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At least 1,099 records · Page 61Linked to original sources
PubMed2002

Broad-host-range mobilizable suicide vectors for promoter trapping in gram-negative bacteria.

Here we report the construction of three different vectors for the identification of bacterial genes induced in vitro and/or in vivo. These plasmids contain kanamycin, gentamicin, or tetracycline resistance genes as selectable markers. A promoterless cat and an improved GFP (mut3-gfp) can be used to follow the induction of gene expression by measuring chloramphenicol resistance and fluorescence, respectively.

Chloramphenicol O-Acetyltransferase↗
PubMed1999

[Construction of a GFP broad-host-range promoter-probe vector and its application].

The 0.9 kb DNA fragment was obtained by PCR and using a pair of special designed primers from the green fluorescent protein gene(gfp). It Was then cloned into an expression vector pET-11C and named as pHN115. A promoferless gfp vector with SD fragment was obtained from pHN115 and subcloned into SK(+), pIJ 2925 and finally into pTR102. A broad-hast-range and visible promoter-probe vector, pHN127 was constructed and used successfully for the identification of constitutive and inducible promoters from Sinorhizobium fredii HN01.

Cloning, Molecular↗
PubMed2005

A complex of three new white-spored, sympatric, and host range limited Geosmithia species.

All hypocrealean species of the genus Geosmithia are anamorphic fungi with connections to bark beetles. G. fassatiae, G. langdonii and G. obscura are described as new sympatric species associated with Scolytus carpini, S. intricatus and S. rugulosus in Central Europe. The species represent a complex of three sister taxa with affinities to G. flava that may be distinguished by differences in morphology, unique RAPD patterns and by sequences of ITS region rDNA. Intraspecific variability and habitat specificity of new species is described and discussed. The high morphological, genetic and ecological uniformity suggest that these Geosmithia spp. are recently derived. A key to all accepted hypocrealean species of the genus is provided.

Animals↗
PubMed2005

Determination of the races of potato virus X and its host rang in Karaj, Damavand and Ardabil, Iran.

Potato virus x (PVX) is found commonly in potato-growing areas, worldwide. It is an economically important virus which causes losses in tuber yield of approximate 5 to 15 percent. In a 2 year survey, potato leaf and tuber samples were collected from various fields in Damavand and Karaj. The initial isolations from potato were made by mechanical inoculation first to Gomphrena globosa L. and later to Dartura stramonium L. It was not transmitted by 2 species of Cuscuta but transmitted mechamically. The isolates were inoculated to Nicotiana glutinosa L. in which they were maintained throughout the work. Sap from infected N. glutinosa was ineffective after dilution to 10-6, after 10 minutes at 70 degrees C and after 10 weeks at room temperature. The virus had filamentous and slightly flexuous particles with a normal length of about 490-500 nm and 12 nm width. According to the symptoms, TIP results and serological comparisons, the compared isolates showed no difference and they belong to XN group. In order to estimate disease incidence, 773 tubers from Damavand area were tested and compared with that in Ardabil area. Disease incidence in Damavand ranged from 1.1-20.9 percent and was lower than disease incidence in Ardabil. In 8 genera of collected weeds from fields of potato and tomato samples by using test plants and serological methods, they didn't show existence of the potato virus x.

Iran↗
PubMed1989

Host range and particle morphology of some bacteriophages affecting pathovars of Xanthomonas campestris.

Seven bacteriophages active against different pathovars of Xanthomonas campestris were isolated from naturally infected plant material. All showed polyhedral heads and could be separated into two morphological groups according to their tail structures. Phages active against X. campestris pv. cucurbitae (XCU-P1 and XCU-P3) and X. campestris pv. holcicola (XHOL-P1) were described for the first time. Ninety nine bacterial strains belonging to 5 genera (Xanthomonas, Pseudomonas, Agrobacterium, Clavibacter and Erwinia) were tested for sensitivity to the different phage suspensions. More than 60% of the Xanthomonas cultures were susceptible to one or more phages. The other genera did not react with any of the phages. The 7 phages were specific at a generic level but showed a low degree of specificity at the pathovar level.

Bacteriophages↗