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PubMed1996

Discrimination performance and resolution capacity of uncrossed visual pathways in one-eyed albino rats.

Our recent study has demonstrated that adult rats with one eye removed at birth (OEB) relearn a discrimination between alternating black and white stripes, 30 mm wide each, oriented horizontally and vertically faster than control rats monocularly enucleated in adulthood (OET), when relearning is carried out after lesions of the visual cortex and transections of the optic tract contralateral to the remaining eye. Yet, we could not obtain the corresponding results in the acquisition of the discrimination following the same surgical treatments: OEBs did master the discrimination, whereas OETs did not. We hypothesized that a large discrepancy in OETs' performance between the acquisition and relearning occurred because the stripes were close to the limit of the resolution capacity of the uncrossed visual pathways (UXVPs), and hence that a better performance of OEBs was to indicate an increase in the resolution capacity, which resulted from reorganization of the UXVPs brought about by monocular enucleation at birth. To test the hypothesis we tried to approximate the limit of the resolution capacity of the UXVPs in OEBs and OETs using seven different sized test stripes ranging from 20 to 5 mm in width after both OEBs and OETs had relearned the discrimination of the 30 mm training stripes following the same surgical treatments mentioned above. It was found that the median width of the smallest stripes for OETs to discriminate was 10 mm, and that of OEBs 7.5 mm. Although OETs could not discriminate the smallest stripes which OEBs could, they were able to discriminate stripes one third smaller than those hypothesized. Based on these findings the possibility was discussed that the acquisition as well as the relearning of the discrimination of the 30 mm stripes mediated by the UXVPs in OEBs and OETs might not be influenced by the resolution capacity, but mostly, if not entirely, by the size of the visual field.

Animals↗

PubMed1987

Vision beyond the resolution limit: aliasing in the periphery.

Pattern resolution is generally considered a prerequisite for spatial vision because details too fine to be resolved cannot be distinguished from a uniform field. However, our experiments using peripheral vision demonstrate that reliable pattern detection is possible for images far beyond the resolution limit. The visual percept which arises in this case is an illusion called aliasing in which the apparent spatial structure of the stimulus is quite different from that actually present. Aliasing begins at spatial frequencies just above the classical resolution limit, which is taken as evidence that peripheral resolution is limited by the coarse spacing of visual neurons rather than by increased size of their receptive fields. At a given eccentricity, the very finest pattern which produces aliasing has a spatial period which approaches the smallest anatomical dimension: the diameter of a single cone photoreceptor.

Form Perception↗

PubMed1991

Visual resolution limits in human albinism.

The effects of the involuntary ocular oscillations on visual resolution was examined in 22 albinos and 11 idiopaths with congenital nystagmus. The idiopaths showed a linear relationship between the proportion of the slow phase spent at low velocities (less than or equal to deg/sec) and the log of the minimum angle of resolution; such that long dwell times were compatible with good resolution. For the albinos there appeared to be a critical duration of low retinal slip velocities above which there was no improvement in acuity. This supports the contention that factors other than the congenital nystagmus limit visual resolution in the albino.

Albinism, Ocular↗

PubMed1992

Effects of lateral masking and spatial precueing on gap-resolution in central and peripheral vision.

The data made available by this experiment add two points to the information obtained by previous studies on eccentric vision and lateral masking. First, they demonstrate a clear effect of target-mask similarity on visual gap-resolution in eccentric vision: increasing target-mask similarity systematically decreases resolution performance. Second, spatial precueing has no effect on gap-resolution performance. One implication of this experiment is that with the present stimulus configurations, visual resolution should decrease much more strongly with eccentricity than it does with classical isolated or bar-masked optotypes.

Humans↗

PubMed1994

Genetic recombination in E. coli: RuvC protein cleaves Holliday junctions at resolution hotspots in vitro.

The E. coli RuvC protein resolves Holliday junctions during genetic recombination and postreplication repair. Using recombination intermediates made by RecA protein, we have identified specific "hotspots" for RuvC resolution. Characterization of these sites reveals a common tetranucleotide sequence, with the consensus 5'-A/TTT decreases G/C-3'. The correct orientation of the resolution site is required for cleavage. These observations suggest that the strand bias of this sequence will affect the outcome of recombinational crosses by directing resolution to either "patch" or "splice" recombinant products. Mutation of the consensus site in synthetic Holliday junctions abolishes or significantly reduces the efficiency of cleavage, although binding is unaffected, demonstrating that junction recognition and incision are biochemically separable events. We propose that efficient RuvC resolution requires the translocation of Holliday junctions to specific cleavage sites, thus providing a biochemical basis for the similar genetic defects observed in ruvA, ruvB, and ruvC mutants.

Bacterial Proteins↗

PubMed1984

System for DNA sequencing with resolution of up to 600 base pairs.

A system capable of resolving about 500 bases is of interest for sequencing of longer DNA molecules. Studies on further optimization of resolution on DNA sequencing gels were carried out. The effect of physico-chemical properties of gels and buffers on resolution were tested, e.g. ionic strength and pH of buffers, different buffer systems, acrylamide concentration, crosslinker concentration, type of crosslinker, temperature of polymerization, denaturing conditions, gel length and thickness. Tested were as well different running conditions like electric field, gel temperature, dimension of sample slots. Gels 0.1-0.2 mm thick and up to 1.2 m long were cast and tested routinely. Gel lengths of 60-70 cm (for sequencing up to 350-400 bases) to about 100 cm (above 400 bases) are practicable. Little is gained in resolution by increasing the gel length from 1 to 1.2 m. Resolution was improved using 0.1 mm thick gels, at a higher pH value of 8.6-8.8, and molarity increased to 0.2 M. The sequencing pattern in the region of higher bases could be better resolved on a twice-magnified picture of that region on the autoradiogram. With the long gels (70-120 cm), it is advantageous to obtain the sequence overlap by running in parallel gels of different concentrations, without re-application of samples, all loaded at the same time. Buffer chamber for running of two of three gels and thermostating plates up to 1.2 m long were designed. In this way four to six thermostated gels can be run from a power supply with two inputs. Three 1 m long gels (concentrations: 4%, 6%, 12-16%) are loaded with several samples of DNA to be sequenced and run in parallel without re-application of the samples. With good samples, the sequence overlap from the gels could be counted up to 500 base pairs, with exceptionally good samples closer to 600 bases. At present this number seems to be near the limit of the resolving power of the polyacrylamide gels.

Base Sequence↗

PubMed1978

The timing of CNV resolution in a memory retrieval task.

Twelve men performed the Sternberg memory retrieval task while the EEG and EOG were recorded. Subjects saw a target set of 1 to 4 digits followed by a warning tone that was followed after 1.5 sec by a probe digit. Subjects indicated by pressing one of two levers whether the probe digit was in or out of the target set. The timing of contingent negative variation (CNV) resolution was measured as the latency of 50% resolution in stimulus-synchronized waveforms (SSWs), response-synchronized waveforms (RSWs), and model waveforms (MWs). The MWs were constructed by adding each RSW to itself using time displacements derived from the reaction times of the single trials of which the RSW was an average. The results indicated that CNV resolution is related to the timing of the motor response. MWs had resolution latencies close to those of the SSWs. In SSWs the N1 and P3 to the warning tone also varied with target set size.

Adolescent↗

PubMedDate not supplied

Spatial and temporal resolution of aortic Doppler blood velocity signals in normal human subjects.

We have used digital, microcomputer-based methods for the numerical analysis of pulsed, range-gated, ultrasonic Doppler blood velocity signals recorded with an axially aimed transducer from the ascending aorta of normal subjects. We investigated the spatial, temporal and amplitude resolution of our methods for recording and analysis, comparing aortic signals with test signals from a sine wave function generator. The spatial resolution of our system was found to be adequate in spite of the use of a relatively large sample volume. The temporal resolution was significantly influenced by the rate of change of velocity which affected the results of discrete fast Fourier transform analysis. The rate of increase in aortic blood velocity at the start of ejection was linear, following an initial jump in velocity. The amplitude resolution was affected by fluctuations in the amplitude of the Doppler signals. We conclude that our methods do not exaggerate the bluntness of the aortic velocity profile or the linearity of the increase in blood velocity at the start of left ventricular ejection.

Adolescent↗

PubMed1992

Image contrast in high-resolution electron microscopy of biological macromolecules: TMV in ice.

It is shown that the contrast in high-resolution electron micrographs of biological macromolecules, illustrated by a study of TMV in ice, falls considerably below the level which should theoretically be attained. The factors which contribute to the low contrast include radiation damage, inelastic scattering, specimen movement and charging. Future progress depends on improved understanding of their contributions and relative importance. Contrast is defined as the amplitude of a particular Fourier component extracted from an image in comparison to that expected by extrapolation from separate electron or X-ray diffraction measurements. The fall in contrast gets worse with increased resolution and is particularly serious at 10 A and beyond for specimens embedded in vitreous ice, a method of specimen preparation which is otherwise particularly desirable because of the expectation that the embedded molecules should be well preserved in a near-native environment. This low contrast at high resolution is the principal limitation to atomic-resolution structure determination by electron microscopy. In spite of good progress in the direction of better images, it remains a major problem which prevents electron microscopy from becoming a simple and rapid method for biological atomic structure determination.

Bacteriorhodopsins↗

PubMed1996

Functional MR imaging of visual and motor cortex stimulation at high temporal resolution using a FLASH technique on a standard 1.5 Tesla scanner.

Functional magnetic resonance imaging (fMRI) was performed on a conventional 1.5 T scanner by means of a modified FLASH-technique at temporal resolutions of 80 and 320 ms. The method's stability was assessed by phantom measurements and by investigation of three volunteers resulting in a low amplitude (3%) periodic (4 s) signal modulation for the in vivo measurements, which was not observable in the phantom experiments. fMRI activation studies of motor and visual cortices of four adjacent slices were carried out on 12 healthy right-handed volunteers. Stimulation was performed by a triggered single white light flash or single finger-to-thumb opposition movement, respectively. Event-related response of visual and motor activation was traced over 10.24 s with a temporal resolution of 320 ms for the four slice measurements. Brain activation maps were calculated by correlation of measured signal time course with a time-shifted boxcar function. Activation was quantified by calculation of percentual signal change in relation to the baseline. Observed signal magnitudes were about 5-7% in visual and about 8-12% in primary motor cortex. While photic response was delayed by about 2 s, motor stimulation showed an instantaneous increase of the MR signal. MR signal responses for both stimuli had decayed completely after about 5 s. Our results show that event-related fMRI enables mapping of brain function at sufficient spatial resolution with a temporal resolution of up to 80 ms on a conventional scanner.

Adult↗

PubMed1989

Human enamel structure studied by high resolution electron microscopy.

Human enamel structural features are characterized by high resolution electron microscopy. The human enamel consists of polycrystals with a structure similar to Ca10(PO4)6(OH)2. This article describes the structural features of human enamel crystal at atomic and nanometer level. Besides the structural description, a great number of high resolution images are included. Research into the carious process in human enamel is very important for human beings. This article firstly describes the initiation of caries in enamel crystal at atomic and unit-cell level and secondly describes the further steps of caries with structural and chemical demineralization. The demineralization in fact, is the origin of caries in human enamel. The remineralization of carious areas in human enamel has drawn more and more attention as its potential application is realized. This process has been revealed by high resolution electron microscopy in detail in this article. On the other hand, the radiation effects on the structure of human enamel are also characterized by high resolution electron microscopy. In order to reveal this phenomenon clearly, a great number of electron micrographs have been shown, and a physical mechanism is proposed.

Dental Caries↗

PubMed1990

Cold stage design for high resolution electron microscopy of biological materials.

Both the number and range of applications of cryotechniques in transmission electron microscopy are increasing rapidly. In some cases, most notably the determination of protein structure by electron crystallography, progress has been limited by the performance of commercially available cryo stages. We review the design and performance criteria for stages which will be necessary for wide applicability in high resolution studies of biological specimens. The important criteria include an operating temperature below -140 degrees C with a low rate of contamination of the specimen, ability to tilt to 60 degrees, and perhaps most important, good resolution as judged by an effective modulation transfer function of 0.8 at 0.35 nm. Most applications also require an effective cryotransfer system. Up until now, most work in high resolution electron crystallography has been accomplished with laboratory-built stages which meet some, but not all, of these criteria. The availability of cold stages which fully meet criteria will allow the rapid expansion of high resolution studies by electron microscopy in structural biology.

Cryopreservation↗

PubMed1990

Imaging of protein molecules--towards atomic resolution.

This review discusses some of the recent developments in high resolution imaging of biological molecules. Electron micrographs of unstained biological molecules never show the resolution or contrast that would be predicted. Movements in the specimen caused by radiation damage, and possibly charging of the specimen are the most significant factors in the reduction of image contrast of these radiation-sensitive specimens. Until these limitations are overcome it is unlikely that the structures of biological molecules will be determined to the resolutions to which they are preserved. The causes of contrast loss in images are discussed in a quantitative manner and the use of crystalline paraffin as a model for radiation-sensitive specimens in general is described. Procedures for improving the contrast in images of biological molecules are described, including the new method of spot-scan imaging. Possible future developments, including high resolution imaging of single particles, are discussed.

Microscopy, Electron↗

PubMedDate not supplied

Matching pulmonary structure and perfusion via combined dynamic multislice CT and thin-slice high-resolution CT.

Taking advantage of two scan modes of an electron beam CT scanner (Imatron), we have developed a method utilizing x-ray CT for relating pulmonary perfusion to global and regional anatomy. A high temporal resolution mode, used to follow bolus contrast agent, is combined with a high spatial resolution mode to obtain the structure-function fusion. A software module has been developed for our image analysis package (VIDA) to automatically calculate physiologic parameters of flow and integrate these color coded functional measurements into a corresponding high spatial resolution data set. We present the scanning methodology details and give examples from our physiologic based research to demonstrate strengths of combining dynamic and high resolution CT to uniquely characterize pulmonary normal and pathophysiology.

Algorithms↗

PubMed1996

Morphometric analysis of noninvasively assessed bone biopsies: comparison of high-resolution computed tomography and histologic sections.

This article describes a new method to analyze the structural behavior of trabecular bone structure by means of noninvasive measurements of the three-dimensional cancellous bone architecture. For the noninvasive data acquisition, a high-resolution quantitative computed tomography system for peripheral measuring sites (pQCT) was used. With this system and the help of a multiple thin-slice measuring technique, it became possible to examine three-dimensional bone structure with a resolution of 0.25 mm. Using a special three-dimensional segmentation algorithm, mineralized bone was separated from bone marrow and muscle tissue within the three-dimensional stack of CT slices. These segmented data sets can then be processed nondestructively and, even more important, repetitively in either two or three dimensions. In order to validate this noninvasive procedure, a two-dimensional comparative morphometric study was performed including CT slices and corresponding histologic sections prepared after CT measurement. Three representative sections from the three-dimensional stack of CT slices were selected and the morphometric indices of the segmented CT slices were compared with the indices stemming from the corresponding histologic sections prepared after CT measurement. Although the presented approach can only give an example of the method, the results from the morphometric analysis support the assumption that cancellous bone structures based on noninvasive high-resolution CT measurements are representative for trabecular microstructures assessed from histologic bone sections. The study demonstrates the potential of high-resolution CT imaging for in vivo applications of quantitative bone morphometry. This is especially true for repetitive follow-up measurements, which cannot be performed using histologic sections. Additionally, the method offers an easy access to the three-dimensional structure of trabecular bone, which is mandatory for the analyses of the anisotropic mechanical behavior of cancellous bone.

Algorithms↗

PubMed2004

Application of multivariate resolution methods to the study of biochemical and biophysical processes.

Multivariate resolution methods make up a set of mathematical tools that may be applied to the analysis and interpretation of spectroscopic data recorded when monitoring a physical or chemical process with multichannel detectors. The goal of resolution methods is the recovery of chemical and/or physical information from the experimental data. Such data include, for example, the number of intermediates present in a reaction, the rate or equilibrium constants, and the spectra for each one of those intermediates. Multivariate resolution methods have been shown to be useful for the study of biophysical and biochemical processes such as folding/unfolding of proteins or nucleic acids. The present article reviews the most frequently used resolution methods, the limitations on their use, and their latest applications in protein and nucleic acid research.

Biophysical Phenomena↗

PubMed2004

Sagittal reformations of volumetric inspiratory and expiratory high-resolution CT of the lung.

RATIONALE AND OBJECTIVES: The purpose of this study is to determine whether sagittal reformations of volumetric high-resolution computed tomography (CT) provide additional information in evaluating lung abnormalities compared to axial high-resolution CT images. MATERIALS AND METHODS: Twenty-two consecutive patients who underwent combined inspiratory and expiratory volumetric high-resolution CT from May to July 2003 were studied. Using end-inspiratory and end-expiratory sagittal reformatted images, respiratory motion artifacts were evaluated at upper, middle, and lower lung areas bilaterally. Diaphragmatic motion artifacts were evaluated bilaterally. The detection of major and minor fissures was assessed. Additional findings on sagittal reformations were graded using a three-point scale: grade 1 = no additional findings; grade 2 = additional findings of no diagnostic significance; grade 3 = additional findings of diagnostic significance. RESULTS: Respiratory motion artifacts were either imperceptible or present but not diagnostically limiting except for one case, in which diagnostically limiting image degradation was noted in the bilateral lower lung areas. Diaphragmatic motion artifacts were frequently present: 95% (21/22) at end-inspiration, and 86% (19/22) at end-expiration; however, diagnostically limiting artifacts were observed in only two cases. The grading of additional findings on sagittal reformatted images was statistically significant (grade 1, n = 13; grade 2, n = 7; grade 3, n = 2; median, 1; P = .004) compared to axial images. Additional findings included relationship between mass/nodules and fissure/pleura/pericardium (grade 2, n = 4; grade 3, n = 2), distribution of interstitial abnormalities of the lung (grade 2, n = 2), and diaphragmatic eventration (grade 2, n = 1). CONCLUSION: Sagittal reformatted images of volumetric high-resolution CT provided additional information compared to volumetric axial images alone with statistical significance.

Adult↗

PubMed2004

Association of the timing of ST-segment resolution with TIMI myocardial perfusion grade in acute myocardial infarction.

BACKGROUND: More complete ST-segment resolution (ST res) in acute myocardial infarction (MI) has been associated with better epicardial and myocardial reperfusion as assessed with the Thrombolysis in Myocardial Infarction (TIMI) flow grade (TFG) and the TIMI myocardial perfusion grade (TMPG), respectively. However, no data exist comparing the speed of ST resolution on continuous electrocardiogram (ECG) monitoring with the TMPG on coronary angiography. We hypothesized that delayed ST res is associated with impaired TMPGs. METHODS: Continuous 12-lead ECG recordings and 60-minute angiographic data were analyzed in 120 patients with acute MI who received tenectaplase monotherapy or combination therapy with low-dose tenectaplase and eptifibatide in the Integrilin and Tenecteplase in Acute Myocardial Infarction (INTEGRITI) trial. RESULTS: More rapid ST res on continuous ECG monitoring was associated with improved TMPGs on coronary angiography performed 60 minutes after study drug administration. For TMPG 3, the median time to ST resolution was 53 minutes. For TMPG 2, 1, and 0, the corresponding times were 64 minutes, 80 minutes, and 106 minutes, respectively (P =.01 for trend). Likewise, more rapid ST res was also associated with faster epicardial flow. For TFG 3, the median time to ST resolution was 46 minutes, compared with 109 minutes for TIMI flow grades 0 to 2 (P =.001). The corresponding times for a corrected TIMI frame count < or =40 versus >40 were 52 minutes and 112 minutes, respectively (P <.001). CONCLUSIONS: Although the static ECG has been associated with epicardial and myocardial blood flow in the past, this study extends these observations to demonstrate that more rapid ST res on continuous ECG monitoring is associated with improved myocardial perfusion after thrombolytic administration.

Aged↗