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At least 109 records · Page 6Linked to original sources
PubMed1969

Loss of lysis inhibition in filamentous Escherichia coli infected with wild-type bacteriophage T4.

The plaque enlargement of wild-type T4 bacteriophage observed when assayed in the presence of low concentrations of mitomycin C or after exposure to very low doses of ultraviolet light was studied by using solid as well as liquid culture media. It was found that the filamentous cell formed by the treatment with the agents is responsible for the phenomenon. The filamentous cell was also shown to be characterized not only by the loss of capacity of lysis inhibition but also by a shortening of the latent period. No difference in cellular rigidity could be seen between the filamentous cell and normal cell as far as the analysis from the outside of the cell was concerned, whereas the former cell was shown to be more readily susceptible to phage-induced lysozyme from the inside of the cell. A possible change in the membrane of the filamentous cell and a possible mechanism for lysis inhibition are discussed.

Bacteriolysis↗
PubMed1985

Molecular analysis confirms food source and simultaneous involvement of two distinct but related subgroups of Salmonella typhimurium bacteriophage type 10 in major interprovincial Salmonella outbreak.

More than 2,000 confirmed cases of food poisoning occurred in the four Atlantic provinces of Canada and in Ontario during the second and third quarters of 1984. Salmonella typhimurium phage type 10 was identified as the etiologic agent, and cheddar cheese was implicated as the source of infection. Strains isolated from infected humans and from cheese were indistinguishable by biotyping, antibiotic resistance typing, and phage typing. Plasmid analysis confirmed cheese as the source of infection and revealed the presence of two molecular subgroups of bacteriophage type 10. Group I strains carried 57-, 22.3-, and 3.4-kilobase (kb) plasmids; group II strains carried 57-, 4.6-, and 3.4-kb plasmids. Digestion with endonucleases HaeIII, HpaII, and AvaIII indicated that the 3.4-kb plasmids were identical. This outbreak was, therefore, caused by a mixed infection with two distinct but related bacteria. Group I strains are fairly common among Canadian S. typhimurium phage type 10 isolates, whereas group II strains appeared to be unique to this outbreak.

Canada↗
PubMed1997

Cloning and characterization of a cDNA encoding a bacteriophage-type RNA polymerase from the higher plant Chenopodium album.

We have cloned a full-length cDNA from the higher plant Chenopodium album coding for a single subunit bacteriophage-type RNA polymerase. The cDNA isolated from an actively growing cell suspension culture recognized a 3.8 kb transcript on Northern blots. The open reading frame comprises 987 amino acids with a predicted molecular mass of 112 kDa. A comparison of the protein sequence with those of the two known fungal mitochondrial RNA polymerases, from Saccharomyces cerevisiae and Neurospora crassa , reveals extensive homology between the three enzymes. with complete conservation of all catalytically essential amino acids. The putative mitochondrial RNA polymerase from C.album , as well as homologous sequences from rice and barley, which have been partially cloned, lack two catalytically non-essential regions of up to 176 amino acids near the C-terminus present in the two fungal mitochondrial RNA polymerases. The extreme N-terminus of the cloned C.album RNA polymerase displays features of a potential mitochondrial transit sequence. In phylogenetic trees constructed to compare the evolutionary relationships between the different single subunit RNA polymerases the C.album sequence forms a subgroup together with the S.cerevisiae and the N.crassa mitochondrial RNA polymerases, well separating from both bacteriophage enzymes and plasmid-encoded RNA polymerases found in mitochondria of many fungi and some higher plants.

Amino Acid Sequence↗
PubMed1990

[Correlations between lactose-fermenting capacity, sensitivity to antibiotics and bacteriophage types of Salmonella agona strains].

Correlation between the ability to ferment lactose, sensitivity to chemotherapeutic agents and phage type of lactose fermenting and nonfermenting S. agona strains was shown in this study. The difference in sensitivity of two S. agona variants toward gentamicin, tobramycin and chloramphenicol was shown. Lactose fermenting S. agona strains were resistant to the above listed antibiotics. The percentage of the resistant strains was 95.3%, 96.9% and 95.3%, respectively. S. agona strains typable by biochemical tests were more often sensitive to those antibiotics. The percentage of the resistant strains of S. agona with typical biochemical properties was for gentamicin 19.4%, tobramycin 3.7% and chloramphenicol 6.7%. Moreover, the percentage of lactose fermenting S. agona strains resistant to seven other out of seventeen tested antibiotics was 83.8% on average and it was 20% higher than percentage of biochemically typical S. agona strains resistant to the same antibiotics. Lactose fermenting S. agona strains belonged almost four times more frequently to subtype VB and also more often to phage type XI; on the other hand six times rare to phage type I in comparison to biochemically typical S. agona strains.

Anti-Bacterial Agents↗