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Forage of different physical forms in the diets of lactating Granadina goats: nutrient digestibility and milk production and composition.

To determine whether the energy balance of goats or characteristics of the diet consumed were the principal factors that determined milk production, feeding and digestion trials were carried out using two groups of 5 Granadina goats. The concentrate fraction of both diets was the same, but the forage fraction of the diets differed. In diet 1, the forage was in the form of long alfalfa hay, and, in diet 2, forage was in the form of pelleted alfalfa. Intake and the forage to concentrate ratio of the two diets were not significantly different, although diet 2 was more digestible. The amount of fat and protein in the milk depended on energy intake and not on dietary treatment. The milk protein of goats fed diet 2 was higher in casein. No sensible differences were noted in the fatty acid composition of the milk. Nitrogen and metabolizable energy utilization for milk production was greater for goats fed diet 2. According to the results obtained, it would seem advantageous to use pelleted alfalfa rather than alfalfa hay in the diets of goats.

Animal Nutritional Physiological Phenomena↗

Inference of Genetic Structure and the Process of Population Formation in Nepalese Native Goats Using Uniparental and Genome-Wide Markers.

Nepal is a small, landlocked country with marked elevational variation from the Terai plains to the Himalayas. Here, four indigenous goat populations (Chyangra, Sinhal, Khari, and Terai) are raised at different elevations. This study aimed to clarify the genetic structure of these populations and how they are formed and propagated across the Himalayan region. We analyzed 136 Nepalese goats using mitochondrial (mt) DNA D-loop and sex-determining region Y (SRY) 3'-untranslated region (UTR) sequences, as well as 50 K SNP array data. The mtDNA haplogroups D (0.162) and G (0.03) were detected only in Chyangra, whereas haplogroup B was predominant in Sinhal (0.42), followed by Khari (0.260). Regarding SRY haplotypes, Y2B was detected in all populations, whereas Y1AB (0.42) was found only in Chyangra. Genome-wide SNP analysis showed that Chyangra was genetically related to Tibetan and Central Asian goats, while Terai resembled South Asian goats. Interestingly, Sinhal formed a distinct cluster, whereas Khari exhibited an admixed genetic structure. These findings suggest that Nepalese goats originate from at least three ancestral lineages and that an additional migration route may have existed through the southern Himalayas.

50K SNP↗

The reversal of xylazine hydrochloride by yohimbine and 4-aminopyridine in goats.

Yohimbine, 4-aminopyridine, and a combination of the 2 drugs were studied to assess their potential as antagonists to xylazine in goats. Twenty-four small East African goats were divided randomly into 4 groups of 6 goats each in a placebo-controlled study. They were all treated with intramuscular xylazine at 0.44 mg/kg. At the time of maximum sedation, sterile water was administered intravenously to the control group, 0.15% 4-aminopyridine at 0.4mg/kg to Group 2, 0.1% yohimbine at 0.25 mg/kg to Group 3, and the combination of the 2 drugs at the same dose rates to Group 4. The yohimbine/4-aminopyridine combination was also used to antagonise xylazine at 0.88mg/kg in 6 goats. The heart rate, respiratory rate and rate of ruminal movements, the pedal and palpebral reflexes as well as the reaction to noxious stimuli, the standing time and the total recovery time were established and evaluated to assess the effects of the treatments. The drugs reversed the xylazine-induced decrease in the heart rate, respiratory rate and rate of ruminal movements, and also rapidly restored the reflexes as well as the reaction to noxious stimulation. In addition, they significantly (P < 0.05) decreased the mean standing time. The mean total recovery time was decreased significantly (P < 0.05) by 4-aminopyridine and the yohimbine/4-aminopyridine combination, but non-significantly (P > 0.05) by yohimbine. No relapse in sedation occurred. Overall, the combination of yohimbine and 4-aminopyridine produced better responses than the individual drugs, and may therefore be used for rapid reversal of xylazine-induced sedation in goats. Yohimbine or 4-aminopyridine may also be useful for this purpose but recovery may be prolonged.

4-Aminopyridine↗

Analgesic and systemic effects of xylazine, lidocaine and their combination after subarachnoid administration in goats.

The objective of the study was to determine the analgesic and systemic effects of subarachnoid administration of xylazine hydrochloride (XY), lidocaine hydrochloride (LI) and their combination (XYLI) in goats. Six healthy goats were used in a prospective randomised study. Three treatments were administered to each goat, with 1-week intervals between each treatment. Treatments consisted of 0.1 mg/kg xylazine, 2.5 mg/kg lidocaine and a combination of xylazine 0.05 (mg/kg) and lidocaine (1.25 mg/kg). Analgesia, ataxic, sedative, cardiovascular and respiratory effects, and rectal temperature were evaluated before (baseline) and at 5, 10, 15, and 30 min after subarachnoid injection, and then at 30-min intervals until loss of analgesia occurred. Lidocaine induced analgesia in 3.1 +/- 1 min (mean +/- SD), which lasted for 66 +/- 31 min. Heart and respiratory rates and blood pressure remained unchanged after lidocaine-induced analgesia. Xylazine induced analgesia in 9.5 +/- 2.6 min and xylazine-lidocaine in 3.2 +/- 1.2 min. Xylazine-lidocaine-induced analgesia lasted longer (178.3 +/- 37 min) than that induced by xylazine (88.3 +/- 15 min). The XYLI treatment induced prolonged motor blocking (115 min), more than the XY (80 min) and LI (90 min) treatments. Both xylazine and xylazine-lidocaine caused significant decreases in the heart and respiratory rates, but not in blood pressure. The combination of xylazine (0.05 mg/kg) and lidocaine (1.25 mg/kg) can be administered subarachnoidally (between last lumbar vertebra and 1st sacral vertebra) to produce prolonged (> 2.5 h) analgesia of the tail, perineum, hind limbs, flanks and caudodorsal rib areas in goats. Despite the prolonged analgesia, using this combination is desirable for relieving postoperative pain, but it may be a disadvantage due to a motor block when dealing with goats.

Adrenergic alpha-Agonists↗

Efficacy of UV light for the reduction of Listeria monocytogenes in goat's milk.

Certain types of goat's cheeses are produced using unpasteurized milk, which increases the food safety concerns for these types of products. Popularity and consumption of goat's milk products have increased, and the niche market includes gourmet goat's cheeses. The U.S. Code of Federal Regulations and the Pasteurized Milk Ordinance both address the possibility for processing alternatives to heat treatment, and the use of UV light treatment may be a viable alternative that still ensures the safety of the product. Fresh goat's milk was inoculated with Listeria monocytogenes (L-2289) at 10(7) CFU/ml and exposed to UV light using the CiderSure 3500 apparatus (FPE Inc., Macedon, NY). Inoculated milk was exposed to a UV dose range between 0 and 20 mJ/cm2 to determine the optimal UV dose. A greater than 5-log reduction was achieved (P < 0.0001) when the milk received a cumulative UV dose of 15.8 +/- 1.6 mJ/cm2. The results of this study indicate that UV irradiation could be used for the reduction of L. monocytogenes in goat's milk.

Animals↗

[Cell count and differential cell count in goat milk--variability and influencing factors].

In a total of 413 dairy goats of different breeds from five herds (milk performance 550-1000 kg) somatic cell counts and cell types were investigated in fore milk samples from udder halves, in composed udder and bulk milk samples. Cytoplasmatic particles in the milk were not counted. Three herds were investigated monthly during two years. At every sampling a clinical examination of the mammary gland (fore milk, teats, gland tissue, lymphatic nodes of the udders) and a bacteriological examination of the milk samples were carried out. In all herds the clinical health status of the udders was good. Independent of the sample type the mean SCC was about 1000 x 10(3)/ml with a wide range between several millions and < 100,000/ml. A seasonal influence on the monthly minima and maxima of the bulk milk SCC was evident, the lower SCC being recorded mainly during April to October. Increasing lactation numbers and months are connected with increasing SCC. Goats with high milk SCC at the begin of the lactation tended to maintain their high SCC levels as the lactation progressed. Limit values for SCC in goat milk should take into account the influence of the kidding season of the herds upon the dynamics of the bulk milk SCC. Polymorphonuclear leukocytes were the main cell type in goat milk (around 40%), their number tended to increase as total SCC increased, in particular in samples with cell counts above one million. Likewise, the differences between SCC in the milk of corresponding udder halves increased significantly as one of the halves showed more than one million somatic cells. Defining limit values for SCC in goat milk this should be taken into account.

Animals↗

[Serum gamma globulin concentration in goat kids after colostrum administration: effect of time of administration, volume and type of colostrum].

In this study, which was performed on a Dutch dairy goat farm, several aspects of the administration of colostrum to new-born goat kids were examined. Time of colostrum administration and amount and type of colostrum administered were compared. Effectiveness was measured as total serum protein content and gamma globulin fraction. No significant differences in serum gamma globulin titre were observed between kids that received colostrum at 30 or 60-90 minutes post partum, respectively. Titres were significantly lower in kids that received 100 ml of colostrum instead of 150-200 ml. The effect of sheep colostrum replacer or cow colostrum was also examined. Gamma globulin titres were significantly high with goat colostrum than with cow colostrum or sheep colostrum replacer, and titres were higher with cow colostrum than with sheep colostrum replacer. Based on the results of this experiment, the following protocol is suggested for colostrum administration to goat kids: single administration of 150-200 ml of goat colostrum within 90 minutes of birth. Use of cow colostrum is not advised because it may lead to transmission of paratuberculosis. Use of sheep colostrum replacer as a source of passive immunity is not recommended.

Animals↗

Prostaglandin F2 alpha-induced prolactin release and luteolysis in the goat.

Injection of prostaglandin F2 alpha (PGF2 alpha) initiated a significant increase in plasma prolactin levels in all goats except those in anoestrus. Luteolysis occurred in non-pregnant goats during the mid luteal phase when the goats were given PGF2 alpha either with or without the suppression of prolactin release by bromocryptine (CB154). Luteolysis and subsequent parturition also occurred in pregnant goats in mid and late gestation after PGF2 alpha injection, with an associated release of prolactin and decrease in plasma progesterone. Acute prolactin release in response to injection of thyrotrophin releasing factor may have had a transient effect on plasma progesterone levels, but did not appear to be luteolytic in either pregnant or non-pregnant goats.

Anestrus↗

[Serum gammaglobulin titer in goat kids after colostrum administration: effect of commercial colostrum replacers].

Seven commercially available colostrum replacers were tested for their ability to substitute for goat colostrum as a source of gammaglobulin for goat kids. One hundred kids were fed with goat colostrum or colostrum replacer after separation from their mothers at birth. Blood samples were taken from ten kids at birth, and from all kids at 48 hours after birth. Samples were used to measure the total protein content and gammaglobulin concentration of serum. Kids fed with goat colostrum had higher gammaglobulin concentrations than newborn kids or kids fed with replacer. For three replacers, the gammaglobulin concentrations were the same as those of newborn kids. For three other replacers, the gammablobulin concentrations were similar but higher than those of newborn kids. For one replacer, the levels of gammaglobulin were higher than those of in newborn kids and higher than those measured after feeding of the other replacers. None of the replacers was an adequate substitute for goat colostrum as a source of gammaglobulins.

Animal Feed↗

Depletion and bioavailability of imidocarb residues in sheep and goat tissues.

The residual depletion of a commercial product containing imidocarb dipropionate in sheep and goat tissues was investigated. Additionally, the oral bioavailability of residues was determined in rats to evaluate the extent to which tissue imidocarb residues could be reabsorbed by consumers. Ten ewes and 5 goats were administered im with a commercial formulation containing imidocarb dipropionate (Carbesia cavalli, Shering-Ploug 121.15 mg/ml) at the single dose of 3 mg/kg bw corresponding to 2.1 mg/kg bw imidocarb base. Two sheep and 1 goat were slaughtered 15, 30, 60, 90 or 120 d after dosing and samples of muscle, injection site muscle, liver, omental and subcutaneous fat, and kidneys were collected. Samples of cerebral hemisphere, cerebellum, olfactory bulb, pineal and pituitaryglands were dissected. For the residue bioavailability study 7 groups of3 Wistar rats each, were dosed by gavage with imidocarb dipropionate standard in water (group 2, 3 and 4) or with imidocarb as a liver residue collected from prior dosed animals (group 5, 6 and 7) at 8.4. 16.8 or 33.6 microg/kg of imidocarb base respectively, for 5 d. Group I was control. All animals were sacrificed the day after the last drug administration and livers were collected. The highest drug levels in sheep and goats occurred in liver and kidney, suggesting that these tissues are targets for residues; muscle had negligible importance as storage tissue. Goats had a lower storage capability than sheep. The residue profile in sheep liver and omental fat showed a 30-d storage period to reach maximum concentrations, and suggested that imidocarb is redistributed. The high and long-lasting concentrations in brain showed its capacity to cross the blood-brain barrier and caused concern for potential neurotoxic effects. Detectable concentrations of imidocarb were not found in rat liver.

Animals↗

[A study of the engraftment, expansion and differentiation of human hematopoietic stem cells in goats].

OBJECTIVE: To establish a human/goat hematopoietic stem cell (HSC) xenogeneic transplant model and to probe the engraftment, expansion and differentiation of human HSC in vivo. METHODS: Human HSCs were isolated from human umbilical cord blood and 1 x 10(5) human HSCs were in utero transplanted into 50 fetal goats at the 55 - 65 the gestation days. The engraftment, expansion and differentiation of human HSCs in goats were determined by FACS analysis, PCR and PCR-Southern blot hybridization at various intervals after birth. RESULTS: Hematopoietic chimerism occurred certainly in 35 of 39 live-born recipients. On average, The proportion of human hematopoietic cells in goat blood was 1% approximately 3% and remained phenotypically stable for at least 10 months. The human hematopoietic cells circulated in goat blood expressed CD34, CD14, CD20 and glycophorin A (GPA) but did not express CD3, CD4, CD7, CD8 and CD56 or expressed them at a very low level. CONCLUSION: The number of human HSC can be effectively expanded 1 000 - 10 000 fold. Human HSCs in goats undergo a limited differentiation. Our human/goat HSC xenogeneic model provides a useful way for the investigation of HSC transplantation, expansion and differentiation in vivo.

Animals↗

[Repair of tibial defect with tissue-engineered bone graft and radionuclide bone imaging in goats].

OBJECTIVE: To observe the effect of tissue-engineered bone grafts in repairing large tibial defect in goats, and assess the value of radionuclide bone imaging in monitoring the therapeutic effect of this approach. METHODS: Tibial defects measuring 2 cm was artificially made in the left tibia of 27 normal goats that were subsequently divided into 3 groups (9 each) to undergo treatment with tissue-engineered bone grafts, artificial bone grafts or without any grafts (as control group) respectively. The tissue-engineered bone grafts contained bone marrow stroma cells (BMSCs) of the goats and coral hydroxyapatite (CHAP), while the artificial bone grafts were from CHAP only. After the operations, radionuclide bone imaging was used to monitor the therapeutic effects at 2, 4 and 8 postoperative weeks. RESULTS: The 99mTc-MDP uptake of the region of interest (ROI) and the target to non-target ratios (T/NT) of the control group did not indicate any process of revascularization or bone regeneration. An increasing tendency of the revascularization and bone regeneration, in contrast, was observed in goats receiving the artificial bone grafts, a tendency that was far more obvious in the goats with tissue-engineered bone grafts. CONCLUSION: Tissue-engineered bone graft is eligible in repairing large defect in the caprine tibia, and radionuclide bone imaging may accurately monitor the revascularization and bone regeneration after the bone graft implantation.

Animals↗

[Ultrasonic measurement of the elastic properties of goat condyles].

OBJECTIVE: The purpose of this experiment is to investigate the elastic properties of goat mandibular condyles and to provide basic constants for biomechanical study of temporomandibular joint. METHODS: A fresh goat mandible was adopted and the standard condyle specimen of 4 mm x 6 mm x 10 mm was obtained from it. The structure density of the condyle was determined according to Archimede's principle. The transmitting velocities of ultrasonic wave in different directions of goat condyles were measured by using the ultrasonic system, which was composed of a transmitting transducer of 1.25 MHz, a receiving transducer of 2.5 MHz, an ultrasonic analyzer (5052UA) and a digital storage oscilloscope (HP54601A). The elastic coefficients and technical constants could be calculated by elastic equations. RESULTS: The structural density of the sample and the wave velocities in different directions of the condyle were both obtained by direct measurement. And then, nine elastic coefficients and all of the technical constants of orthotropic condyles were calculated. CONCLUSION: 1. It is practicable to measure elastic properties of the condyle by using ultrasonic technique. 2. Goat condyles of the mandible are a type of anisotropic and linearly elastic material. 3. The direction perpendicular to the transverse ridge of the goat condyle has the highest Young's modulus. As to shear modulus, the direction along the transverse ridge shows the highest value.

Animals↗

[Changes of biomechanical properties in goats at different times after ovariectomy].

This paper presents the changes of biomechanical properties in goats at different times after ovariectomy. 14 goats were randomly divided into three groups. These goats were ovariectomized and sacrificed at 180 days (n = 4), 360 days (n = 5) and 540 days (n = 5) after the operation. The right femur, tibia and metatarsus were taken out. The structure mechanical properties of femora were measured with the three-point bending method and those of metatarsus were measured with the compressing method. The material mechanical properties of flexure, compress and tensile strength in the compact bone specimens of femora and tibiae taken from broken ends were also measured. The results showed that the one-year group, compared with the half-year group, had all the bone geometric parameters decreased, but not statistically significant (P > 0.05); the elastic modulus of three-point bending of femora decreased by 23.27% and the others had no significant difference (P > 0.05); all the material parameters of flexurel, compress and tensile strength in compact bone specimens decreased, but only the ultimate strength of tibiae of flexure, compress and tensile strength decreased obviously (P < 0.05). The one and a half year group, compared with the one year group, had the bone density of femora and tibiae decreased, but not statistically significant (P > 0.05). All the bone geometric parameters of tibiae and the thickness of bone of femora increased (P < 0.05). The break load of three-point bending of femora increased (P < 0.05). Probably, it was related to the increase of the body weight in the one and a half year group. The elastic modulus and the ultimate strength decreased, but not statistically significant (P > 0.05). When compact bone specimens were used, the ultimate strength and elastic modulus of femora and tibiae in compress and the elastic module of tibiae in flexure decreased obviously (P < 0.05); and the others had no significant changes (P > 0.05). This study indicated that the degree of osteoporosis in ovariectomized goats increased at half a year, one year, and one and a half year postoperation, and hence suggested that the ovariectomized goat be a pretty useful big animal model of osteoporosis.

Animals↗

A prospective study on histochemical observation of thyroid gland at prepubertal Black Bengal goat.

In the present study, Period Acid Schiff (PAS), Verhoeff's and Van Gieson stains were used in the Department of Anatomy and Histology to observe the histochemical architecture of thyroid gland at prepubertal Black Bengal goat. Thyroid follicular cells were cuboidal in prepubertal goats. These cells showed moderate PAS-positive reaction in prepubertal group. PAS activity in the basement membrane of the thyroid follicles was mild in prepubertal Black Bengal goats. The lumen of the thyroid follicles contained homogenous intense PAS positive colloid materials in prepubertal goats. Parafollicular cells showed intense PAS positive reaction for glycogen in prepubertal goats. Capsule, connective tissue septa, interfollicular connective tissue of the thyroid gland mainly composed of collagen fiber. The wall of blood vessels in the capsule and interfollicular blood capillaries was composed of collagen fiber and elastic fiber. Elastic fiber formed the inner layer of the blood vessels showed blue black to black color. Inner layer of the capsule penetrated the gland parenchyma divided the gland into indistinct lobe and lobules. Collagen fiber distributed around the thyroid follicle formed the interfollicular stroma of the thyroid gland. The results of the present study can also be used to compare with that of human specially in AutoImmune Thyroiditis (AIT).

Animals↗

[Characteristic, composition, and cheese-making behavior of goat's milk].

Up until now, the composition of goat's milk has not been well known. However, every day it is more and more evident that this milk is quite different from cow's and other mammal's milk. This difference depends on a major concentration in some of milk elements. Caseins, in goat's milk are found in a minor concentration (their inner components have, besides, different relative proportions). On the other hand, non-proteinic nitrogen, calcium and phosphorus exist in a major concentration and biggest micellar size in goat's milk than in other milks. All these goat's milk qualities result in a different physicochemical composition which explains some of its characteristics and its very particular technological behaviour. They prevent, also, our associating goat's milk to cow's milk, as it has been done until recently.

Animals↗

[Pulmonary reaction after protamine reversal of heparin in goats and rabbits].

Protamine reversal of heparin is often associated with severe hemodynamic side-effects, including pulmonary hypertension in cardiovascular surgery. However, the precise mechanism of this transient pulmonary hypertension is not clear. Recently, it was reported that pulmonary intravascular macrophages (PIM) react avidly with and phagocytize various foreign particles (liposome, latex, red cells). Sheep, goat and pig have a large population of PIM, but rabbit, rat and human have few. To investigate whether the pulmonary reaction after protamine reversal is related to PIM, we compared the pulmonary reaction after protamine reversal between goats and rabbits. We also studied it in patients for cardiac surgery. Protamine sulphate (2 mg.kg-1) was injected in 2.5 min into femoral artery at five min after heparin sodium (200 IU.kg-1) injection in goats and rabbits. Mean pulmonary arterial and peak airway pressures increased significantly after protamine reversal in goat. On the other hand, in rabbit and human, mean pulmonary arterial and peak airway pressures showed no significant changes. Goat was far more sensitive to protamine reversal than rabbit and human. We conclude that PIM may be the main cause of the pulmonary reaction after protamine reversal.

Animals↗

Comparative aspects and sex differentiation of plasma sulfamethazine elimination and metabolite formation in rats, rabbits, dwarf goats, and cattle.

Plasma disposition and urinary recovery of sulfamethazine (SMZ), its N4-acetylated metabolite (N4AcSMZ), and 2 of its hydroxylated metabolites--5-hydroxysulfamethazine (5OHSMZ) and 6-hydroxymethylsulfamethazine (6CH2OHSMZ)--were determined in either sex of 4 animal species: rats, dwarf goats, rabbits, and cattle. Rats, rabbits, and dwarf goats had significant (P < 0.01) sex difference in SMZ plasma clearance. Male rats had higher plasma clearance than did female rats, and excreted higher amounts of the hydroxy metabolites and lower amounts of N4AcSMZ. The N4AcSMZ metabolite was predominant in plasma and urine of rabbits. Male rabbits had higher plasma clearance than did female rabbits, but differences in metabolite profile were not apparent. With regard to plasma SMZ elimination, the situation in goats was opposite to that in rats. Male goats had considerably lower clearance than did female goats. This was associated with a lower hydroxylation rat in males. Plasma half-life of SMZ in cows was lower than that in bulls, probably because of a smaller distribution volume in cows. Compared with elimination via urine, elimination via milk was negligible in cows. Significant differences in metabolite profiles were not found between bulls and cows. Similar to those in rats and mice, hormone-dependent xenobiotic metabolic pathways may exist in other species. Depending on species and xenobiotic compound residue concentrations of xenobiotics, their metabolites, or both may differ with sex of the animal, or may be altered after treatment with anabolic hormones.

Animals↗