need information. However this is either not the case, or is inadequate to the expectations and needs of the nurses. The motives will be analyzed and some proposals offered. In recording and analyzing liaison records, interesting accounts were revealed which foresees the possibilities of an evolution at the information network level between the hospital and the extra-hospital sector. Some propositions will be put forth in order to assure a greater coherence in the endeavor for health-care continuity.

Attitude of Health Personnel↗