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PubMed1994

Activation of dormant cancer cells in the prostates and seminal vesicles of Lobund-Wistar rats.

Lobund-Wistar (L-W) rats are unique in their susceptibility to spontaneous and induced metastasizing adenocarcinomas in the prostate-seminal vesicle (P-SV) complex. Tumors were induced in 70-100% of rats by a combination of i.v. inoculated methylnitrosourea (MNU) followed by a series of subcutaneous slow-release implants of testosterone propionate (TP). Adenocarcinoma cells initiated by MNU in 3-month-old L-W rats were activated significantly by implants of the promoter, TP, after intervening periods of 3, 6 and 12 months following their exposures to MNU. The longer the time between MNU and TP, the shorter the subsequent latent period. Control rats inoculated with MNU (without TP) did not develop tumors during the observation period of 12 months, and their dormant tumor cells were activated by a single implant of TP, thereby eliciting P-SV tumors.

Adenocarcinoma↗
PubMed1989

Comparative effects of verapamil and D-600 on the contractile responses produced by neurotransmitters and depolarizing agents in the rat isolated seminal vesicle.

1. The comparative effects of verapamil and D-600, on isometric contractions produced by noradrenaline, acetylcholine, phenylephrine, clonidine, carbachol and high potassium, were studied in rat isolated seminal vesicle. 2. The aim was to see if verapamil and D-600 differed, in their selectivity, of blocking calcium, channels, and hence inhibition of isometric contractions produced in this preparation. 3. Verapamil (1 microM) and D-600 (9.5 microM), selectively, reduced the isometric contractions, greater reductions occurred in the KCl-induced contractions (control EC50 19 +/- 1.5 mM, in verapamil 58 +/- 1.3 mM, and in D-600 37 +/- 2.0 mM, means +/- SE, n = 8, P less than 0.001, less than 0.01, respectively). 4. It was concluded that KCl-induced contractions were voltage-dependent, whereas noradrenaline operated via both voltage and receptor-mediated mechanisms. 5. The clinical relevance of these results was discussed, and it may help our current understanding of the pharmacological management of sexual dysfunction.

Acetylcholine↗
PubMed1979

A study of the enzyme activity in the seminal vesicles of castrated and hormone-replaced castrated mice.

Castration provokes a time-related decrease in weight, protein, beta-glucuronidase and glucose-6-phosphate dehydrogenase activity of seminal vesicles. A dose-dependent stimulation of these parameters is obtained with 5alpha-DHT. Cryproterone acetate counteracts the stimulatory effects due to androgen. Acid and alkaline phosphatases remain largely unaffected by these treatments.

Acid Phosphatase↗
PubMed2004

Quantifying the impact of seminal vesicle invasion identified using endorectal magnetic resonance imaging on PSA outcome after radiation therapy for patients with clinically localized prostate cancer.

PURPOSE: This study examined the impact that seminal vesicle invasion (SVI), observed on endorectal magnetic resonance imaging (erMRI), had on prostate-specific antigen (PSA) outcome after external beam radiation therapy (EBRT) for patients with clinically localized prostate cancer. METHODS AND MATERIALS: The study cohort was comprised of 250 patients who received 3D conformal radiation therapy without hormones for clinically localized prostate cancer between 1992 and 2001. The primary end point was PSA failure, defined using the American Society for Therapeutic Radiology and Oncology consensus definition. Cox regression multivariable analysis was used to determine the ability of the pretreatment risk group and erMRI SVI to predict for time to PSA failure after EBRT. RESULTS: Both risk group (p(Cox) = 0.001) and erMRI SVI (p(Cox) = 0.003) were independent and significant predictors of time to PSA failure. For patients beyond low risk, 4-year estimates of PSA failure-free survival for erMRI SVI-negative vs. erMRI SVI-positive patients were 68% vs. 33% (p(log-rank) = 0.0014), respectively. CONCLUSION: Patients with clinically localized disease and PSA >10 or biopsy Gleason score >or=7 or clinical T category T2b or T2c who also have erMRI evidence of SVI have PSA outcomes similar to patients with locally advanced prostate cancer after EBRT monotherapy. Consideration should be given to combining EBRT with hormonal therapy in these patients.

Adenocarcinoma↗
PubMed1991

Magnetic resonance imaging of a seminal vesicle cyst and a review of the imaging modalities.

Magnetic resonance imaging (MRI) is invaluable in the diagnosis of pelvic pathology as demonstrated here by its use in the diagnosis of a seminal vesicle cyst. Comparison is made with computed tomography, ultrasonography and voiding cystourethrography (VCUG). MRI provides precise, anatomical detail, far superior to other modalities. The advantages and disadvantages of older diagnostic modalities, such as intravenous pyelogram, VCUG and seminal vesiculography, are discussed in this article.

Adolescent↗
PubMed1982

Structure of secretory protein IV from rat seminal vesicles.

The complete amino acid sequence of rat SVS-IV protein, consisting of 90 residues, has been determined. The sequence of rat SVS-IV protein is the first seminal vesicle secretory protein determined and it does not show any homology with other known protein sequences. The secondary structure of SVS-IV protein is analyzed by methods of Fasman and Chou indicates that this protein contains 53% alpha-helix, 36% beta-turn and 11% random coil.

Amino Acid Sequence↗
PubMed1981

p-Chlorophenylalanine-induced proliferation of the seminal vesicle epithelium.

Intraperitoneal injection of p-chlorophenylalanine (pCPA) methylester (100 mg/kg body weight) results in an activation of the lysosomal system of the secretory cells in the rat seminal vesicle and an elevation of the activities of lysosomal enzymes within 15 min following the injection. Large autophagic vacuoles are formed, sequestering rough endoplasmic reticulum and part of the Golgi apparatus within 2 h. Shortly after the activation of the lysosomal system an elevation of both DNA- and protein synthesis is measured biochemically. 6 h subsequent to the injection a wave o mitoses of the secretory cells begins, reaching a maximum of 6 h later and then declining within 3 h. About 12 h following the injection a second rise in lysosomal activity begins, declining within 24 h. The entire sequence of lysosomal and proliferative activities is inhibited in antiandrogen-pretreated rats. Deduced from these findings the following hypothesis of growth regulation of the accessory sex glands is advanced: enhanced loss of intracellular material during autophagocytosis diminishes the intracellular concentration of a substance curtailing cell division below its effective threshold resulting in division of the secretory cells. The prerequisites of this mechanism are (i) a sufficient distributive capacity of the stroma for hormones (androgens) and metabolic precursors, and (ii) sufficient capacity of the basal cells for transporting the precursors to the secretory cells. Sloughing of the secretory cells separates them from these auxiliary structures (stroma and basal cells) and enables the basal cells to divide.

Animals↗
PubMed1987

Quantitative studies of steroid bioconversions in the seminal vesicles of spawning male African catfish, Clarias gariepinus (Burchell), under natural conditions, and of non-spawning catfish under natural and fish farm conditions.

1. Steroid bioconversions in the seminal vesicles of Clarias gariepinus were studied quantitatively in vitro by tissue incubations with [3H]pregnenolone, [3H]androstenedione and [14C]11 beta-hydroxyandrostenedione, respectively. 2. Spawning and non-spawning catfish, collected in the Hula nature reserve in northern Israel during the spawning period, and non-spawning animals, collected from a fish pond in the same region during the same period, were studied. 3.Spawning animals showed a significantly higher production of 17 alpha-hydroxyprogesterone, 5 beta-pregnan-17 alpha-ol-3,20-dione and 5 beta-reduced androgens than non-spawning feral and pond catfish, as a result of a significantly increased contribution of the enzymes 5 beta-reductase and 3 alpha-hydroxysteroid dehydrogenase (HSD). 4. In spawning catfish the concentration of gonadotropin in blood plasma were also significantly higher than in the plasma of non-spawning feral and pond catfish. This increase in gonadotropin level might have induced the rise in enzyme activity of 5 beta-reductase and 3 alpha-HSD. 5. It is concluded that the absence of a shift in steroidogenesis towards the production of 5 beta-reduced steroids may be among the factors preventing spontaneous spawning in male African catfish under husbandry conditions.

Androstenedione↗
PubMed1980

Nuclear acceptor sites for androgen-receptor complexes in seminal-vesicle epithelium.

An assay method in vitro was developed and applied to quantify acceptor binding of steroid-receptor complexes in nuclei from isolated epithelium of guinea-pig seminal vesicle. Steroid-receptor complex prepared from 1-day-castrated animals was incubated with purified nuclei from 1-28 day-castrated animals in a medium containing 0.15 M-KCl. Free and bound steroid-receptor complexes were measured and the data were submitted to Scatchard analysis. With nuclei from 1-day-castrated animals the Kd for binding of cytosolic [3H]dihydrotestosterone-receptor complexes was found to be 0.83 X 10(-10) M and the capacity for binding was 0.35 pmol/mg of nuclear DNA. Scatchard analysis consistently disclosed only a single line of constant slope and gave the same kinetic constants for nuclei obtained from animals castrated up to 28 days before assay. Administration of 2 mg of dihydrotestosterone, 3 alpha-androstanediol or androsterone or 100 microgram of oestradiol-17 beta 1 h before killing of the 1-day-castrated animals that provided the nuclei resulted in a significant decrease in nuclear acceptor binding of the steroid-receptor complex compared with untreated animals. Thus our assay method disclosed nuclear acceptor sites that may be involved in responses to androgens (and oestrogens) in vivo. We conclude that there is a class of nuclear accept or sites of high affinity and limited capacity that may be occupied by steroid-receptor complexes in vivo.

Animals↗
PubMed2003

A nomogram to predict seminal vesicle invasion by the extent and location of cancer in systematic biopsy results.

PURPOSE: We determined whether systematic biopsy results increases the accuracy of standard clinical information in predicting seminal vesicle invasion (SVI). MATERIALS AND METHODS: We analyzed a retrospective cohort of 763 patients with clinical stages T1c-T3 prostate cancer who were diagnosed by systematic biopsy and treated with radical prostatectomy. We recorded the location of each biopsy core and measured the length of cancer and total length of each core. Using logistic regression analysis we constructed and internally validated a nomogram to predict SVI. RESULTS: A total of 60 patients (7.9%) had SVI. Cancer was present in a biopsy core from the base in 437 patients, of whom 12.8% had SVI compared with only 1.2% of the 326 without cancer at the base. None of the 275 patients with prostate specific antigen (PSA) 10 ng/ml or less and no cancer at the base had SVI. On multivariate analysis serum PSA (p <0.0005), primary Gleason grade (p = 0.028) and percent cancer at the base (p <0.005) were the only significant predictors of SVI. The predictive accuracy of a standard model that included only stage, grade and PSA was maximally enhanced by including the percent cancer at the base (p = 0.0013). A nomogram that incorporated this variable produced probabilities of SVI that differed from the standard model by +/- 10% in 68% of the cases. CONCLUSIONS: The presence and amount of cancer in systematic needle biopsy cores from the base of the prostate strongly predicts the presence of SVI. Systematic biopsy results enhance the accuracy of nomograms to predict SVI.

Adult↗
PubMed1975

The synergistic action of alpha-melanocyte-stimulating hormone and testosterone of the sebaceous, prostate, preputial, Harderian and lachrymal glands, seminal vesicles and brown adipose tissue in the hypophysectomized-castrated rat.

Alpha-Melanocyte-stimulating hormone was shown to act synergistically with testosterone to stimulate the sebaceous, prostate and the seminal vesicles in hypophys-ectomized-castrated rats. The sebaceous glands differed from the other three organs in that alpha-MSH not only acted synergistically, but also had a significant effect which was independent of the presence of exogenous testosterone. The response of the brown adipose tissue to testerone, considerably reduced by hypophysectomy, was not restored by alpha-MSH. The Harderian and lachrymal glands were also pituitary-dependent and their weights in hypophysectomized-castrated rats were not restored by alpha-MSH.

Adipose Tissue, Brown↗
PubMed2003

Ultrastructural changes in subepithelial capillaries and their surrounding stroma in rat prostate and seminal vesicle after castration.

The purpose of this study was to clarify the androgen-ablation-induced morphological changes in the capillaries and stroma near the epithelial cells in prostate and seminal vesicles (SV) using transmission electron microscopy (TEM). In ventral prostate (VP) and SV of immature male rats, the luminal areas of subepithelial capillaries and the width of the stromal layers between endothelia and epithelia were measured quantitatively after castration using TEM and a computed image analyzer. The luminal areas of the capillaries were significantly reduced in VP and SV in the short-term after castration. In the stromal layer, the width of the collagen layer surrounding the capillary significantly increased in VP and SV in the long-term after castration. These data suggest that the reduction of the capillaries and the thickening of collagen surrounding them are related to the involution of glandular epithelial cells in VP and SV after castration.

Animals↗
PubMed1983

Immunofluorescent localization of a seminal vesicle proteinase inhibitor in the female reproductive tract of naturally inseminated mice.

The indirect immunofluorescent technique was used to localize a low molecular weight, acid-stable proteinase inhibitor of seminal vesicle origin in the female reproductive tract of mice. In recently inseminated animals (0, 2, and 4 hr postcoitus) the inhibitor was localized in the copulatory plug, on the epithelia of the vaginal fornix and cervix, in the uterine lumen, and on the apical surface of the uterine epithelium. Ten hours postcoitus the inhibitor was found in localized areas on the uterine epithelium, in a sperm-leucocyte mass in the uterine lumen, and in the copulatory plug. The inhibitor was not found in females 24 hr postcoitus. The inhibitor could not be localized in the oviducts of any of the animals tested. The data are interpreted to mean that the inhibitor, transported to the female at ejaculation, coats the surface of the female reproductive tract protecting it from acrosomal enzymes or from invasion by spermatozoa or pathogens.

Animals↗
PubMed1983

Syp-3, a third polymorphic locus for mouse seminal vesicle proteins.

The B10.AKM/Sn congenic strain displays a particular phenotype of mouse seminal vesicle proteins representing the third polymorphic locus of this system. The Svp-3 symbol was assigned to this locus with two codominant alleles, Svp-3a found in the B10.AKM/Sn strain and Svp-3b expressed by all the other strains so far tested. The Svp-3 locus appears tightly linked to Svp-1 on chromosome 2.

Alleles↗
PubMed1997

Hydatid cysts of the kidney, seminal vesicle and gluteus muscle.

Hydatid disease has a high incidence in Turkey and causes significant complications. A case is reported here of unusual localization of hydatidosis, with involvement of hydatid cysts of the seminal vesicle, kidney and gluteus muscle. A review of the literature is also included.

Aged↗
PubMed1992

Comparison of 35S- and digoxigenin-labeled RNA and oligonucleotide probes for in situ hybridization. Expression of mRNA of the seminal vesicle secretion protein II and androgen receptor genes in the rat prostate.

The sensitivity of radiolabeled and digoxigenin-labeled RNA probes and synthetic oligonucleotide probes for the detection of seminal vesicle secretion protein II (SVS II) and androgen receptor (AR) mRNA was compared by in situ hybridization in paraformaldehyde-fixed cryostat sections of the rat prostate. Both genes are expressed in different amounts in the various prostatic lobes and contiguous glands. SVS II or AR RNA probes were either labeled with digoxigenin-11-UTP or [35S]UTP by in vitro transcription. A synthetic SVS II oligonucleotide probe was 3' end-labeled (tailed) with either digoxigenin-11-dUTP or [35S]dATP. Hybridized 35S-labeled probes were detected by autoradiography and digoxigenin-labeled probes by immunohistochemistry using alkaline phosphatase conjugated anti-digoxigenin antibody or gold-labeled antibody followed by protein A-gold and silver enhancement. Digoxigenin-labeled probes provided the same degree of sensitivity as their 35S-labeled counterparts for the detection by in situ hybridization of weakly and strongly expressed mRNA. Using both labeling methods, the SVS II RNA probes were more sensitive than the oligonucleotide probes and background labelling of the 35S-labeled oligonucleotide probe was high. The digoxigenin method produced less background with all probe types, hybridization signals showed higher resolution and results were obtained faster than with radiolabeled probes. The immunogold silver enhancement system provided the fastest detection of digoxigenin-labeled probes with a sensitivity and resolution similar to that provided by alkaline phosphatase anti-digoxigenin immunohistochemistry. It is concluded that digoxigenin probe labeling and detection provides a sensitive, reliable, and efficient alternative to radiolabeled probes for in situ hybridization of mRNA.

Animals↗