PubMed Health⌕ Search

SEARCH · PubMed Health

Results for “Intervertebral Disc”

Explore indexed PubMed citations for clinical trials, systematic reviews and public health research. Read source abstracts and follow each citation to its original PubMed record.

Quote a phrase for an exact phrase match. Source license links do not imply unrestricted reuse.

At least 1,243 records · Page 69Linked to original sources
PubMed1991

Cell culture of the intervertebral disc of rats: factors influencing culture, proteoglycan, collagen, and deoxyribonucleic acid synthesis.

To establish cell culture of the nucleus pulposus and anulus fibrosus of rat intervertebral disc, the effects of culture conditions on the growth of cells and the synthesis of DNA, proteoglycan, and collagen were studied. For cell culture of the nucleus pulposus, the use of 3-week-old rats and a medium adjusted to pH 7.0 was optimal. There was almost no difference in growth between cells in Ham's F12 medium and those in Dulbecco's Modified Eagle Medium. In cells isolated from the anulus fibrosus, a medium adjusted to pH 7.0-7.6 was preferable, but irrespective of rat age. Culture cells of the nucleus pulposus were composed of large cells with vacuoles and small polygonal cells. These cells had a slight growth activity and a fair capability of proteoglycan and collagen synthesis. Culture cells of the anulus fibrosus were composed of polygonal and spindle-shape cells, and the growth was more vigorous with the potentials for proteoglycan and collagen synthesis than the nucleus cells.

Age Factors↗
PubMed2003

Cell shape and gene expression in human intervertebral disc cells: in vitro tissue engineering studies.

The objective of the present study was to examine the relation between gene expression and the shape of human intervertebral disc cells cultured in vitro in three-dimensional (3D) scaffolds. Disc cells from 19 subjects were seeded into either a collagen sponge or collagen gel and cultured for 10 days. In situ hybridization was performed on serial sections of paraffin embedded specimens and assessed for expression of selected genes important for extracellular matrix formation: Types I and II collagen, aggrecan and chondroitin-6 sulfotransferase. Rounded cells grown in collagen gel showed expression of Types I and II collagen, aggrecan and chondroitin-6 sulfotransferase; expression of these genes was absent in spindle shaped cells. Cells in the collagen sponge that lay on the sponge margin were frequently spindle shaped; these cells expressed type I collagen, but not type II collagen, aggrecan or chondroitin-6 sulfotransferase. Results presented here provide novel data concerning disc cell gene expression with collagen 3D constructs. This information is useful for future tissue engineering studies that have the challenging goal of selectively modulating gene expression.

Adult↗
PubMed1988

Histopathology of the intervertebral disc of progressive ankylosis mice.

The intervertebral disks of progressive ankylosis mice were examined at successive periods in order to describe the pathologic lesions involved and to explore possible relationships with any known human disorder. The techniques employed in this study showed the presence of necrotic foci of calcification that ultimately obliterated the anulus fibrosus. Following the appearance of these lesions, peripheral proliferation of hyaline cartilage appeared that eventually bridged adjacent vertebral bodies, calcified, and was replaced with bone. The results of this study support the hypothesis that the progressive ankylosis mouse has a disorder affecting mineralization. Although it does not exactly resemble any known human disorder, it should be an important model for studying both crystal deposition and abnormal mineralization of hyaline cartilage and fibrocartilage since many vertebral ankylosis disorders, as well as the normal aging process, involve increased mineralization.

Animals↗
PubMed1987

The penetration of antibiotics into the normal intervertebral disc.

The role of antibiotics in the treatment of disc-space infection is controversial. This study assessed the tissue penetration of flucloxacillin and cephradine into the normal intervertebral disc after intravenous administration of a bolus dose of antibiotic. Twenty-five discs were removed from 12 adolescent patients having anterior spinal surgery to correct scoliosis; antibiotic had been administered between 30 minutes and four hours before operation. Despite high blood levels, no antibiotic could be detected by bioassay or by high-pressure liquid chromatography (h.p.l.c.) in any of the specimens from the nucleus pulposus or the annulus fibrosus.

Absorption↗
PubMed2003

Influence of fixed charge density magnitude and distribution on the intervertebral disc: applications of a poroelastic and chemical electric (PEACE) model.

A 3-dimensional formulation for a poroelastic and chemical electric (PEACE) model is presented and applied to an intervertebral disc slice in a 1-dimensional validation problem and a 2-dimensional plane stress problem. The model was used to investigate the influence of fixed charge density magnitude and distribution on this slice of disc material. Results indicated that the mechanical, chemical, and electrical behaviors were all strongly influenced by the amount as well as the distribution of fixed charges in the matrix. Without any other changes in material properties, alterations in the fixed charge density (proteoglycan content) from a healthy to a degenerated distribution will cause an increase in solid matrix stresses and can affect whether the tissue imbibes or exudes fluid under different loading conditions. Disc tissue with a degenerated fixed charge density distribution exhibited greater solid matrix stresses and decreased streaming potential, all of which have implications for disc nutrition, disc biomechanics, and tissue remodeling. It was also seen that application of an electrical potential across the disc can induce fluid transport.

Adult↗
PubMed1986

Intervertebral disc impingement syndrome in a child. Report of a case and suggested pathology.

Nerve root impingement syndrome is rare in children and adolescents. The symptomatology of the pediatric group sometimes differs from that of the adult. An accurate diagnosis is often delayed because of concerns for neoplasm, infection, and spondylolisthesis. A case report of an 11-year-old boy with symptoms and myelographic findings consistent with a herniated disc is presented. A review of the literature disclosed only four cases of herniated disc in children under the age of 12 years. Our case represents one of the youngest patients with documented intervertebral disc impingement syndrome. Rather than a herniation due to degenerative changes in the annulus, a slipping of the entire disc, and vertebral end-plate (a "slipped vertebral apophysis") may precipitate a central disc impingement in children and adolescents. This has been reported in cases of trauma previously. However, the authors think that this slipped vertebral apophysis may, in fact, represent a large number of the so-called herniated discs in adolescents. It is also suggested that this may be an identifiable syndrome with a susceptible age range, activity level and body habitus.

Child↗
PubMed1998

[Diagnostic significance of topical image of infrared thermograph on the patient with lumbar intervertebral disc herniation--a comparative study on 45 patients and 65 normal control].

OBJECTIVE: To analyse and explore the diagnostic significance of infrared thermography on the patients with lumbar intervertebral disc protrusion. METHODS: Forty-five hospitalized cases under conservative treatment (mainly manipulation) and 65 controls were sequentially selected. Both groups were examined with infrared thermograph on lower back and lower extremities as well as physical examination before and after treatment. The study statistically analysed the temperature difference between the involved and healthy parts at different areas; and also compared the change of the temperature difference after the treatment to the decreasing scores of physical examination afterward. RESULTS: The statistic result showed that the temperature difference between two sides on patients group was significantly higher than those of control group. The further analysis shows that the temperature difference at posterior femur area in the patient's group correlated significantly to the severity of clinical signs caused by nerve root irritation. CONCLUSIONS: The thermograph could only be considered as a reference in the diagnosis of lumbar disc protrusion. It is helpful to discover the severity of radical signs according to the posterior femoral region of hypo- (or hyper) thermogram.

Adult↗
PubMed1995

The large proteoglycans of the human intervertebral disc. Changes in their biosynthesis and structure with age, topography, and pathology.

STUDY DESIGN: The structure and extracellular assembly of the newly synthesized aggregating proteoglycans of the human intervertebral disc were examined using an explant culture system. OBJECTIVES: The objective was to study the changes with aging, topography, and pathology, comparing newly synthesized with endogenous proteoglycans. SUMMARY OF BACKGROUND DATA: No detailed studies of the biosynthesis of human disc proteoglycans have been previously reported. METHODS: A method of explant culture that minimizes swelling and matrix loss was used to maintain the tissue architecture. Slices of postmortem and pathologic disc tissues were incubated in medium containing polyethylene glycol at appropriate concentrations to balance the swelling pressure of the tissue. The disc slices were contained in small-pore dialysis tubing to prevent penetration of the polyethylene glycol into the tissue. The newly synthesized proteoglycans were radiolabeled with [35S]-sulphate. Proteoglycans were then extracted from the tissue slices and characterized with gel chromatographic and electrophoretic techniques. RESULTS: It was found that a single, high molecular weight proteoglycan is the major 35S-labeled synthesis product of disc cells at all ages. However, biosynthetic changes do occur: the monomer made by fetal and newborn disc cells was larger than that of adults. Furthermore, adult disc cells made other minor large 35S-labeled products, the synthesis pattern of which varied between regions. CONCLUSION: These results provide the first evidence that biosynthetic changes contribute to the age-related increase in the heterogeneity of the human disc proteoglycan population.

Adolescent↗
PubMed1999

Relationship of pain drawings to invasive tests assessing intervertebral disc pathology.

It has been found that the pain patterns in pain drawings are related to the presence of herniated disc identified by myelography. The purpose of this study was to determine whether the pattern of pain in the drawings or the type of pain indicated (aching, burning, numbness, pins and needles, stabbing) was related to the presence of symptomatic disc pathology identified by CT/discography. In a subgroup of patients who underwent myelography, the relationship of the drawings to myelographic findings was also investigated. Pain drawings were completed by 187 patients with low back and/or radicular pain who were undergoing CT/discography. The drawings were scored in two ways, first by the system described by Ransford and secondly by visual inspection. They were classified as being indicative, or not, of disc pathology. The CT/discograms were classified as disrupted, or not, and the pain responses were recorded upon injection of each disc, based on the similarity of the pain provoked to clinical symptoms. Among the 133 patients with discogenic pain confirmed by discography, 110 (82.7%) had pain drawings that were classified as indicative. Among the 45 patients without discogenic pain, 29 (64.4%) had pain drawings classified as non-indicative. Patients with discogenic pain used more symbols indicating burning pain and aching pain than did non-discogenic pain patients. Our results confirmed those reported earlier by Uden, who found a relationship between the pattern of pain in the drawings and myelographic findings. Pain drawings may be helpful in the diagnosis of symptomatic disc pathology.

Adult↗