PubMed Health⌕ Search

SEARCH · PubMed Health

Results for “ROSE BENGAL”

Explore indexed PubMed citations for clinical trials, systematic reviews and public health research. Read source abstracts and follow each citation to its original PubMed record.

Quote a phrase for an exact phrase match. Source license links do not imply unrestricted reuse.

At least 109 records · Page 6Linked to original sources
PubMed1978

Rose Bengal plate agglutination and counterimmunoelectrophoresis tests on spinal fluid in the diagnosis of Brucella meningitis.

Rose Bengal and counterimmunoelectrophoresis, two tests that detect antibodies against different structural antigens, when carried out on spinal fluid permitted rapid diagnosis of human Brucella meningitis. The Rose Bengal test was positive in five out of five patients studied, and counterimmunoelectrophoresis was positive in all but one. The Brucella meningitis was characterized by an increase of immunoglobulin G in the cerebrospinal fluid.

Agglutination Tests↗
PubMed1993

Effect of glutathione on rose bengal photosensitized yeast damage.

Photodynamic treatment of Candida albicans cells in the presence of the sensitizer rose bengal at low concentration (2.5 microM) did not influence cell survival (100% survival after 60 min illumination). Cell damage was observed when 0.16 mM glutathione was added together with the dye to the cell suspension. A 99% cell inactivation was achieved after 15 min illumination. No preincubation of the cells with the sensitizer was needed. The mechanism of the photodynamic damage exerted by the combined action of rose bengal and glutathione was investigated using specific quenchers. The results suggest an enhancement involving stimulation of the singlet oxygen generation. The short term yeast inactivation achieved by a low concentration of the dye and glutathione may find an application in the design of an efficient anticandidose treatment.

Candida albicans↗
PubMed2005

Synthesis, characterization, and cellular uptake of DNA-binding rose bengal peptidoconjugates.

Peptide conjugates of the xanthene dye rose bengal (RB) are described featuring sequences that promote DNA binding. The complexation of these conjugates with DNA causes efficient quenching of the fluorophore singlet state and suppresses singlet oxygen production. When incubated with human cells, the RB conjugates pass through the cell membrane but are not visualized in the nucleus. This behavior is in stark contrast to that exhibited by structurally analogous conjugates containing the unhalogenated xanthene dye fluorescein. These results highlight the marked sensitivity of cell permeability characteristics to subtle structural differences.

Amino Acid Sequence↗
PubMed2000

Role of reactive oxygen species in cardiac preconditioning: study with photoactivated Rose Bengal in isolated rat hearts.

UNLABELLED: Oxygen radical scavengers have been shown to prevent the development of ischemic preconditioning, suggesting that reactive oxygen species (ROS) might be involved in this phenomenon. In the present study, we have investigated whether direct exposure to ROS produced by photoactivated Rose Bengal (RB) could mimic the protective effects of ischemic preconditioning. METHODS: In vitro generation of ROS from photoactivated RB in a physiological buffer was first characterised by ESR spectroscopy in the presence of 2,2,6,6-tetramethyl-1-piperidone (oxoTEMP) or 5,5-dimethyl-1-pyrroline-N-oxide (DMPO). In a second part of the study, isolated rat hearts were exposed for 2.5 min to photoactivated RB. After 5 min washout, hearts underwent 30 min no-flow normothermic ischemia followed by 30 min of reperfusion. RESULTS AND CONCLUSIONS: The production of singlet oxygen (1O2) by photoactivated RB in the perfusion medium was evidenced by the ESR detection of the nitroxyl radical oxoTEMPO. Histidine completely inhibited oxoTEMPO formation. In addition, the use of DMPO has indicated that (i) superoxide anions (O2*-) are produced directly and (ii) hydroxyl radicals (HO*) are formed indirectly from the successive O2*- dismutation and the Fenton reaction. In the perfusion experiments, myocardial post-ischemic recovery was dramatically impaired in hearts previously exposed to the ROS produced by RB photoactivation (1O2, O2*-, H2O2 and HO*) as well as when 1O2 was removed by histidine (50 mM) addition. However, functional recovery was significantly improved when hearts were exposed to photoactivated RB in presence of superoxide dismutase (10(5) IU/L) and catalase (10(6) IU/L). Further studies are now required to determine whether the cardioprotective effects of Rose Bengal in presence of O2*- and H2O2 scavengers are due to singlet oxygen or to other species produced by Rose Bengal degradation.

Animals↗
PubMed2002

Photosensitizer accumulation in spontaneous multidrug resistant cells: a comparative study with Rhodamine 123, Rose Bengal acetate and Photofrine.

The influence both of overexpression of multidrug transporter proteins and of phenotype changes occurring in cells developing spontaneous resistance on the accumulation of photosensitizer molecules was studied on two tumor-derived cell lines (B16, A2780) expressing the MDR-1 phenotype. Rhodamine 123, Rose Bengal acetate (a fluorogenic substrate that is restored to the native active molecule by specific enzyme activity inside cells) and Photofrin were considered. The two resistant variants accumulate Rhodamine 123 to a lesser extent than the respective wild types. Treatment with verapamil markedly enhances Rhodamine 123 accumulation in resistant cells, blocking the drug's extrusion. The amount of Rose Bengal is larger in resistant cells than in wild type cells. Verapamil does not affect drug accumulation, although it significantly impairs the efflux process. The results are explained by the enhancement of both membrane traffic and esterase activity resulting in intracellular Rose Bengal production that counterbalances the increased ability in the outward transport of resistant cells. Photofrin is accumulated to a lower degree in resistant than in wild type cells. Verapamil does not alter the drug accumulation, although the release process is somewhat affected. Different intracellular turnovers of Photofrin take place in the cell variants, and the release of the monomeric fluorescent fractions is greater in resistant than in wild type cells.

Animals↗
PubMed1997

Rose bengal staining and cytologic characteristics associated with lipid tear deficiency.

PURPOSE: To characterize ocular surface features of patients with an unstable tear film caused primarily by a lipid tear abnormality resulting from noninflamed meibomian gland dysfunction. METHODS: Retrospective clinical data and results from rose bengal staining, modified meibography, and impression cytology were reviewed in 78 patients (142 eyes), all of whom had normal tear secretion and clearance verified by the fluorescein clearance test but an unstable tear film evidenced by tear breakup time +/- SD of 3.4 +/- 2.1 seconds (normal, > 8 seconds). RESULTS: Of 201 symptoms, 147 (73%) were presumably caused by an unstable tear film, 46 (23%) resulted from inflammation, and none were diurnally worsened. All patients had meibomian gland dysfunction characterized by poor or no meibum expression, orifice squamous metaplasia, or acinar atrophy. Rose bengal staining was negative in 95 eyes (67%), positive on nonexposure zones in 30 eyes (21%), and positive on exposure zones in 17 eyes (12%). Among 90 eyes receiving impression cytology, six (7%) were normal, 49 (54%) had pure "lytic" changes characterized by disrupted cell-cell junctions of normal small cells in the nonexposure zone, three (3%) had pure squamous metaplasia without mucous aggregates, two (2%) had squamous metaplasia with mucous aggregates (the latter being a frequent finding of aqueous tear deficiency), and 31 (34%) were mixed with lytic changes and squamous metaplasia. CONCLUSION: Preferential distribution of rose bengal staining in the nonexposure zone and lytic cytologic changes without squamous metaplasia characterize lipid tear deficiency and help to differentiate it from aqueous tear deficiency in patients with an unstable tear film.

Adult↗