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Results for “Spatial orientation”

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PubMed1992

Competitive and non-competitive NMDA receptor antagonists in spatial learning tasks.

The effects of the competitive N-methyl-D-aspartate (NMDA) receptor antagonists, CGP37849 (3 or 6 mg/kg i.p.) and its ethyl ester CGP39551 (5 or 15 mg/kg i.p.) and of the non-competitive NMDA receptor antagonist, dizocilpine (0.16 mg/kg; i.p.) on acquisition by rats of different spatial orientation tasks in an 8-arm radial maze were evaluated. Neither of the CGP compounds influenced locomotor activity during spontaneous alternation, only dizocilpine increased the number of arm entries (locomotion). Preferred angles between consecutive arm entries were changed by the high doses of CGP37849 (6 mg/kg), CGP39551 (15 mg/kg) and dizocilpine (0.16 mg/kg). The high doses of both CGP compounds as well as dizocilpine produced impairments in the acquisition of an egocentric orientation task and an allocentric reversal task indicated by an increased number of arm entries and re-entries. Such amnesic effects did not occur after administration of low doses of CGP37849 (3 mg/kg) and CGP39551 (5 mg/kg), doses which are sufficient to produce anticonvulsant and anticataleptic effects. In contrast, the non-competitive NMDA receptor antagonist, dizocilpine, produced amnesic effects over the entire behaviourally effective dose range.

2-Amino-5-phosphonovalerate↗

PubMed1993

Relationship between receptor affinity and topography of N-terminally extended and bridged [Tyr1-->Asp4]deltorphin C analogues: novel probes for the delta-opioid receptor.

Receptor binding of N-terminally extended Tyr1 deltorphin C analogues diminished delta and mu affinities, but with only a moderate loss in delta selectivity. Pseudopeptide bridged [Tyr1-->Aps4]deltorphin C analogues drastically decreased delta affinity to yield peptides with poor delta selectivity. Low energy conformers of the peptides revealed that the bridge modifies the spatial orientation of the backbone of the N- and C-terminal sequences with respect to deltorphin C. The data indicate that the delta receptor site can accommodate an opioid peptide containing an N-terminal aliphatic extension on amino-Tyr1, but not a heptapeptide conformationally constrained between residues 1 and 4.

Amino Acid Sequence↗

PubMed1988

A model for the delta-receptor-bound conformation of enkephalin.

Sets of low-energy structures were determined by energy calculations for two cyclic analogues of enkephalin (Ek), [D-Pen2, D-Pen5]-Ek and [D-Pen2, L-Pen5]-Ek, possessing the highest specificity towards delta-opioid receptors. Comparison of mutual spatial orientations of the alpha-amino group and aromatic moieties of the Tyr and Phe residues permitted one to suggest a model for the delta-receptor-bound conformation of enkephalin-related peptides. The model involves a pronounced gamma-like turn of the peptide backbone centred on the Gly3 residue.

Calorimetry↗

PubMed1983

Field measurements in snow skiing injury research.

Field measurements during skiing experiments simultaneously recorded the complete excitation of the toe and heel of one foot, the absolute spatial orientation of the pelvis and the foot, muscle activity at the hip, knee and ankle and the complete rotation of the femur relative to the tibia across the knee in three test subjects. Data were transmitted from test subjects during the skiing experiments by a 100K bits-1. PCM-FM system and recorded in digital form. Analyses of field data show the magnitude of the loading applied to the lower extremity during normal skiing often exceeds the expected, quasi-static ultimate strength of the tibia. Anterior-posterior bending of approximately 600 Nm has been recorded during skiing without binding release or injury to the test subjects. The standard heel binding design can not effectively control the bending moment in the lower extremity during skiing. Longitudinal rotation across the knee was in-phase with, though not proportional to, the applied torsion at the foot by the ski.

Athletic Injuries↗

PubMed1987

The elastic anisotropy of bone.

In modeling the anisotropic properties of hydroxyapatite (HAp), Katz found that two kinds of phenomenological relationships held among the elastic stiffness coefficients. Firstly, there are three linear combinations--(c11 + c22 + c33), (c44 + c55 + c66), (c12 + c13 + c23)--which arise naturally when computing the isotropic averages of anisotropic crystal systems over all possible spatial orientations. Secondly, the degree of elastic anisotropy in such crystal systems is characterized by two specific factors: (a) the ratio of the linear compressibility along the unique axis to that perpendicular to it, (c11 + c12 - 2c23)(c33 - c13); and (b) the ratio of the two shear moduli, c44/c66. There have been a number of experiments in recent years which have used either mechanical methods or ultrasonic techniques to measure the anisotropic elastic properties of bovine and human cortical bone. Analyses of data from these experiments show that the above relationships also play a significant role in characterizing the elastic anisotropy in bone.

Animals↗

PubMedDate not supplied

Mechanical and textural properties of pelvic trabecular bone.

So far, virtually nothing is known about the mechanical properties of pelvic trabecular bone. In this study, several techniques have been used to establish some insight in these properties. Dual-energy quantitative computer tomography (DEQCT) was used to look at the distribution of bone densities throughout the pelvic bone and nondestructive mechanical testing was used to obtain Young's moduli and Poisson's ratios in three orthogonal directions for cubic specimens of pelvic trabecular bone. The same specimens were then used for stereological measurements to obtain volume fractions and the spatial orientations of the mean intercept lengths. The combined data on the mechanical tests and the stereological measurements made it possible to calculate Young's moduli and Poisson's ratios for the specimens' principal material axes. DEQCT showed that bone densities within a pelvic bone are significantly higher in the superior part of the acetabulum, extending to the sacroiliac joint area and, secondly, in the area of the pubic symphysis. Volume fractions found for the specimens did not exceed 20%. This may be considered rather low when compared to values reported in the literature for trabecular bone of femoral or tibial origin, but the values do lie in the same range as vertebral trabecular bone. With the volume fraction as its primary predictor, values of Young's moduli were also low. For most specimens these values were not higher than 100 MPa, with an occasional peak of 250 MPa. Looking at the ratio of the highest and lowest Young's modulus or at the components of the fabric tensor, it can be concluded that pelvic trabecular bone is not highly anisotropic. On an average, Poisson's ratio was found to be closer to 0.2 rather than 0.3, which is in accordance with other studies on Poisson's ratio of trabecular bone.

Acetabulum↗

PubMed1994

A microstructural model for the elastic response of articular cartilage.

A model of articular cartilage is developed in which the continuum stiffness tensor is related to the tissue's microstructure. The model consists of bilinear elastic fibers embedded in an elastic matrix. Homogenization techniques are used to relate this level of organization to the macroscopic response of the tissue. The model includes the effects of spatial orientation of fibers, pre-stress in the fibers and matrix resulting from matrix swelling, slipping at the interface between the fibers and the matrix, fiber buckling in compression, and deformation-induced fiber reorientation. The model predicts increased axial stiffness with increasing stretch due to fiber reorientation, reduced axial and shear stiffness with slipping between fiber and matrix and a sensitivity of the tissue response to the swelling pressure in the matrix, the matrix modulus and the bonding of the fiber matrix interface.

Algorithms↗

PubMed1995

A dynamic shoulder model: reliability testing and muscle force study.

This study introduces a dynamic shoulder model, where forces were applied to individual muscles in ten cadaveric specimens. The model provided reproducible glenohumeral joint motion and thereby allowed the investigation of active, glenohumeral joint mechanics. Forces were created by servo-actuated hydrodynamic cylinders and applied to the deltoid muscle and to the rotator cuff through wire cables. Computerized regulation initiated precise, time controlled cycles of glenohumeral joint motion. The position of the glenohumeral joint in all spatial orientations was measured and recorded using an ultrasonic sensor device. Reproducibility of glenohumeral joint motion was demonstrated on the basis of five cycles of glenohumeral joint elevation. Repeatability variance of position measurements for five cycles of elevation averaged 0.80 degrees for abduction, 0.75 degrees for anteflexion and 1.36 degrees for internal rotation. Arm weight and force distribution at the shoulder musculature were estimated according to the literature. In comparison to estimated physiologic conditions, a one third increase of arm weight led to a significant (p < 0.05) decrease of elevation of 20%, a one third decrease of arm weight to an average increase of elevation of 18% (p < 0.05). Exclusion of the supraspinatus muscle caused a significant (p < 0.05) 6% decrease of elevation of the glenohumeral joint. Without force applied to the subscapularis and infraspinatus/teres minor muscles, elevation decreased 16% (p < 0.05). A decrease of glenohumeral joint elevation of 25% resulted when force was applied to the deltoid muscle alone (p < 0.05).

Adult↗

PubMed1995

Highly enantioselective capillary electrophoretic separations with dilute solutions of the macrocyclic antibiotic ristocetin A.

Ristocetin A is one of a series of structurally related amphoteric, glycopeptide, macrocyclic antibiotics. These compounds have several features that make them attractive as chiral selectors. These include spatially oriented functional groups that are known to provide the types of interactions that are conducive to enantio-recognition, a somewhat rigid "pocket" that can provide a site for hydrophobic interactions and polar, flexible arms (i.e., pendent sugar moieties) that can rotate to hydrogen bond and otherwise interact with a variety of chiral analytes. In addition, these compounds are sufficiently soluble in water, aqueous buffers and aqueous-organic solvents that are commonly used in capillary electrophoresis (CE). The use and optimization of ristocetin A as a chiral selector in CE is discussed. Over 120 racemates are resolved including a variety of N-blocked amino acids, non-steroidal anti-inflammatory compounds and a large number of biologically important compounds containing carboxylic acid groups (e.g., mandelic acid derivatives, lactic acid derivatives, folinic acid, tropic acid).

Carbohydrate Sequence↗

PubMed1989

Computer modeling from solution data of spinach chloroplast and of Xenopus laevis somatic and oocyte 5 S rRNAs.

Detailed atomic models of a eubacterial 5 S rRNA (spinach chloroplast 5 S rRNA) and of a eukaryotic 5 S rRNA (somatic and oocyte 5 S rRNA from Xenopus laevis) were built using computer graphic. Both models integrate stereochemical constraints and experimental data on the accessibility of bases and phosphates towards several structure-specific probes. The base sequence was first inserted on to three-dimensional structural fragments picked up in a specially devised databank. The fragments were modified and assembled interactively on an Evans & Sutherland PS330. Modeling was finalized by stereochemical and energy refinement. In spite of some uncertainty in the relative spatial orientation of the substructures, the broad features of the models can be generalized and several conclusions can be reached: (1) both models adopt a distorted Y-shape structure, with helices B and D not far from colinearity; (2) no tertiary interactions exist between loop c and region d or loop e; (3) the internal loops, in particular region d, contain several non-canonical base-pairs of A.A, U.U and A.G types; (4) invariant residues appear to be more important for protein or RNA binding than for maintaining the tertiary structure. The models are corroborated by footprinting experiments with ribosomal proteins and by the analysis of various mutants. Such models help to clarify the structure-function relationship of 5 S rRNA and are useful for designing site-directed mutagenesis experiments.

Animals↗

PubMed1990

Rubredoxin reductase of Pseudomonas oleovorans. Structural relationship to other flavoprotein oxidoreductases based on one NAD and two FAD fingerprints.

The oxidation of alkanes to alkanols by Pseudomonas oleovorans involves a three-component enzyme system: alkane hydroxylase, rubredoxin and rubredoxin reductase. Alkane hydroxylase and rubredoxin are encoded by the alkBFGHJKL operon, while previous studies indicated that rubredoxin reductase is most likely encoded on the second alk cluster: the alkST operon. In this study we show that alkT encodes the 41 x 10(3) Mr rubredoxin reductase, on the basis of a comparison of the expected amino acid composition of AlkT and the previously established amino acid composition of the purified rubredoxin reductase. The alkT sequence revealed significant similarities between AlkT and several NAD(P)H and FAD-containing reductases and dehydrogenases. All of these enzymes contain two ADP binding sites, which can be recognized by a common beta alpha beta-fold or fingerprint, derived from known structures of cofactor binding enzymes. By means of this amino acid fingerprint we were able to determine that one ADP binding site in rubredoxin reductase (AlkT) is located at the N terminus and is involved in FAD binding, while the second site is located in the middle of the sequence and is involved in the binding of NAD or NADP. In addition, we derived from the sequences of FAD binding reductases a second amino acid fingerprint for FAD binding, and we used this fingerprint to identify a third amino acid sequence in AlkT near the carboxy terminus for binding of the flavin moiety of FAD. On the basis of the known architecture and relative spatial orientations of the NAD and FAD binding sites in related dehydrogenases, a model for part of the tertiary structure of AlkT was developed.

Amino Acid Sequence↗

PubMed1992

DNA topology-mediated regulation of transcription initiation from the tandem promoters of the ilvGMEDA operon of Escherichia coli.

It is becoming increasingly clear that the intrinsic and protein-induced topological properties of the DNA helix influence transcriptional efficiency. In this report we describe the properties of two upstream activating regions that influence transcription from the non-overlapping tandem promoters of the ilvGMEDA operon of Escherichia coli. One 20 base-pair region between the promoter sites contains an intrinsic DNA bend that activates transcription from the downstream promoter. The other region contains an integration host factor (IHF) binding site that overlaps the upstream promoter site. IHF binding at this site represses transcription from the upstream promoter and enhances transcription from the downstream promoter. IHF also induces a severe bend in the DNA at its target binding site in the upstream promoter region. The activating property of the 20 base-pair DNA sequence located between the promoters is dependent upon the helical phasing of the sequence-directed DNA bend that it encodes. However, the IHF-mediated activation of transcription is not dependent upon the helical phasing (spatial orientation) of the upstream IHF and downstream promoter sites. The IHF-mediated activation of transcription is also uninfluenced by the presence or absence of the intrinsic DNA bend between its binding site and the downstream promoter site. These results suggest the interesting possibility that IHF activates transcription from the nearby downstream promoter simply by bending the DNA helix in the absence of specific IHF-RNA polymerase or upstream DNA-RNA polymerase interactions.

Bacterial Proteins↗

PubMed1992

X-ray structure of the DNase I-d(GGTATACC)2 complex at 2.3 A resolution.

The crystal structure of a complex between DNase I and the self-complementary octamer duplex d(GGTATACC)2 has been solved using the molecular replacement method and refined to a crystallographic R-factor of 18.8% for all data between 6.0 and 2.3 A resolution. In contrast to the structure of the DNase I-d(GCGATCGC)2 complex solved previously, the DNA remains uncleaved in the crystal. The general architecture of the two complexes is highly similar. DNase I binds in the minor groove of a right-handed DNA duplex, and to the phosphate backbones on either side over five base-pairs, resulting in a widening of the minor groove and a concurrent bend of the DNA away from the bound enzyme. There is very little change in the structure of the DNase I on binding the substrate. Many other features of the interaction are conserved in the two complexes, in particular the stacking of a deoxyribose group of the DNA onto the side-chain of a tyrosine residue (Y76), which affects the DNA conformation and the binding of an arginine side-chain in the minor groove. Although the structures of the DNA molecules appear at first sight rather similar, detailed analysis reveals some differences that may explain the relative resistance of the d(GGTATACC)2 duplex to cleavage by DNase I: whilst some backbone parameters are characteristic of a B-conformation, the spatial orientation of the base-pairs in the d(GGTATACC)2 duplex is close to that generally observed in A-DNA. These results further support the hypothesis that the minor-groove width and depth and the intrinsic flexibility of DNA are the most important parameters affecting the interaction. The disposition of residues around the scissile phosphate group suggests that two histidine residues, H134 and H252, are involved in catalysis.

Base Composition↗

PubMed1988

Water-maze learning and effects of cholinergic drugs in mouse strains with high and low hippocampal pyramidal cell counts.

Morphological differences have been found in inbred strains of mice in the number and volume of pyramidal cells in Ammon's horn of the hippocampus. Among the mouse strains surveyed, NZB/BINJ (NZB) and C57BL/10J (B10) are most divergent in both total volume and total number of neurons. These genetically derived differences were exploited to determine hippocampal involvement in the acquisition of a spatial water maze. Genetic differences in hippocampal cell number were related to the acquisition of this spatial task. Mice with small numbers of hippocampal pyramidal cells, the B10 strain, acquired a water-maze task more slowly than either NZB mice or (NZBxNZW) F1 (NZBWF) animals. In addition, strain differences in responsivity to cholinergic manipulations were found. B10 mice were more sensitive than NZB or NZBWF mice to both the disruptive effects of scopolamine and the facilitory effects of physostigmine on swim maze learning. Although other inherited differences undoubtedly exist between these strains as is apparent in other mouse lines, these data suggest a prominent role for the hippocampus in the learning of spatially oriented behavior. Furthermore, this behavior appears to be responsive to cholinergic manipulations.

Animals↗

PubMed1995

Mutations of aspartate 103 in the Hm2 receptor and alterations in receptor binding properties of muscarinic agonists.

Aspartate 103 (D103) in the third transmembrane domain of the Hm2 receptor was mutated to glutamate (D103E), asparagine (D103N), or alanine (D103A). As measured by [3H]-NMS, no significant binding was observed in D103A, while a 2-fold decrease in ligand affinity was seen in D103E and a 32-fold decrease in affinity was found in the D103N mutant. Examination of reference agonists showed greater loss of affinity in D103N than in D103E with the rank order of change being: L-607,207>carbachol>arecoline>pilocarpine>oxotremorine>McN-A-343. Of the novel 1-azabicyclo[2.2.1]-heptan-3-one oxime agonists examined, arylacetylene oximes showed little alteration in binding in either the D103E or D103N mutants, while the geometric isomers of several bicyclic aryl-ene-yne oximes showed significant changes in affinity, especially in the D103N mutant. Thus, overall size of the agonist and/or spatial orientation of the molecule within the binding pocket contribute to changes measured in binding.

(4-(m-Chlorophenylcarbamoyloxy)-2-butynyl)trimethy↗

PubMed1989

Unilateral periarcuate and posterior parietal lesions impair conditional position discrimination learning in the monkey.

Monkeys were trained on a conditional position discrimination in which the conditional cue was a light blinking at two distinct rates and the discriminanda (illuminated buttons) appeared in varying symmetrical positions of eccentricity. Unilateral arcuate, posterior parietal, or principal sulcus lesions were performed at criterion. The monkeys were tested to recovery when a homologous lesion was made contralaterally. The first of two analyses examined a period of 4 weeks following each lesion; the unit of analysis was lesions. The arcuate and parietal lesions produced impairments on both widely eccentric and central discriminanda locations; initially, virtually all responses were deflected to the ipsilateral side. There was significant improvement after the arcuate and parietal lesions from weeks 1 to 4. An analysis of total trials to criterion showed major deficits from the second arcuate and parietal lesions, with the arcuate lesion impairment being particularly severe. These results establish that a spatial concept of left vs right is seriously deranged by unilateral lesions of cortical association areas involved in spatial orientation and discrimination.

Animals↗

PubMed1995

Interactive multimedia for prenatal ultrasound training.

This demonstration project examines the utility of interactive multimedia for prenatal ultrasound training. A laser-disc library was linked to a three-dimensional (3-D) heart model and other computer-based training materials through interactive multimedia. A testing module presented ultrasound anomalies and related questions to house-staff physicians through the image library. Users were asked to evaluate these training materials on the basis of perceived instructional value, question content, subjects covered, graphics interface, and ease of use; users were also asked for their comments. House-staff physicians indicated that they consider interactive multimedia to be a helpful adjunct to their core fetal imaging rotation. During a 9-month period, 16 house-staff physicians correctly diagnosed 78 +/- 4% of unknown cases presented through the testing module. The 3-D heart model was also perceived to be a useful teaching aid for spatial orientation skills. Our findings suggest that interactive multimedia and volume visualization models can be used to supplement traditional prenatal ultrasound training. The system provides a broad exposure to ultrasound anomalies, increases opportunities for postnatal correlation, emphasizes motion video for ultrasound training, encourages development of independent diagnostic ability, and helps physicians understand anatomic orientation. We hypothesize that interactive multimedia-based tutorials provide a better overall training experience for house-staff physicians. However, these supplementary methods will require formal evaluation of effectiveness to better understand their potential educational impact.

Computer Simulation↗

PubMed1994

Pituitary grafts and behavior in mice. Effects of sex of donor and number of pituitary grafts on exploratory and learning abilities.

The effects of ectopic pituitary grafts, sex of pituitary donor, and number of isografts (one or two) were examined in intact phenotypically normal male mice of the Snell dwarf strain, using a battery of tests designed to assess exploration and learning-related behavioral processes. Results show that neither sex of pituitary donor nor number of grafts affected behavioral responses to pituitary grafts. Ectopic pituitary grafts increased the level of exploratory activities assessed in a novelty preference test and in the hole board test, facilitated the retention of a step-through type passive avoidance task--contrasting with results in rats--but did not affect spatial orientation in a spontaneous alternation and a water escape task. The results argue for a global effect of the graft, inducing increased emotional reactivity.

Animals↗