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Alcohol intake of P rats is regulated by muscarinic receptors in the pedunculopontine nucleus and VTA.

Experiments were conducted to determine whether muscarinic receptors within the pedunculopontine nucleus (PPN) and ventral tegmental area (VTA) are involved in regulating ethanol drinking behavior in the alcohol-preferring P line of rats. Female P rats were given limited access (2 h/day) to 10% (v/v) ethanol and 0.0125% (g/100 ml) saccharin solutions. Food was available ad libitum. Cholinergic agents were microinjected unilaterally into the PPN or VTA immediately prior to ethanol access. Intra-PPN carbachol (1-4 microg/0.5 microl), which can inhibit cholinergic neuronal activity within the PPN, decreased ethanol (70% decrease at the highest dose; p < 0.05) and saccharin (90% decrease at the highest dose; p < 0.05) intake in a dose-dependent manner within the first 30 min. Intra-PPN scopolamine (5-15 microg/0.5 microl), which can stimulate cholinergic neuronal activity within the PPN, decreased ethanol intake in a dose-dependent manner within the first 30 min (65% decrease at the highest dose; p < 0.05) without reducing saccharin intake. Intra-VTA methylscopolamine (1-10 microg/0.5 microl), a muscarinic antagonist, significantly (p < 0.05) reduced ethanol (60% decrease at the highest dose) and saccharin (50% decrease at the highest dose) intakes during the 2-h access period. Intra-VTA carbachol, a cholinergic agonist (1 and 2 microg/0.5 microl) decreased ethanol consumption in a dose-dependent manner within the first 60 min (50% decrease at the highest dose) without reducing saccharin intake. Overall, these results support an involvement of the cholinergic PPN-VTA system in regulating alcohol drinking and general consummatory behaviors of the P line of rats.

Alcohol Drinking↗

Personal expectancies and group influences affect alcoholic beverage selection: the interaction of personal and situational variables.

Eighty-five college student drinkers participated in a study designed to investigate competing expectancies in predicting drinking in each of three situations. Participants were assigned to either a solitary, social-information (others aware of drink selected), or social-no-information (others present but unaware of beverage selection) condition, and asked to watch a TV show which they would later be asked to rate. As part of the procedure, they were told that the beverage of their choice (selected from a list provided by the experimenter) would be made available to them. Results indicate that when no information is available to others concerning beverage selection, the expectancy that alcohol enhances social and physical pleasure is the sole predictor of beverage selection. When others are likely to know what one has selected, fear of negative evaluation is the sole predictor of selection. Results are discussed in terms of the importance of evaluating multiple reinforcers and expectancies in attempting to predict drinking behavior.

Adult↗

Studies of whole blood associated acetaldehyde as a marker for alcohol intake in mice.

Thirty C57BI mice were randomized into two groups. Group 1 served as controls while Group 2 was given 10% V/V ethanol with the drinking water. Whole blood- associated acetaldehyde (WBAA) was measured on capillary blood samples using a fluorigenic high performance chromatographic assay. WBAA peaked at Day 2. A stable mean plateau of 263 +/- 71 SD with a range of 160-400 nmoles/g hemoglobin WBAA was found in the group consuming ethanol compared with 122 +/- 17 SD and a range of 88-150 nmoles/g hemoglobin for controls (p less than 0.001). When ethanol was discontinued, levels of WBAA declined and became similar to those of controls by 9 days following cessation of ethanol. The quantitative difference between ethanol-consuming and control animals and also the rapid rise of whole blood-associated acetaldehyde and the relatively slow decline following cessation of ethanol intake indicate that such a test might be a useful monitor of drinking behavior.

Acetaldehyde↗

University students' knowledge of alcoholic drinks and their perception of alcohol-related harm.

A total of 371 university students were asked to estimate the amount of alcohol contained in a standard drink and to estimate the number of standard drinks contained in popular alcoholic beverages. In addition, students completed questionnaires assessing their perception of short and long term harm related to the consumption of beer, wine, spirits and pre-mixed alcopops. Results revealed that students were generally inaccurate in their estimate of alcoholic content of beverages, and national guidelines for low risk drinking. Students were also found to hold different perceptions regarding how harmful different alcoholic beverages were. While both male and female students considered spirits to be more harmful than beer, wine and pre-mixed drinks, males also believed beer to be more harmful than wine when considering the short term consequences. The pattern of beliefs reported by this sample suggest a high-risk population who are not aware of the risks they are exposing themselves to through their drinking behavior.

Adult↗

Ingestive behaviors of rats with electrolytic median raphe lesions maintained on a food deprivation schedule.

The current study examines the effects of electrolytic median raphe lesions on the feeding and drinking behaviors of rats maintained on a 23-hr food deprivation schedule. Measurement of food intake during the feeding period indicated no difference between the two groups but lesioned animals showed significantly more spillage than controls. Furthermore, measurement of water intakes indicated that median raphe lesioned rats consume significantly less water than controls in the absence of food and come to ingest virtually all of their daily fluid intake during the feeding period. Additionally, rats with lesions of this nucleus take significantly more drinks during the feeding period, often punctuating their meal with short draughts. Since similar behaviors are seen following the destruction of certain hypothalamic and limbic nuclei anatomically related to the median raphe, it is possible that these syndromes share an anatomical basis.

Animals↗

Unilateral cortical spreading depression in the rat: effects on feeding, drinking and other behaviors.

The effects of unilateral cortical spreading depression (CSD) on feeding, drinking and other behaviors were studied in rats. CSD waves were elicited by a microinjection of KC1 solution through an implanted cannula. The animal's behavior and slow potential changes (SPC) accompanying CSD were then simultaneously monitored for 20 min. Intensive face grooming was observed while the CSD wave-front propagated across the sensorimotor cortex. Animals commenced their first feeding or drinking shortly after the invasion of the negative SPC into the frontal cortex of Krieg's area 6 and 10 [14] had reached its maximum height. A two-way switching effect between the first feeding or drinking and quiescence (freezing) and a close relationship between their onsets were observed. The results are discussed specifically in relation to cortico-hypothalamic pathways.

Animals↗

Adverse health behaviors and the detection of partner violence by clinicians.

BACKGROUND: Intimate partner violence (IPV) is a major public health problem in the United States, and victims are commonly encountered in medical settings. Many barriers exist to clinician-initiated screening for IPV. However, smoking and problem drinking are conditions that clinicians commonly screen for and both have been strongly associated with IPV in prior studies. By estimating the predicted probability of 12-month and lifetime IPV for a given patient based on whether she presents with these conditions, our study gives clinicians information that can help them identify patients at risk for IPV. METHODS: A cross-sectional written patient survey was administered to 2386 female patients at 8 different health care settings in the Greater Boston (Mass) metropolitan area. The probabilities of 12-month and lifetime IPV were estimated based on the women's self-report of smoking and drinking behaviors. RESULTS: A woman who neither smoked nor engaged in problem drinking had a 10% probability of IPV in the preceding 12 months and a 39% chance of IPV in her lifetime. Smoking increased the probability to 14% and 49%, respectively. Problem drinking resulted in a doubling of the predicted probability of 12-month IPV to 21%, with a lifetime probability of 43%. When both conditions were present, the effects were additive, with a woman having a 27% probability of experiencing IPV in the preceding 12 months and 54% chance of IPV in her lifetime. CONCLUSIONS: The presence of smoking or problem drinking should raise clinicians' suspicion for IPV. This paradigm should not replace direct questioning about IPV but may aid in the detection of abuse in patient populations.

Adolescent↗

Risk and protective factors for alcohol use among pregnant African-American, Hispanic, and white adolescents: the influence of peers, sexual partners, family members, and mentors.

Factors affecting the drinking patterns of 183 pregnant, African-American, Hispanic, and White adolescents were investigated, including the influence of peers, sexual partners, family members, and mentors. Among participants who drank alcohol prior to their pregnancies, 87% quit or cut back on their drinking behaviors. Those adolescents who lived in environments where their partners, peers, and relatives were drinking and using drugs were more likely to have consumed alcohol in the previous month, while adolescents who identified mentors and/or parents who provided high levels of support were less likely to have consumed alcohol. Finally, distinct patterns of both drinking and life circumstances among the African-American, Hispanic, White adolescents were revealed. The implications of these findings for future research and intervention with pregnant adolescents are discussed.

Adolescent↗

Drinking for wages: alcohol use among cantineras.

OBJECTIVE: This study reports on patterns of alcohol use among cantineras: recently immigrated Latina women who make a living drinking in working class Latino bars (cantinas). METHOD: Women working in 10 cantinas in a Latino neighborhood participated in a semistructured interview that included questions about their personal background and current working conditions. Twenty-one participants were interviewed at their home or place of work. RESULTS: Drinking is a duty required of women who work in cantinas, and the conditions in which alcohol is consumed varies by the type of job they perform. Cantineras establish drinking habits and routines to maximize profits and maintain their jobs, while adopting coping strategies to minimize adverse health consequences. CONCLUSIONS: The drinking behavior of cantineras presents a paradox. To make a living, they need to become efficient drinkers who profit from their behavior. At the same time, they need to recognize and set limits to their drinking. The norms of cantineras distinguish drinking as duty from drinking for recreation, and they recognize problem drinking. The heavy health burden imposed by obligatory drinking at work is the result of limited job opportunities and the marginality experienced by Latina immigrants who must find ways to cope with the pressures they endure as poor women.

Adaptation, Psychological↗

Adults' approval and adolescents' alcohol use.

PURPOSE: To compare adults' approval of adolescents' alcohol use among white, black, and Latino youth and to evaluate the effects of approval on most recent alcohol consumption, past 30-day use and binge drinking. METHODS: A cross-sectional telephone survey of N = 6245 adolescents from 242 communities was conducted as part of the National Evaluation of the Enforcing Underage Drinking Laws Program. The survey assessed perceived availability of alcohol, underage alcohol use, and problems related to underage drinking. Ordinary least squares regression modeling was used to test the relationships between adults' approval and most recent consumption. Logistic regression modeling was used to measure the association among approval, past 30-day use and binge drinking. RESULTS: Perceived consequences, parent and adult relative provision of alcohol, and drinking with a parent were protective of underage drinking. Providing alcohol at a party, however, was associated with a two-fold increase in past 30-day use and binge drinking. There were minimal differences on adults' approval across the three racial/ethnic groups. CONCLUSIONS: Adults' approval of alcohol use is highly correlated with youth drinking behavior and has differential effects on adolescents' alcohol use depending on the social context in which the alcohol is provided.

Adolescent↗

Central nervous system depression associated with glucocorticoid ingestion in a dog.

Signs of a central nervous system disorder were observed following 2 instances of accidental ingestion of glucocorticoid in a young female Doberman Pinscher. The signs included transient aggressive and paranoid behavior, amaurosis, disorientation, ataxia with circling backward, and depression. Vomiting, weight loss, and abnormal drinking behavior persisted for several weeks following recovery from the acute illness.

Animals↗

Disturbance of motivated behavior in rats by epileptic afterdischarges.

Nearly all epileptic seizures in patients are characterized by deranged consciousness. We started to study changes in motivated behavior (drinking in thirsty rats) as a possible analogue of compromised consciousness during and after epileptic seizures. Epileptic afterdischarges (ADs) were elicited by stimulation of the dorsal hippocampus and/or thalamus. Rats with implanted electrodes (deprived of water for 24 hours) were trained to lick water from a narrow tube. After pretraining ADs were elicited eight times in each animal and access to water was allowed during different phases of the AD. Stimulation did not affect licking if no AD was induced. If stimulation was successful, licking was stopped in nearly 70 % of stimulations and modified (biting the tube) in 30 %. Hippocampal ADs (characterized by serrated waves in the EEG and by an arrest of behavior with subsequent automatisms) completely blocked licking, signs of recovery appeared during the interval between the AD and recurrent AD and it progressed during recurrent ADs. Thalamic ADs abolished licking in 82% of cases and immediately after ADs normal licking reappeared in 49 % of these observations. Our results suggest that changes in motivated behavior might serve as an analogue of compromised human consciousness.

Animals↗

Characterization of central actions of neuropeptide Y on food and water intake in rabbits.

The effects of a 36-amino acid peptide, neuropeptide Y (NPY), on feeding and drinking behaviors were studied in young and adult rabbits. Intraventricular injection of NPY to adult rabbits induced feeding and drinking in a dose-related manner. While the lowest doses tested (0.2 micrograms) was without effect, other doses (0.5 and 1 microgram) elicited feeding and drinking almost instantaneously. When 1, 5 and 10 microgram doses were injected into young rabbits, immediate increases in feeding and drinking were evident, but differences in the magnitude of responses among these dosages were significant only in water consumption. Unlike studies in rats, in these rabbits NPY elicited a more pronounced response in drinking than in feeding. The drinking response after NPY administration was not a consequence of food intake because it occurred in the absence of food. With ad lib feeding, the majority of enhanced food consumption was confined to the first 30-min after NPY injection; however, an increased motivation to eat was retained for at least 2 hr after NPY when food was withheld and then returned. These observations are consistent with specific stimulatory effects of NPY on food and water intake.

Age Factors↗

Drinking patterns among Hispanic adolescents: results from a national household survey.

OBJECTIVE: This is one of the first studies to examine and compare alcohol use for adolescent Cubans, Mexican Americans, Puerto Ricans and other Hispanics. METHOD: The data come from the 1993 National Household Survey on Drug Abuse (NHSDA), a national probability sample of the U.S. household population. The sample examined here (N = 1,865, 52% male) comprises 200 Cubans, 1,133 Mexican Americans, 255 Puerto Ricans and 277 Central/South Americans who were 12 to 17 years old. Drinking patterns are measured using a quantity-frequency index, and analyses are conducted using Stata. RESULTS: In the cross-tabulations, no ethnic differences in drinking patterns are found for males or females, nor is there evidence of gender differences within ethnic groups, although there are some age differences in alcohol use. In the logistic regression analyses, two ethnic differences emerge, although the factors most consistently associated with drinking behaviors in these analyses are age, Spanish language use and urban residence. Additional analyses using the 1998 NHSDA suggest that ethnic differences in alcohol use may emerge in late adolescence/early adulthood. CONCLUSIONS: Given the established findings of ethnic and gender differences in drinking among adult Hispanics, it is surprising that few differences are evidenced in adolescence. Future research should explore whether such differences emerge during the transition into adulthood and, if so, identify factors that produce them. In addition, to increase understanding of these ethnic groups' drinking patterns, future research should further investigate the factors associated with Hispanic adolescents' alcohol use, including both consideration of whether the predictors are the same across groups and of the role of sociocultural factors.

Acculturation↗

Alcohol-consumption behaviors and risk for physical fighting and injuries among adolescent drinkers.

This study examined the associations between specific alcohol-use measures and physical fighting, injuries received, and injuries inflicted on others while fighting. We conducted cross-sectional analyses of the National Longitudinal Study of Adolescent Health (Add Health) limiting our analyses to adolescent drinkers (n=8885) between the ages of 12 and 21 years. Results revealed that adolescent drinkers who reported problem drinking and peer drinking were more likely to engage in physical fighting, being injured, and injuring others in fights than drinkers who did not report these drinking behaviors even after controlling for drinking frequency and binge drinking. The findings highlight the need for violence prevention programs that focus on the reduction of alcohol use among adolescents.

Adolescent↗

[Determination of the development of psychotic dependence to drugs in small animals. 2. Selective drinking of morphine and cocaine solutions and its reinforcement in mice].

To develop a screening method for psychic dependence liability of the drugs, morphine- and cocaine-directed drinking behavior was studied in mice. Using automatic drinkometer the intake of drug solution from the paired sucking tube connected to tap water and/or drug solution was estimated. The drinking pattern showed a diurnal rhythm and large portion of the total intake was done during dark period. When the animals were forcedly given drugs as drinking solution total intake and the gain of body weight was suppressed in proportion to the concentration of the drugs. In the choice test between tap water and drug solution, mice can distinguish the concentration of drug solution and avoid it in high concentrations. Animals experienced drug solution before the choice test showed a tendency to intake more drug solution than the untreated control animals depending on the length of pretreatment. Pretreatment of the animals with twice daily injections of the drugs failed to develop high grade of preference to drug solution in the succeeding choice test. Thus, the positive reinforcing properties of morphine and cocaine was demonstrated in mice and the validity of this animal species for the screening of the drugs of psychic dependence was suggested.

Animals↗

Naturalistic observations of beer drinking among college students.

We observed the beer drinking behavior of 308 university students in several bar and party settings. The following relationships were found: males drinking beer in bars consumed 0.92 oz per min; females drank less beer than males, and stayed in a bar for a longer time period; patrons drank significantly more beer when drinking in groups and when purchasing beer in pitchers versus cups or bottles; and intervals between party arrival and first drink and between party departure and last drink varied inversely with blood alcohol concentration. We discuss these findings with regard to developing interventions to prevent alcohol-impaired driving.

Adolescent↗

Older women's cognitive and affective response to moderate drinking.

OBJECTIVE: In this paper we investigated the question, how do older women who drink moderate amounts of alcohol differ from those who do not drink on measurements of cognitive function, memory, affect and health? METHODS: The nonprobability sample of female participants (n=182) averaged 75 years of age and had a Mini Mental State Examination scores of 28. The participants were asked to indicate whether they drank alcohol or abstained (yes/no) and if they indicated that they did drink, to describe how many drinks they consumed in a given period of time (day/week/month). RESULTS: None of the participants acknowledged drinking more than 2 drinks a day. Caucasian women had the largest number of moderate drinkers (53% vs 47%), while the majority of African-American and Hispanic women reported not drinking. The moderate drinkers reported less depression, had higher self-reported health, performed better on instrumental everyday tasks, had stronger memory self-efficacy, and used more strategies to improve memory performance. In addition, these women had higher performance on tests of executive function: attention, concentration, psychomotor skills, verbal-associative capacities, and oral fluency. CONCLUSIONS: The circumstances under which people drink are complex and were not evaluated in this study. Therefore, rather than endorsing drinking behavior, these findings suggest that future research might examine why elders make the decision to drink, the circumstances that predispose women to drink (alone/with others), and other qualities that characterize female drinkers over the age of 65.

Activities of Daily Living↗