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Environmental pollutant tributyltin promotes Th2 polarization and exacerbates airway inflammation.

It has been shown that a relatively high dose of tributyltin (TBT), which is recognized as a particularly notable environmental pollutant, exerts immunotoxic effects such as thymic atrophy via induction of T cell apoptosis. However, the effect of low doses of TBT on the immune responses remains unknown. Here we show that environmentally relevant doses of TBT promoted strong Th2 polarization via suppression and augmentation of Th1 and Th2 development, respectively, from naive CD4(+) T cells primed with anti-CD3 and splenic antigen-presenting cells (APC). TBT-induced Th2 polarization was indirect, working through APC via suppression of IL-12 production by macrophages/DC and the augmentation of IL-10 production by B cells. Th2 polarization was also induced in mice treated with TBT and immunized with OVA or infected with Nippostrongylus brasiliensis. Furthermore, airway inflammation in mice sensitized and challenged with OVA was exacerbated by the administration of TBT with concomitant augmentation of Th2-type immunity. Our results highlight the fact that an important environmental pollutant TBT may present significant risk for the induction of allergic diseases via promotion of Th2 polarization.

Animals↗

Environmental pollution affects genetic diversity in wild bird populations.

Many common environmental pollutants, together with nuclear radiation, are recognized as genotoxic. There is, however, very little information on pollution-related genetic effects on free-living animal populations, especially in terrestrial ecosystems. We investigated whether genetic diversity in two small insectivorous passerines, the great tit (Parus major) and the pied flycatcher (Ficedula hypoleuca), was changed near point sources of heavy metals (two copper smelters) or radioactive isotopes (nuclear material reprocessing plant). We measured concentration of heavy metals and nucleotide diversity in mitochondrial DNA in feather samples taken from nestlings in multiple polluted areas and at control sites. In both species, heavy metal concentrations - especially of arsenic - were increased in feathers collected at smelter sites. The P. major population living near a smelter showed significantly higher nucleotide diversity than a control population in an unpolluted site, suggesting increased mutation rates in a polluted environment. On the contrary, F. hypoleuca showed reduced nucleotide diversity at both smelter sites but increased nucleotide diversity near the source of radioactivity. Our results show that heavy metal pollution and low level nuclear radiation affect the nucleotide diversity in two free-living insectivorous passerines. We suggest that the different response in these two species may be due to their different ability to handle toxic compounds in the body.

Animals↗

Environmental pollutants and disease in American children: estimates of morbidity, mortality, and costs for lead poisoning, asthma, cancer, and developmental disabilities.

In this study, we aimed to estimate the contribution of environmental pollutants to the incidence, prevalence, mortality, and costs of pediatric disease in American children. We examined four categories of illness: lead poisoning, asthma, cancer, and neurobehavioral disorders. To estimate the proportion of each attributable to toxins in the environment, we used an environmentally attributable fraction (EAF) model. EAFs for lead poisoning, asthma, and cancer were developed by panels of experts through a Delphi process, whereas that for neurobehavioral disorders was based on data from the National Academy of Sciences. We define environmental pollutants as toxic chemicals of human origin in air, food, water, and communities. To develop estimates of costs, we relied on data from the U.S. Environmental Protection Agency, Centers for Disease Control and Prevention, National Center for Health Statistics, the Bureau of Labor Statistics, the Health Care Financing Agency, and the Practice Management Information Corporation. EAFs were judged to be 100% for lead poisoning, 30% for asthma (range, 10-35%), 5% for cancer (range, 2-10%), and 10% for neurobehavioral disorders (range, 5-20%). Total annual costs are estimated to be $54.9 billion (range $48.8-64.8 billion): $43.4 billion for lead poisoning, $2.0 billion for asthma, $0.3 billion for childhood cancer, and $9.2 billion for neurobehavioral disorders. This sum amounts to 2.8 percent of total U.S. health care costs. This estimate is likely low because it considers only four categories of illness, incorporates conservative assumptions, ignores costs of pain and suffering, and does not include late complications for which etiologic associations are poorly quantified. The costs of pediatric environmental disease are high, in contrast with the limited resources directed to research, tracking, and prevention.

Asthma↗

White adipose tissue: storage and effector site for environmental pollutants.

White adipose tissue (WAT) represents a reservoir of lipophilic environmental pollutants, especially of those which are resistant to biological and chemical degradation - so-called persistent organic pollutants (POPs). Large amounts of different congeners and isomers of these compounds exhibit a variety of adverse biological effects. Interactions among different classes of compounds, frequently with opposing effects, complicate hazard evaluation and risk assessment. WAT is the key organ for energy homeostasis and it also releases metabolites into the circulation and adipokines with systemic effects on insulin sensitivity and fuel partitioning in muscles and other tissues. Its beneficial role is lost in obesity when excessive accumulation of WAT contributes to severe diseases, such as diabetes. POPs may crossroad or modulate the effect of endogenous ligands of nuclear transcription factors, participating in differentiation, metabolism and the secretory function of adipocytes. These mechanisms include, most importantly: i) endocrine disrupting potency of POPs mixtures on androgen, estrogen or thyroid hormone metabolism/functions in WAT, ii) interference of dioxin-like chemicals with retinoic acid homeostasis, where impact on retinoid receptors is expected, and iii) interaction with transcriptional activity of peroxisome proliferator-activated receptors is likely. Thus, the accumulation and action of POPs in WAT represents a unitary mechanism explaining, at least in part, the effects of POPs in the whole organism. By modulating WAT differentiation, metabolism and function, the POPs could affect not only the physiological role of WAT, but they may also influence the development of obesity-associated diseases.

Adipogenesis↗

[Decrease in environmental pollution and changes in biological indicators in occupational exposure to lead].

The Authors examine the variations of the levels of PbB and EPP in 39 workers at known lead exposure and evaluate the capacity of these parameters to follow the measured decreases of the environmental pollution. They conclude that the variations of the mean PbB values are well related to environmental pollution and that the diagram on the probability paper of median values and their corresponding standard deviations allows to calculate the number of exposed workers with biological values above a fixed limit.

Adult↗

Concern about environmental pollution: how much difference do race and ethnicity make? A New Jersey case study.

A survey conducted among 1,513 residents of New Jersey during March-May 2004 showed that non-Hispanic black, non-Hispanic white, and English-speaking Hispanic Americans were significantly more concerned about environmental pollution problems than were Asian Americans and Spanish-language Hispanic Americans. For example, an average of > 40% of the first three groups was very concerned about New Jersey's environmental problems, compared with 15% of the last two populations. There were also racial/ethnic differences among these groups in their desire for government action to protect the environment and in their personal support of the environmental movement. Regression analyses suggest that the 1970s and 1980s model of core support for environmental protection from white, female, young, educated, and politically liberal people has largely, but not completely, continued among non-Hispanic white, non-Hispanic black, and English-language Hispanic populations. But these demographic pointers do not hold for Asian and Spanish-language Hispanic Americans, except indicating more support among the more formally educated. The last two groups are the two fastest-growing subpopulations in the United States, and although acculturation may slowly increase their concern about environmental pollution, it is more prudent for proponents of environmental protection not to wait and instead to try to better understand the environmental perceptions of these groups.

Adult↗

Environmental pollution in the Gulf of Guinea--a regional approach.

Environmental pollution in the Gulf of Guinea (GOG) coastal zone has caused eutrophication and oxygen depletion in the lagoon systems, particularly around the urban centres, resulting in decreased fish (reproduction) levels and waterborne diseases. A pollution sources assessment was undertaken by six countries in the region as a first step in defining a region-wide Environmental Management Plan. Results show that households produce 90% of solid waste. Industry, however, is responsible for substantial amounts of hazardous waste, specifically the Nigerian petroleum industry. The latter is also responsible for the spilling of large amounts of oil. BOD load from industrial effluents is slightly larger than domestic loads in the industrialised coastal zone. Wastewater treatment systems are either absent or inadequate. Apart from large-scale gas flaring in Nigeria, air pollution, in terms of COx, HC, NOx and SO2 emissions, is contributed mainly by traffic. Particulates, originate mainly from industries and domestic biomass burning.

Africa↗

Effects of xenoestrogenic environmental pollutants on the proliferation of a human breast cancer cell line (MCF-7).

A human breast cancer cell line (MCF-7) was used to develop an in vitro screening assay for the detection of xenoestrogenic environmental pollutants. MCF-7 cells were cultured in DMEM containing 5% fetal bovine serum (FBS). An estrogenic response was defined as an increase in the frequency of proliferating MCF-7 cells, and was measured using a thymidine analog, bromodeoxyuridine, and flow cytometry. Di-2-ethylhexyl phthalate (DEHP) and 4-n-nonylphenol (4-n-NP) were used as model chemicals. The proliferation rate of S-phase cells after 24 h of exposure to various concentrations of 17beta-estradiol and to model compounds was compared with a positive and a negative control, containing 1 nM 17beta-estradiol and 0.1% ethanol, respectively. DEHP and 4-n-NP increased the frequency of proliferating MCF-7 cells in a dose-dependent manner. The lowest concentration that significantly increased the proliferation of MCF-7 cells was 10 microM for DEHP and 1 microM for 4-n-NP. The results showed that the assay is accurate and quick to perform. It may prove a valuable tool for screening potential estrogen-mimicking environmental pollutants.

Breast Neoplasms↗

Engineering dioxygenases for efficient degradation of environmental pollutants.

Dioxygenases have recently been engineered to improve their capabilities for environmental pollutant degradation. The techniques used to achieve this include in vitro DNA shuffling and subunit or domain exchanges between dioxygenases of different bacterial origins. Such evolved enzymes acquire novel and enhanced degradation capabilities of xenobiotic compounds, such as polychlorinated biphenyls, trichloroethylene and a variety of aromatic compounds. Hybrid strains in which the evolved genes are integrated into the chromosomal operons exhibit efficient degradation of xenobiotic chlorinated compounds.

Amino Acid Sequence↗

Ranking the risks of 12 major environmental pollutants that occur in Japan.

The risks posed by 12 major environmental pollutants in Japan were evaluated and ranked on the same scale. These were arsenic, benzene, cadmium, chlordane, chlorpyrifos, DDTs, dioxins, formaldehyde, methylmercury, radon, toluene, and xylenes. Approximately half of these substances are carcinogenic while the other half are non-carcinogenic. We applied a risk estimation framework that can evaluate both cancer and non-cancer risks on the same scale. The framework consists of two parts: the calculation of the probability of adverse health effects, and the evaluation of the severity of the effects. In order to calculate the probability of adverse health effects, individual variabilities in exposure level, metabolizing rate, and sensitivity were taken into account. Loss of life expectancy (LLE; days) was used as a measure of severity of the adverse health effects and of the resulting risk level. The risk level of the substances in terms of LLE ranged from approximately 0.01 to 10 days. The risks from radon and formaldehyde were found to be the highest, while those from DDT and chlordane were the lowest. Our findings also suggested that the risk levels posed by non-carcinogenic substances were comparable to those posed by carcinogenic substances.

Environmental Pollutants↗

Effect of environmental pollutants on hepatocellular function in rats: 3-methylcholanthrene and Aroclor-1254.

Environmental pollutants, Aroclor-1254 (PCB) and 3-methylcholanthrene (MC), were employed in this study to investigate some aspects of the induction of hepatic drug metabolism in rats. PCB and MC treatments increased 7-ethoxyresorufin and 7-ethoxycoumarin O-deethylase activities related to cytochrome P-448. Cytochrome P-450 reductase activity was increased by PCB while no effect was observed by MC treatment. Pretreatment with PCB resulted in approximately 50% increase in the phospholipid content of the microsomes whereas MC caused no change. Liver microsomal cholesterol content was decreased while triglycerides were increased by PCB. The ratio between saturated and unsaturated fatty acids (saturation index) decreased in the total microsomes and phospholipids with PCB treatment, whereas MC did not alter the ratio, except that the major effect of MC was observed in the acyl derivatives of microsomal phosphatidylethanolamine. It is proposed that the uniaxial rotation and mobility of hemoproteins may be restricted by an increase in the saturation index of the membrane, while a decreased index may facilitate contact with reductases for electron transfer by enhanced membrane fluidity. The decreased saturation index after treatment with MC may play a role in carcinogenicity by triggering induction of free radicals.

Animals↗

Estrogen can protect splenocytes from the toxic effects of the environmental pollutant 4-tert-octylphenol.

Four-tert-octylphenol (OP), an environmental pollutant, exerts apoptotic effects on cultured mouse splenocytes. Although OP binds to estrogen receptors, these apoptotic effects are not exerted by 17 beta-estradiol (E). It remained possible that OP might bind to estrogen receptors and subsequently exert apoptotic effects not exerted by E after it binds to the same receptors. It also remained possible that E-primed splenocytes might respond to OP differently than splenocytes not exposed to E. Thus, we investigated OP and E interactions on the viability of mouse splenocytes in culture. The total number of splenocytes (cells stained and not stained with trypan blue) was not altered or altered slightly after incubation with any agent for 24 h. Incubation of splenocytes in medium containing 5 x 10(-5) or 5 x 10(-7) M OP decreased the percentage of viable cells by only approx 47% and 25%, respectively. The addition of 0.8 x 10(-5) to 0.8 x 10(-9) M E to cultures was without effect or decreased the percentage of viable cells by only approx 5%. The addition of these concentrations of E simultaneously with or at 2 h after the addition of 5 x 10(-5) M or 5 x 10(-7) M OP to cultures did not interfere with the OP-induced decreases in cell viability. By contrast, incubation of splenocytes in medium containing E for 2 h prior to the subsequent addition of either dose of OP blocked the OP-induced decreases in cell viability in a dose-response manner. There was a marked reduction in the percentage of viable cells (70%) when splenocytes were incubated with 0.5 x 10(-5) M dexamethasone. The addition of 0.8 x 10(-5) M E at 2 h prior to the addition of dexamethasone did not prevent the decreased cell viability. Incubation of cells in medium with 0.8 x 10(-5) M testosterone caused a small decrease in splenocyte viability similar to that observed with E. However, unlike E, the addition of testosterone at 2 h prior to the addition of 5 x 10(-5) M OP did not prevent the OP-induced decrease in cell viability. These data suggest the presence of estrogen receptors in some splenocytes. They also suggest that if OP binds to these estrogen receptors or other receptors in the absence or initial presence of E, the resulting effect is toxic to the cells. By contrast, exposure of splenocytes to E prior to their exposure to OP can prevent the toxicity of OP.

Animals↗

Energy needs versus environmental pollution: a reconciliation?

In this article I have presented, for discussion, a proposed system for energy generation by which the principal sources of environmental pollution by power plants could be eliminated. For stationary power plants the concept appears feasible technically and, according to my " horseback estimates," perhaps economically as well, depending upon the economic value of the by-products of sulfur, CO(2), water, and possibly nitrogen, and upon the price we are willing to pay for a clean environment .Thus, a more thorough engineering and economic analysis to explore these and other factors in greater depth seems warranted. In the case of turbine-driven vehicles, the technical and economic feasibility of widespread distribution and handling of the fuel constitutes a serious question, but one which deserves equally serious consideration before the possibility is discounted. The reports of the cited study panels notwithstanding, the technology required for the proposed system exists today, with one exception. This exception (which is not essential for trial of the system but will be required for its complete fruition) is the development of a nuclear reactor for the prime purpose of delivering process heat for the steam reforming of natural gas and, ultimately, for gas production from coal in a continuous process, such as those discussed by Pieroni et al. (16). Today's intermittent processes of coking and gas production are both archaic and themselves large sources of atmospheric pollution, and a development program aimed at advancing the technology of the coal industry in this regard would seem long overdue. The report of the PSAC Environmental Pollution Panel recommended "demonstration of the feasibility and economy of new developments for abating or controlling pollution through their use at Federal installations" and suggested the coalburning TVA power plants as a likely place for such demonstration. This suggestion is doubly appropriate since the TVA is in a region of subnormal " atmospheric ventilation" (8). By design these plants are adjacent to the AEC's Oak Ridge National Laboratory, and such a location would seem ideal for an experiment on the wedding of nuclear and fossil sources of energy. In comments on a preliminary draft of this article, proponents of "conventional " nuclear power pointed out that such power is hard to beat on the basis of cost, and that dissipation of heat to the air by way of cooling towers can also be accomplished in conventional plants (17). These observations are individually correct but not compatible: the low power costs cited are for very large plants [of the order of 1000 mega-watts lectrical) and larger], and the costs of cooling towers and associated equipment needed to dissipate such large amounts of heat [of the order of 2000 megawatts (thermal)] to air from a closed cycle would offset the power cost advantage of the large plant. In regard to the proposed use of nuclear process heat, Weinberg (20) has expressed doubt that much advantage can be derived from this approach because the temperatures involved are too high for low-cost reactors, and heat transfer from surfaces could involve materials problems. In the case of gas production, this is indeed an anticipated problem-not a technologically insuperable one, but a problem of reducing the cost of the materials required (16). Indeed, Weinberg himself has mentioned this possible use of nuclear heat in a recent publication discussing the steam reforming of coal to liquid fuel(21). Also, an improved process for synthesizing methane from lignitec has recently been reported (22). Since the earlier studies date back a decade, a new look at the problems and costs involved relative to the benefits to be derived (not the least of which could be new vigor for the coal industry) would seem to be in order. In the case of steam reforming of natural gas, the temperature level (about 1500 degrees F) is such that the technology is available today, and a process-heat-reactor design study could be initiated without awaiting further developments.

Air Pollution↗

[Congenital developmental defects in children and environmental pollution].

In one of the southern regions of the Ukraine, analysis was made over the recent three years of the genetic monitoring data on the incidence and structure of congenital developmental abnormalities in the newborn as related to the status of environmental pollution (the content of dust, carbon black, carbon and nitrogen oxides, nitrogen and sulfur dioxides, hydrogen sulfide, formaldehyde, phenol, fluorine compounds in the atmospheric air; the content of toxic chemicals and pesticides in foods, soil, water and feeds) as well as to the radiation situation. In the region under study, areas with varying degree of environmental pollution were distinguished. That was mainly done in terms of the characteristics of the atmospheric air and (to a less degree) varying annual dose of external radiation on conversion to one inhabitant. Analysis of the genetic monitoring has demonstrated the prevalence of congenital abnormalities, in the genesis of which the mutation component was of paramount importance, to be significantly higher in an ecologically unsafe area. The data obtained can be used in the regional programs aimed at the improvement of the environment, a reduction of the influence of exogenous risk factors implicated in the formation of congenital developmental abnormalities in children.

Abnormalities, Drug-Induced↗

Proteomics in environmental pollution research: Advances, challenges, and future directions.

Environmental proteomics has emerged as a powerful approach for elucidating the molecular mechanisms underlying pollutant-induced biological effects. Although this field has developed rapidly, the systematic review of recent proteomics applications in environmental pollution research remains limited. This review explored the emerging roles of toxicoproteomics in biomarker discovery and mechanistic elucidation, as well as ecotoxicoproteomics in ecological risk assessment and bioremediation strategies. Here, we review the field, highlighting recent trends such as the integration of proteomics with genomics, transcriptomics, and metabolomics to provide a comprehensive view of biological responses to environmental stressors. We further discuss the growing application of artificial intelligence in improving proteomics data interpretation and accelerating biomarker discovery. In addition, recent technological advances in environmental proteomics are highlighted, including next-generation tissue microarray proteomics, nanoscale proteomics, single-cell proteomics, and spatial proteomics. Despite its potential, proteomics faces challenges, such as high operational costs, computational complexity in analysis, and technical limitations in low-abundance protein detection. We propose that the convergence of proteomics with artificial intelligence and multi-omics approaches offers promising solutions to these challenges, enhancing the practical application of proteomics in environmental monitoring and risk assessment.

Proteomics↗

[Environmental pollution and attendant contamination of agricultural domestic animals. Practical conditions in the provinces].

Cattle are exposed to pollution by air, water, grass or soil. Exposure to environmental pollution is mainly through the eating of contaminated grass or feed. Case studies in the province of South-Holland show the importance of risk evaluation not just for human health but also for the health of cattle. The sequence "environment-cattle-public health" needs attention. The economic effects of pollution on agriculture may be considerable.

Animal Welfare↗