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Global vs. restricted negative measure of life change events: methodological inquiry.

The present study replicated a previous investigation that compared a global measure of life change events (the Schedule of Recent Experience) with a restricted negative measure of such events (the Life Experience Survey) in association with self-reported anxiety and depressive symptomatology (N = 84). With appropriate sampling methods and data analysis by gender, the previous finding of the superiority of the negative events measure was not supported. The pattern of inconsistent findings for both measures suggests the need for utilizing semi-structured interviews.

Adult

The natural course of schizophrenia: a review of first-admission studies.

In this article, research on the natural course of illness among first-admission schizophrenic patients is reviewed from an epidemiological perspective. Three types of studies are considered: statistical reports dating primarily from the preneuroleptic era; long-term followback studies; and more recent prospectively designed cohort studies. Although relatively more first-admission patients have a positive course than do multiple admissions patients, the findings confirm the substantial heterogeneity in course and outcome. Methodological improvements in first-admission research are suggested, including separating the analyses of first-episode patients from those with past psychotic experiences; employing longitudinal methods for deriving diagnosis; including patients with drug and alcohol problems where appropriate; enlarging the samples either by multisite or multicenter collaborations or by pooling data across studies; and obtaining better data on treatment experiences in naturalistic research.

Female

Computer-aided self-observation psychological stressors in an ICU.

We have developed a procedure for a computer-aided self-observation method in stressful situations. Staff members of an ICU recorded their experiences on a computer placed in the ICU, immediately after having experienced a stressful event. The computer asked for and stored psychologically relevant information about cognitive, affective and behavioural aspects. An external stress memory of an ICU was established, allowing a personal/institution-orientated stress diagnosis to be made on the basis of 192 episodes. Sixteen nurses in an intensive care unit in a Swiss hospital used this methodology for 12 weeks. One hundred and ninety-two stressful events were recorded (12 episodes per person), forming a good sample of the stressful episodes for the unit. To the same 16 nurses and 31 nurses at the same hospital (N total = 47) the Nursing Stress Scale was administered. The results from both groups showed that problems dealing with death and work load were predominant. Nurse characteristics such as levels of training (unskilled, skilled) and experience (number of years working as a nurse) were analysed by different statistical procedures and related to their stress experience.

Adaptation, Psychological

Isolation of ether-resistant enteroviruses from sewage: methodology.

Experiments were conducted to determine whether polio type 1 (Mahoney and coxsackie A8 viruses adsorb onto cotton fibers of sewer swabs. Negative results were obtained. It has been shown that viruses may exist in sewage as free virus particles or as bound (adsorbed) virus particles. The sewer-swab method of sampling is superior because it filters out the bound virus over several days; when collected, it represents a catch (grab) sample at that particular time which may or may not contain free virus. A simple method for the preparation of sewage inocula for virus isolations is described which samples the bound virus fraction. Only ether-resistant viruses can be isolated, and an ultracentrifuge is not required. By this method, an isolation rate between 60 and 80% of positive sewer swabs can be achieved. Corresponding figures of 84 and 96% were achieved by concentration of sewer-swab eluates with an ultracentrifuge. Quantitative studies showed that the virus concentration in raw sewage can be as high as one infectious particle per 0.5 ml.

Adsorption

A Heideggerian hermeneutical analysis of older women's stories of being strong.

As people in our society live longer, affirming the quality of their lived experience becomes more important. The purpose of this study was to ascertain and analyze the meanings of "being strong" as revealed from within the stories of older women. A Heideggerian hermeneutical approach, from a critical feminist perspective, was the methodology used. The sample consisted of 12 women, 7 White and 5 Black, over the age of 65. A team of researchers analyzed the transcripts. Findings consisted of three constitutive patterns: "Surviving," "Finding Strength," and "Gathering the Memories ... Seeing the Patterns."

Adaptation, Psychological

Importance of locally derived birth weight nomograms.

Significant differences exist between the published nomograms of birth weight for gestational age. Differences in demographics, altitude above sea level and study methodology have all been implicated. A three-year experience of uncomplicated singleton gestations (N = 14,766) was analyzed for birth weight and gestational age. The mean, 10th percentile and their 95% confidence intervals were compared to Brenner's data and a pooled sample from other studies in this country. The mean birth weights were significantly higher than Brenner's after 32 weeks' gestation and higher than the pooled mean after 35. The 10th percentiles were significantly greater than Brenner's after 36 weeks' gestation. Approximately 66% of neonates at risk, below our 10th percentile, would not be identified with Brenner's criteria. We agree with Goldenberg that uniform criteria for the definition of the normal population are important in identifying aberrant growth. However, birth weight standards should be developed for the specific population in question.

Altitude

Microsphere estimates of blood flow: methodological considerations.

The microsphere technique is a standard method for measuring blood flow in experimental animals. Sporadic reports have appeared outlining the limitations of this method. In this study we have systematically assessed the effect of blood withdrawals for reference sampling, microsphere numbers, and anesthesia on blood flow estimates using radioactive microspheres in dogs. Experiments were performed on 18 conscious and 12 anesthetized dogs. Four blood flow estimates were performed over 120 min using 1 X 10(6) microspheres (15 microns) each time. The effects of excessive numbers of microspheres (13 million), pentobarbital sodium anesthesia (30 mg/kg), and replacement of volume loss for reference samples with dextran 70 were assessed. In both conscious and anesthetized dogs a progressive decrease in gastric mucosal blood flow and cardiac output was observed over 120 min. This was also observed in the pancreas in conscious dogs. The major factor responsible for these changes was the volume loss due to reference sample withdrawals. Replacement of the withdrawn blood with dextran 70 led to stable blood flows to all organs. The injection of excessive numbers of microspheres did not modify hemodynamics to a greater extent than did the injection of 4 million microspheres. Anesthesia exerted no influence on blood flow other than raising coronary flow. We conclude that although blood flow to the gastric mucosa and the pancreas is sensitive to the minor hemodynamic changes associated with the microsphere technique, replacement of volume loss for reference samples ensures stable blood flow to all organs over a 120-min period.

Animals

Estimating sexual behaviour parameters in the light of AIDS: a review of recent UK studies of young people.

This paper reviews UK surveys of the sexual behaviour of young people since the advent of AIDS. The studies, which are restricted to those whose samples were intended to be representative of the general population, reveal broadly similar estimates of key sexual behaviours (numbers with sexual experience, numbers of partners, frequency of intercourse, condom use and anal penetration). In spite of these similarities and the usefulness of such information for estimating the potential rate of transmission of HIV a number of methodological issues continue to afflict this kind of research. The paper calls for standardized question formats and a centralized body to monitor changes in these parameters of sexual behaviour.

Acquired Immunodeficiency Syndrome

Microbiological collaborative studies for quality control in food laboratories: reference material and evaluation of analyst's errors.

Working groups of the Federal Health Office in Germany and of the International Dairy Federation (IDF) have developed a quality assurance system to assess the analyst performance for colony count methods. The experiment design consists of several dilution series produced from a homogeneous sample suspension. Each series contains a number of twofold dilution steps with parallel plates on each level. The structure of this design permits a detailed analysis of the total variance and identification of analysts' methodological errors as single effects. A computer-based interpretation aid may classify the laboratory as working in correspondence with good laboratory practice ('acceptable') or, otherwise, furnishing 'to good' or 'unacceptable' results. Some examples are given for deviation depending on individual faults. Both, internal quality assurance and collaborative studies demand suitable reference samples. The reference material used has to meet certain requirements concerning homogeneity, contamination level and microbiological stability during a period of storage and transport. A naturally contaminated sample material was prepared, which has been used successfully in microbiological collaborative studies.

Animals

[Experiences with the Meyer-Probst encephalopathy questionnaire].

The validity of the questionnaire elaborated by Meyer-Probst for recording the typical behaviour of encephalopaths was checked on a sample comprising 254 high-risk and control children aged 6 to 7 years. The results confirm its value as a diagnostic aid in outpatient work and as methodological tool for research.

Attention Deficit Disorder with Hyperactivity

Liquid chromatographic determination of the antibiotic fumagillin in fish meat samples.

A procedure for the determination of fumagillin, an antibiotic of Aspergillus fumigatus in fish meat samples, using reversed-phase high-performance liquid chromatography is described. Liquid chromatography was performed on an octadecylsilane column using acetonitrile-water-phosphoric acid solution as mobile phase, with detection at 350 nm. Two different types of sample preparation were developed, clean-up and enrichment, and the limits of quantification were 100 ng/g and 5 ng/g, respectively, in fish meat. The recovery was 75 +/- 3% in the concentration range 100-500 ng/g. To introduce the methodology and demonstrate its usefulness, a practical experiment was performed. Trouts fed with fumagillin were examined for elimination of fumagillin. After 24 h, the concentration was shown to decrease to below 100 ng/g.

Animals

Gender differences in antidepressant and activating drug effects on self-perceptions.

Gender difference in neuropharmacologic responsivity have received surprisingly little attention in clinical research. In view of well-known gender differences in the prevalence of depression, we wondered whether men and women would respond differently to antidepressants or to an activational drug known to predict antidepressant response. Using clorgyline and D-amphetamine in 2 small samples, we illustrated several methodological approaches for examining possible gender-linked differences in the effects of these drugs on self-perceptions. Gender-linked differences in cognitive-perceptual processes may, in turn, be linked to differences in encoding or consolidating information about our experiences into our self-image for later recall.

Adult

Bringing social structure back into clinical decision making.

Although research in the past twenty years has resulted in an increasingly sophisticated understanding of clinical decision making processes, the dominant approach in this area of inquiry remains limited. Most studies emphasize normative models of how decisions ought to be made, others attempt to describe physicians' thinking, but few take the social context of decision making systematically into account. Research models typically assume that physicians are autonomous professionals practicing in socially insular clinical settings--an approach that is consistent with classic formulations of the social structure of medical practice, but they ignore 30 years of sociological research on research on patient-physician relationships and major historical changes in the structure of medical practice. Eisenberg's still timely advice to students of clinical decision making--that they need to describe decision making in the context of 'sociologic influences' (including patient, physician and practice setting characteristics)--is expanded in the present discussion. Recent studies are reviewed, highlighting important dimensions of social structure impinging on physicians' decision making. Findings indicate that the process of clinical decision making is likely influenced by patients' age, gender, socioeconomic status, and race, physicians' professional training and experience, as well as by larger structural features of organized clinical settings. Our review of these studies on the social context of clinical decision making, however, reveals major methodological limitations including those inherently imposed by secondary data analysis, normative approaches, written case vignettes, small, non-random samples and the inadequate control of confounding influences. We present a feasible, alternative research strategy, built on a factorial experimental design. Illustrative findings indicate how complex social structural influence on clinical decision making may be disentangled in an unconfounded manner.

Age Factors

Pethidine binding in plasma: effects of methodological variables.

Several methodological variables potentially influencing the plasma protein binding of [14C]-pethidine in vitro were investigated using equilibrium dialysis and rigorous pH control. Ionic strength of buffer, pethidine concentration and 21 days of plasma samples frozen to -8 degrees C did not affect the outcome of the binding experiments. Unbound fraction decreased with increasing temperatures between 25 degrees C and 37 degrees C. Unbound fraction decreased with increased pH between pH 7.0 and 8.0; the mean unbound fraction at an equilibrium pH of 7.4 and at 37 degrees C was 0.58 (s.d. 0.03, n = 58). It is likely that previous reports of pethidine unbound fraction as being between 0.2 and 0.4 represent artefacts caused by inadequate pH control during dialysis.

Blood Proteins

How large should my study be so that I can detect an altered sex ratio?

The world literature on manipulations purported to alter the gender of offspring is a quagmire full of reports presenting inadequate data and contradictory conclusions. Many are due to inappropriate statistical design or analysis. The authors present simple, nontechnical reference tables for determination of the minimum sample size necessary to detect a change in sex ratio (1) from a theoretical value, and (2) between two experimental populations. Simple methods for analyzing the results of gender manipulation experiments are described. Technical details are included in a separate section.

Female

A passive ozone sampler based on a reaction with nitrite.

Standard ozone monitoring techniques utilize large, heavy, and expensive instruments that are not easily adapted for personal or microenvironmental monitoring. For large-scale monitoring projects that examine spatial variations of a pollutant and human exposure assessments, passive sampling devices can provide the methodology to meet monitoring and statistical goals. Recently, we developed a coated filter for ozone collection that we used in a commercially available passive sampling device. Successful preliminary results merited further validation tests, which are presented in this report. The passive ozone sampler used in field and laboratory experiments consists of a badge clip supporting a barrel-shaped body that contains two coated glass fiber filters. The principle component of the coating is nitrite ion, which in the presence of ozone is oxidized to nitrate ion on the filter medium (NO2- + O3 produces NO3- + O2). After sample collection, the filters were extracted with ultrapure water and analyzed for nitrate ion by ion chromatography. The results from laboratory and field validation tests indicated excellent agreement between the passive method and standard ozone monitoring techniques. We determined that relative humidity (ranging from 10% to 80%) and temperature (ranging from 0 degrees C to 40 degrees C) at typical ambient ozone levels (40 to 100 parts per billion) do not influence sampler performance. Face velocity and sampler orientation with respect to wind direction were found to affect the sampler's collection rate of ozone. Using a protective cup, which acts as both a wind screen and a rain cover, we were able to obtain a constant collection rate over a wide range of wind speeds.

Environmental Exposure

The influence of depressive symptomatology on alcoholics' locus of control: a methodological note and a correction.

In a previously published experiment from our laboratory, we reported two findings. First, controls showed the expected negative correlation between internal and external LOC dimensions, while alcoholics did not. Second, a difference score between LOC-internal and LOC-external was correlated significantly and positively with alcoholics' abstracting impairment. Data from two new independent samples of alcoholics and controls confirmed the first finding, but not the second; further, a calculation error was discovered in the previously published data that invalidated the second result. However, in all three studies, depressive symptomatology was found to be correlated negatively with the internal-external LOC difference score in both alcoholics and controls. It is suggested that depressive symptomatology be assessed in any study that investigates LOC.

Adult

Mutagenic activity of disinfection by-products.

Data on raw water quality, disinfection treatment practices, and the resulting mutagenic properties of the treated water were compiled from pilot- and full-scale treatment experiments to evaluate that parameter which might produce variability in the results of a mutagenic study. Analysis of the data and comparison of treatment practices indicated that the measured mutagenic activity is strongly related to the characteristics of the organic matter in the raw water, the methodology used to sample and detect mutagens, the scale of the study both in terms of treatment flow and period of study, and the point at which and the conditions under which oxidants are added during treatment. Conclusions regarding disinfection systems in full-scale water treatment plants include the following: When raw water is pretreated and high concentrations of organics are present in the raw water, both ozonation and chlorination increased mutagenic activity. However, no significant difference in mutagenicity was found between the two oxidants. Both in the case of a nitrified groundwater and a clarified surface water, the mutagenic activity of the water after ozonation was related to its mutagenic activity before ozonation. With ozonation, mutagenic activity decreased after granular activated carbon (GAC) filtration. Thus, when GAC filtration follows ozone disinfection, early addition of oxidants may not be deleterious to the finished water quality. When chlorine or chlorine dioxide is added after GAC filtration, chlorine dioxide was found to produce a less mutagenic water than chlorine. Although these conclusions suggest means of controlling mutagenic activity during treatment, it must be stressed that the measurement of mutagenicity is a presumptive index of contamination level.

Carbon