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PubMed1986

Esterase activity in the bovine sweat gland: genetic differences and the effect of temperature.

The esterase activity in the sweat glands of Brahman (B), Brahman crossbred (BX) and Shorthorn (S) steers was studied in animals exposed to low heat loads while grazing. The percentage of sweat glands containing esterase in the secretory cells of the fundus differed between genotypes and ranked in order of their heat tolerance, i.e., B greater than BX greater than S. Esterase activity in some of those secretory cells was reduced in B bulls by acute exposure to high air temperatures in a climate chamber. While the significance of the esterase-containing cells in the fundus of the bovine sweat gland is uncertain, the results suggest that they could represent the resting or relatively inactive stage of the secretory cells.

Animals↗

PubMed1987

Sweat pore count, hair density and tooth size: heritability and genetic correlation.

In 109 subjects from 50 families without major inherited disorders, sweat pore count showed the anticipated reduction with age but also unexpected systematic differences between sides and between fingers. After adjustment for age and sex, significant genetic variation was found for sweat pore count, hair density and tooth size. The three traits were not phenotypically correlated but there was a significant negative genetic correlation between hair density and sweat pore count. The findings suggest that the same genes influence these two traits in opposite directions and that genetic and environmental influences may act on them through different physiological mechanisms.

Age Factors↗

PubMed1978

Sweating treatment for chronic renal failure.

Sweating was induced in 3 uremic patients in an ordinary bathtub for 3 h per day to examine whether clinically significant amounts of urea could be removed. Blood urea concentrations fell in all 3 patients, and 2 of the patients had improvement in uremic syptoms. Clearances of urea by a forearm collection technique in 2 patients were 20.9 +/- 3.7 and 11.6 +/- 3.9 ml/min. Average sweat volumes were 813 +/- 62 and 566 +/- 160 ml/h. Sodium concentrations were 52 +/- 47 and 76 +/- 12 mEq/l. This removal of urea, water and salt suggests that sweating could be used to treat uremia in conjunction with charcoal hemoperfusion, in patients awaiting vascular access, or during the interdialytic interval in patients with problems with overhydration.

Adult↗

PubMed1993

Sweat studies under conditions of moderate heat stress in two patients with the Nägeli-Franceschetti-Jadassohn syndrome.

The sweating response of 2 patients with the Nägeli-Franceschetti-Jadassohn syndrome was measured by direct calorimetry. During the experiment, under conditions of moderate heat stress, the 2 patients felt comfortable and showed a normal thermoregulatory response. This tended to corroborate the patients' history which did not reveal a clear-cut heat intolerance. However, the starch-iodine test on the right flexor forearm revealed a marked reduction in activated sweat gland density while the patients were still under experimental conditions. Probably this was not the only region with a reduced number of functioning glands. Still, the patients' overall performance through the experiment indicated that their total number of functioning glands was able to produce a sweat output sufficient to prevent disturbances in thermoregulation. To our knowledge, this is the first study of the thermoregulatory capacity of patients suffering from a hereditary dermatosis in which hypohidrosis is part of the clinical picture.

Adult↗

PubMedDate not supplied

Cytologic features of pleural effusion in apocrine sweat gland carcinoma. A case report.

BACKGROUND: Carcinoma arising in the apocrine sweat glands is very rare, and there are few reports of the cytologic features. We encountered a case of metastatic apocrine carcinoma in a pleural effusion. CASE: A 46-year-old male had a dark reddish nodule in the right axillary region that was diagnosed as apocrine carcinoma of skin appendage origin. Three years after wide resection and chemotherapy, widespread metastases developed with a massive pleural effusion. Needle aspiration fluid cytology contained clusters of adenocarcinoma. Some tumor cells had abundant cytoplasm or periodic acid-Schiff-positive, coarse granules. Decapitation secretion was occasionally found on the cell surface. Immunohistochemically, the tumor cells were often positive for BRST-2 and BRST-3. CONCLUSION: Cytologic features of metastatic apocrine sweat gland carcinoma show some characteristics of adenocarcinoma. Moreover, its definitive diagnosis in a pleural effusion can be made because of retaining the characteristics of apocrine sweat gland.

Apocrine Glands↗

PubMed1970

Metabolic studies of isolated human eccrine sweat glands.

This paper describes a method for isolating and studying the metabolism of human eccrine sweat glands. (a) Electron microscopy of glands which had been isolated and then incubated for an hour revealed no apparent alteration in morphology. (b) Known variation in gland size (male > female > children) was reflected in the relative rates of lactate production. (c) Lactate production was approximately 1.5 nmoles/gland per hr in the absence of glucose and rose to 2.7 at physiological concentrations of glucose (5.6 mmoles/liter). This amount of lactate production agrees well with the amounts found in sweat. (d) Both adrenergic (epinephrine) and cholinergic (methacholine) stimuli increased lactate production. (e) Glycogen depletion was demonstrated during incubation. (f) O(2) consumption was measured and aerobic metabolism was found to account for less than 1% of the energy derived from anaerobic pathways. These studies demonstrate that the large amounts of lactate appearing in human eccrine sweat can be accounted for by glandular metabolism and that both glycogen and glucose can be used as substrates.

Age Factors↗

PubMed1995

Analysis of sweat chloride by reversed phase high-performance liquid chromatography.

A high-performance liquid chromatography (HPLC) method for the determination of sweat chloride was developed and evaluated. This method is capable of measuring chloride ion in sweat eluates with an analytical working range from 0.5 mmol/L to 6 mmol/L (15 to 180 mmol/L in undiluted sweat samples). Precision studies showed that at the levels of 1 mmol/L, 2.5 mmol/L and 3.5 mmol/L, the within batch coefficients of variation were 1.26%, 0.88% and 0.43%, respectively, and the between batch coefficients of variation were 1.31%, 1.75% and 1.07%, respectively. The minimum detection limit was 0.5 mmol/L. This method correlated well with the ion-selective electrode (ISE) method currently in use.

Chlorides↗

PubMed2000

Applicability of different antibodies for immunohistochemical localization of CFTR in sweat glands from healthy controls and from patients with cystic fibrosis.

The hereditary disease cystic fibrosis (CF) is caused by mutations in the cystic fibrosis transmembrane conductance regulator (CFTR) gene. Understanding of the consequences of CFTR gene mutations is derived chiefly from in vitro studies on heterologous cell cultures and on cells hyperexpressing CFTR. Data from ex vivo studies on human tissue are scarce and contradictory, a fact which is in part explained by secondary tissue destruction in most affected organs. The purpose of this study was to establish conditions under which wild-type and mutated CFTR can be studied in affected human tissue. Sweat glands carry the basic defect underlying CF and are not affected by tissue destruction and inflammation. Therefore, we used this tissue to test a panel of eight different CFTR antibodies under various fixation techniques. The antibodies were tested on skin biopsy sections from healthy controls, from CF patients homozygous for the most common mutation, DeltaF508, and from patients carrying two nonsense mutations. Of the eight CFTR antibodies, only three-M3A7, MATG 1104, and cc24-met the criteria necessary for immunolocalization of CFTR in sweat glands. The labeling pattern in the CF sweat glands was consistent with the postulated processing defect of DeltaF508 CFTR. The antibodies exhibited different sensitivities for detecting DeltaF508 CFTR.

Acetone↗

PubMed1988

Ultrastructural localization of ouabain-sensitive, K-dependent p-nitrophenyl phosphatase activity in monkey eccrine sweat gland.

We studied the electron microscopic localization of ouabain-sensitive, potassium-dependent p-nitrophenyl phosphatase (K-pNPPase) activity of the Na K-ATPase complex in Rhesus monkey eccrine sweat gland by use of the one-step lead citrate method of Mayahara et al. (Histochemistry 1980; 67:125). Reaction product was observed predominantly in the cytoplasmic side of the basolateral membranes of clear (secretory) cells, especially in the interdigitating membrane folds in the basal labryinth, and were completely abolished by 10 mM ouabain or by removal of K+. Little or no enzyme activity was noted in membrane processes in the intercellular canaliculi and in the secretory coil lumen. Basolateral membranes of the dark cells also showed moderate enzyme activity. The myoepithelial cell membrane was devoid of reaction product, except in a few membrane processes arising from the inner aspect of myoepithelial cells. In the coiled duct, K-pNPPase activity was present predominantly in the entire cell membrane of the peripheral ductal cells. The predominantly basolateral distribution of Na-K-ATPase in the eccrine sweat secretory cells is consistent with the concept that a Na-K-Cl co-transport model may be involved in the mechanism of eccrine sweat secretion.

4-Nitrophenylphosphatase↗

PubMed1991

beta-adrenergic sweat responses in cystic fibrosis heterozygotes with and without the delta F508 allele.

Cystic fibrosis (CF) causes early death for most homozygotes, yet has a carrier frequency among Caucasians of about 4-5%, suggesting a heterozygote advantage. The major defect in the CF gene is a three-base deletion leading to loss of a phenylalanine residue at position 508 (delta F508) that accounts for about 68% of CF alleles in the North American population; the remaining 32% appears to consist of a large assortment of mutations. Sweat secretion in response to beta-adrenergic stimulation is completely lacking in CF homozygotes and is reduced to 1/2 normal in heterozygotes. To determine if this secretory process is affected by different CF alleles, we used the polymerase chain reaction technique with DNA obtained from peripheral leukocytes to determine retrospectively the presence or absence of the delta F508 allele in 20 CF heterozygotes for whom sweat responses to beta-adrenergic stimulation had previously been determined. Twelve of 20 subjects (60%) were positive for the delta F508 mutation. The variance in sweat responses was not reduced in the delta F508 group relative to the non-delta F508 group, but a gender/allele interaction was noted.

Alleles↗

PubMed2002

Improvement of diabetic autonomic gustatory sweating by botulinum toxin type A.

Fourteen diabetic subjects with gustatory sweating were treated by intracutaneous injections of botulinum toxin type A into the affected facial skin areas. In all subjects, sweating (measured by Minor starch iodine test) ceased within 4 days, with the maximal follow-up time lasting 24 weeks. This therapeutic approach, which could be used to reduce the severity of diabetic gustatory sweating, appears to be long lasting, adverse effect free, and minimally invasive.

Aged↗

PubMed1994

Extracellular ATP can activate autonomic signal transduction pathways in cultured equine sweat gland epithelial cells.

Changes in intracellular free calcium concentration ([Ca2+]i) were monitored in a cell line that was derived from the equine sweat gland epithelium. ATP and closely related compounds could increase [Ca2+]i with a rank order of potency of UTP > or = ATP > ADP >> AMP = adenosine = alpha,beta-methylene-ATP. The responses to ATP and to UTP were initiated by the release of calcium from an internal store and subsequently sustained by calcium influx. The rise in [Ca2+]i thus seems to be mediated by P2U receptors that are coupled to phosphoinositidase C. Some desensitisation of this response developed during repeated stimulation with ATP and this was blocked by staurosporine, an inhibitor of protein kinase C, and augmented by a phorbol ester which acts as an exogenous activator of this enzyme. A protein-kinase-C-dependent inhibitory pathway thus seems to become active during repeated stimulation with ATP. ATP and related compounds could also raise cellular cyclic AMP content. The order of potency was ATP > ADP = AMP = adenosine >> UTP, suggesting that this response is mediated via a separate subclass of P2 receptor. The present results demonstrate that ATP can activate autonomic signal-transduction pathways in cultured equine sweat gland cells and suggest that there may be a purinergic component to the control of secretory activity in the equine sweat gland.

Adenosine Triphosphate↗

PubMed1998

Regulation of ion content in primary cultures from reabsorptive ducts of human sweat glands studied by X-ray microanalysis.

X-ray microanalysis was used to investigate whether cAMP- and/or Ca2+-activated regulation of chloride and potassium efflux is expressed in primary cultures of sweat gland duct cells. The effects of extracellular UTP and ATP on the duct cells, and the signalling system involved in the response to ATP was also studied. Primary cultures from duct cells of human sweat glands responded to 1 microM carbachol, 2 microM of the Ca2+ ionophore A23187, or 5 mM 8-bromo-cAMP stimulation for 5 min, resulting in a decrease in cellular Cl and K concentrations. 50 microM 5-nitro-2-(3-phenylpropyl-amino)-benzoic acid (NPPB), a Cl- channel blocker, can inhibit the decrease in Cl concentration induced by cAMP. Extracellular (200 microM) ATP caused a decrease of Cl and K in cultured duct cells, while (200 microM and 2 mM) UTP was ineffective. Both the phosphoinositidase C inhibitor U73122 (10 microM) and the absence of extracellular Ca2+ abolished the ATP-induced decrease in Cl and K content. Alloxan (1.25 mM), an adenylate cyclase inhibitor, had an inhibitory effect on the response to ATP. The decrease in K, but not in Cl, content in the cells elicited by ATP was blocked by prior incubation with 100 ng/ml pertussis toxin, indicating the coupling of ATP to pertussis toxin-sensitive G-proteins. In conclusion, both Ca2+- and cAMP-dependent Cl- permeability is present in primary cultures from duct cells of human sweat gland. The response to ATP can be mediated both by Ca2+- and by cAMP-dependent pathways, and is coupled to pertussis toxin-sensitive G-proteins.

Adenosine Triphosphate↗

PubMed1978

Metastatic sweat gland carcinoma.

In summary the diagnosis of malignant non-metastasizing sweat gland carcinoma is difficult. In cases of metastasizing malignant sweat gland carcinomas, one must distinguish this lesion from metastastic breast carcinomas. The regional lymph nodes are the most likely site of metastasis in sweat gland carcinomas. Wide excision of the primary tumor with resection of the involved regional lymph nodes is considered to be the best initial treatment. Cure rates are in general poor, based on the small number of cases and inadequate follow-up.

Adenocarcinoma↗

PubMed2006

Luteal phase of the menstrual cycle increases sweating rate during exercise.

The present study evaluated whether the luteal phase elevation of body temperature would be offset during exercise by increased sweating, when women are normally hydrated. Eleven women performed 60 min of cycling exercise at 60% of their maximal work load at 32 degrees C and 80% relative air humidity. Each subject participated in two identical experimental sessions: one during the follicular phase (between days 5 and 8) and the other during the luteal phase (between days 22 and 25). Women with serum progesterone >3 ng/mL, in the luteal phase were classified as group 1 (N = 4), whereas the others were classified as group 2 (N = 7). Post-exercise urine volume (213 +/- 80 vs 309 +/- 113 mL) and specific urine gravity (1.008 +/- 0.003 vs 1.006 +/- 0.002) changed (P < 0.05) during the luteal phase compared to the follicular phase in group 1. No menstrual cycle dependence was observed for these parameters in group 2. Sweat rate was higher (P < 0.05) in the luteal (3.10 +/- 0.81 g m-2 min-1) than in the follicular phase (2.80 +/- 0.64 g m(-2) min(-1)) only in group 1. During exercise, no differences related to menstrual cycle phases were seen in rectal temperature, heart rate, rate of perceived exertion, mean skin temperature, and pre- and post-exercise body weight. Women exercising in a warm and humid environment with water intake seem to be able to adapt to the luteal phase increase of basal body temperature through reduced urinary volume and increased sweating rate.

Adult↗

PubMed1995

Sweat responses to pesticide-proof clothing influenced by textile materials.

Themoregulatory responses were compared at an ambient temperature of 30 degrees C and a relative humidity of 50% between two kinds of protective clothing for pesticide spraying. One was made of nylon with wet coating polyurethane (A) and another was made of cotton with water repellent finish (B). The clothing ensemble was composed of a short-sleeved cotton shirt, long cotton underpants, cotton socks, shoes and the protective clothing. Five young female adults served as subjects. They took a rest with protective clothing ensemble for 15 min and then walked on a motor-driven treadmill (80m/min, 5% uphill grade) for 40 min, followed by 20-min rest. The increase of forearm sweat rate was higher in clothing A than in clothing B in 4 out of 5 subjects, although the average values were not significantly different. The total sweat rate of the whole body was also significantly larger in clothing A. Mean skin temperature and local skin temperatures in the arm, the chest and the thigh were significantly higher in clothing A than in clothing B. Clothing microclimate humidity was significantly higher in clothing A, while clothing microclimate temperature tended to be higher in clothing A. Individual observations about thermal, humidity and comfort sensation disclosed that the sensation was improved as a whole in clothing B. Local sweat rate was linearly related to mean body temperature and the regression line for clothing A was located above that for clothing B in 4 out of 5 subjects.(ABSTRACT TRUNCATED AT 250 WORDS)

Adult↗

PubMed1996

Effects of head cooling on sweat rate in exercising subjects wearing protective clothing and mask for pesticide.

This study examined the effects of the head cooling on the sweat rates in 6 healthy female subjects at an ambient temperature of 28 degrees C and a relative humidity of 60%. The exercising subjects wore protective clothing and a mask for pesticide. Head cooling was made by putting frozen gel strips on the parietal and the front head which were held by a net-bandage (with cooling). On separate days, the same experiments were made without head cooling (without cooling). The total weight of the frozen gel strips was 250 g. After the subjects rested for 15 min in a chair, they repeated three times the work/rest schedule of 15 min exercise on a bicycle ergometer (50 W) followed by 5 min rest. Frozen gel strips were put on the head just before the first exercise bout. Main results were summarized as follows: 1) The forearm sweat rate, heart rate and the changes in Tre from the initial level (delta Tre) were significantly lower "with cooling" than "without cooling". 2) The amount of sweat adhered to the clothing ensemble was significantly lower "with cooling" than "without cooling". 3) The changes in Tty from the initial level (delta Tty) tended to be clearly lower "with cooling" than "without cooling". 4) Skin temperatures of the forehead and the cheek under a mask tended to be lower "with cooling" than "without cooling". 5) Subjective sensation ratings seemed to be improved in most subjects "with cooling". Thus, it was concluded that head cooling seemed to be more effective in reducing thermal strain in working subjects wearing the protective clothing and a mask for pesticide at hot environments.

Adult↗

PubMed1984

Contribution of skin thermal sensitivities of large body areas to sweating response.

The thermal sensitivity of different parts of the body was investigated by heating large areas of the body surface while the mean skin temperature calculated from Hardy and DuBois ' formula (1938) was always kept constant. The right arm sweating responses recorded under a local thermal clamp were related to changes in segmental skin temperatures of the different parts of the body. The results show that: 1) the various local peripheral signals are projected into integrating structures in the central nervous system; 2) the thermal sensitivity is greater for the head-and-trunk area in comparison with other parts of the body. For resting nude subjects, the formula of Hardy and DuBois remains a pertinent way for evaluating the role of skin thermal signals in the central drive for sweating. The peripheral contribution to the central sweating drive depends only on the skin temperature change and on the size of the stimulated area.

Adult↗