Practical haemodialysis began with cellophane and heparin: the crucial role of William Thalhimer (1884-1961).
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A high-resolution screening procedure for the selection of mutants was developed based on the ability of some strains of Mycobacterium smegmatis to produce pigment in the light. Out of 23 000 colonies investigated, 17 auxotrophic mutants were isolated. Specific growth factor requirements for the mutants and the proportion of revertants were determined.
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The biocompatibility of cuprammonium rayon (Cu), cellulose acetate, polysulfone (Ps) and polyacrylonitrile hollow-fiber dialyzers and a polycarbonate-polyether flat plate dialyzer has been investigated. The Cu dialyzer resulted in more complement activation and a greater degree of leukopenia than the others, while the Ps hollow-fiber dialyzer appeared to be the most biocompatible of the membrane equipment in this study. These results were confirmed by in vitro evaluations and microscopic examinations of the different dialyzers.
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