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[The effect of the addition of dried pig excrement to beef cattle diet on meat quality].

The carcasses and meat and fat properties of a group of five bulls fed a basal diet with an addition of 0.80 kg of dried pig excrements for 135 days were compared with a group of five control bulls fed the basal diet. The pre-mortal examination of the clinical state of the animals, the post-mortal veterinary inspection of the meat and internal organs, and the comparison of blood transaminase activity did not reveal any signs of health disorders ascribable to the experimental diet. The organoleptic examination and determination of the basic and some other technological characteristics and other properties of the meat and fat did not show any significant differences in quality to justify a decision by which the meat of the experimental bulls would be treated as less valuable or non-edible.

Animal Feed↗

Meat quality traits and muscle characteristics in high or low fear lines of Japanese quails (Coturnix japonica) subjected to acute stress.

1. The influence of acute stress before slaughter on muscle and meat quality was studied in adult male quail from lines divergently selected for long (LTI) or short (STI) duration of tonic immobility (TI) and from the unselected (C) control line. 2. When subjected to acute stress, birds from the long TI line responded differently to those of the control or the low fear lines. LTI birds exhibited: a higher plasma creatine kinase and a smaller increase in plasma corticosterone levels, a higher pHu and drip loss values for breast meat associated with no differences in L*a*b* colour values; a higher percentage of fast glycolytic myofibres (IIb) and correspondingly (correlate) higher glycolytic (activity) in the pectoralis major (PM) muscle, and consequently lower oxidative enzyme activity in the PM muscle. 3. It is concluded that acute stress affected muscle metabolism differently in the STI, C and LTI lines. Hence, selection for TI, which is correlated with underlying fearfulness, can indirectly lead to differences in meat quality.

Animals↗

Free-range rearing of pigs during the winter: adaptations in muscle fiber characteristics and effects on adipose tissue composition and meat quality traits.

This research aimed to determine whether outdoor free-range rearing during the winter (average ambient temperature of 5 degrees C) vs. indoor housing (22 degrees C) affects meat quality, muscle metabolic traits, and muscle fiber characteristics. Forty Large White gilts and barrows were blocked by weight within each gender (20 per gender) and allotted randomly into two groups of pigs, with one reared indoors (IN) in individual pens (2.6 m2) and the other reared outdoors (OUT) from December to March in a 0.92-ha pasture. Both groups had free access to the same grower-finisher diet from 23 to 105 kg. At slaughter, adipose (backfat [BF] and omental fat [OF]) and muscle tissues (longissimus muscle [LM], rectus femoris [RF], and semitendinosus [ST]) were obtained from the right side of each carcass. Muscle fibers were stained and classified on the basis of stain reaction as slow-oxidative (SO), fast oxidative-glycolytic (FOG), and fast glycolytic (FG); fiber area and distribution were determined. Also assessed were carcass characteristics, initial and ultimate pH, L*a*b* values, drip loss percent, glycolytic potential (GP), and intramuscular lipid content, as well as the fatty acid profile of each muscle and adipose tissue. The OUT pigs had lower (P < 0.05) ADG and leaner (P < 0.05) carcasses than IN pigs. Rearing environment did not (P > 0.63) affect the intramuscular lipid content of the ST, but intramuscular lipid content was lower (P < 0.01) in the LM and tended to be higher (P = 0.06) in the RF of OUT than in those of IN pigs. In the BF outer layer of the OUT pigs, the higher PUFA content was compensated by both a lower (P < 0.01) saturated and monounsaturated fatty acid (MUFA) content, whereas in the OF, LM, and dark portion of the ST, only the percentage of MUFA was decreased (P < 0.01). In all tissues of the OUT pigs, the linolenic acid content was higher (P < 0.01) and the n-6:n-3 ratio was lower (P < 0.01). The GP of all muscles was higher (P < 0.01), and the ultimate pH of the RF and ST was lower (P < 0.01), in OUT compared with IN pigs. Lightness (L*) values were lower (P < 0.01) in the LM. Percentages of drip loss were higher (P < 0.05) in the LM and light portion of the ST of OUT than in those of IN pigs. The LM and RF of OUT pigs had more (P < 0.01) FOG and fewer (P < 0.01) FG fibers than muscles of IN pigs. Results suggest that rearing pigs outdoors increases aerobic capacity of glycolytic muscles but has little concomitant influence on meat quality traits.

Adaptation, Physiological↗

Mitochondrial function in turkey skeletal muscle--impact on meat quality.

1. M. iliotibialis (MIT) and M. pectoralis (MP) of the BUT Big 6 and Kelly BBB turkey were characterised with respect to physical properties, mitochondrial function, metabolic state, morphology and meat quality. 2. Mitochondrial enzyme activity and respiration rates in MP declined with increasing age while glycolytic enzyme activity remained nearly constant. 3. There were no major differences between BUT Big 6 and Kelly BBB with respect to histological, histochemical or biochemical variables. In spite of the greater adult weight of BUT Big 6, body proportion was equal in both strains. 4. In agreement with the histochemical findings MIT showed higher oxidative capacities, while glycolytic enzyme activity was higher in MP. 5. Pyruvate was the best substrate for oxidative phosphorylation in MIT, but not in MP. Pyruvate dehydrogenase (PDH) activity was up to 15 times less in MP and blood lactate was correlated with intramuscular pH. 6. Turkey breast muscle was restricted in its ability to oxidise pyruvate, especially in those animals that tended to develop intramuscular acidosis post mortem. 7. It is concluded that the in vivo metabolic environment results in acidosis and impaired meat quality, at least in turkey M. pectoralis.

Acidosis↗

Effect of stunning amperage on broiler breast muscle rigor development and meat quality.

Two experiments were conducted to determine the effects of constant amperage (as opposed to constant voltage) electrical stunning on broiler blood loss, post-mortem breast muscle (Pectoralis major) rigor development, and breast meat quality. Broilers were individually stunned for 5 s at 0 (unstunned control group), 50, 100, 150, and 200 mA in Experiment 1 and at 0, 50, and 125 mA in Experiment 2. Breast muscle pH and R-value (ratio of adenosine to inosine nucleotides) were determined at 15 min and 24 h post-mortem; breast meat shear value and color were determined at 48 h post-mortem. Stunning amperage had no effect on percentage blood loss in either experiment. The most rapid post-mortem reactions were observed for the unstunned control group as determined by pH and R-value at 15 min post-mortem. Birds stunned with 50 mA were intermediate with regard to rate of rigor development. The slowest post-mortem reactions occurred in broilers stunned from 100 to 200 mA. There were no differences in pH, R-value, or color between stunning treatments after carcasses were aged for 24 h. Stunning amperage did not affect Allo-Kramer shear value for breast muscles deboned at 15 min post-mortem. In Experiment 2, 24 h aged breast meat from broilers stunned with 125 mA required significantly higher shear value (4.5 kg/g) than breast meat from broilers stunned at 0 or 50 mA (3.8 and 3.6 kg/g, respectively). Results indicate that stunning amperages between 0 and 200 mA had effects on the rate of early rigor development but there were no consistent effects on final breast meat quality.

Animals↗

Carcase and meat quality in ducks killed with either gas mixtures or an electric current under commercial processing conditions.

1. The feasibility of killing 7-week old Peking ducks with gas mixtures and their effects on carcase and meat quality were evaluated and compared with killing in electrical waterbath under commercial conditions. 2. The prevalence of carcase appearance defects and broken bones in the carcases and haemorrhaging, pH, colour, cooking loss and texture of breast muscles were determined. 3. Ducks can be killed within 3 min by exposure to either 90% argon in air or a mixture of 30% carbon dioxide and 60% argon in air. 4. Gas or controlled-atmosphere killing of ducks, whilst they are still in their transport containers, would eliminate some of the welfare concerns associated with the conventional electrical waterbath stunning systems, without adversely affecting carcase and meat quality.

Abattoirs↗

Effects of stunning and decapitation on broiler activity during bleeding, blood loss, carcass, and breast meat quality.

Four experiments were conducted to determine the effects of electrical stunning and decapitation on bird activity as well as carcass and meat quality. In Experiment 1, broilers were subjected to one of four stunning and killing methods: no stun and neck cut, stun and neck cut, no stun and decapitation, and stun and decapitation. Birds were scored for severity of physical activity on a scale of 1 to 4 with 1 being no activity and 4 being severe wing flapping and muscular contractions. Carcasses were also scored for red wing tips and broken bones. In Experiments 2 to 4, all birds were stunned prior to neck cut or decapitation. Carcasses were scored as described in Experiment 1 as well as measurements of blood loss, feather removal, and breast meat pH, color, cook loss, and tenderness. Based on carcass activity in Experiment 1, decapitation following stunning was similar to a conventional stun and unilateral neck cut, except there was almost no late activity (after 60 s) observed in the decapitated birds. Decapitation following stunning did not result in any consistent carcass quality defects compared to conventional killing in the four experiments. No differences were found in 24-h lightness values, yellowness, cook yield, tenderness, and ultimate pH between conventionally killed and decapitated birds. Blood loss and breast meat redness were inconsistent. These results indicate that high frequency stunning and decapitation may be an acceptable alternative to conventional slaughter based on carcass and meat quality and by ensuring an irreversible loss of consciousness.

Abattoirs↗

Effects of endosulfan and amitraz on feedlot performance, carcass yield and meat quality characteristics of Nubian goats.

The pesticides endosulfan and amitraz were fed to 2 groups of Nubian goats (3 goats/group) at 5 mg/kg/d for 36 d to assess their effects on feedlot performance, carcass yield and meat quality. Growth rates, weight gains and feed conversion efficiencies were depressed insignificantly. The endosulfan-dosed group had significantly lower dressing-out percentage and both groups had significant depletions of omental and mesenteric fats. Significant reductions were also seen on body and cavity fats, the latter being severely reduced suggesting more mobilization of cavity fat than body fat. The fat-free body was homogenous with variations in the muscle/bone ratio reflected on carcass cuts. Meat quality was unaffected. Fat was the only chemical component of endosulfan-dosed total lean significantly reduced. In all parameters the effects of endosulfan were more pronounced than those of amitraz.

Administration, Oral↗

[Health, growing performance and meat quality of pigs in indoor and outdoor housing--a controlled field trial].

The purpose of the study was to compare indoor and outdoor housing of pigs and their influence on animal health, growing performance and meat quality in a controlled field trial: 29 litters (252 piglets) from indoor and 22 litters (221 piglets) from outdoor were separated at weaning. One half of each litter changed to the opposite housing resulting finally in 4 types of housing: Continuous outdoor or indoor raising and combined outdoor-indoor or indoor-outdoor raising. Pigs in continuous resp. predominant outdoor housing showed lower morbidity and mortality during all raising periods, more active behaviour, higher daily weight gain in weaned pigs and in fattening pigs, but higher feed consumption as compared to indoor housed pigs. However, feed conversion did not differ significantly. Due to climatic influences differences in morbidity were partly more significant in summer litters (intestinal infections) or in winter litters (lung infections). Outdoor pigs raised in summer resulted less often in meat classification E (lean meat percentage > 55%), however, meat quality, estimated by pH-measures did not differ significantly. We conclude from our findings that outdoor housing of pigs from birth to slaughtering may be a serious alternative to predominant indoor keeping with regard to general welfare and growing performance.

Animal Welfare↗

Meat quality variation in the robotic sorting of pork loins.

A robot was used to make fiber-optic reflectance measurements from 400 to 700 nm in 10-nm increments at six sites, 10 cm apart, along the length of 48 pork loins. Meat quality was assessed in the longissimus dorsi near the thoracolumbar junction using 1) a bag-drip method for fluid loss, 2) a subjective evaluation of wetness, 3) a colorimeter measurement of paleness (CIE L), and 4) a subjective evaluation for Japanese pork color scores (JPCS). Sorting of the loins in the commercial plant from which they originated was correlated (P < .01) with fluid loss (r=.57), with wetness scores (r=-.57), with CIE L* (r=.71), and with JPCS (r=-.64). Laboratory measurements of pH at the site of meat quality assessment were correlated (P < .01) with fluid loss (r=-.61), with wetness scores (r=.65), with CIE L* (r=-.74), and with JPCS (r=.77). Average spectra obtained robotically were correlated (P < .01) with fluid loss (r=.56 at 670 nm, and R=.76 adding 560 and 540 nm), with wetness score (r=-.65 at 480 nm, and R=.75 adding 530 and 570 nm), with CIE L* (r=.76 at 480 nm, and R=.82 adding 690 and 520 nm), and with JPCS (r=-.70). In sorting loins that were all categorized as normal at the plant, mean reflectance data collected robotically were correlated with fluid loss, r=.42 (P > .05) at 700 nm and R=.58 (P > .05) adding 430 nm; with wetness score, r=.25 (P > .05); with CIE L*, r=.58 (P < .025) at 700 nm; and with JPCS, r=-.71 (P < .01) at 700 nm. Thus, as well as detecting obvious PSE loins, the robotic probe also had a limited capability to sort loins all categorized as normal at the plant.

Animals↗

[Studies on carcass and meat quality performance of crossbred pigs with graded proportions of Laiwu Black genes].

Sixty pigs, including pure Laiwu Black (LL), pure Large Yorkshire (YY), 1/2 Laiwu (Ymale x Lfemale), 3/4 Laiwu (Lmale x YLfemale) and 1/4 Laiwu (Ymale x YLfemale), were housed in groups with the same diet until 90 kg for slaughter. The objectives were to investigate the effects of graded proportions of Laiwu Black genes on carcass and meat quality performance. Results indicated that different consanguinity had significant effect on carcass weight, carcass length (CL), ham percentage (HP) and backfat thickness (BT) (P<0.05), and had highly significant effect on eye muscle area (EMA) and lean percentage (LP) (P<0.01). Furthermore, CL, HP, EMA and LP tended to increased gradually, but BT tended to decreased gradually as Laiwu Black genes decreased. For meat quality properties, different consanguinity had significant effect on meat color, marbling score, dry matter and crude protein content of muscles (P<0.05), and had highly significant effect on water loss, drip loss and intramuscular fat content of muscles (P<0.01). Compared with the muscle of Large Yorkshire, that of Laiwu Black and its crossbred pigs were bright red in meat color, high in water holding capacity and abundant in intramuscular fat. Results implied that to give attention to both quantity and quality, Laiwu Black surely has usable precious value in superior pig production. Commercial crossbred pigs should have about 1/4 of Laiwu Black genes.

Animals↗

Quantitative trait locus mapping for meat quality traits in an Iberian x Landrace F2 pig population.

An experimental F2 cross between Iberian and Landrace pig strains was performed to map quantitative trait loci (QTL) for diverse productive traits. Here we report results for meat quality traits from 369 F2 animals with records for pH 24 h postmortem (pH 24 h), muscle color Minolta measurements L* (lightness), a* (redness), and b* (yellowness), H* (hue angle), C* (chroma), intramuscular fat (IMF) and haematin pigment content measured in the longissimus thoracis. Pigs were genotyped for 92 markers covering the 18 porcine autosomes (SSC). Results of the genome scan show evidence for QTL for IMF (SSC6; F = 27.16), pH 24 h (SSC3; F = 7.73), haematin pigments (SSC4 and SSC7; F = 8.68 and 9.47 respectively) and Minolta color measurements L* (SSC4 and SSC7; F =16.42 and 7.17 respectively), and a* (SSC4 and SSC8; F = 8.05 and 7.36 respectively). No QTL were observed for the color measurements b*, H*, and C*. Alternative models fitting epistasis between QTL were also tested, but detected epistatic interactions were not significant at a genome-wise level. In this work we identify genomic regions related with meat quality traits. Improvement by traditional selection methods is complicated, and finer mapping would be required for their application in introgression programs.

Animals↗

Effects of feeding high-oil corn to beef steers on carcass characteristics and meat quality.

To assess the effects of feeding high-oil corn on carcass characteristics and meat quality, 60 yearling steers were fed high concentrate diets containing either control corn (82% of diet), high-oil corn (82% of diet), or high-oil corn at a concentration that was isocaloric with the control diet (74% of diet). After being fed for 84 d, steers were slaughtered. At 72 h postmortem, carcass data were collected and rib sections from five steers grading U.S. Choice and five steers grading U.S. Select from each treatment were collected, vacuum packaged, and aged for 14 d. Three steaks (2.54 cm thick) were removed from each rib for Warner-Bratzler shear force measurement, sensory appraisal, and fatty acid composition analyses. Data were analyzed with treatment as the main effect for the carcass data and treatment, quality grade, and two-way interaction in the model for the longissimus data. The two-way interaction was nonsignificant (P > 0.05) for all variables tested. No differences were detected (P > 0.05) in carcass measurements except for marbling scores and quality grades, both of which were greater (P < 0.05) for carcasses from steers fed the high-oil corn. Overall, 78% of steers fed the high-oil corn graded U.S. Choice compared with 47% for the control and 67% for isocaloric group. Shear force and sensory properties of the longissimus were not different (P > 0.05) among treatments. Steaks from U.S. Choice carcasses rated higher (P < 0.05) for tenderness and tended to rate higher (P < 0.10) for juiciness. Feeding the isocaloric and high-oil diets increased (P < 0.05) linoleic acid, arachidonic acid, and the total PUFA content of lipid extracted from the longissimus. Saturated fatty acid percentage was lowest (P < 0.05) for high-oil corn and highest (P < 0.05) for control, with isocaloric being intermediate. Feeding high-oil corn increased (P < 0.05) pentadecyclic acid, margaric acid, and total odd-chain fatty acid content. Feeding high-oil corn in finishing beef cattle diets enhanced intramuscular lipid deposition and increased unsaturation of fatty acids of the longissimus.

Animal Feed↗

Mapping quantitative trait loci for carcass and meat quality traits in a wild boar x Large White intercross.

An intercross between wild boar and a domestic Large White pig population was used to map quantitative trait loci (QTL) for body proportions, weight of internal organs, carcass composition, and meat quality. The results concerning growth traits and fat deposition traits have been reported elsewhere. In the present study, all 200 F2 animals, their parents, and their grandparents were genotyped for 236 markers. The marker genotypes were used to calculate the additive and dominance coefficients at fixed positions in the genome of each F2 animal, and the trait values were regressed onto these coefficients in intervals of 1 cM. In addition, the effect of proportion of wild boar alleles was tested for each chromosome. Significant QTL effects were found for percentage lean meat and percentage lean meat plus bone in various cuts, proportion of bone in relation to lean meat in ham, muscle area, and carcass length. The significant QTL were located on chromosomes 2, 3, 4, and 8. Each QTL explained 9 to 16% of the residual variance of the traits. Gene action for most QTL was largely additive. For meat quality traits, there were no QTL that reached the significance threshold. However, the average proportion of wild boar alleles across the genome had highly significant effects on reflectance and drip loss. The results show that there are several chromosome regions with a considerable effect on carcass traits in pigs.

Animals↗

The effects of halothane sensitivity on carcass composition and meat quality in HAL-1843-normal pigs.

Objectives of this study were to determine the incidence of halothane sensitivity in pigs that are homozygous normal at the ryanodine receptor nucleotide 1843 (HAL-1843-normal) and the relationships between halothane sensitivity and carcass composition or meat quality. In Exp. 1, piglets (Lines A, B, C, and D; n = 168, 170, 168, and 169, respectively) were obtained from mating a HAL-1843-normal sire line to four HAL-1843-normal dam lines. In Exp. 2, piglets from Lines A and B (n = 87 and 90, respectively) were included with piglets (Lines E, F, G, and H; n = 94, 92, 89, and 89, respectively) obtained from mating four HAL-1843-normal sire lines to a single HAL-1843-normal dam line. Pigs were subjected to 3% halothane at approximately 9 wk of age. In Exp. 1, limb rigidity, blotching of the skin, and muscle tremors were visually assessed, and based on these criteria, halothane sensitivity (HS) was observed in 48% of the pigs. To better characterize this response, a scoring system was developed and used in Exp. 2. Using this system, 25, 42, and 33% of the pigs in E and 40, 33, and 27% of the pigs in Line G were categorized as HS-low (HS-L), HS-intermediate (HS-I), and HS-high (HS-H), respectively. In Lines F and H, 13 and 18% of the pigs were HS-I, and 0 and 2% were HS-H, respectively. No consistent effects due to HS were observed in carcass composition or meat quality; however, when a subset of pigs from Exp. 2 were subjected to more extensive handling and transportation before slaughter, ultimate pH was lower and drip loss was higher in LM from HS-H compared with HS-L pigs (P < 0.05; n = 71). These results demonstrate that some pigs are sensitive to halothane anesthesia even in the absence of the known HAL-1843 polymorphism. Additionally, halothane sensitivity may be associated with inferior pork quality under adverse antemortem conditions.

Anesthetics, Inhalation↗

Detection and characterization of quantitative trait loci for meat quality traits in pigs.

In an experimental cross between Meishan and Dutch Large White and Landrace lines, 785 F2 animals with carcass information and their parents were typed for molecular markers covering the entire porcine genome. Linkage was studied between these markers and eight meat quality traits. Quantitative trait locus analyses were performed using interval mapping by regression under two genetic models: 1) the line-cross approach, where the founder lines were assumed to be fixed for different QTL alleles and 2) a half-sib model where a unique allele substitution effect was fitted within each of the 38 half-sib families. The line-cross approach included tests for genomic imprinting and sex-specific QTL effects. In total, three genome-wide significant and 26 suggestive QTL were detected. The significant QTL on chromosomes 3, 4, and 13, affecting meat color, were only detected under the half-sib model. Failure of the line-cross approach to detect the meat color QTL suggests that the founder lines have similar allele frequencies for these QTL. This study provides information on new QTL affecting meat quality traits. It also shows the benefit of analyzing experimental data under different genetic and statistical models.

Alleles↗

Effects of feed deprivation and transport on preslaughter blood metabolites, early postmortem muscle metabolites, and meat quality.

In practice, poultry have their feed with drawn several hours before being collected and put on transport to the slaughter plant. With the exhaustion of their internal energy stores, the chickens may lack energy to cope with the conditions to which they are subjected. Meat quality is affected by the energy stored in the muscle at time of slaughter and its rate of decrease postmortem. Of 320 broiler chickens, half were subjected to 5 h feed deprivation whereas the others had access to feed until transport. In both groups, half the chickens were transported for 1.5 h, and the rest were transported within 5 min to the on-site slaughter facility. Twenty chickens were equipped with an ultrafiltration-collection device for monitoring glucose and lactate profiles. After slaughter, liver pH was measured. Stimulation of muscle metabolism was minimized by avoiding plucking. Breast muscle samples were taken at 0, 1, 2, 4, and 6 h and analyzed for pH and metabolite concentrations, and meat quality was measured at 96 h. Although liver glycogen was depleted in feed-deprived chickens, feed deprivation and transport for short periods were not found to affect blood glucose or lactate levels nor glycogen levels in the muscle at slaughter. Muscle carbohydrate metabolism was found to come to a complete halt after 6 h, which was not caused by exhaustion of the glycogen store but by the muscle's ability to generate adenosine triphosphate (ATP) after 4 h. At this time, rigor mortis had set in and deboning could be done without risk of cold shortening.

Abattoirs↗

Quantitative trait loci mapping for meat quality and muscle fiber traits in a Japanese wild boar x Large White intercross.

Three generations of a swine family produced by crossing a Japanese wild boar and three Large White female pigs were used to map QTL for various production traits. Here we report the results of QTL analyses for skeletal muscle fiber composition and meat quality traits based on phenotypic data of 353 F(2) animals and genotypic data of 225 markers covering almost the entire pig genome for all of the F(2) animals as well as their F(1) parents and F(0) grandparents. The results of a genome scan using least squares regression interval mapping provided evidence that QTL (<1% genome-wise error rate) affected the proportion of the number of type IIA muscle fibers on SSC2, the number of type IIB on SSC14, the relative area (RA) of type I on SSCX, the RA of type IIA on SSC6, the RA of type IIB on SSC6 and SSC14, the Minolta a* values of loin on SSC4 and SSC6, the Minolta b* value of loin on SSC15, and the hematin content of the LM on SSC6. Quantitative trait loci (<5% genome-wise error rate) were found for the number of type I on SSC1, SSC14, and SSCX, for the number of type IIA on SSC14, for the number of type IIB on SSC2, for the RA of type IIA on SSC2, for the Minolta b* value of loin on SSC3, for the pH of loin on SSC15, and for the i.m. fat content on SSC15. Twenty-four QTL were detected for 11 traits at the 5% genome-wise level. Some traits were associated with each other, so the 24 QTL were located on 11 genomic regions. In five QTL located on SSC2, SSC6, and SSC14, each wild boar allele had the effect of increasing types I and IIA muscle fibers and decreasing type IIB muscle fibers. These effects are expected to improve meat quality.

Animals↗