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At least 1,135 records · Page 63Linked to original sources
PubMed1981

[Growth of mumps virus in human lung carcinoma cell lines. I. Host-range variant adapted under the presence of anti-mumps virus antibody (author's transl)].

The wild mumps virus which had been isolated from a patient suffering from mumps meningitis propagated biphasically in human lung cancer cell lines (Pc-3, Pc-10), while attenuated Urabe strain propagated monophasically. The early virus was detected in these cell lines when the cells were inoculated with the wild mumps virus at any multiplicity of infection but rapidly disappeared. The infectious virus, thereafter, could not be detected for about 20 days. However, the infectious virus appeared again and the titer of the virus in the culture fluid gradually increased. The late virus rapidly propagated in Pc-3 cells, while the early virus replicated in the same way as the original wild virus did. All of ten clones obtained from the original wild virus represented the biphasic growth in Pc-3 cells and the late virus was detected on the 25th-30th day after infection. The viral antigens in Pc-3 cells infected with wild virus did not disappear after cultivating under the presence of anti-mumps virus antibody for over 50 days. Furthermore, treatment of anti-mumps virus serum in the early period did not affect the appearance of the late virus. It is quite conceivable that the wild mumps virus might be able to persist in completely latent state and the adaptation of the virus to Pc-3 cells might progress during the latent period.

Adaptation, Biological↗
PubMed2002

The active partition gene incC of IncP plasmids is required for stable maintenance in a broad range of hosts.

Plasmids of incompatibility group P (IncP) are capable of replication and stable inheritance in a wide variety of gram-negative bacteria. Three determinants of IncP plasmids are components of an active partition locus that is predicted to function in the segregation of plasmid copies to daughter cells. These determinants are incC, which codes for a member of the ParA family of partition ATPases; korB, which specifies a DNA-binding protein that also functions as a global transcriptional repressor; and O(B), the DNA target for KorB, which occurs at multiple locations on IncP plasmids. To determine the importance and host range of the IncC/KorB partition system in the maintenance of IncP plasmids, we constructed an in-frame deletion of incC in the otherwise intact 60-kb IncP alpha plasmid R995. R995 Delta incC was found to be highly unstable in Escherichia coli, Pseudomonas aeruginosa, Pseudomonas putida, Agrobacterium tumefaciens, and Acinetobacter calcoaceticus, whereas wild-type R995 is stable in all these hosts. In addition, R995 Delta incC could not be established in Actinobacillus actinomycetemcomitans. trans-Complementation analysis showed that the coding region for IncC2 polypeptide, which is expressed from an internal translational start within the incC gene, was sufficient to restore stable maintenance to wild-type levels. The results show that the IncC/KorB active partition system of IncP plasmids is remarkably proficient for stable maintenance in diverse bacteria.

Bacterial Proteins↗
PubMed1992

[Analysis of the conjugation system of plasmid pBS1001 with a broad range of hosts].

Tn5-induced tra mutations were localized on the physical map of a broad host range plasmid pBS1001. Mutations were united into three clusters covering 25% of plasmid DNA. They were distributed in 7 groups by complementation analysis. It was shown that coexistence of tra mutants of pBS1001 and RP4 within the same cell did not restore conjugation properties of both plasmids. High frequency mobilization of some known vectors by pBS1001 was demonstrated.

Conjugation, Genetic↗
PubMed1986

[Structural-functional organization of R-plasmid pBS222 with a broad range of bacterial hosts].

The broad host-range plasmid pBS222 is compatible with broad host-range plasmids of all known incompatibility groups and codes for tetracycline resistance. pBS222 is efficiently mobilized by Inc P-1 plasmid RP4 and is also capable of conjugal transfer with low efficiency to different gramnegative microorganisms. The size of the plasmid (17.2 Kb) has been determined and its physical map has been constructed. The plasmid harbours the unique sites for restriction endonucleases BglII, HindIII, HpaI, KpnI, SmaI and XbaI cleawage. The plasmid derivatives pBS352-pBS355 have been obtained that carry kan- and cam-determinants in addition to tet-gene. Plasmid pBS355 has been used to clone EcoRI-fragments of phage lambda DNA. The plasmid pBS222 regions essential for replication and maintenance have been localized by DNA hybridization analysis of its mini-derivatives pBS356 and 357. pBS222 is a convenient model for investigations of the plasmid replication and maintenance mechanisms in different bacterial hosts as well as for the construction of broad host-range vectors.

Conjugation, Genetic↗
PubMed1989

[Isolation and characteristics of a recombinant pOV13 plasmid as a vector for DNA cloning in a broad range of bacterial hosts].

The hybrid plasmid pOV13 proposed as a potential vector for DNA cloning in a broad bacterial host range has been constructed on the basis of the broad host range plasmid RSF1010 and a shortened derivative of RP4, the plasmid pVZ115 serving a marker DNA fragment. The plasmid pOV13 contains the genes for streptomycin, kanamycin and tetracycline resistance and single cleavage sites for restriction endonucleases BamHI, BgIII, SalI, SmaI, PvuII, XhoI, as well as double cleavage sites for restriction endonucleases PstI and HindIII permitting one to clone DNA with insertional inactivation of genes. The physicogenetical map of the birepliconed plasmid pOV13 is presented.

Cloning, Molecular↗