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Optical influence of ship wakes.

The optical variations observed within ship wakes are largely due to the generation of copious amounts of air bubbles in the upper ocean, a fraction of which accumulate as foam at the surface, where they release scavenged surfactants. Field experiments were conducted to test previous theoretical predictions of the variations in optical properties that result from bubble injection in the surface ocean. Variations in remote-sensing reflectance and size distribution of bubbles within the ship-wake zone were determined in three different optical water types: the clear equatorial Pacific Ocean, moderately turbid coastal waters, and very turbid coastal waters, the latter two of which were offshore of New Jersey. Bubbles introduced by moving vessels increased the backscattering in all cases, which in turn enhanced the reflectance over the entire visible and infrared wave bands. The elevated reflectance had different spectral characteristics in the three locations. The color of ship wakes appears greener in the open ocean, whereas little change in color was observed in near-coastal turbid waters, consistent with predictions. Colorless themselves, bubbles increase the reflected radiance and change the color of the ocean in a way that depends on the spectral backscattering and absorption of the undisturbed background waters. For remote observation from aircraft or satellite, the foam and added surfactants further enhance the reflectance to a degree dependent on the illumination and the viewing geometry.

Journal Article↗

Variation of the pH of the background electrolyte due to electrode reactions in capillary electrophoresis: theoretical approach and in situ measurement.

Electrode reactions during the electrophoretic process may change the pH of the buffer and subsequently the migration behavior of solutes with resultant loss of reproducibility. A theoretical treatment of pH variations due to electrolytic processes is presented. The choice of buffer appears to have a dramatic influence on the pH variations observed, even if substantial buffer action is expected at the pH chosen. The experimental evaluation of the separation of 4-hydroxy-3-methoxycinnamic acid and 3-hydroxybenzoic acid reveals that the quality of the separation decreases continuously from a baseline separation observed in the first experiment to a comigration of the two solutes (resolution = 0) in the ninth experiment. A pH decrease of about 0.05 pH units accounts for the observed changes in mobility. A novel in situ pH measurement approach is presented, in which the mobility, peak area, and peak height of an indicator dye are related to the pH in the capillary. This enables the identification and quantitation of pH variations during electrophoretic runs: the pH decreases at the anodic side already after the first experiment and pH variations as small as 0.02 pH units can be measured. The variations in peak height appear to be less suited. The calculated pH variations are in close agreement with the ones obtained experimentally.

Buffers↗

Multilevel correlates of childhood physical aggression and prosocial behavior.

The study identified independent individual, family, and neighborhood correlates of children's physical aggression and prosocial behavior. Participants were 2,745 2-11-year olds nested in 1,982 families, which were themselves nested in 96 Canadian neighborhoods. Hierarchical linear modeling showed that the total variation explained by the three-level model was 28.03% for physical aggression and 17.57% for prosocial behavior. For both childhood behaviors, approximately 66% of this explained variance was between individuals and up to 30% was between families. The smallest amount of observed variation was between neighborhoods. Significant individual-level predictors common to both childhood behaviors were child's sex and maternal hostility toward the target child. Specifically, boys had more mother-reported physical aggression and less prosocial behavior. Children who experienced greater-than-average maternal hostility (compared to siblings) were more physically aggressive and less prosocial. At the family level, significant common predictors were mother depressed mood and punitive parenting. Children had higher levels of physical aggression and lower levels of prosocial behavior in families where mothers had greater depressed mood and used more punitive parenting practices. At the neighborhood level, greater perceived problems and lower poverty level were associated with higher levels of physical aggression. Results are discussed with reference to past and future studies of multilevel effects on children's socialization.

Aggression↗

In vitro studies of microwave-induced cataract: reciprocity between exposure duration and dose rate for pulsed microwaves.

Rat ocular lenses exposed to pulsed microwave irradiation were maintained at constant temperature by circulating phosphate buffered saline in a thermostatically-controlled chamber. Irradiations with pulsed radiation (10 musec, 24 kW pulses) of 918 MHz were done at several different specific absorption rates (SAR) for durations up to 1 hr in order to explore a possible reciprocal relationship. The extent of damage was measured by the maximum depth of granular degeneration in the equatorial region of lenses fixed immediately after irradiation. The parameters of the pulses were increased to 20 musec and 48 kW to explore the variation in the biological effects and threshold with respect to average power, as well as pulse parameters (pulse width, peak power and energy per pulse). A total of 47 lenses were used in 3 X 4 factorial experimental design to explore effects observed at different average powers and durations (6, 20 and 60 min). The results were statistically analyzed by ANOVA and multiple regression analysis with logarithmic transformation. The results are summarized as follows. This data showed clear trends towards increasing depth of granular degeneration with increasing duration of exposure and dose rate. There was considerable evidence to confirm such reciprocity suggesting that total dose is an important parameter. A model postulating reciprocity was shown to explain observed variation in depth of damage as well as one allowing for separate effects of duration and dose rate. Lens fibre cell effects were detected by scanning electron microscopy after 6 min irradiation at the SAR values of 40 and 20 mW g-1. Light microscopic evidence of lens fiber cell damage can be detected at an SAR of 10 mW g-1 after a 1 hr exposure.

Animals↗

Variations in intergenic region sequences of Human respiratory syncytial virus clinical isolates: analysis of effects on transcriptional regulation.

Sequences at the beginnings and ends of Human respiratory syncytial virus (HRSV) genes are necessary for efficient initiation and termination of transcription. The gene start sequences are well conserved and contain signals required for initiation, while the semi-conserved sequences at the gene ends direct transcriptional termination with varying efficiencies. The intergenic regions, which lie between the gene ends and the downstream gene start sequences, are not conserved in length or sequence, and certain positions have been reported to play a role in transcriptional regulation. We have previously shown that the gene end sequences in HRSV subgroup A clinical isolates are variable and that variations found at certain gene ends decreased transcriptional termination and downstream mRNA expression. Here, we have extended this work to examine variation in the intergenic regions between the genes of clinical isolates. We determined the sequences of the eight intergenic regions and the M2/L overlap from clinical isolates from the US and UK and found that all of these regions contained variations from the prototype A2 strain. The amount of variation observed was disparate among the different intergenic regions and did not correlate with length. The effects of selected variant sequences on transcription were examined in the context of subgenomic replicons. While some changes in the intergenic regions had minor effects, certain sequence variations significantly altered transcription termination or initiation. A single nucleotide deletion in the M/SH intergenic region decreased initiation at the SH gene start seven-fold, while changes in the F/M2 intergenic region were found that in some cases increased and in others decreased termination at the F gene end. The P/M intergenic region was the most variable, but none of the changes examined affected either termination at the P gene end or initiation of the downstream M gene start. These results show that in HRSV clinical isolates the intergenic region sequences are variable and that changes in these regions have the potential to affect transcriptional control at the gene junctions.

Base Sequence↗

Variation in rates of diagnosis of acute otitis media.

Over 13 weeks during two periods in 1978 the diagnostic rate for acute otitis media was monitored among febrile children (aged one to five years) in the emergency room of a large children's hospital. Of the total 228 eligible children, 53 (23 percent) were diagnosed as having otitis media. The observed variation in the weekly diagnostic rates was significantly (p < .005) greater than that expected if the diagnosis rate were a constant 23 percent. Temporal variation in diagnostic rates was largely attributable to differences among individual providers and independent of level of training. For junior residents short-term learning, within the seven-week emergency room rotation, contributed significantly to the week-to-week temporal variation in diagnostic rates for acute otitis media.

Acute Disease↗

An epidemiological study of Salmonella enteritidis by pulsed-field gel electrophoresis (PFGE): several PFGE patterns observed in isolates from a food poisoning outbreak.

An epidemiological analysis of Salmonella enteritidis from a food poisoning was done using pulsed-field gel electrophoresis (PFGE) of BlnI- or XbaI-digested fragments of chromosomal DNA of isolates. S. enteritidis isolates obtained from 19 patients had identical PFGE patterns. Therefore, a strain giving the same pattern was considered to be the causative agent of this outbreak. In addition, four isolates that had different BlnI-digested PFGE patterns were obtained from three patients, suggesting that the observed variations in PFGE patterns might occur as the result of some point mutations of chromosomal DNA during growth or from the existence of several S. enteritidis strains from various sources. Subsequent PFGE analysis of continuously subcultured strains supported the former possibility. These observations indicate that PFGE analysis on multiple numbers of colonies from each patient are necessary for the epidemiologic investigation of S. enteritidis.

DNA, Bacterial↗

Constitutive heterochromatin DNA polymorphisms in diploid Medicago sativa ssp. falcata.

A Giemsa C-banding technique was used to study the amount and location of constitutive heterochromatin in diploid (2n = 2x = 16) Medicago sativa ssp. falcata (L.) Arcangeli. Most accessions had the standard C-banding pattern with centromeric bands on all the chromosomes and a prominent heterochromatic band at the nucleolar organizer regions (NOR) of the satellited (SAT) chromosomes. However, we observed in various accessions that constitutive heterochromatic C-bands can exist at the telomeric ends of all the chromosomes. Interstitial bands occurred on the short arms of all chromosomes except for chromosome 3 and on the long arms of chromosomes 1, 2, 3, and 6, only. Rearranged chromosomes such as isochromosomes have been observed for the short arms of chromosomes 2 and 6. This is the first report on the existence of C-banding polymorphisms and the detection of putative isochromsomes in the chromosomes of diploid ssp. falcata which could have contributed to the variation observed in cultivated alfalfa.

Chromosome Banding↗

Modelling and control of potable water chlorination.

The Umgeni Water Wiggins water treatment plant feeds the southern areas of Durban in South Africa and has a maximum treatment capacity of about 350 Ml/d. Two interconnected reservoirs at this facility hold treated water before it enters the distribution network. Because of the variable demand, the reservoir levels and residence times undergo considerable variation. This has a strong influence on the free chlorine concentration in the water leaving the reservoir, which should be 0.8 to 1.2 mg/l, to ensure an adequate disinfection potential within the network. This paper describes a model which accounts for the observed variations of chlorine concentration, and will form the basis of a predictive controller for the chlorine concentration in the outlet.

Chlorine Compounds↗

Hematocrits of free-ranging baboons: variation within and among populations.

Hematocrits among free-ranging baboons (Papio hamadryas subsp.), from Awash in Ethiopia and Mikumi in Tanzania, varied by region, sex, age, and season of collection. Tanzanian animals had higher mean values than Ethiopian, and hematocrits were higher in the dry season. We discuss the comparability of field and laboratory data and possible reasons for the observed variation.

Age Factors↗

Seasonal variation in serum cholesterol levels: treatment implications and possible mechanisms.

BACKGROUND: A variety of studies have noted seasonal variation in blood lipid levels. Although the mechanism for this phenomenon is not clear, such variation could result in larger numbers of people being diagnosed as having hypercholesterolemia during the winter. METHODS: We conducted a longitudinal study of seasonal variation in lipid levels in 517 healthy volunteers from a health maintenance organization serving central Massachusetts. Data collected during a 12-month period for each individual included baseline demographics and quarterly anthropometric, blood lipid, dietary, physical activity, light exposure, and behavioral information. Data were analyzed using sinusoidal regression modeling techniques. RESULTS: The average total cholesterol level was 222 mg/dL (5.75 mmol/L) in men and 213 mg/dL (5.52 mmol/L) in women. Amplitude of seasonal variation was 3.9 mg/dL (0.10 mmol/L) in men, with a peak in December, and 5.4 mg/dL (0.14 mmol/L) in women, with a peak in January. Seasonal amplitude was greater in hypercholesterolemic participants. Seasonal changes in plasma volume explained a substantial proportion of the observed variation. Overall, 22% more participants had total cholesterol levels of 240 mg/dL or greater (> or =6.22 mmol/L) in the winter than in the summer. CONCLUSIONS: This study confirms seasonal variation in blood lipid levels and suggests greater amplitude in seasonal variability in women and hypercholesterolemic individuals, with changes in plasma volume accounting for much of the variation. A relative plasma hypervolemia during the summer seems to be linked to increases in temperature and/or physical activity. These findings have implications for lipid screening guidelines. Further research is needed to better understand the effects of a relative winter hemoconcentration.

Adult↗

Central and peripheral catecholamines and phenylethanolamine-N-methyl-transferase activity during the oestrus cycle.

Natural variations in monoamine levels in the adrenal gland, whole brain, hypothalamus and plasma during the oestrus cycle were determined in Sherman rats. Noradrenaline content of adrenal gland was at its highest level during met-oestrus phase while adrenaline was at its lowest level. During pro-oestrus adrenaline in the adrenal gland was at the maximum value. Plasma adrenaline did not change significantly during the oestrus cycle. However plasma noradrenaline was significantly higher during metoesrus compared to the value of di-oestrus and pro-oestrus. In the hypothalamus noradrenaline level was at its highest value during di-oestrus and at its lowest during met-oestrus, while brian noradrenaline reached its maximum value during pro-oestrus. The sum of plasma adrenaline and noradrenaline showed maximum level during met-oestrus phase but at the same time total adrenal adrenaline plus noradrenaline was lowered. The variations in adrenaline and noradrenaline in the adrenal gland did not correspond to the changes in the activity of enzyme, phenylethanolamine-N-methyltransferase (PNMT). The activity of PNMT reached its maximum value during met-oestrus phase of the oestrus cycle. The observed variations in adrenal, brain, hypothalamus and plasma catecholamines can be attributed to the modification in endocrine activity which takes place during the oestrus cycle.

Adrenal Glands↗

Structural requirements for the inhibition of human monocyte carboxylesterase by organophosphorus compounds.

Human blood monocyte carboxylesterase (CBE) is inhibited by a variety of organophosphorus compounds including arylphosphates and arylphosphites and some alkylphosphites. Triphenyl phosphate and triphenyl phosphite with Ki values of 8 x 10(-9) M and 4.8 x 10(-8) M, respectively, are the most potent inhibitors of this enzyme evaluated by this study. The arylphosphates vary in their capacity to inhibit carboxylesterase activity. Diphenyl phosphate with its strong negative charge is not a potent inhibitor (Ki = 1 x 10(-4) M), whereas if its negative charge is neutralized, as in diphenyl methyl phosphate, its capacity to inhibit carboxylesterase is significantly increased. Compounds with increased bulk, such as trinaphthyl phosphate, only inhibit the enzyme at concentrations of 10(-5) M or greater. Arylphosphites have inhibitory capacities similar to the arylphosphates. Alkylphosphites (tributyl phosphite/triethyl phosphite) inhibit carboxylesterase activity, whereas alkylphosphates (tributyl phosphate/triethyl phosphate) have no inhibitory effect. Arylphosphines and arylphosphine oxides do not inhibit carboxylesterase activity. This study demonstrates that organophosphates and organophosphites are relatively effective inhibitors of human monocyte CBE activity with the exception of the alkylphosphates which have no inhibitory activity. We conclude that molecular bulk and charge have a significant role in determining the potency of organophosphorus inhibitors of monocyte CBE. The observed variations in the degree of esterase inhibition by organophosphorus compounds as well as the differences in the pathological expression of neuropathic disorders associated with such chemicals suggest that different esterase enzymes derived from the family of esterase genes may mediate the different neuropathies observed with organophosphorus exposures. Such data also provide the rationale for the kinetic analyses of esterases and the design of non-toxic organophosphorus compounds with low or no monocyte CBE inhibitory capacity to reduce the potential of these commonly used chemicals for human toxicity.

Binding Sites↗

Interpreting the von Bertalanffy model of somatic growth in fishes: the cost of reproduction.

We develop a model for somatic growth in fishes that explicitly allows for the energy demand imposed by reproduction. We show that the von Bertalanffy (VB) equation provides a good description of somatic growth after maturity, but not before. We show that the parameters of the VB equation are simple functions of age at maturity and reproductive investment. We use this model to show how the energy demands for both growth and reproduction trade off to determine optimal life-history traits. Assuming that both age at maturity and reproductive investment adapt to variations in adult mortality to maximize lifetime offspring production, our model predicts that: (i) the optimal age of maturity is inversely related to adult mortality rate; (ii) the optimal reproductive effort is approximately equal to adult mortality rate. These predictions are consistent with observed variations in the life-history traits of a large sample of iteroparous freshwater fishes.

Age Factors↗

Isolation of a distally located gene possibly correlated with gametocyte production ability.

Previous studies were focussed on the attempt to correlate observable variations in the size of Plasmodium berghei chromosomes with the loss of ability to produce viable gametocytes. A temporal coincidence between the appearance of a subtelomeric deletion on P. berghei chromosome 5 and the loss of the ability to produce viable gametocytes was observed in a clone (HPE) directly derived from the high gametocyte-producer clone 8417 during mechanical passages. Interestingly enough, three P. berghei sexual-specific genes have already been mapped on internal fragments of this chromosome. A novel gene, clone 150, isolated from a genomic library of clone 8417 using a probe enriched for sexual-specific transcripts, maps on chromosome 5 within 100kb from the telomere. Subtelomeric deletions of chromosome 5 affecting two non-producer clones involve part of the transcribed region of this gene.

Animals↗

[Regional differences in the use of cholecystectomy in Denmark during the period 1977-1985].

In a register-based study of all cholecystectomized patients in Denmark during the period from 1977-85 (37,048 patients) an account is given of the regional variations observed in connection with the intervention. The cholecystectomy operation is sub-divided into four types of operation dependent on whether the intervention is merely a matter of cholecystectomy or it involves the choledocus, the duodenum, the small intestine or other organs. The variation analysis is based on a division of the country into 75 areas, each primarily served by a single hospital. The method highly reflects the differences in clinical treatment strategies. The summarized rate of cholecystectomy for this period of time was 8.2 per 10,000 persons. In areas with the highest and the lowest rates of operation the figures were 12.3 per 10,000 and 5.1 per 10,000, respectively. In 18 areas, the rates of operation were significantly higher than average and in 18 other areas significantly higher than average and in 18 other areas significantly below average. SCV-score (X100) 2.19. There is no systematic correlation between the degree of specialisation in the operating department and the frequency in employing the operation. Areas with an operation index significantly above average had the same rate of more complicated operations than simple cholecystectomy as the other areas. Possible causes of national as well as international variations in the use of cholecystectomy are discussed, and the need for a radical evaluation of this kind of treatment is emphasized.

Adult↗

Variations in plasma protein concentrations in individuals with ulcerative colitis: analytical and biological factors.

Eight plasma proteins were determined in specimens taken every second day during a 14 day period from eleven patients with acute ulcerative colitis. The intra-individual variations in the concentrations of albumin, orosomucoid, haptoglobin, IgG, IgA, IgM and complement factors C3 and C4 were larger than expected in normal persons. A two-way analysis of variance was applied to the normalized protein values to estimate to what extent the observed variations could be explained by analytical errors and the influence of biological factors general to all proteins, such as changes in plasma volume and distribution between plasma and extravascular space. In half the non-operated patients the changes in all proteins could be explained by the above mentioned variations. The individual variations seen in the concentrations of haptoglobin, C4 and IgM occurred at random compared to the clinical state of the disease. Only the operated patients showed a more systematic sequence of protein changes.

Analysis of Variance↗

Lack of relationship of red cell enzyme activity to bilirubin and carboxyhemoglobin levels in healthy term infants.

A total of 32 term infants were studied in an attempt to confirm and extend the recent observation of Petrich & associates (14) that minor degrees of transient deficiences of the red cell enzymes glucose phosphate isomerase and glyceraldehyde-3-phosphate dehydrogenase were related to hyperbilirubinemia in otherwise healthy term infants. No relationships could be observed between the activity of these enzymes and the bilirubin level on day three. In addition, no correlation was present between bilirubin values and carboxyhemoglobin levels on day three, suggesting that in the healthy term infant excessive hemolysis is not usually responsible for the variations observed in bilirubin levels.

Bilirubin↗