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At least 127 records · Page 7Linked to original sources

Differentiation of pathogenic and non-pathogenic serotype 1 Marek's disease viruses (MDVs) by the polymerase chain reaction amplification of the tandem direct repeats within the MDV genome.

There are no simple, direct methods to reliably distinguish oncogenic serotype 1 Marek's disease viruses (MDVs) from their attenuated variants. The present study was an attempt to apply polymerase chain reaction (PCR) to develop a rapid and sensitive assay for the presence of the MDV genome. PCR oligos were chosen to flank the 132-base-pair tandem direct repeats in the serotype 1 MDV genome. The PCR reaction was specific for serotype 1 MDVs, amplifying fragments corresponding to one to three copies of the tandem repeats present in Md11/8, JM/102W, and GA viruses. A high-molecular-weight DNA smear was observed when the DNA from an attenuated Md11/100 was PCR-amplified. Use of the PCR technique allowed the detection of two copies of the 132-base-pair repeat in the DNA extracted from MDV-induced lymphomas removed from two chickens. No DNA was amplified from the DNA extracted from lymphomas induced by either an avian leukosis virus (RAV-1) or reticuloendotheliosis virus (chick syncytial virus).

Animals

Studies concerning the hemagglutinant activity of some pathogenic and opportunistic pathogenic strains of genus Vibrio.

1606 bacterial strains, belonging to Vibrio genus (189 V. cholerae 0 : 1; 1091 V. cholerae nongroup 0 : 1 and 205 V. halophilic strains) of different sources of isolation, were studied, concerning their hemagglutinating behaviour to 5 different animal red blood cells (human, bovine, chicken, African green monkey and guinea pig) in mannose/fucose presence/absence. The study aimed to establish the spectrum of their hemagglutinating activity as well as any possible correlation between the source of isolation, serogroup etc and the HA-type/subtype. Mannose/fucose sensitive as well as mannose/fucose resistant hemagglutinins were exhibited by the different tested strains. As unknown behaviour, a noticeable hemagglutination only in the carbohydrate presence was recorded. The HA-types and subtypes in 861 V. cholerae nongroup 0 : 1 tested strains are presented.

Animals

[Pathogens of urinary tract infections in ambulatory care--resistance status and pathogen spectrum].

320 bacterial strains, isolated in January 1988 in the course of routine diagnostic investigations from patients with urinary tract infections, were compared with 594 strains isolated in 1983. Samples originated from outpatients in Tyrol and Vorarlberg. Most of the strains examined proved to be sensitive to oral chemotherapeutics. No increase in resistance to standard agents was observed from 1983 to 1988; the most frequently isolated organisms are still E. coli and enterococci. In contrast to the situation in hospitals, bacteriological investigation of urinary samples is not mandatory for outpatients with acute urinary tract infection.

Anti-Bacterial Agents