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At least 1,063 records · Page 59Linked to original sources
PubMed1983

Temporary intraarticular knee stabilization with synthetic material (Polyester-Silicon) in the sheep.

We draw the following conclusions from these animal experiments: a) It is possible to stabilize the knee joint of a sheep after resection of the anterior cruciate ligament by a suitable synthetic band. A foreign body reaction did not occur. b) After replacement of the anterior cruciate by an auto-, homo- or heterologous material, it was not necessary to immobilize the joint. c) In comparison to a control group, it appears that the transformation of the replacement material occurs faster due to the functional stimulation under guided mobility. d) The smooth surface of the synthetic material prevents its incorporation into connective tissue. Therefore, it is possible to remove this synthetic material after sufficient strength of the implanted material has been obtained. e) By fixing the synthetic band on the femoral shaft and the tibial head it can be removed without problems by small skin incisions without opening the joint. f) Kinking, wear and fatigue of the synthetic material can be avoided by rerouting the band around the lateral femoral condyle and the tibial edge. g) These results of animal experiments cannot be transferred to the human knee without great caution. Yet, it seems at least technically possible to achieve primary knee stability by a synthetic band in connection with a suture or replacement of the anterior cruciate.

Animals↗
PubMed1993

Glycoconjugate mediated endothelial cell adhesion to Dacron polyester film.

PURPOSE: The purpose of this study was to explore new strategies for enhancing specific cell type attachment to biomaterials using immobilized lectins for cell surface glycoconjugates. The lectin Ulex europaeus I (UEA I) has a high affinity for human vascular endothelial cell surface glycoconjugates. METHODS: UEA I was covalently bound to polyethylene terephthalate (Dacron) with the cross-linking agent 1-ethyl-3-(dimethylaminopropyl)carbodiimide hydrochloride to achieve oligosaccharide-mediated endothelial cell attachment to this otherwise nonadherent surface. RESULTS: Experiments with radiolabeled UEA I demonstrated covalent linkage of as much as 1.35 micrograms/cm2. The lectin binding site is available after the reaction, as demonstrated in experiments a neoglycoprotein. Adhesion studies reveal a 100-fold increase in endothelial cell attachment for the UEA I/polyethylene terephthalate surface (99.7 +/- 29.6 cells/high-power field) when compared with untreated (0.7 +/- 0.5), crosslinking agent (0.4 +/- 0.3), and denatured UEA I (1.2 +/- 1.1) control groups. Five vascular endothelial cell lines adhered to the UEA I/polyethylene terephthalate surface, whereas monocytes, smooth muscle cells, and fibroblasts did not. CONCLUSION: These results imply new strategies for endothelialization of prosthetic grafts and promotion of selective cell adherence to biomaterials, with emphasis on carbohydrate interactions. Moreover, this experimental system offers a model for exploring the biologic significance of the endothelial cell-UEA I ligand.

Cell Adhesion↗