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PubMedDate not supplied

Identification of rib cage's lower margin in cardiopulmonary resuscitation.

The Guidelines of the American Heart Association (J Am Med Assoc 1992;268(16):2184-2198) and the European Resuscitation Council (Resuscitation 1992;24:103-110; Resuscitation 1998;37:67-80) for adult basic cardiopulmonary resuscitation (CPR), suggest that the hands of the rescuer should be positioned on the sternum of the victim after identification of the lower margin of the rib cage, but how this identification is made is not described. Lay persons have problems with the identification of anatomical structures that they cannot actually see (Bahr et al., Resuscitation 1997;35:23-6). Although this does not present a problem for those familiar with human anatomy, we have noticed that lay persons have problems with the identification of the lower margin of the rib cage. In this report, we suggest a method for correct and rapid recognition of the costal margin.

Cardiopulmonary Resuscitation↗

PubMed2003

Development of specific fluorescent oligonucleotide probes for in situ identification of wine lactic acid bacteria.

A rapid method for the identification of lactic acid bacteria (LAB) from wine has been developed. This method is based on fluorescence in situ hybridisation (FISH), using fluorescent oligonucleotide probes, homologous to 16S rDNA of those species of LAB commonly found in wines. The protocol for the specific detection of these bacteria was established through the hybridisation of 36 reference strains. The specificity of the probes was evaluated by using pure cultures. Probes were used to identify species in different wines, making it evident that direct identification and quantification from natural samples without culturing is also possible. The results show that FISH is a promising technique for the rapid identification of LAB, allowing positive identification in a few hours (4-16 h).

Base Sequence↗

PubMed1997

Identification of Clostridium chauvoei in cultures and clinical material from blackleg using PCR.

An identification system for Clostridium chauvoei, using PCR amplification of the 16S rRNA gene (rrs) with specific oligonucleotide primers and subsequent restriction digestion of the amplification product is described. The specific oligonucleotide primers were designed based on the rrs gene sequences of C. chauvoei by comparing it to the DNA sequences of the rrs genes of its most closely related species Clostridium septicum and Clostridium carnis. A subsequent restriction digestion of the 960 bp amplification product was used in order to unambiguously identify C. chauvoei. The developed identification system was evaluated on clinical material during a recent outbreak of blackleg in cattle. Thereby, C. chauvoei was identified as the etiologic agent of the outbreak either directly from clinical samples of muscle, liver, spleen and kidney or from primary cultures made with this material. A comparison of the newly developed method with standard diagnostic tools for C. chauvoei showed that it has advantages over the immunofluorescence and is, therefore, a useful option to it. Moreover, the assay is a valuable tool for the phylogenetic identification of C. chauvoei which can assist to substitute the fastidious traditional identification methods and replace laboratory animal testing currently used.

Animals↗

PubMed2001

Temporal envelope expansion of speech in noise for normal-hearing and hearing-impaired listeners: effects on identification performance and response times.

The effects of expanding the temporal envelope of speech sounds on speech identification in noise were investigated in normal-hearing and hearing-impaired listeners. Expansion was performed by applying a power-law transformation to the low-frequency temporal modulations (<500 Hz) of vowel-consonant-vowel logatomes presented against a background noise. Stimuli were spectrally degraded, allowing a direct examination of the perceptual effects induced by the modification of the temporal envelope alone on speech reception. This study extended a previous study conducted by Lorenzi et al. [1999. Hear. Res. 136, 131--138] by measuring the effects of envelope expansion on both identification performance and response times in normal-hearing and hearing-impaired listeners. Overall, the results show that temporal expansion yields only small improvements in identification scores (approximately 5%) in normal-hearing listeners. No effect of expansion on identification scores was observed in hearing-impaired listeners. On the other hand, the results show that expansion led to a significant decrease in response times in both normal-hearing and hearing-impaired listeners. The average benefit from expansion was about 65 ms in both groups. These results suggest that expanding the temporal envelope of speech sounds presented in noise may improve 'ease of listening' in both normal-hearing and hearing-impaired listeners.

Adult↗

PubMed2003

Messenger RNA profiling: a prototype method to supplant conventional methods for body fluid identification.

Conventional methods of body fluid identification use a variety of labor-intensive, technologically diverse techniques that are performed in a series, not parallel, manner and are costly in terms of time and sample. Theoretically, the identification of a body fluid may be made by determining a sufficient number of mRNAs that are expressed exclusively in cells that collectively comprise that body fluid. Advantages of an mRNA-based approach, compared to conventional biochemical methods of analysis, include greater specificity, simultaneous and semi-automatic analysis through a common assay format, improved timeliness, decreased sample consumption and compatibility with DNA extraction methodologies. In this report, we demonstrate that RNA is stable in biological stains and can be recovered in sufficient quantity and quality for analysis. Messenger RNA from the housekeeping genes S15, beta-actin and GAPDH was detected in blood, semen and saliva stains using a sensitive reverse transcriptase-polymerase chain reaction assay (RT-PCR). Additionally, we have identified a number of candidate tissue-specific genes, statherin, histatin 3, PRB1, PRB2 and PRB3 that may be useful for the positive identification of saliva. Messenger RNAs from these genes were detectable in saliva stains but not in blood or semen stains. Collectively these findings constitute the basis of a prototype RNA based assay system that may eventually supplant conventional methods for body fluid identification.

Actins↗

PubMed1997

Human identification by genotyping of personal articles.

In cases of personal identification of unknown skeletal remains, forensic scientists normally analyze an individual's genotype or phenotype by comparison with those of other family members such as patients. However, such genotyping does not necessarily provide conclusive proof of identity. In this study, we attempted personal identification by comparison of several short tandem repeat (STR) loci in samples from three corpses in various stages of decomposition with samples from personal articles of the respective individuals. The first victim had been found dead in a burned car. Almost 100% of his body was charred. The genotypes of the iliac bone from the burned body with regard to several STR loci were compared with those from a single hair recovered from the vacuum cleaner from the candidate's home. The second victim was found in a forest after more than 5 years. Several STR loci genotypes of skeletal bone were compared with those of the candidate's mummified umbilical cord kept in his parent's house. In the last case the victim was found in the sea after more than 7 months. The genotypes of the loci in the pubic bone of the corpse were compared with those of a menstrual bloodstain on the candidate's underwear left in the washing machine at her home. We succeeded in identification of the three cases by comparison of several VNTR and STR loci in the corpses with those in personal articles. In conclusion, personal identification can sometimes be performed more precisely by genotyping of personal articles than by comparison with the genotypes of relatives.

Adult↗

PubMed2000

Simultaneous identification of five bifidobacterium species isolated from human beings using multiple PCR primers.

On the basis of 16S rRNA sequences, 5 species-specific forward primers were designed for the identification of 5 Bifidobacterium species isolated from human intestine, namely B. bifidum, B. adolescentis, B. infantis, B. breve and B. longum. As the 5 primers targeted at different sites of 16S rDNA, by using their mixture and a genus-specific reversed primer, the 5 Bifidobacterium species can be simultaneously identified in individual or in mixed culture through PCR amplification. The specificity of the primers was confirmed by the use of genomic DNAs from type strains of all 32 Bifidobacterium species and 6 other relatives. The 5-primer mixture was also applied to the identification of Bifidobacterium strains used commercially. The results turned out to be in accordance with those from conventional identification. This multiple-primer method provides a useful tool for rapid identification of the 5 Bifidobacterium species indicated.

Bifidobacterium↗

PubMed1998

Identification of classical Propionibacterium species using 16S rDNA-restriction fragment length polymorphisms.

The phenotypic identification of the classical propionibacteria is essentially still problematic and alternative techniques for the identification of the various species are required. A rapid and sensitive technique for the routine identification of the classical propionibacteria, based on the amplification of 16S rRNA genes using the polymerase chain reaction and the subsequent restriction endonuclease digestion of the PCR products, was previously described. Although this technique enabled differentiation between the various classical species examined it was only evaluated on a limited number of type and reference strains. During this study, the taxonomic relationship between 135 Propionibacterium strains from diverse ecological niches, representing four classical species was investigated using this PCR/RFLP technique. Visual differentiation between the classical Propionibacterium was possible after restriction endonuclease digestion of the PCR products obtained using primers 16sP1-16sP4 and 16sP3-16sP4 with the restriction endonucleases HaeIII, AluI and HpaIII, respectively. With the exception of strains independently identified as "P. rubrum" and "P. sanguineum", the results of this study confirm the consolidation of the "old" species into the various classical species as they currently exist. It was therefore concluded that the PCR/RFLP protocol is suitable for the rapid and routine identification of the classical propionibacteria.

Bacterial Typing Techniques↗

PubMed1997

Identification of drugs in pharmaceutical dosage forms by X-ray powder diffractometry.

A simple X-ray powder diffractometric (XRD) method was developed for the identification of the active ingredient in a variety of dosage forms. The method was successfully used to unambiguously identify the active ingredient(s) in tablet, capsule, suppository and ointment formulations. The unique feature of the method is that it provides information about the solid-state of the drug. Thus, a capsule formulation containing anhydrous ampicillin was readily distinguished from that containing ampicillin trihydrate. The USP stipulates the use of the beta-polymorphic form of anhydrous carbamazepine in carbamazepine tablets. Contamination by the alpha-polymorph (down to a level of 1.4% w/w of the formulation) could be detected. In some of the multicomponent formulations, there was a pronounced overlap of the powder patterns of ingredients which made identification difficult. This problem was solved by using a pattern subtraction technique, which permitted selective subtraction of the XRD pattern of the constituents of the formulation from the overall XRD pattern. Such an approach enabled identification of the drug even when it constituted only 5% w/w of the formulation. The method also permitted simultaneous identification of the multiple active ingredients in trimethoprim-sulfamethoxazole and acetaminophen-aspirin-caffeine formulations.

Capsules↗

PubMed2001

Rapid identification of pathogenic bacteria by single-enzyme amplified fragment length polymorphism analysis.

Despite major progress in their treatment and prevention, bacterial infections remain a significant cause of morbidity and mortality worldwide. In responding to a disease outbreak, rapid and accurate identification of the bacterial species involved is of paramount importance. Strain level discrimination is desirable to allow selection of treatment modalities, and in the case of a deliberate release, for identification of the source. Single-enzyme amplified fragment length polymorphism (SE-AFLP) analysis was used to perform species and strain identification of subgroup I Bacilli, Yersinia, Staphylococci and Escherichia coli. By careful selection of AFLP primers, it was possible to obtain reproducible and sensitive identification to strain level, even within the highly monomorphic species Bacillus anthracis. SE-AFLP fragments can be analyzed using standard gel electrophoresis, and can be easily scored by visual inspection, due to the low complexity of the fingerprint obtained by this method. These features make SE-AFLP suitable for use in either field or laboratory applications.

Bacillus↗

PubMed2001

Evaluation of an automated system for the identification and antimicrobial susceptibility testing of enterococci.

The performance of a new version of an automated system panel, the Positive Combo Panel Type 11 of MicroScan WalkAway 96 (WA96; Dade Behring) was evaluated and compared to that of reference methods for the identification and for antimicrobial susceptibility testing of the different enterococcal species. A total of 376 enterococcal isolates were tested. The MicroScan WA96 correctly identified 99.6% (266/267), 78.3% (18/23) and 68.6% (59/86) of Enterococcus faecalis, Enterococcus faecium and species other than E. faecalis and E. faecium, respectively. Although low probability of accurate identification was obtained for 37 (9.8%) strains, the system indicated that supplementary tests were necessary for precise identification of 8 (9.3%) among the 86 strains included in the non-faecalis/non-faecium group and of 3 (13.0%) among the E. faecium isolates. In comparison to the agar screening method, the percentage of agreement for detection of resistance markers by the automated system was 90.2% (37/41) for ampicillin, 90.6% (48/53) for high-level resistance to streptomycin (HLRS), 96.4% (80/83) for high-level resistance to gentamicin (HLRG), and 100% (14/14) for vancomycin. The results indicate that the MicroScan WA96 performed well for the identification of E. faecalis and typical E. faecium isolates, and for the detection of resistance to vancomycin and HLRG. However, the system still needs further improvement in order to provide reliable results for the characterization of the other enterococcal species, including atypical variants of E. faecium.

Autoanalysis↗

PubMed1998

Evaluation of CHROMagar Candida for presumptive identification of clinically important Candida species.

CHROMagar Candida is a recently described and differential medium for the isolation and the presumptive identification of clinically important yeasts. We evaluated it with 262 yeast strains from clinical specimens, including 173 Candida albicans, 21 Candida tropicalis, 8 Candida krusei, 49 Candida glabrata, and 12 strains of other yeast species. Strains were presumptively identified on the basis of colony color and texture. These observations were compared with conventional identification results. Candida albicans was identified correctly in 170 (98%) of the 173 strains. A total of 46 of the 205 specimens that were plated on CHROMagar contained mixed cultures of yeast. Thirty-seven (80%) of these mixed cultures were not detected in the original specimens. CHROMagar Candida was useful for the rapid presumptive identification of Candida albicans and facilitated the recognition of mixed cultures. For other yeast species, it may provide additional information to laboratories that do not regularly perform identifications beyond the germ tube test.

Candida albicans↗

PubMed1999

Practical approach to the identification of clinically relevant Enterococcus species.

Enterococci have become important nosocomial pathogens, with Enterococcus faecalis and then Enterococcus faecium predominating. Because of the emergence of glycopeptide (vancomycin and teicoplanin) resistance in enterococci, laboratories have been required to screen for resistant strains and to identify them to the species level. This has resulted in the need for accurate identification of species less commonly associated with clinical infections, such as Enterococcus casseliflavus and Enterococcus gallinarum, which are inherently resistant to the glycopeptides. Studies evaluating commonly used commercial identification systems, have found error rates for enterococcal species identification of 2-21% for E. faecalis, 5-9% for E. faecium, and 14-79% for other species. Reporting errors may have adverse effects on the management of clinical infections, as well as in the control of multidrug-resistant strain outbreaks. The purpose of this document is to present a simplified approach to the identification of Enterococcus species that uses a combination of rapid, readily available, and inexpensive tests.

Anti-Bacterial Agents↗

PubMed1999

Novel microtiter plate format for testing germ tube formation and proposal of a cost-effective scheme for yeast identification in a clinical laboratory.

The germ tube test is most widely used for presumptive identification of Candida albicans. Conventional testing is relatively time-consuming due to the hands-on time involved in preparing and viewing each isolate. In order to reduce work-load and costs we have developed a novel microtiter plate test format that offers several advantages: (i) use of removable strips of microtiter wells placed in lockwell frames, (ii) only one pipetting step for each isolate, (iii) direct micromorphological evaluation using an inverse microscope, (iv) use of a novel synthetic germ tube test medium, (v) reduction of the inoculum, permitting testing of minute colonies, (vi) thus, testing of different colonies in potentially mixed primary cultures of clinical specimens is encouraged and facilitated. Implementing this microtiter based germ tube test with simultaneous trehalase test for presumptive identification of Torulopsis (Candida) glabrata, we propose an identification scheme including this test format. This has been implemented in our routine laboratory permitting cost-effective presumptive identification of almost all clinically relevant yeast species.

Candida↗

PubMed2000

Rapid identification of clinical yeast isolates using the colorimetric AUXACOLOR system.

The AUXACOLOR colorimetric system (Sanofi Diagnostics Pasteur, Marnes-la-Coquette, France) for the identification of clinical yeast isolates, was compared in its identification of 100 yeast strains to conventional identification methods. Of the 94 correctly identified isolates, 47% (n = 44) were identified by 24 h, and 100% (n = 94) were identified by 48 h. AUXACOLOR is a simple, rapid and accurate method for the identification of yeast pathogens.

Antifungal Agents↗

PubMed1986

Noninvasive identification of left main and triple vessel coronary artery disease: improved accuracy using quantitative analysis of regional myocardial stress distribution and washout of thallium-201.

The capabilities of visual and quantitative analysis of stress redistribution thallium-201 scintigrams, exercise electrocardiography and exercise blood pressure response were compared for correct identification of extensive coronary disease, defined as left main or triple vessel coronary artery disease, or both (50% or more luminal diameter coronary narrowing), in 105 consecutive patients with suspected coronary artery disease. Extensive disease was present in 56 patients and the remaining 49 had either less extensive coronary artery disease (n = 34) or normal coronary arteriograms (n = 15). Although exercise blood pressure response, exercise electrocardiography and visual thallium-201 analysis were highly specific (98, 88 and 96%, respectively), they were insensitive for identification of patients with extensive disease (14, 45 and 16%, respectively). Quantitative thallium-201 analysis significantly improved the sensitivity of visual thallium-201 analysis for identification of patients with extensive disease (from 16 to 63%, p less than 0.001) without a significant loss of specificity (96 versus 86%, p = NS). Eighteen (64%) of the 28 patients who were misclassified by visual analysis as having less extensive disease were correctly classified as having extensive disease by virtue of quantitative analysis of regional myocardial thallium-201 washout. When the results of quantitative thallium-201 analysis were combined with those of blood pressure and electrocardiographic response to exercise, the sensitivity and specificity for identification of patients with extensive disease was 86 and 76%, respectively, and the highest overall accuracy (0.82) was obtained.(ABSTRACT TRUNCATED AT 250 WORDS)

Adult↗

PubMed1997

Detection of "functional" valves in reversed saphenous vein bypass grafts: identification with duplex ultrasonography.

PURPOSE: Although venous valve lesions have been cited as a mechanism of graft failure, the fate of venous valves in reversed saphenous vein (RSV) bypass grafts is unclear. The basis for this uncertainty is the difficulty in postoperative identification and follow-up of valve sites and the infrequent pathologic submission of vein graft lesions. This report describes the features of "functional" valves (FV) visualized in RSV grafts by ultrasonic duplex scanning. METHODS: Sixty-six RSV infrainguinal vein grafts were prospectively studied with duplex ultrasonography from January 1992 to December 1995. Grafts were studied at 1, 2, 3, 4, 6, 9, 12, 18, and 24 months, then annually. FV identification was based on a characteristic ultrasound Doppler waveform and color flow pattern and visualization of the leaflets by B-mode imaging. The waveform consists of end-systolic valve closure followed by variable degrees of reflux. Immediate postoperative reactive hyperemia precludes detection, because flow reversal in the graft is needed for identification. RESULTS: Since August 1994, 14 FV have been identified in 11 (17%) of 66 RSV grafts. The mean time to FV recognition after implantation was 10 months (range, 1 to 52 months), and the average follow-up was 15 months. One valve was completely competent. Seven (50%) of the FV were associated with the development of a < 50% diameter reducing stenosis by Doppler velocity criteria. None of the FV has required intervention. CONCLUSIONS: "Functional" vein valves in RSV grafts are common and can be identified by ultrasonic duplex imaging. Awareness of the characteristics of FV during routine duplex graft surveillance will undoubtedly increase detection. The variable time course to identification of FV and duration of "function" warrants continued follow-up to determine the relationship of these valves to the development of stenotic lesions and graft failure.

Aged↗

PubMed2002

Patient age affects identification rate of sentinel nodes in breast cancer.

BACKGROUND: The aim of this study was to investigate whether clinicopathological features affect the success rate of identifying sentinel nodes using a combination method of dye and radioisotope in breast cancer patients. METHODS: Sentinel node biopsy was performed in patients (n = 154) with stage I and II breast cancer using a combination method. The association between the clinicopathological features of breast cancer and the success rate in the identification of sentinel nodes was investigated. RESULTS: Sentinel nodes were successfully identified in 147 (95.5%) of the 154 patients. There was a concordance between the sentinel-node and axillary-node status in 146 (99.3%) of 147 cases. The false negative rate of the sentinel node biopsy was 2.4% (1/42). The identification rate of sentinel nodes was significantly (P = 0.005) lower in patients > or = 60 years (84.4%) than in those < 60 years (98.4%). The tumor size, histological node status, lympo-vascular invasion, history of previous surgical biopsy, operative procedure, tumor location, and histological type were not related with the identification rate of sentinel nodes. The mean count ratio of all hot nodes was 14.0 (range 2.0-73.6) in patients > or = 60 years and 73.4 (2.0-1700.6) in patients < 60 years, and this difference was statistically significant (P < 0.0001). In addition, the mean number of sentinel nodes was significantly (P = 0.0006) lower in patients > or = 60 years (1.3; range one to four) than in patients < 60 years (2.1; range one to 12). CONCLUSIONS: Patient age affects the identification rate of sentinel nodes using a combination method in breast cancer.

Adult↗