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Random amplified polymorphic DNA markers for DNA fingerprinting and genetic variability assessment of minute parasitic wasp species (Hymenoptera: Mymaridae and Trichogrammatidae) used in biological control programs of phytophagous insects.

Biological control of insects that feed on our crops has become more practical in recent years by mass release of egg parasitoid microhymenoptera. Trichogramma species are now commercially reared and spread in commercial fields to control specific insect pests. Microhymenoptera species are, however, very small and morphologically indistinguishable within species, although strains of a given species differ in their efficiency to control specific insect pests. Traditional taxonomy is unable to differentiate microhymenoptera species at the strain level. It is becoming increasingly important to develop a reliable system to monitor genetic variations both within and between strains of commercially important microhymenoptera, to detect genetic drift occurring during several generations of multiplication, to protect patents, and to certify the lots of commercially released microhymenoptera. We have developed a system based on DNA markers to rapidly characterize individuals of five species of microhymenoptera from the genus Anaphes and Trichogramma including a new species of Anaphes not previously described. The main components of our system are a rapid and simple DNA micro-extraction method and fast DNA polymorphism analyses based on random amplified polymorphic DNA markers.

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Systematics, evolution, and biology of scelionid and platygastrid wasps.

The Platygastroidea comprises two families of parasitoids, Scelionidae and Platygastridae, and nearly 4500 described species. They parasitize a diverse array of insects as well as spiders. Idiobiont endoparasitism of eggs is the putative ground plan biology, as reflected by all scelionids, but most Platygastridae are koinobiont endoparasitoids of immature Auchenorrhyncha, Sternorrhyncha, and Cecidomyiidae. The superfamily is demonstrably monophyletic but its phylogenetic position remains uncertain. Relationships within the Platygastroidea are also poorly known and the group is in need of comprehensive phylogenetic study. Significant information is available on host relationships and biology, although much of this is biased to a few genera of Telenominae that are employed as biocontrol agents. Hosts for many genera are unknown, in particular those that inhabit leaf litter or parasitize solitary host eggs. The Trissolcus basalis-Nezara viridula parasitoid-host association has become a favored model system in ecological, behavioral, and physiological research on insects.

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Parasitoidism of Chalcidid wasps (Hymenoptera, Chalcididae) on Philornis sp. (Diptera, Muscidae).

Philornis Meinert larvae are known as parasites of birds, with coprophagous, semi-hematophagous or hematophagous habits. Biological data of the larvae of the fifty described species are still scarcely known. Here we describe some aspects of the parasitism of a species of Philornis on Thalurania glaucopis Gmelin (Trochilidae) and record two species of Chalcididae (Hymenoptera) parasitoids, Conura annulifera (Walker, 1864) and Brachymeria podagrica (Fabricius, 1787), reared from Philornis puparia.

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Parasitization by pteromalid wasps (Hymenoptera) of freeze-killed house fly (Diptera: Muscidae) puparia at varying depths in media.

Three laboratory experiments were performed to assess parasitization of freeze-killed house fly puparia, buried 0 to 6 cm in media, by Muscidifurax raptor Girault & Saunders, Muscidifurax raptorellus Kogan & Legner, Muscidifurax zaraptor Kogan & Legner, Trichomalopsis sarcophagae (Gahan) and Urolepis rufipes (Ashmead) (Hymenoptera: Pteromalidae). Virtually no parasitization occurred at depths greater than 1 cm in large arenas (988 cm2) with densities of 0.3 puparia and 0.008 female parasitoids per cm2. Parasitization was observed at depths as great as 4 cm for three of five species in small arenas (3 cm2) with densities of 6.4 puparia and 1.0 female per cm2. Combined across experiments, M. raptor achieved the highest level of parasitization, followed by M. zaraptor, M. raptorellus, U. rufipes, and T. sarcophagae. The greatest number of F1 females was produced by the gregarious species T. sarcophagae (834 female female) and M. raptorellus (708 female female), and then by the solitary species M. raptor (530 female female), M. zaraptor (365 female female) and U. rufipes (163 female female). High parasitization by M. raptor and high production of offspring by T. sarcophagae identify these species as being particularly attractive as biological control agents.

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Mastoparan, a wasp venom peptide, stimulates release of prolactin from cultured rat anterior pituitary cells.

Studies have shown that mastoparan and other amphiphilic peptides induce exocytosis of hormones from anterior pituitary cells. We have studied the effect of mastoparan on the secretion of prolactin from cultured rat anterior pituitary cells and on the concomitant functional status of signal-transducing pathways in lactotroph-enriched cell cultures. Mastoparan stimulation of prolactin secretion was dose-dependent, time-dependent, reversible and required the presence of calcium. Pretreatment of pituitary cell cultures with cholera and pertussis toxin had no effect on the secretory response, whereas encapsulation of guanosine 5-[beta-thio]diphosphate (GDP-beta-S) by reversible electropermeabilization inhibited mastoparan-stimulated secretion. Incubation of mastoparan with myo-[3H]inositol-labelled lactotroph-enriched anterior pituitary cell cultures resulted in increased formation of inositol phosphates compared with control cells, and encapsulation of GDP-beta-S blocked mastoparan-induced inositol lipid hydrolysis. Mastoparan caused translocation of protein kinase C activity from a soluble to a membrane-attached form. Mastoparan was able to increase the intracellular Ca2+ concentration in Fura-2-loaded individual lactotrophs. Omission of Ca2+ from the extracellular medium did not change the Ca2+ response in lactotrophs when stimulated with mastoparan. On the basis of these results it is concluded that mastoparan-induced release of prolactin is preceded by activation of the inositol(1,4,5)trisphosphate/diacylglycerol pathway with resulting translocation of protein kinase activity and increment in intracellular Ca2+. However, other signal-transducing pathways may be involved in the secretory process.

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