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Effect of background color and low temperature on skin color and circulating alpha-MSH in two species of leopard frog.

Circulating levels of alpha-melanocyte stimulating hormone (alpha-MSH) in two species of leopard frog, Rana pipiens and R. chiricahuensis, were measured by radioimmunoassay to reveal the correlation between skin color change induced by background color and by low temperature. High levels of alpha-MSH were found in both species of frog on a black background, but R. chiricahuensis had eight times higher levels than R. pipiens, R. chiricahuensis also exhibited the ability to darken its ventral surface, whereas the ventral surface of R. pipiens remained white. Neither skin color nor plasma alpha-MSH of R. pipiens was affected by cold. Low temperature did, however, darken dorsal and ventral skin of R. chiricahuensis in vivo, which corresponded to increased levels of plasma alpha-MSH. Dorsal and ventral skin of R. chiricahuensis, in vitro, darken in a dose-dependent manner to alpha-MSH, but not to cold.

Adaptation, Physiological

Cellular mechanisms for color-coding in holostean retinas and the evolution of color vision.

Electrophysiological recording and microspectrophotometry were used to analyze retinal function in representatives of the two surviving genera of holostean grade fish--the bowfin (Amia calva) and gars (Lepisosteus sp.). The properties of the cone photopigments, horizontal cells and ganglion cells show that these holostean retinas have cellular mechanisms for color vision which are fundamentally similar to those previously described for teleosts, turtle and mammals. These findings suggest that trichromatic receptor systems and opponent color-coding mechanisms may have evolved in primitive Neopterygii or more ancient fish, before the advent of teleosts. In conjunction with other recent data on living representatives of primitive fishes, these findings also add renewed plausibility for the view that vertebrate color vision could have taken a common origin some 400 million years ago from an ancestral aquatic jawed vertebrate.

Action Potentials

Early color deprivation and subsequent color vision in a dichromatic monkey.

A squirrel monkey (Saimiri sciureus) was reared for the first 4 months of life in a dim, colorless environment. Following an additional 10 months of normal visual experience, tests of color vision and spectral sensitivity were run on this animal and a control subject. The results suggest that the adult expression of dichromatic color vision does not depend on color experience during the first 4 months of life.

Animals

Perivascular color artifact: pathologic significance and appearance on color Doppler US images.

A distinctive type of artifactual extravascular assignment of color was identified in 49 color Doppler ultrasound (US) examinations in 43 patients. This artifact appeared as a random localized mixture of red and blue assigned to perivascular soft tissues; the artifact varied with the cardiac cycle, being most prominent in systole and absent or less prominent in diastole. The artifact was seen in 26 patients (32 examinations) at an anastomotic site or stenotic lesion associated with surgically created arteriovenous fistulas for hemodialysis. It was also observed in ten patients with accidental iatrogenic arteriovenous fistulas (renal transplant [n = 6], femoral artery [n = 3], and iliac artery [n = 1]), five with stenotic arteries not associated with arteriovenous fistulas, and two with arterial aneurysms. The authors believe this artifact reflects perivascular tissue vibration caused by turbulent intravascular blood flow. If properly recognized and accurately interpreted, the artifact can be a valuable color Doppler US sign of underlying vascular abnormality.

Aneurysm

Large color differences and the geometry of Munsell color space.

Multidimensional scaling of subjective similarity estimates for colors varying extensively in Munsell hue, value, and chroma produced a configuration that concurred with the postulated organization of Munsell color space, and indicated step-size relationship between attributes. Implications for the determination of color difference are discussed.

Color Perception Tests

Asymmetric color matching: how color appearance depends on the illuminant.

We report the results of matching experiments designed to study the color appearance of objects rendered under different simulated illuminants on a CRT monitor. Subjects set asymmetric color matches between a standard object and a test object that were rendered under illuminants with different spectral power distributions. For any illuminant change, we found that the mapping between the cone coordinates of matching standard and test objects was well approximated by a diagonal linear transformation. In this sense, our results are consistent with von Kries's hypothesis [Handb. Physiol. Menschen 3, 109 (1905) [in Sources of Color Vision, D. L. MacAdam, ed. (MIT Press, Cambridge, Mass., 1970)]] that adaptation simply changes the relative sensitivity of the different cone classes. In addition, we examined the dependence of the diagonal transformation on the illuminant change. For the range of illuminants tested, we found that the change in the diagonal elements of the linear transformation was a linear function of the illuminant change.

Color Perception

The Luscher Color Test: sex differences in color preference.

Sex differences in color preference on the Luscher Color Test were investigated for the green and the yellow chips. 50 male and 79 female majors in education rated and ranked each color on the popular form of the test. Females ranked and rated yellow significantly higher than did the males. Implications regarding test validity for females are discussed.

Choice Behavior

Preference for color and form in preschoolers as related to color and form differentiation.

The preference for color or form as bases for similarity judgements among preschoolers (ages 2-5) and its relationship to the differentiation of form and color concepts as indexed by discrimination, identification, and labeling were investigated. As hypothesized, an early stage of form preference was found, replicating Brian and Goodenough's results. This was followed by the often-reported color preference and subsequent shift to form preference. A second hypothesis-that the relative salience of a dimension develops in parallel to its relative degree of differentiation-was not supported. This was so despite the fact that, within age groups, individuals who displayed higher proficiency in one dimension that in the other also preferred that dimension over the other. Implications for for existing explanations of developmental trends in preference were discussed.

Age Factors

[Studies on coloring constituents in commercial madder color].

Two main coloring constituents in the commercial madder color were isolated and identified as ruberthric acid and lucidin-3-O-primeveroside. On quantitative analysis by high-performance liquid chromatography, the contents of ruberthric acid and lucidin-3-O-primeveroside in commercial madder color were determined 0.07% and 0.04%, respectively.

Anthraquinones

Color and form perception on the Rorschach as a function of eye color.

Recent research had demonstrated that dark-eyed individuals are more reactive than light-eyed people. The present paper investigated one area of reactivity, ease of emotional arousal. The hypothesis that dark-eyed male subjects (n equals 40) would show greater ease of arousal than light-eyed male subjects (n equals 40) was tested using color and form responses on the Rorschach as dependent measures. The predicted interaction was significant; light-eyed subjects gave relatively more form responses, but dark-eyed subjects gave relatively more color responses. Implications for future research and application were discussed.

Arousal

Homogeneity of large-field color matches in congenital red-green color deficients.

Rayleigh matches obtained from red-green color deficients with conventional methods show large individual differences within diagnostic categories. Similar matches obtained from the same observers with a large-field substitution method show much less variability and suggest that the differences observed among simple anomals, extreme anomals, and dichromats with conventional methods are probably not solely due to the visual pigments contained in the cones. A theory that attributes these differences to the relative number of abnormal cones present in the observer's retina is described.

Color Perception

Color-contrast staining of two different lymphocyte subpopulations: a two-color modification of alkaline phosphatase monoclonal anti-alkaline phosphatase complex technique.

A dual staining method for different human lymphocyte subpopulations with nonoverlapping antigen distribution patterns is described. Cytocentrifuge slide preparations of peripheral blood nonadherant mononuclear cells (NAMNC), bone marrow aspirate or buffy coat smears were fixed in acetone and incubated with a primary mouse monoclonal antibody (MAb) against a lymphocyte antigen (CD8, Ig-light-chain, CD19, CD4) followed by rabbit anti-mouse immunoglobulin (Ig) and the alkaline phosphatase monoclonal anti-alkaline phosphatase (APAAP) complex. After repeating the "bridge" antibody and the APAAP, a red product was developed with fast red TR-naphthol AS-BI phosphate. Following this one-color stain the process was repeated using a different primary mouse MAb against another lymphocyte antigen (CD4, Ig-light chain, CD3, MHCII DR, CD5) and fast blue BB-naphthol AS-MX phosphate at the last step to yield a blue product. Control slides stained by the standard one-color APAAP method with the relevant primary MAb showed that there was no nonspecific labelling and the percent of positive cells in a given test was almost identical. To achieve an intense blue in the second stain for some antigens, e.g., CD4, either the MAb concentration had to be increased or two different MAbs recognizing differing epitopes of the same antigen, e.g., T1 and UCHT2 for CD5, were applied. Any change of red to purple at the site of the first stain after 15 min exposure to the blue-yielding AP substrate is due to residual AP activity of the first stain rather than to crossbinding of immunoreagents.(ABSTRACT TRUNCATED AT 250 WORDS)

Alkaline Phosphatase

Effects of the color additive caramel color III and 2-acetyl-4(5)-tetrahydroxybutylimidazole (THI) on the immune system of rats.

Administration of ammonia caramel color (AC) to rats may decrease blood lymphocyte counts, specifically in rats fed a diet low in vitamin B6. This effect is associated with 2-acetyl-4(5)-(1,2,3,4-tetrahydroxybutyl)imidazole (THI). To characterize and compare the effects of AC and THI and to study the influence of dietary pyridoxine, two studies in rats were conducted. Weanling rats fed a diet containing 2-3 ppm pyridoxine and exposed to 4% AC or 5.72 ppm THI in drinking water for 4 weeks showed reduced cell numbers in spleen and popliteal lymph nodes, as well as in the blood. Flow cytometric analyses demonstrated a comparable reduction in B and T lymphocytes. In blood, spleen, and popliteal lymph nodes, CD4+ lymphocytes were more reduced than CD8+ cells. The number of bone marrow cells was not affected. Although thymus weight and cell number were not affected either, a decreased cortex over medulla area ratio and an increase in medullary cell density largely due to an increase in CD4+ thymocytes was observed. Decreased numbers of ED2+ macrophages were observed in the thymic cortex and in the spleen red pulp. All effects observed were either less pronounced or absent in a study with rats fed a diet containing 11-12 ppm pyridoxine. The effects of AC and THI on the immune system were similar. Whereas AC exposure was associated with changes in vitamin B6 status, THI did not induce obvious effects on vitamin B6 parameters. It is proposed that the effects of AC and THI on the immune system are not caused by a disturbance of vitamin B6 metabolism, but may in fact result from a disturbance of a specific PLP-dependent process.

Administration, Oral

Report on a group form for the measurement of color-word interference: speed of color discrimination test.

Although there have been some suggestions towards the development of a standardized form for a group version of Stroop's color-word interference test yet, to some extent, a group form has not been applied in psychological research. In this study the equivalence of group and individual procedures as well as some standards for psychological tests of the group form were examined. The results show that the interference score does not comply with the requirements of psychological tests and the equivalence of the interference scores of the group and individual procedures could not be determined. For further research experimental analysis of convergent and discriminant validity is suggested.

Adult