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At least 109 records · Page 6Linked to original sources
PubMed2005

Effect of self-etching adhesives on dentin permeability in a fluid flow model.

STATEMENT OF PROBLEM: Numerous self-etching bonding systems exist, with composition differing from one product to another. It is important for the clinician to know if they are all equally effective, and whether they provide an effective seal between dentin and restorative materials. PURPOSE: This study was designed to measure the hydraulic conductance of physiologic saline across dentin after application of various self-etching bonding systems or of a 1-bottle adhesive system preceded by a phosphoric acid etch. MATERIAL AND METHODS: One hundred extracted noncarious human third molars from patients 18-25 years old were used for this study. Dentin disks were cut from crown segments parallel to the occlusal surface at the top of the pulp cavity. The 100 disks, each 1 mm thick, were divided into 10 groups (n=10 per group), each of which was treated with 1 of 9 self-etching systems-AdheSE, Adper Prompt L-Pop, Clearfil SE Bond, Etch & Prime 3.0, Prime & Bond NRC Nt, One-Up Bond F, optiBond solo Plus Self Etch, Prompt L-Pop, or Xeno III-or a control bonding system (Prime & Bond NT) preceded by a phosphoric acid etch. Hydraulic conductance, the volume of fluid transported across a known area of surface (0.28 cm2) per unit time under a unit pressure gradient (200 cm H2O), was analyzed for the adhesive systems using a fluid flow apparatus (Flodec). First, both sides of each specimen were etched with 36% phosphoric acid for 30 seconds, and the hydraulic conductance was measured every 30 seconds for 15 minutes. The initial set of measurements served as the reference value for each specimen. The measurements were repeated when a smear layer had been formed and, finally, after 1 of the 10 bonding systems had been applied. The data were analyzed using a 1-way ANOVA and Duncan's multiple range test (alpha=.05). RESULTS: The control 1-bottle adhesive used with a phosphoric acid pre-etch did not provide the largest reduction in penetration (42.3%). The greatest mean reduction (68.9%, P<.05) was observed with the self-etching product Xeno III. The mean reduction for the other materials tested were: One-Up Bond F, 60.5%; AdheSE, 58.2%; Etch & Prime 3.0, 56.8%; Adper Prompt L-Pop, 51%; optiBond solo Plus Self Etch, 45%; Clearfil SE Bond, 35.5%, Prime & Bond NRC Nt, 16.4%; and Prompt L-Pop, 16.3%. CONCLUSION: Within the limitations of this study, the self-etching systems tested produced different reductions in dentin permeability. For some, the reduction was much greater than the decrease in dentin permeability provided by the bonding system with a phosphoric acid pre-etch; for others, the reduction was small.

Adolescent↗
PubMed2001

2000 Judson C. Hickey Scientific Writing Award. Effect of impression materials on hybridized dentin.

STATEMENT OF PROBLEM: Tooth sensitivity after crown preparation is not an uncommon complication. For dentin bonding agents to be effective in preventing postoperative sensitivity, they must remain intact throughout the fixed prosthodontic procedures. PURPOSE: This study evaluated, by analyzing the change in dentin permeability, whether a dentin bonding agent was removed from the surface of prepared teeth in the process of making an impression. MATERIAL AND METHODS: Eighty extracted human molars were cut horizontally apical to the cemento-enamel junction and horizontally through the middle of the clinical crown. The specimens were prepared to measure dentin permeability (fluid flow rate, Lp). The specimens (n = 40) were randomly assigned to either the primer-only group (PO) or the primer + adhesive group (PA). Five different impression materials were tested: vinyl polysiloxane, polyether, polysulfide, irreversible hydrocolloid, and reversible hydrocolloid. Dentinal permeability was measured after smear layer removal (control, etched Lp = ELp), after coating the dentin with either PO or PA (coated Lp = CLp), and again after making an impression (impression Lp = ILp). A 1-way ANOVA of differences, followed by a Student-Newman-Keuls test (alpha=.05), was performed to compare the ELp, CLp, and ILp values. RESULTS: A statistically significant difference was found between the CLp and ELp for all 5 impression materials in both groups (PO and PA). No difference was found between the CLp and ILp values for any of the impression materials. No significant differences were found between any of the 5 impression materials or either of the dentin surface treatments (PO or PA) when measuring the CLp and ILp. CONCLUSION: The dentin bonding agent had a significant effect in decreasing the Lp of the treated specimens. None of the impression materials significantly affected the measured CLp. There was no significant difference between the PO or PA (CLp) values.

Acid Etching, Dental↗
PubMed2002

Effect of dietary carbamyl phosphate on dentine apposition in rat molars.

In rats, sucrose increases dental caries and impairs odontoblastic function by reducing dentine apposition during primary dentinogenesis. A high-sucrose diet also affects negatively a pulp or dentine function that appears to regulate solute or fluid movement within rat dentinal tissue. In earlier work it was found that carbamyl phosphate could significantly reverse sucrose-induced cariogenesis and also stimulate sucrose-depressed movement of dentinal fluid through a mechanism involving parotid function(s). In the current study, the possibility that carbamyl phosphate could overcome the sucrose-induced reduction in dentine apposition was examined. Weanling rats were fed a high-sucrose diet supplemented or not with carbamyl phosphate for 5 weeks. Dentine apposition was measured planimetrically in sagittal sections of the molars. The effect of carbamyl phosphate was similarly tested in parotidectomized animals. Carbamyl phosphate significantly reduced the deleterious effect of sucrose on dentine apposition by 58% in the first molars. However, the reduction in dentine apposition that followed parotidectomy was not altered by carbamyl phosphate supplementation. The possibility that the beneficial effect of carbamyl phosphate on dentinogenesis involves a parotid function is entertained.

Analysis of Variance↗
PubMed2002

Bacterial adhesins--their role in tubule invasion and endodontic disease.

Bacterial invasion of dentinal tubules is critical to the progression of dental caries and the development of pulp and periapical disease, and may also influence the progression of periodontal disease. However, little is known about the host or bacterial mechanisms involved in tubule invasion. Recent work has demonstrated that bacterial interactions with dentine, and salivary and tissue molecules influence invasion. Salivary molecules such as mucin and immunoglobulin G (IgG) co-aggregate with bacterial cells, which inhibits dentine invasion, while deposition of dentinal tubule fluid molecules e.g. albumin, IgG, or fibrinogen within dentinal tubules also inhibits invasion. Dentine invasion by streptococci has been shown to be associated with a bacterial growth response and adhesion to unmineralized collagen, which are mediated by streptococcal cell-surface antigen I/II polypeptides. These adhesins possess diverse binding properties including binding to salivary glycoprotein, other bacteria, and to collagen. Additionally, some antigen I/II polypeptides facilitate species-specific co-invasion between streptococci and obligate anaerobes that lack the ability to invade by themselves. An understanding of the mechanisms involved in bacterial invasion of dentine should allow development of new control strategies.

Adhesins, Bacterial↗
PubMed1998

Treating hypersensitivity with fluoride varnishes.

Dentinal hypersensitivity results when stimulation causes the fluid in open dentinal tubules to undergo pressure changes, which activates mechanoreceptor nerves and results in pain. Treatment with fluoride varnish forms a protective layer of calcium fluoride that prevents this fluid flow, thereby reducing dentinal sensitivity.

Administration, Topical↗
PubMed1999

Treating hypersensitivity with fluoride varnish.

Dentinal hypersensitivity results when stimulation causes the fluid in open dentinal tubules to undergo pressure changes, which activates mechanoreceptor nerves and results in pain. Treatment with fluoride varnish forms a protective layer of calcium fluoride that prevents this fluid flow, thereby reducing dentinal sensitivity.

Calcium Fluoride↗
PubMed1980

Bacterial penetration of human dentin in vitro.

The ability of bacteria to penetrate acid-etched and unetched human dentin was examined in vitro using a split chamber. Results indicate that bacteria can grow through or be filtered by pressure through acid-etched dentin. Unetched dentin, while permitting fluid filtration, restricts bacterial penetration. Penetration of dentin tubules by bacteria reduces the rate of fluid filtration across dentin.

Acid Etching, Dental↗
PubMed1995

An SEM examination of etched dentin and the structure of the hybrid layer.

The clinical requirements of dentin bonds are that they should be non-permeable to oral fluids, seal dentinal tubules, protect the pulp, and be long lasting and durable. Dentin bonding systems that use acidic agents to remove the smear layer are currently being used. Acid conditioning not only removes the smear layer, but also demineralizes the surface of the intertubular dentin and produces intratubular demineralization and funnelling. A dentin bond is produced when hydrophillic resin monomers infiltrate the dentinal tubules and collagen of the demineralized intertubular zone, producing a hybrid layer. The use of a critical point drying technique and SEM allows a clear visualization of the structure of the hybrid layer. This study showed that currently used hydrophillic resin monomers are unable to completely infiltrate the demineralized zone, and it is speculated that this failure could contribute to microleakage and influence the long-term durability of the bond. It is also apparent that these bonds depend on the mechanical investment of collagen by the infiltrating monomer. Since none of the unfilled resins tested seem capable of completely infiltrating the demineralized collagenous zone, the degree of demineralization produced by the commercial acid concentrations in current use is questioned. More dilute acids than those available commercially are shown to reduce both the degree and depth of demineralization, and we suggest that the resultant thinner layer may lend itself to more complete resin infiltration of the collagen.

Acid Etching, Dental↗
PubMed1978

Dentin permeability: determinants of hydraulic conductance.

A technique is described which permits measurements of the ease with which fluid permeates dentin. This value, the hydraulic conductance of dentin, increased as surface area increases and/or as dentin thickness decreases. It increased 32-fold when dentin was acid etched due to removal of surface debris occluding the tubules.

Acid Etching, Dental↗
PubMed2005

Tubular occlusion prevents water-treeing and through-and-through fluid movement in a single-bottle, one-step self-etch adhesive model.

Water entrapment occurs at resin-dentin interfaces of one-step self-etch adhesives. We hypothesized that by preventing water fluxes from dentin, any water entrapment would be attributed to incomplete removal of adhesive solvents. We tested this hypothesis by bonding to transparent carious dentin containing occluded dentinal tubules. An experimental single-bottle, one-step self-etch adhesive was applied to flat surfaces of caries-affected dentin surrounded by sound dentin, with or without pulpal pressure. Resin-dentin interfaces were examined with TEM after silver-impregnation. Although caries-affected dentin was highly porous, adhesive layers were devoid of silver deposits when tubules were occluded. Conversely, variable extents of water-treeing and water-droplets were identified from adhesive layers in bonded sound dentin. Water-treeing and water-droplet formation, being manifestations of evaporative and convective water fluxes, can be eliminated during bonding to occluded transparent carious dentin. However, the highly porous nature of this clinically relevant substrate after bonding may lead to potentially undesirable consequences.

Acid Etching, Dental↗
PubMed2004

In vivo and in vitro permeability of one-step self-etch adhesives.

Adhesive dentistry should effectively restore the peripheral seal of dentin after enamel removal. We hypothesize that non-rinsing, simplified, one-step self-etch adhesives are effective for minimizing dentin permeability after tooth preparation procedures. Crown preparations in vital human teeth were sealed with Adper Prompt, Xeno III, iBond, or One-Up Bond F. Epoxy resin replicas were produced from polyvinyl siloxane impressions for SEM examination. Dentin surfaces from extracted human teeth were bonded with these adhesives and connected to a fluid-transport model for permeability measurements and TEM examination. Dentinal fluid droplets were observed from adhesive surfaces in resin replicas of in vivo specimens. In vitro fluid conductance of dentin bonded with one-step self-etch adhesives was either similar to or greater than that of smear-layer-covered dentin. TEM revealed water trees within the adhesives that facilitate water movement across the polymerized, highly permeable adhesives. Both in vitro and in vivo results did not support the proposed hypothesis.

Acid Etching, Dental↗
PubMedDate not supplied

Dentinal adhesive with composite restorations: a clinical and microstructural evaluation.

It is generally accepted that the application of dentin-enamel adhesive should involve not only the etched enamel but also the dentinal surface. For this purpose, new bonding agents requiring dentin etching and the combined use of bifunctional groups (Primers) as well as hydrophilic resins have been introduced. This study used scanning electron microscopy to analyze the relationship between dentin and fluid resin of three bonding agents. The results demonstrated that the adhesive monomers easily penetrated the etched dentin, sealing the dentinal tubules and creating a resin dentin interdiffusion zone (hybrid layer). As a result, the microleakage between dentin and resin composite restorations, responsible for postoperative tooth sensitivity, pulpal inflammatory reaction, and secondary decay, is greatly reduced.

Acid Etching, Dental↗
PubMed1999

Scanning electron microscopy (SEM) investigation of selected desensitizing agents in the dentine disc model.

Dentine sensitivity is a painful clinical conditions that can affect up to 35% of the population at any one time. Both professionally available (in-office) or commercially available (over-the-counter) products have been used to treat dentine sensitivity. The aim of the present study was to investigate whether selected in-office desensitizing agents occluded dentine tubules in the dentine disc model. Both surface effects and tubule penetration of the five selected test products were examined by scanning electron microscopy. The results of the present study appeared to demonstrate that all of the applied desensitizing agents produced some occlusion of the tubules although the level of coverage and occlusion varied between the products. Of all the agents tested, ferric oxalate, the active ingredient of Sensodyne Sealant, produced crystal-like structures which occluded a higher proportion of the tubules across the dentine disc surface. ALL-BOND 2 and One-Step (both light-cured primer systems) produced similar crystal-like structures and, although coverage was not uniform across the disc surface, there was some reduction in tubule diameter. These three products, however, appeared to be more effective than either Butler Protect (potassium oxalate) or Oxa-gel (potassium oxalate in a gel) where there was a marked decrease in both the level of coverage and tubule occlusion. Both quantitative and functional studies are required in order to determine the effects of these agents on dentine permeability (fluid flow) as well as clinical studies to determine their effectiveness over time in reducing pain arising from dentine sensitivity.

Dentifrices↗