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[Serum antibody levels of cytomegalovirus, herpes simplex type I and adenovirus in patients with benign or malignant prostatic neoplasms].

The serum antibody titers of cytomegalovirus (CMV), Herpes Simplex Type I (HSV-L) and Adenovirus (ADV) were detected by complement fixation technique in 40 patients with benign hyperplasia of prostate (BPH), and prostatic carcinoma. The control group consisted of healthy 20 military personnel. While, antibody levels of CMV and HSV-I was found to be elevated in BPH, the titers of ADV was elevated in protatic cancer patients. The verify the relation between virus-prostatic cancer relation further clinical and laboratory researchers are needed.

Adenoviruses, Human↗

[Prostatic neoplasms: clinical and biological aspects, histopathologic patterns].

Medical imaging has an important role in the diagnosis and treatment options of prostate cancer (TRUS guided biopsies, MRI). The knowledge of the different types of cancers, their preferred topography, imaging features, extension pattern, and also the important items that may help the surgical procedure (or any other treatment) are as many crucial key points for optimal management of patients.

Adult↗

[Low-dose radiotherapy of localized prostate neoplasms: How low is high enough?].

BACKGROUND: According to the American Patterns of Care Studies, at least 70 Gy are required to achieve local control of large or undifferentiated carcinomas of the prostate. More recent data on repeated measurements of the prostate-specific antigen (PSA) cast doubt on the radiocurability of tumors with markedly elevated PSA. PATIENTS AND METHODS: With a retrospective analysis, the treatment results of local radiotherapy to mid-sized pelvic volumes with a median dose of 66 Gy (1979 to 1988, n = 118) are compared to the outcome after radiotherapy to small prostatic volumes with a median dose of 70.2 Gy (1989 to 1992, n = 126). RESULTS: Overall survival at 5 years was 65.9% and 82.3%, respectively. Patients treated at a later time had the same life expectancy as expected for the normal population. Distant disease-free survival was identical in both groups (70.4 and 74.3% at five years). Local control could not be assessed by digital rectal examination in a large part of the patients. However, in 50 patients without any pretreatment, the course of PSA was followed. Pretreatment values of > 30 ng/ml were highly predictive for "biochemical relapse" (rising values) within 2 years. Despite individual shielding of the rectum, the rate of symptomatic proctitis rose from 1.7% to 5.6% in patients treated 1989 to 1992. CONCLUSIONS: We found no negative impact of decreasing the target volume on the overall and distant disease-free survival. The rate of symptomatic proctitis has increased with higher target doses despite better shielding of the rectum, but has remained within an acceptable range. Considering the high rate of biochemical relapse and therefore the poor prognosis associated with initial PSA values > 30 ng/ml, the application of a potentially toxic dose of > 70 Gy in these patients seems hardly justified.

Aged↗

[Validity of transrectal ultrasonography and echo-guided biopsy in the early diagnosis of prostatic neoplasms. Our experience on 100 cases].

100 patients from age 50 to 80 that presented urinary obstructive symptoms were studied with digital examination and with TR ultrasound; only 10 were palpably suspicious for prostatic carcinoma, the rest had a BPH. These 10 patients and 32 of the remaining revealed an abnormal area with ultrasound. 6 of the 10 palpably suspicious patients had prostatic carcinoma (60%). 8 of the 32 remaining patients had prostatic carcinoma (25%). Therefore of the 14 prostatic carcinoma 6 were of palpably positive patients, and 8 were of palpably negative patients. We suggest with this work that transrectal ultrasound is a useful technique in the early diagnosis and in the follow-up of prostatic nodules, and it is a necessary support in the decision for effecting echo-guided biopsy.

Aged↗

[Locoregional staging of prostatic neoplasms by extemporaneous histological examination of pelvic lymph nodes].

Pelvic lymphadenectomy prior to radical prostatectomy is essential to detect lymph nodal extension of prostatic cancer. Accuracy obtained by means of frozen section examination of the nodes is particularly favourable. Frozen sections correspond to paraffin sections in 100% of the cases. Authors' experience in 42 consecutive patients candidates to radical prostatic procedure is herein presented.

Adenocarcinoma↗

Flow and image cytometric DNA measurements in fine needle aspiration samples of prostatic neoplasms.

OBJECTIVE: To compare the DNA content measured by flow cytometry (FCM) and image analysis (IA) from prostatic fine needle aspiration (FNA) samples. STUDY DESIGN: A total of 48 samples were studied. FCM was performed on propidium iodide-stained nuclei according to the Vindelov method, and image analysis was performed on Feulgen-stained slides. Positive FNA results were grade (1-3) and compared with Gleason grades. RESULTS: Aneuploidy was closely related to positive FNA results (P < .01). DNA histograms were found to be aneuploid in all grade 3 carcinomas (n = 13) by IA and in 11 cases (84.6%) by FCM. Grade 2 carcinomas (n = 9) were found to be aneuploid with both methods. In grade 1 carcinomas (n = 10), 2 cases exhibited IA aneuploid profiles, whereas all FCM cases were diploid. Aneuploid profiles were more often associated with high Gleason scores than were diploid ones (P < .01). Among the 16 patients with negative FNA results, two cases had tetraploid DNA profiles related to contaminating seminal vesicle cells. The difference in DNA measurements reached 10.4% but was not statistically significant. CONCLUSION: These findings show that the two methods, as applied to prostatic FNA samples, give comparable results and that seminal vesicle cells may be responsible for false tetraploid profiles.

Biopsy, Needle↗

[Effect of neuroendocrine cell differentiation of the human prostatic neoplasm cell line LNCaP on the characteristics of volume-sensitive chloride content].

By means of patch-clamp technique we examined changes in volume-regulated chloride current (ICl,swell) at neuroendocrine differentiation of androgen-dependent LNCaP prostate cancer cells. In those cells with neuroendocrine differentiation resulted from an increase in the intracellular cAMP, ICl,swell became much faster in response to applying external hypotonic solution and cell swelling. Changes in final rectification and voltage-dependent inactivation were not detected, as compared to the control cells. The differentiation also diminished ICl,swell blockade by Ca2+ transported via store-operated channels (SOC). On the base of our data we suggest that potentiation of the current at neuroendocrine differentiation, at least in part, resulted from a decrease in an inhibitory effect of Ca2+, transported into a cell through SOC, on volume-sensitive chloride current. Accelerated current in those cells might be induced by cytoskeleton rearrangement at the neuron-like growth.

Calcium↗