Stereospecificity of monoamine oxidase inhibitors.
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1-Benzyl-2-methyl-5-methoxytryptamine (BAS) inhibits monoamine oxidase in man. This finding confirms the findings of Woolley in mice. Since BAS, a tranquilizing agent, and Marsilid, a psychoenergizer, are both monoamine oxidase inhibitors, doubt is cast upon the hypothesis, that the stimulatory effect of Marsilid is due to its ability to inhibit monoamine oxidase.
1-Benzyl-2-methyl-5-methoxytryptamine has been reported, on the basis of indirect evidence, to inhibit monoamine oxidase. More direct experiments, however, demonstrate that the drug is devoid of the ability to block monoamine oxidase in brain in vitro or in vivo.
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Experiments in cats infused with 5 micromole of dl-adrenaline-2-C(14) showed that blockade of either monoamine oxidase or catechol-O-methyltransferase is largely compensated for by the activity of the intact enzyme system; combined blockade of both enzyme systems results in the formation of a new adrenaline catabolite and in the increased production of acidic, mainly conjugated, catabolites, the identity of which remains to be established.
A histochemical method for demonstration of monoamine oxidase activity was found to be almost as sensitive as a biochemical method when used for revealing total inhibition of monoamine oxidase in rat tissues after administration of beta-phenyl-isopropyl hydrazine. The histochemical method is of special value in the study of monoamine oxidase inhibition in the complex structures of the brain.
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