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At least 1,099 records · Page 61Linked to original sources
PubMed1979

The major histocompatibility complex of Rhesus monkeys. XII: Cellular typing for D locus antigens in families.

Typing cells (TC) were used for the identification of D locus antigens in rhesus monkey families. The antigens defined by this cellular method always segregated with the other RhLA-controlled markers except in one offspring with a proven recombination within the RhLA region; in that case, the D antigen was inherited in coupling with an antigen of the Ia1 locus (the analog of the human DR locus). With two exceptions, each TC or group of TC's was associated with one of the serologically defined Ia1 antigens. The two exceptions were associated with "blanks" of that series. Interestingly, two independent (mutually MLC responsive) TC groups appeared to be associated with the same Ia1 antigen 75. Equally noteworthy was the observed "inclusion phenomenon" among cells of several TC groups, each associated with a single Ia1 antigen or blank of that series. In these cases, some of the TC's of the group were unidirectionally MLC-responsive against other cells of the same group. Certain pitfalls of cellular typing are discussed; unexpected "false positive" results were attributed to prior immunization with products of non-D loci which have described recently (van Es & Balner 1978b).

Animals↗
PubMed1981

Pooled sera for cytotoxicity testing: accurate, time- and cost-effective preliminary crossmatches.

To conserve sera, reduce technologist time and facilitate distribution of kidneys to the most likely recipient from groups of highly sensitized patients, we studied the feasibility of using pooled sera in preliminary crossmatches. From 16 chronic renal failure patients, 275 sera were studied against fresh cell panels from 24 to 35 donors. Using the antiglobulin crossmatch technique, sera were studied individually, in consecutive pools of five and in consecutive pools of 10. Reactions of component sera were assessed on a cell-by-cell comparison with their pools of five and 10 sera. Crossmatches performed with pools of five sera and pools of 10 sera detected the same cytotoxic reactions as did their component sera in 97.6% and 97.8% of the reactions, respectively. We conclude that 10 sera can be pooled so that preliminary crossmatch testing of all positive sera of all theoretically potential allograft recipients can be accomplished with accuracy and efficiency, even in the larger regional distribution centers.

Antilymphocyte Serum↗
PubMed2006

HLA-B27 testing in Thai patients using the PCR-SSP technique.

We develop the HLA-B27 test kit using the PCR-SSP technique. Five hundred forty blood samples were tested for HLA-B27 by microlymphocytotoxicity test (LCT) and PCR-SSP. It was found that 127 (23.5%) and 134 (24.8%) of these samples were positive for HLA-B27 by LCT and PCR-SSP, respectively. The sensitivity and specificity of the PCR-SSP were 94.8 and 100%, respectively, when using LCT as the standard method. The PCR-SSP positive predictive value was 100%, negative predictive value was 98.3%, and a concordance rate of 98.7%. This study shows that the PCR-SSP is simple, convenient, and a more cost-effective in-house test kit.

Cytotoxicity Tests, Immunologic↗
PubMed1980

Platelet HLA typing and cross-matching by a direct microcytotoxic assay. A new method employing carboxyfluoresceindiacetate-stained platelets.

Carboxyfluoresceindiacetate-labelled platelets can be used in direct microcytotoxic assays for the purpose of typing platelets for a number of antigenic determinants, such as HLA and beta-2 microglobulin. The assay when used in direct platelet cross-matching, where thrombocytopenic patient sera were screened, resulted in the uncovering of many extrapositive and weakly positive cross-matches not identified by the lymphocyte cross-match. The simplicity and rapidity of this new method makes it an attractive alternative for future use in research and clinical studies.

ABO Blood-Group System↗
PubMed1994

CD4 and CD8 monoclonal antibody therapy in canine renal allografts.

Therapy with CD4 and CD8 monoclonal antibodies was evaluated in dogs which received double-haplotype MHC-mismatched renal allografts. Neither CD4 nor CD8 monoclonal antibodies given alone prolonged allografts survival (creatinine > or = 300 micromol/l) beyond 7 days. However, combined therapy with CD4 and CD8 antibodies given up to day 10 did prolong allograft survival to a median of 14 days. A longer (21 day) course of CD4 and CD8 antibodies did not extend allograft survival further. The effect of prolonged antibody therapy was restricted by the occurrence of both an antiglobulin response and an anaphylactoid reaction to the monoclonal antibody preparation. When the CD4 and CD8 antibodies were combined with a pan-T-cell-depleting Thy-1 antibody, the survival of double-haplotype mismatched allografts was further prolonged (median 16 days). The median survival of single-haplotype mismatched renal allografts on this triple therapy was 21 days, with one surviving to day 36.

Anaphylaxis↗
PubMed1998

The minor histocompatibility antigen HA-1: a diallelic gene with a single amino acid polymorphism.

The minor histocompatibility antigen (mHag) HA-1 is the only known mHag for which mismatching is correlated with the development of severe graft versus host disease (GvHD) after human leukocyte antigen-identical bone marrow transplantation. HA-1 was found to be a nonapeptide derived from an allele of the KIAA0223 gene. The HA-1-negative allelic counterpart encoded by KIAA0223 had one amino acid difference from HA-1. Family analysis with HA-1 allele-specific polymerase chain reaction showed an exact correlation between this allelic polymorphism and the HA-1 phenotype. HA-1 allele typing of donor and recipient should improve donor selection and allow the determination of bone marrow transplantation recipients with high risk for HA-1-induced GvHD development.

Alleles↗
PubMed2003

The role of minor histocompatibility alloantigens in penetrating keratoplasty.

BACKGROUND/AIMS: To review the experimental evidence that implicates minor histocompatibility (H) antigens in the vulnerability of orthotopic corneal transplants to rejection, and to speculate on the possible role of minor H antigens in penetrating keratoplasty in humans. METHODS: Orthotopic corneal transplantations have been conducted in numerous inbred strains of mice and rats, and the fate of these grafts, as well as the immune responses mounted by recipients, have been examined in vivo and in vitro. RESULTS: Minor H antigens have been found to be more important barriers to the success of orthotopic corneal transplants in rodents than have grafts disparate at the major histocompatibility complex (MHC). The reasons for this reversal of the immunogenetic rules of transplantation reflect unique features of the cornea with respect to (a) expression of MHC-encoded molecules, (b) content of class II MHC-bearing antigen presenting cells, (c) vulnerability to rejection by direct alloreactive T cells, and (d) existence of ocular immune privilege. CONCLUSION: Since minor H antigens are the major barriers to acceptance of orthotopic corneal allografts in rodents, minor H antigens should be considered as important targets of rejection in failed penetrating keratoplasty in humans.

Animals↗
PubMedDate not supplied

HLA antigens in five Amerindian groups (Yuko, Bari, Tunebo, Guane and Paez) of Colombia: results of 'Expedición Humana'.

The serological HLA types (A, B, C and D loci) were studied in five different Indian groups in Colombia. The range of polymorphism was not very restricted in these groups, but there was significant genetic heterogeneity among the five populations in all the HLA loci. The gene frequency data, when converted into a kinship matrix and a two-dimensional eigen vector plot, showed a closer affinity between Bari and Yuko Indians, while Guane, Tunebo and Paez Indians were not only genetically different from the former but also well-differentiated from each other. It seems therefore from this study that geographical proximity may play a greater role than linguistic similarities in the genetic affinities of Colombian Amerindians.

Antigenic Variation↗