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At least 127 records · Page 7Linked to original sources
PubMed1976

Use of auxotrophic mutants to isolate LL- or DD-isomers of 2,6-diaminopimelic acid.

Pseudomonas aeruginosa PAC7 (a mutant deficient in diaminopimelate epimerase), excreted diaminopimelate (solely LL-isomer) after growth in a minimal medium plus lysine with succinate as carbon source. More diaminopimelate was excreted when bacteria were transferred at the end of the exponential phase of growth into fresh minimal medium without lysine but supplemented with pyrivate and additional (NH4)2SO4. The excreted LL-isomer was isolated from the culture filtrate by ion-exchange chromatography and purified by crystallization (1.7 g/9 1 culture). A diaminopimelate-requiring mutant of Bacillus megaterium NCIB7581 grew on LL- and/or meso-diaminopimelate but not on the DD-isomer. This mutant was used to isolate the DD-idomer from a mixture of synthetic LL- and DD-diaminopimelate. It was grown in a minimal medium containing glycerol as carbon source and LL- plus DD-diaminopimelate at a growth-limiting concentration (300 mg l-1); when growth stopped, the DD-diaminopimelate that remained in the culture was isolated and crystallized (1.0 g/II l culture).

Bacillus megaterium↗
PubMed1971

On the process of cellular division in Escherichia coli. VI. Use of a methocel-autoradiographic method for the study of cellular division in Escherichia coli.

A technique allowing chain formation by Escherichia coli for the analysis of the segregation of certain cellular components by autoradiographic methods has been developed. Deoxyribonucleic acid labeled with (3)H-thymidine does not appear to segregate preferentially with one or another of the bacteria. Membrane lipids labeled with either (3)H-oleate or (3)H-glycerol are evenly distributed among all progeny cells. A similar pattern of distribution of cell wall material is observed when (3)H-diaminopimelate is used as the marker.

Autoradiography↗
PubMed1972

Mannitol sensitivity.

The addition of mannitol to cultures of Salmonella typhimurium mutants missing mannitol-1-phosphate dehydrogenase causes stasis or lysis. Mannitol-1-phosphate accumulates intracellularly to concentrations of 20 mm. The incorporation of acetate into lipid is inhibited before cell wall, protein, or nucleic acid synthesis.

Acetates↗
PubMed1962

Ribonucleic acid in a "membrane" fraction of Escherichia coli and its relation to cell-wall synthesis.

Suit, Joan C. (The University of Texas M. D. Anderson Hospital and Tumor Institute, Houston). Ribonucleic acid in a "membrane" fraction of Escherichia coli and its relation to cell-wall synthesis. J. Bacteriol. 84:1061-1070. 1962.-A small amount of ribonucleic acid (RNA) was found in a "membrane" fraction prepared from osmotically sensitized Escherichia coli. It exhibited an elevated metabolic activity in that it attained the highest specific activity of any RNA in subcellular fractions of logarithmic-phase cells or spheroplasts prepared from logarithmic-phase cells which had been allowed to incorporate P(32) briefly. The metabolic activity of this RNA, in terms of P(32) incorporation, was found to be independent of cell-wall synthesis in the diaminopimelic acid (DAP)-less mutant, E. coli W 173-25, but was inhibited by penicillin in both this strain and in E. coli B. The latter effect is considered to be a result of other complex inhibitions of cellular metabolism by the antibiotic. The development of sensitivity to osmotic shock, capability of recovery, and synthesis of macromolecules in penicillin-treated and DAP-starved cultures, under these conditions, is described.

Cytoplasm↗
PubMed1965

CHEMICAL COMPOSITION OF CELL-WALL PREPARATIONS FROM STRAINS OF VARIOUS FORM-GENERA OF AEROBIC ACTINOMYCETES.

Cell-wall preparations were made from more than 140 strains of aerobic actinomycetes representing most of the form-genera that have been proposed. All cell-wall preparations contained as major constituents glucosamine, muramic acid, alanine, and glutamic acid. In addition, cell-wall preparations from various types of streptomycetes and strains of Microëllobosporia contained glycine and ll-alpha,epsilon-diaminopimelic acid; those from strains of most Actinoplanaceae and micromonosporae contained glycine and meso-alpha-epsilon-diaminopimelic acid; those from strains of Thermoactinomyces, Microbispora, Dermatophilus, and nocardiae of the madurae-pelletieri group contained meso-alpha,epsilon-diaminopimelic acid; and those from strains of Thermomonospora, Micropolyspora, and most nocardiae contained meso-alpha,epsilon-diaminopimelic acid, arabinose, and galactose. All the strains used were also studied morphologically.

Actinomycetales↗
PubMedDate not supplied

Preliminary studies of a dicarboxylic acid as an energy substrate in man.

Azelaic acid (Az), a straight saturated chain nine carbon dicarboxylic acid, was administered in saline form to six healthy male volunteers by iv route. Serum levels of Az and urinary amounts of both azelaic and pimelic (C7) acids were measured by an improved gas liquid chromatographic method. Stoichiometric analysis of Az metabolism was compared with that of glucose and palmitic acid. The respiratory quotient (RQ) as well as the ATP/CO2 ratio of Az were quite similar to that of palmitic acid. Therefore, Az oxidation is associated with a low cost of ATP synthesis in terms of carbon dioxide production. At the infusion rate used (7.5 g/hr) more than 50% of the administered dose was excreted in the urine. However, the remaining portion was cleared from the plasma in 200 min suggesting an uptake by body tissues which was also confirmed by indirect calorimetric analysis.

Adenosine Triphosphate↗