Reproductive performance following conservative microsurgical management of tubal pregnancy.
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Genital prolapse is a disorder of pelvic support and is one of the most frequent disorders encountered in our gynaecological practice. Our social and cultural background predisposes to this condition to occur at an age which is reported to be earlier than any part of the world. Over a period of 4 years, 1986-1989, 17 cases were studied in whom the Sleeve Excision anastomosis operation was carried out, either at the Lokmanya Tilak Municipal General Hospital, Sion, Bombay, India or in some other hospital. All these patients were either admitted in active labour or as cases of abortions or were being treated for infertility. The incidence of full-term normal vaginal delivery in our study was 66.6% and the incidence of Caesarean section was 8.3%. There was 1 case of posterior wall rupture following previous sleeve excision anastomosis operation. The recurrence rate of prolapse in our series was only 7.7%. The Sleeve excision anastomosis operation has given excellent anatomical and obstetric results in our study and future multicentric trials will be necessary to study its effect on subsequent fertility to arrive at any final conclusion.
PROBLEM: Immunological factors may account for previously unexplained cases of recurrent abortion. METHOD: After screening 76 couples for causes of recurrent spontaneous abortion and measuring maternal antipaternal immunity, 23 primary spontaneous recurrent aborters were immunized once with their husbands' leukocytes. Testing for antipaternal cytotoxicity was repeated in 21 couples. Seroconversion was significantly less frequent in couples who shared more than one human leukocyte antigen [one of five (20%) versus 13 of 16 (81%), P < .02]. RESULTS: Twelve of 16 women (75%) who became pregnant had live children and five of those have had a second live child. All 12 women who achieved successful pregnancies had become antipaternal cytotoxic antibody-positive after immunization, whereas all four patients who had repeat abortions had failed to seroconvert (P < .001). However, this relationship is not necessarily causative, as the successful group also tended to have fewer previous abortions and less human lymphocyte antigen sharing. CONCLUSION: Except for transient illness after immunization, one moderately small for gestational age baby and one premature labor at 32 wk, no complications were observed after immunization.
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A total of 1061 lactating dairy cows in six different herds were randomly allocated to treatment and control groups. One herd was lot-fed on total mixed rations; three herds were fed on pasture with significant amounts of supplementary concentrates, and two herds were primarily pasture fed. Treated cows received a slow-release, intraruminal capsule containing 32 g sodium monensin within 7 days of calving. Conception rates at first service, days to first oestrus and calving-to-conception interval did not differ significantly between untreated and monensin-treated cows in the 5 herds, 3 herds and 4 herds, respectively, in which these outcomes were examined. Treatment of lactating cows immediately after calving may not be the optimal method to achieve fertility responses with capsules containing sodium monensin. Monensin treatment significantly increased milk yield in one of the six herds. Milk fat or milk protein production was not significantly affected by treatment.
A randomised trial was conducted to determine the effects of sodium monensin on plasma metabolite concentrations, resumption of cyclicity and oestrus and milk production and milk composition of postparturient dairy cows. Cows treated with sodium monensin in a controlled-release capsule had significantly lower plasma beta-hydroxybutyrate (BHB) concentrations (P = 0.006) and tended (P = 0.07) to have significantly higher plasma glucose concentrations than untreated cows. Treatment did not significantly influence plasma free fatty acid, urea nitrogen or cholesterol concentrations. Despite higher plasma glucose concentrations and lower plasma BHB concentrations, periods from calving to first ovulation and oestrus were not reduced by treatment. This observation, with limited numbers of cows, is not consistent with a hypothesis that glucose or ketones are important metabolic regulators of pulsatile release of luteinising hormone and consequent resumption of cyclicity. While milk production and milk protein concentration did not significantly differ between groups, milk fat concentration was significantly lower for treated cows. Treated cows probably had lower dry matter intake than untreated cows as they produced 67 litres less milk per head and lost more weight than untreated cows during the study period, although weight and body condition score were not significantly affected by treatment. The lack of significance of these latter observations probably reflects the low statistical power of the study.
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The incidence of periparturient diseases and their effects on the calving to first service interval, the calving to conception interval and the mean number of services per conception were recorded in five Friesian-Holstein herds in the United Kingdom. The incidence rates of milk fever, milk fever followed by endometritis, retained fetal membranes, endometritis after retained fetal membranes, endometritis, or ovarian cysts, were 7.5, 1.4, 1.3, 3.3, 10.1 and 6.7 per cent, respectively, and the affected cows had significantly (P less than 0.05) extended calving to conception intervals (13, 66, 25, 51, 31 and 64 days, respectively) compared with herd-mates. Calving to first service intervals and services per conception were also adversely affected. In spite of treatment, milk fever, endometritis and ovarian cysts had a considerable impact on fertility. Improved methods of treatment and, or, prevention are clearly required.
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Early postpartum (6 weeks) ovarian activity, hormonal profiles, uterine involution, uterine infections, serum electrolytes, glucose, milk acetoacetate and blood urea nitrogen (BUN) levels were studied in 2 Estonian high producing dairy herd with annual milk production of 7688 (Farm A) and 9425 (Farm B). From each farm 10 cows, with normal calving performance were used. Blood samples for the hormonal (PGF2alpha-metabolite, progesterone) analyses were withdrawn. On day 25 PP blood serum samples were taken for the evaluation of metabolic/electrolyte status. On the same day estimation of milk acetoacetate values was done. The ultrasound (US) was started on day 7 PP and was performed every 3rd day until the end of experiment. Uterine content, follicular activity and sizes of the largest follicle and corpus luteum were monitored and measured. Vaginal discharge and uterine tone were recorded during the rectal palpation. Each animal in the study was sampled for bacteriological examination using endometrial biopsies once a week. Two types of PGF2alpha-metabolite patterns were detected: elevated levels during 14 days PP, then decline to the basal level and then a second small elevation at the time of final elimination of the bacteria from the uterus: or elevated levels during first 7 days PP, then decline to the basal level and a second small elevation before the final elimination of bacteria. Endometritis was diagnosed in 5 cows in farm A and in 3 cows in farm B respectively. In farm A, 5 cows out of 10 ovulated during experimental period and in 1 cow cystic ovaries were found. In farm B, 3 cows out of 10 ovulated. In 3 cows cystic ovaries were found. Altogether 40% of cows had their first ovulation during the experimental period. Three cows in farm A and 5 cows in farm B were totally bacteria negative during the experimental period. The most frequent bacteria found were A. pyogenes, Streptococcus spp., E. coli., F. necrophorum and Bacteroides spp. The highest incidence of bacteriological species was found during the first 3 weeks in both farms. All animals were free from bacteria after 5th week PP in farm A and after 4th week in farm B respectively. Serum electrolytes and glucose levels were found to be within the reference limits for the cows in both farms. No significant difference was found between farms (p > 0.05). Low phosphorus levels were found in both farms. Significant difference (p < 0.05) was found in BUN levels between farms. In both farms milk acetoacetate values were staying within the reference range given for the used test (< 100 micromol/l). The uterine involution and bacterial elimination in the investigated cows could consider as normal but more profound metabolic studies could be needed to find reasons for later resumption of ovarian activity. Some recommendations to changing feeding regimes and strategies should also be given.
Egg mass shows large intraspecific variation in birds and yet the mechanisms underlying this variation remain unknown. We hypothesized that estradiol would play a central role in determining egg mass, since this hormone stimulates the production of yolk precursors (vitellogenin and very-low density lipoprotein, VLDL) by the liver, and of albumen by the oviduct. We gave European starlings (Sturnus vulgaris) silastic implants containing estradiol prior to egg formation, which we predicted would increase egg mass. As expected, exogenous estradiol stimulated a marked (49 %) increase in plasma vitellogenin levels at the beginning of laying. At clutch completion, plasma VLDL levels and oviduct mass were also elevated in estradiol-treated females compared with controls. However, estradiol had no effect on fresh egg mass or clutch size. Estradiol treatment actually decreased the mass of yolk protein and lipid, perhaps by decreasing the rate of uptake of yolk precursors at the ovary. The failure of estradiol to increase egg mass indicates that this phenotype may be regulated at higher levels of organization (e.g. negative feedback, uptake of yolk precursors) than those studied in this experiment. Despite elevating yolk precursor levels, treatment with estradiol had no effect on the mass of the liver or endogenous stores of protein and lipid at clutch completion.