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At least 127 records · Page 7Linked to original sources
PubMed1964

STAPHYLOLYTIC ENZYME FROM CHALAROPSIS: MECHANISM OF ACTION.

The staphylolytic enzyme recently isolated from cultures of a Chalaropsis species by Hash is shown to be an acetylmurainidase that cleaves all the glycosidic linkages of N-acetylmuramic acid and N,O-diacetylmuramic acid in the cell wall of Staphylococcus aureus strain Copenhagen. It is similar in specificity to the "32 enzyme" from Streptomyces albus but it differs from egg-white lysozyme whose activity is inhibited by the presence of O-acetyl groups.

Cell Wall↗
PubMed1963

Polysaccharide produced by the genus Pullularia. I. Production of polysaccharide by growing cells.

A dextranlike polysaccharide was found to be produced on substrates of sucrose, maltose, glucose, and fructose by growing cells of various strains of the genus Pullularia. The polysaccharide, obtainable in amounts as large as 2 to 3 g per 100 ml of culture medium using various carbohydrates as the carbon source, was soluble in cold water but not in 50% alcohol. The polysaccharide obtained had a alpha(d) = +197.5 degrees (c = 0.2 in water), and its molecular weight, determined by the light-scattering method, was found to be approximately 250,000.

Carbohydrates↗
PubMed1964

FLUORESCENT-ANTIBODY STUDIES OF THE MYCELIAL FORM OF HISTOPLASMA CAPSULATUM AND MORPHOLOGICALLY SIMILAR FUNGI.

Kaufman, Leo (Communicable Disease Center, Atlanta, Ga.), and Brenda Brandt. Fluorescent-antibody studies of the mycelial form of Histoplasma capsulatum and morphologically similar fungi. J. Bacteriol. 87:120-126. 1964.-Fluorescent-antibody reagents were developed to differentiate the mycelial form of the pathogen, Histoplasma capsulatum, from several morphologically similar but saprophytic species of the genera Sepedonium and Chrysosporium. Cross-staining and adsorption procedures revealed antigenic relationships among certain species of these three genera and other fungi. The significance of these observations to the diagnostician and taxonomist is discussed.

Coloring Agents↗
PubMed1964

FORMATION OF "PROTOPLASTS" OF STAPHYLOCOCCUS AUREUS WITH A FUNGAL N-ACETYLHEXOSAMINIDASE.

Hash, John H. (Lederle Laboratories, Pearl River, N.Y.), Marcia Wishnick, and Philip A. Miller. Formation of "protoplasts" of Staphylococcus aureus with a fungal N-acetylhexosaminidase. J. Bacteriol. 87:432-437. 1964.-"Protoplasts" of Staphylococcus aureus were obtained when cells were treated with a fungal N-acetyl-hexosaminidase in hypertonic sucrose solution. The "protoplasts" were sensitive to osmotic shock, and were free of amino sugars and rigid cell walls. They were unable to regenerate cell walls and form colonies. They may be true protoplasts. Osmotic barriers in the "protoplasts" were preserved, and the "protoplasts" incorporated C(14)-l-alanine at about 60% of the rate of intact cells.

Alanine↗
PubMed1964

BIOSYNTHESIS OF YEAST CAROTENOIDS.

Simpson, Kenneth L. (University of California, Davis), T. O. M. Nakayama, and C. O. Chichester. Biosynthesis of yeast carotenoids. J. Bacteriol. 88:1688-1694. 1964.-The biosynthesis of carotenoids was followed in Rhodotorula glutinis and in a new strain, 62-506. The treatment of the growing cultures by methylheptenone, or ionone, vapors permitted observations of the intermediates in the biosynthetic pathway. On the basis of concentration changes and accumulation in blocked pathways, the sequence of carotenoid formation is postulated as phytoene, phytofluene, zeta-carotene, neurosporene, beta-zeacarotene, gamma-carotene, torulin, a C(40) aldehyde, and torularhodin. Torulin and torularhodin were established as the main carotenoids of 62-506.

Aldehydes↗